Amino acid dipepetide frequency for Puccinia graminis f. sp. tritici

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.848AlaAla: 5.848 ± 0.027	0.967AlaCys: 0.967 ± 0.009	3.422AlaAsp: 3.422 ± 0.018	3.967AlaGlu: 3.967 ± 0.02	2.413AlaPhe: 2.413 ± 0.014	4.264AlaGly: 4.264 ± 0.021	1.893AlaHis: 1.893 ± 0.012	3.71AlaIle: 3.71 ± 0.017	3.612AlaLys: 3.612 ± 0.019	6.293AlaLeu: 6.293 ± 0.025	1.412AlaMet: 1.412 ± 0.011	2.934AlaAsn: 2.934 ± 0.017	4.439AlaPro: 4.439 ± 0.03	3.134AlaGln: 3.134 ± 0.017	3.994AlaArg: 3.994 ± 0.017	6.868AlaSer: 6.868 ± 0.023	4.27AlaThr: 4.27 ± 0.018	3.546AlaVal: 3.546 ± 0.018	0.794AlaTrp: 0.794 ± 0.008	1.567AlaTyr: 1.567 ± 0.011	0.0AlaXaa: 0.0 ± 0.0
Cys	0.807CysAla: 0.807 ± 0.009	0.292CysCys: 0.292 ± 0.005	0.647CysAsp: 0.647 ± 0.007	0.621CysGlu: 0.621 ± 0.007	0.604CysPhe: 0.604 ± 0.007	0.903CysGly: 0.903 ± 0.009	0.404CysHis: 0.404 ± 0.007	0.603CysIle: 0.603 ± 0.006	0.68CysLys: 0.68 ± 0.008	1.397CysLeu: 1.397 ± 0.012	0.272CysMet: 0.272 ± 0.005	0.498CysAsn: 0.498 ± 0.006	0.914CysPro: 0.914 ± 0.01	0.662CysGln: 0.662 ± 0.008	0.82CysArg: 0.82 ± 0.009	1.287CysSer: 1.287 ± 0.011	0.717CysThr: 0.717 ± 0.008	0.655CysVal: 0.655 ± 0.008	0.194CysTrp: 0.194 ± 0.003	0.361CysTyr: 0.361 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	2.956AspAla: 2.956 ± 0.017	0.75AspCys: 0.75 ± 0.008	3.866AspAsp: 3.866 ± 0.026	3.991AspGlu: 3.991 ± 0.027	2.03AspPhe: 2.03 ± 0.012	3.348AspGly: 3.348 ± 0.022	1.84AspHis: 1.84 ± 0.013	2.305AspIle: 2.305 ± 0.014	2.239AspLys: 2.239 ± 0.016	5.355AspLeu: 5.355 ± 0.023	0.894AspMet: 0.894 ± 0.01	1.908AspAsn: 1.908 ± 0.014	3.951AspPro: 3.951 ± 0.019	2.962AspGln: 2.962 ± 0.019	3.064AspArg: 3.064 ± 0.021	4.817AspSer: 4.817 ± 0.022	2.3AspThr: 2.3 ± 0.016	2.512AspVal: 2.512 ± 0.015	0.803AspTrp: 0.803 ± 0.008	1.407AspTyr: 1.407 ± 0.012	0.0AspXaa: 0.0 ± 0.0
Glu	4.314GluAla: 4.314 ± 0.021	0.613GluCys: 0.613 ± 0.007	3.857GluAsp: 3.857 ± 0.023	5.398GluGlu: 5.398 ± 0.038	1.961GluPhe: 1.961 ± 0.012	2.938GluGly: 2.938 ± 0.017	1.338GluHis: 1.338 ± 0.01	3.26GluIle: 3.26 ± 0.021	3.48GluLys: 3.48 ± 0.024	5.559GluLeu: 5.559 ± 0.028	1.259GluMet: 1.259 ± 0.01	2.529GluAsn: 2.529 ± 0.016	2.972GluPro: 2.972 ± 0.019	2.408GluGln: 2.408 ± 0.017	3.293GluArg: 3.293 ± 0.019	4.675GluSer: 4.675 ± 0.023	3.047GluThr: 3.047 ± 0.017	2.978GluVal: 2.978 ± 0.019	0.693GluTrp: 0.693 ± 0.008	1.34GluTyr: 1.34 ± 0.009	0.0GluXaa: 0.0 ± 0.0
