Streptococcus satellite phage Javan198
Average proteome isoelectric point is 6.86
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 17 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZFL1|A0A4D5ZFL1_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan198 OX=2558561 GN=JavanS198_0010 PE=4 SV=1
MM1 pKa = 7.89 PEE3 pKa = 4.21 FIIVEE8 pKa = 4.28 GNNDD12 pKa = 3.55 LGEE15 pKa = 4.16 FFQIDD20 pKa = 3.85 GEE22 pKa = 4.35 LFSDD26 pKa = 4.83 NEE28 pKa = 4.0 LLEE31 pKa = 4.22 NLKK34 pKa = 10.0 KK35 pKa = 8.99 WRR37 pKa = 11.84 EE38 pKa = 3.87 WEE40 pKa = 4.17 VPVIIDD46 pKa = 3.52 DD47 pKa = 3.59 WCNRR51 pKa = 11.84 ILNEE55 pKa = 4.54 DD56 pKa = 3.71 EE57 pKa = 4.38 TEE59 pKa = 3.66 ILYY62 pKa = 10.57 FPTHH66 pKa = 6.28 EE67 pKa = 4.99 DD68 pKa = 2.87 KK69 pKa = 10.47 MDD71 pKa = 4.18 YY72 pKa = 10.59 IRR74 pKa = 11.84 VEE76 pKa = 3.94 KK77 pKa = 10.62 DD78 pKa = 3.22 LEE80 pKa = 4.29 PLYY83 pKa = 9.32 HH84 pKa = 6.59 TPNKK88 pKa = 10.12 PYY90 pKa = 9.26 VTISKK95 pKa = 10.3 SEE97 pKa = 3.86 WLEE100 pKa = 3.82 LLDD103 pKa = 4.57
Molecular weight: 12.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.269
IPC2_protein 4.139
IPC_protein 4.062
Toseland 3.884
ProMoST 4.126
Dawson 4.012
Bjellqvist 4.202
Wikipedia 3.897
Rodwell 3.897
Grimsley 3.795
Solomon 4.012
Lehninger 3.961
Nozaki 4.126
DTASelect 4.266
Thurlkill 3.91
EMBOSS 3.91
Sillero 4.177
Patrickios 3.554
IPC_peptide 4.012
IPC2_peptide 4.164
IPC2.peptide.svr19 4.094
Protein with the highest isoelectric point:
>tr|A0A4D5ZKE9|A0A4D5ZKE9_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan198 OX=2558561 GN=JavanS198_0009 PE=4 SV=1
MM1 pKa = 7.47 GKK3 pKa = 10.26 AISVKK8 pKa = 9.64 IDD10 pKa = 3.08 LGGIEE15 pKa = 5.09 KK16 pKa = 10.53 KK17 pKa = 10.67 LSPQSLQIGKK27 pKa = 9.15 VAMMNQMMLDD37 pKa = 3.6 TNKK40 pKa = 10.51 YY41 pKa = 8.93 IPKK44 pKa = 10.16 KK45 pKa = 10.22 SGNLRR50 pKa = 11.84 ASGHH54 pKa = 4.76 VVGNSIVYY62 pKa = 8.73 NPPYY66 pKa = 10.82 ARR68 pKa = 11.84 AQYY71 pKa = 9.87 YY72 pKa = 9.11 GPKK75 pKa = 9.84 RR76 pKa = 11.84 KK77 pKa = 10.12 GFVSEE82 pKa = 3.75 KK83 pKa = 8.64 QRR85 pKa = 11.84 RR86 pKa = 11.84 FFFANMEE93 pKa = 4.08 EE94 pKa = 4.49 LIAARR99 pKa = 11.84 RR100 pKa = 11.84 KK101 pKa = 8.56 YY102 pKa = 5.99 TTPGTGTHH110 pKa = 6.01 WYY112 pKa = 8.64 EE113 pKa = 4.76 KK114 pKa = 10.65 SFKK117 pKa = 10.03 NANNIRR123 pKa = 11.84 NWKK126 pKa = 9.65 RR127 pKa = 11.84 VFIKK131 pKa = 10.49 AIHH134 pKa = 6.64 IDD136 pKa = 3.4 ATT138 pKa = 3.8
Molecular weight: 15.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.24
IPC2_protein 9.882
IPC_protein 10.131
Toseland 10.599
ProMoST 10.175
Dawson 10.716
Bjellqvist 10.335
Wikipedia 10.862
Rodwell 11.286
Grimsley 10.76
Solomon 10.745
Lehninger 10.73
Nozaki 10.555
DTASelect 10.335
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.994
IPC_peptide 10.745
IPC2_peptide 8.902
IPC2.peptide.svr19 8.706
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17
0
17
2755
55
401
162.1
18.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.735 ± 0.517
0.508 ± 0.097
5.699 ± 0.371
8.385 ± 0.842
3.63 ± 0.379
4.574 ± 0.322
1.488 ± 0.198
7.586 ± 0.919
9.728 ± 0.583
9.111 ± 0.507
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.868 ± 0.323
6.207 ± 0.376
2.65 ± 0.285
4.791 ± 0.39
4.138 ± 0.351
5.808 ± 0.728
5.735 ± 0.543
5.953 ± 0.465
1.162 ± 0.214
4.247 ± 0.467
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here