Phe	2.149PheAla: 2.149 ± 0.013	0.597PheCys: 0.597 ± 0.006	2.166PheAsp: 2.166 ± 0.014	2.086PheGlu: 2.086 ± 0.013	1.665PhePhe: 1.665 ± 0.013	2.406PheGly: 2.406 ± 0.016	1.054PheHis: 1.054 ± 0.009	1.905PheIle: 1.905 ± 0.014	1.994PheLys: 1.994 ± 0.015	3.642PheLeu: 3.642 ± 0.018	0.706PheMet: 0.706 ± 0.008	1.753PheAsn: 1.753 ± 0.012	2.009PhePro: 2.009 ± 0.015	1.694PheGln: 1.694 ± 0.014	1.892PheArg: 1.892 ± 0.011	3.308PheSer: 3.308 ± 0.019	1.903PheThr: 1.903 ± 0.013	1.978PheVal: 1.978 ± 0.012	0.502PheTrp: 0.502 ± 0.007	0.992PheTyr: 0.992 ± 0.01	0.0PheXaa: 0.0 ± 0.0
Gly	3.572GlyAla: 3.572 ± 0.019	0.856GlyCys: 0.856 ± 0.011	2.573GlyAsp: 2.573 ± 0.015	2.703GlyGlu: 2.703 ± 0.015	2.445GlyPhe: 2.445 ± 0.015	4.501GlyGly: 4.501 ± 0.047	1.541GlyHis: 1.541 ± 0.013	2.957GlyIle: 2.957 ± 0.017	3.058GlyLys: 3.058 ± 0.02	5.448GlyLeu: 5.448 ± 0.024	1.152GlyMet: 1.152 ± 0.011	2.343GlyAsn: 2.343 ± 0.018	3.243GlyPro: 3.243 ± 0.017	2.401GlyGln: 2.401 ± 0.016	3.301GlyArg: 3.301 ± 0.019	5.622GlySer: 5.622 ± 0.026	3.22GlyThr: 3.22 ± 0.018	2.895GlyVal: 2.895 ± 0.018	0.831GlyTrp: 0.831 ± 0.01	1.609GlyTyr: 1.609 ± 0.015	0.0GlyXaa: 0.0 ± 0.0
His	1.523HisAla: 1.523 ± 0.01	0.405HisCys: 0.405 ± 0.006	1.284HisAsp: 1.284 ± 0.011	1.366HisGlu: 1.366 ± 0.01	1.098HisPhe: 1.098 ± 0.011	1.374HisGly: 1.374 ± 0.011	1.987HisHis: 1.987 ± 0.024	1.239HisIle: 1.239 ± 0.01	1.16HisLys: 1.16 ± 0.01	3.039HisLeu: 3.039 ± 0.018	0.469HisMet: 0.469 ± 0.006	1.125HisAsn: 1.125 ± 0.009	2.802HisPro: 2.802 ± 0.021	2.139HisGln: 2.139 ± 0.016	1.811HisArg: 1.811 ± 0.013	2.931HisSer: 2.931 ± 0.016	1.436HisThr: 1.436 ± 0.011	1.246HisVal: 1.246 ± 0.009	0.333HisTrp: 0.333 ± 0.005	0.73HisTyr: 0.73 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	3.248IleAla: 3.248 ± 0.015	0.865IleCys: 0.865 ± 0.009	3.138IleAsp: 3.138 ± 0.017	3.152IleGlu: 3.152 ± 0.018	2.003IlePhe: 2.003 ± 0.015	2.975IleGly: 2.975 ± 0.017	1.54IleHis: 1.54 ± 0.012	2.721IleIle: 2.721 ± 0.016	2.956IleLys: 2.956 ± 0.017	4.886IleLeu: 4.886 ± 0.025	0.905IleMet: 0.905 ± 0.009	2.475IleAsn: 2.475 ± 0.015	3.426IlePro: 3.426 ± 0.017	2.49IleGln: 2.49 ± 0.015	2.94IleArg: 2.94 ± 0.016	4.736IleSer: 4.736 ± 0.019	2.821IleThr: 2.821 ± 0.016	2.658IleVal: 2.658 ± 0.016	0.624IleTrp: 0.624 ± 0.007	1.304IleTyr: 1.304 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	3.957LysAla: 3.957 ± 0.019	0.611LysCys: 0.611 ± 0.008	2.74LysAsp: 2.74 ± 0.019	3.42LysGlu: 3.42 ± 0.022	1.859LysPhe: 1.859 ± 0.013	2.432LysGly: 2.432 ± 0.014	1.29LysHis: 1.29 ± 0.01	3.008LysIle: 3.008 ± 0.017	4.392LysLys: 4.392 ± 0.033	5.204LysLeu: 5.204 ± 0.025	1.176LysMet: 1.176 ± 0.01	2.533LysAsn: 2.533 ± 0.017	3.474LysPro: 3.474 ± 0.022	2.322LysGln: 2.322 ± 0.016	3.38LysArg: 3.38 ± 0.018	4.65LysSer: 4.65 ± 0.021	3.372LysThr: 3.372 ± 0.02	2.553LysVal: 2.553 ± 0.016	0.607LysTrp: 0.607 ± 0.007	1.266LysTyr: 1.266 ± 0.01	0.0LysXaa: 0.0 ± 0.0
Leu	7.153LeuAla: 7.153 ± 0.025	1.245LeuCys: 1.245 ± 0.011	5.484LeuAsp: 5.484 ± 0.025	5.659LeuGlu: 5.659 ± 0.027	3.396LeuPhe: 3.396 ± 0.019	5.259LeuGly: 5.259 ± 0.023	2.422LeuHis: 2.422 ± 0.013	5.17LeuIle: 5.17 ± 0.023	5.517LeuLys: 5.517 ± 0.023	8.865LeuLeu: 8.865 ± 0.037	1.871LeuMet: 1.871 ± 0.013	4.311LeuAsn: 4.311 ± 0.02	6.229LeuPro: 6.229 ± 0.026	3.989LeuGln: 3.989 ± 0.019	5.071LeuArg: 5.071 ± 0.024	8.783LeuSer: 8.783 ± 0.031	5.166LeuThr: 5.166 ± 0.022	5.276LeuVal: 5.276 ± 0.02	1.04LeuTrp: 1.04 ± 0.009	2.048LeuTyr: 2.048 ± 0.014	0.0LeuXaa: 0.0 ± 0.0
Met	1.633MetAla: 1.633 ± 0.011	0.245MetCys: 0.245 ± 0.004	1.121MetAsp: 1.121 ± 0.01	1.126MetGlu: 1.126 ± 0.011	0.7MetPhe: 0.7 ± 0.008	1.103MetGly: 1.103 ± 0.011	0.397MetHis: 0.397 ± 0.006	1.26MetIle: 1.26 ± 0.011	1.228MetLys: 1.228 ± 0.01	1.543MetLeu: 1.543 ± 0.009	0.584MetMet: 0.584 ± 0.007	0.983MetAsn: 0.983 ± 0.009	0.953MetPro: 0.953 ± 0.01	0.667MetGln: 0.667 ± 0.008	1.04MetArg: 1.04 ± 0.01	1.794MetSer: 1.794 ± 0.012	1.166MetThr: 1.166 ± 0.011	1.126MetVal: 1.126 ± 0.009	0.219MetTrp: 0.219 ± 0.004	0.438MetTyr: 0.438 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.443AsnAla: 2.443 ± 0.016	0.603AsnCys: 0.603 ± 0.007	2.096AsnAsp: 2.096 ± 0.014	2.299AsnGlu: 2.299 ± 0.013	1.636AsnPhe: 1.636 ± 0.011	2.542AsnGly: 2.542 ± 0.017	1.756AsnHis: 1.756 ± 0.014	1.991AsnIle: 1.991 ± 0.012	2.163AsnLys: 2.163 ± 0.014	4.494AsnLeu: 4.494 ± 0.019	0.771AsnMet: 0.771 ± 0.008	2.67AsnAsn: 2.67 ± 0.027	3.803AsnPro: 3.803 ± 0.018	2.889AsnGln: 2.889 ± 0.017	2.547AsnArg: 2.547 ± 0.015	4.362AsnSer: 4.362 ± 0.023	2.427AsnThr: 2.427 ± 0.015	1.918AsnVal: 1.918 ± 0.012	0.561AsnTrp: 0.561 ± 0.006	1.182AsnTyr: 1.182 ± 0.011	0.0AsnXaa: 0.0 ± 0.0
Pro	5.369ProAla: 5.369 ± 0.03	0.635ProCys: 0.635 ± 0.01	3.271ProAsp: 3.271 ± 0.018	3.606ProGlu: 3.606 ± 0.019	2.07ProPhe: 2.07 ± 0.013	3.554ProGly: 3.554 ± 0.022	1.954ProHis: 1.954 ± 0.015	3.479ProIle: 3.479 ± 0.017	3.288ProLys: 3.288 ± 0.019	5.39ProLeu: 5.39 ± 0.021	1.145ProMet: 1.145 ± 0.01	3.203ProAsn: 3.203 ± 0.02	6.686ProPro: 6.686 ± 0.049	2.959ProGln: 2.959 ± 0.026	3.374ProArg: 3.374 ± 0.021	8.595ProSer: 8.595 ± 0.04	5.2ProThr: 5.2 ± 0.024	3.518ProVal: 3.518 ± 0.021	0.593ProTrp: 0.593 ± 0.007	1.24ProTyr: 1.24 ± 0.01	0.0ProXaa: 0.0 ± 0.0
Gln	3.807GlnAla: 3.807 ± 0.02	0.481GlnCys: 0.481 ± 0.007	2.339GlnAsp: 2.339 ± 0.015	2.795GlnGlu: 2.795 ± 0.019	1.507GlnPhe: 1.507 ± 0.011	2.025GlnGly: 2.025 ± 0.014	1.474GlnHis: 1.474 ± 0.012	2.46GlnIle: 2.46 ± 0.013	2.429GlnLys: 2.429 ± 0.015	4.697GlnLeu: 4.697 ± 0.024	0.902GlnMet: 0.902 ± 0.009	2.058GlnAsn: 2.058 ± 0.016	3.671GlnPro: 3.671 ± 0.023	3.593GlnGln: 3.593 ± 0.055	2.463GlnArg: 2.463 ± 0.015	4.538GlnSer: 4.538 ± 0.02	2.909GlnThr: 2.909 ± 0.017	2.351GlnVal: 2.351 ± 0.014	0.48GlnTrp: 0.48 ± 0.006	0.958GlnTyr: 0.958 ± 0.011	0.0GlnXaa: 0.0 ± 0.0
Arg	3.966ArgAla: 3.966 ± 0.019	0.779ArgCys: 0.779 ± 0.009	2.53ArgAsp: 2.53 ± 0.017	2.9ArgGlu: 2.9 ± 0.018	2.204ArgPhe: 2.204 ± 0.014	2.805ArgGly: 2.805 ± 0.02	1.5ArgHis: 1.5 ± 0.013	2.971ArgIle: 2.971 ± 0.017	3.458ArgLys: 3.458 ± 0.02	5.789ArgLeu: 5.789 ± 0.027	1.183ArgMet: 1.183 ± 0.009	2.321ArgAsn: 2.321 ± 0.015	3.817ArgPro: 3.817 ± 0.023	2.554ArgGln: 2.554 ± 0.016	4.257ArgArg: 4.257 ± 0.027	5.483ArgSer: 5.483 ± 0.026	3.223ArgThr: 3.223 ± 0.018	2.797ArgVal: 2.797 ± 0.016	0.754ArgTrp: 0.754 ± 0.008	1.373ArgTyr: 1.373 ± 0.01	0.0ArgXaa: 0.0 ± 0.0
Ser	6.692SerAla: 6.692 ± 0.025	1.19SerCys: 1.19 ± 0.011	4.895SerAsp: 4.895 ± 0.021	4.7SerGlu: 4.7 ± 0.023	3.455SerPhe: 3.455 ± 0.018	5.258SerGly: 5.258 ± 0.025	2.902SerHis: 2.902 ± 0.017	5.057SerIle: 5.057 ± 0.021	5.023SerLys: 5.023 ± 0.024	8.722SerLeu: 8.722 ± 0.032	1.757SerMet: 1.757 ± 0.011	4.867SerAsn: 4.867 ± 0.026	6.891SerPro: 6.891 ± 0.039	4.585SerGln: 4.585 ± 0.023	5.429SerArg: 5.429 ± 0.028	14.246SerSer: 14.246 ± 0.084	7.105SerThr: 7.105 ± 0.034	4.452SerVal: 4.452 ± 0.02	0.968SerTrp: 0.968 ± 0.009	2.01SerTyr: 2.01 ± 0.014	0.0SerXaa: 0.0 ± 0.0
Thr	4.149ThrAla: 4.149 ± 0.021	0.844ThrCys: 0.844 ± 0.007	2.81ThrAsp: 2.81 ± 0.015	2.978ThrGlu: 2.978 ± 0.018	1.964ThrPhe: 1.964 ± 0.013	3.46ThrGly: 3.46 ± 0.02	1.712ThrHis: 1.712 ± 0.012	3.189ThrIle: 3.189 ± 0.017	2.94ThrLys: 2.94 ± 0.014	5.239ThrLeu: 5.239 ± 0.023	1.068ThrMet: 1.068 ± 0.01	2.851ThrAsn: 2.851 ± 0.016	4.636ThrPro: 4.636 ± 0.027	2.629ThrGln: 2.629 ± 0.015	3.327ThrArg: 3.327 ± 0.017	6.305ThrSer: 6.305 ± 0.028	5.012ThrThr: 5.012 ± 0.034	2.86ThrVal: 2.86 ± 0.016	0.677ThrTrp: 0.677 ± 0.008	1.297ThrTyr: 1.297 ± 0.012	0.0ThrXaa: 0.0 ± 0.0
Val	3.601ValAla: 3.601 ± 0.021	0.778ValCys: 0.778 ± 0.009	3.121ValAsp: 3.121 ± 0.014	3.236ValGlu: 3.236 ± 0.017	1.919ValPhe: 1.919 ± 0.011	3.023ValGly: 3.023 ± 0.019	1.351ValHis: 1.351 ± 0.01	2.737ValIle: 2.737 ± 0.015	2.701ValLys: 2.701 ± 0.017	4.64ValLeu: 4.64 ± 0.022	1.018ValMet: 1.018 ± 0.009	2.073ValAsn: 2.073 ± 0.013	3.155ValPro: 3.155 ± 0.016	2.153ValGln: 2.153 ± 0.013	2.572ValArg: 2.572 ± 0.017	4.216ValSer: 4.216 ± 0.016	2.747ValThr: 2.747 ± 0.017	3.085ValVal: 3.085 ± 0.017	0.654ValTrp: 0.654 ± 0.007	1.329ValTyr: 1.329 ± 0.01	0.0ValXaa: 0.0 ± 0.0
Trp	0.812TrpAla: 0.812 ± 0.008	0.187TrpCys: 0.187 ± 0.004	0.668TrpAsp: 0.668 ± 0.008	0.682TrpGlu: 0.682 ± 0.007	0.446TrpPhe: 0.446 ± 0.006	0.625TrpGly: 0.625 ± 0.008	0.278TrpHis: 0.278 ± 0.005	0.725TrpIle: 0.725 ± 0.008	0.84TrpLys: 0.84 ± 0.007	1.176TrpLeu: 1.176 ± 0.01	0.299TrpMet: 0.299 ± 0.005	0.661TrpAsn: 0.661 ± 0.008	0.515TrpPro: 0.515 ± 0.006	0.45TrpGln: 0.45 ± 0.006	0.695TrpArg: 0.695 ± 0.009	0.973TrpSer: 0.973 ± 0.01	0.724TrpThr: 0.724 ± 0.008	0.588TrpVal: 0.588 ± 0.007	0.189TrpTrp: 0.189 ± 0.005	0.314TrpTyr: 0.314 ± 0.005	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.347TyrAla: 1.347 ± 0.011	0.397TyrCys: 0.397 ± 0.005	1.27TyrAsp: 1.27 ± 0.011	1.23TyrGlu: 1.23 ± 0.01	1.025TyrPhe: 1.025 ± 0.008	1.476TyrGly: 1.476 ± 0.015	0.866TyrHis: 0.866 ± 0.01	1.118TyrIle: 1.118 ± 0.009	1.087TyrLys: 1.087 ± 0.009	2.576TyrLeu: 2.576 ± 0.014	0.479TyrMet: 0.479 ± 0.006	1.057TyrAsn: 1.057 ± 0.01	1.543TyrPro: 1.543 ± 0.012	1.257TyrGln: 1.257 ± 0.009	1.393TyrArg: 1.393 ± 0.013	2.013TyrSer: 2.013 ± 0.013	1.186TyrThr: 1.186 ± 0.013	1.117TyrVal: 1.117 ± 0.009	0.331TyrTrp: 0.331 ± 0.005	0.794TyrTyr: 0.794 ± 0.008	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 36348 proteins (13159220 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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