Amino acid dipepetide frequency for Proteus phage vB_PmiM_Pm5461

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.525AlaAla: 2.525 ± 0.267
0.311AlaCys: 0.311 ± 0.064
2.545AlaAsp: 2.545 ± 0.244
3.438AlaGlu: 3.438 ± 0.314
2.176AlaPhe: 2.176 ± 0.181
2.642AlaGly: 2.642 ± 0.302
0.68AlaHis: 0.68 ± 0.118
4.177AlaIle: 4.177 ± 0.273
4.449AlaLys: 4.449 ± 0.265
4.74AlaLeu: 4.74 ± 0.325
1.049AlaMet: 1.049 ± 0.149
2.292AlaAsn: 2.292 ± 0.223
1.399AlaPro: 1.399 ± 0.157
1.535AlaGln: 1.535 ± 0.164
1.729AlaArg: 1.729 ± 0.182
3.73AlaSer: 3.73 ± 0.299
2.156AlaThr: 2.156 ± 0.261
2.506AlaVal: 2.506 ± 0.238
0.622AlaTrp: 0.622 ± 0.114
2.098AlaTyr: 2.098 ± 0.202
0.0AlaXaa: 0.0 ± 0.0
Cys
0.563CysAla: 0.563 ± 0.106
0.175CysCys: 0.175 ± 0.057
0.738CysAsp: 0.738 ± 0.128
0.932CysGlu: 0.932 ± 0.141
0.66CysPhe: 0.66 ± 0.118
0.641CysGly: 0.641 ± 0.131
0.253CysHis: 0.253 ± 0.071
0.932CysIle: 0.932 ± 0.134
0.991CysLys: 0.991 ± 0.131
0.758CysLeu: 0.758 ± 0.124
0.35CysMet: 0.35 ± 0.068
0.719CysAsn: 0.719 ± 0.115
0.447CysPro: 0.447 ± 0.105
0.291CysGln: 0.291 ± 0.072
0.524CysArg: 0.524 ± 0.091
1.068CysSer: 1.068 ± 0.165
0.583CysThr: 0.583 ± 0.1
0.796CysVal: 0.796 ± 0.14
0.019CysTrp: 0.019 ± 0.017
0.524CysTyr: 0.524 ± 0.122
0.0CysXaa: 0.0 ± 0.0
Asp
2.7AspAla: 2.7 ± 0.253
0.738AspCys: 0.738 ± 0.122
3.38AspAsp: 3.38 ± 0.307
4.313AspGlu: 4.313 ± 0.299
3.594AspPhe: 3.594 ± 0.276
3.71AspGly: 3.71 ± 0.278
0.932AspHis: 0.932 ± 0.124
5.905AspIle: 5.905 ± 0.34
5.653AspLys: 5.653 ± 0.374
5.925AspLeu: 5.925 ± 0.348
1.632AspMet: 1.632 ± 0.149
3.671AspAsn: 3.671 ± 0.276
2.273AspPro: 2.273 ± 0.232
1.807AspGln: 1.807 ± 0.201
1.845AspArg: 1.845 ± 0.184
4.429AspSer: 4.429 ± 0.305
3.03AspThr: 3.03 ± 0.244
4.002AspVal: 4.002 ± 0.236
1.282AspTrp: 1.282 ± 0.18
3.069AspTyr: 3.069 ± 0.278
0.0AspXaa: 0.0 ± 0.0
Glu
4.254GluAla: 4.254 ± 0.339
0.894GluCys: 0.894 ± 0.12
4.332GluAsp: 4.332 ± 0.286
6.236GluGlu: 6.236 ± 0.42
3.866GluPhe: 3.866 ± 0.292
3.535GluGly: 3.535 ± 0.209
1.107GluHis: 1.107 ± 0.155
5.944GluIle: 5.944 ± 0.317
5.983GluLys: 5.983 ± 0.376
7.557GluLeu: 7.557 ± 0.431
1.904GluMet: 1.904 ± 0.198
4.332GluAsn: 4.332 ± 0.284
1.807GluPro: 1.807 ± 0.214
2.564GluGln: 2.564 ± 0.227
2.059GluArg: 2.059 ± 0.195
5.187GluSer: 5.187 ± 0.366
4.429GluThr: 4.429 ± 0.323
4.856GluVal: 4.856 ± 0.309
0.699GluTrp: 0.699 ± 0.125
4.06GluTyr: 4.06 ± 0.317
0.0GluXaa: 0.0 ± 0.0
Phe
2.079PheAla: 2.079 ± 0.19
0.524PheCys: 0.524 ± 0.095
3.341PheAsp: 3.341 ± 0.282
3.516PheGlu: 3.516 ± 0.253
1.651PhePhe: 1.651 ± 0.188
2.622PheGly: 2.622 ± 0.238
0.486PheHis: 0.486 ± 0.094
3.4PheIle: 3.4 ± 0.28
5.342PheLys: 5.342 ± 0.316
3.166PheLeu: 3.166 ± 0.294
1.34PheMet: 1.34 ± 0.175
3.458PheAsn: 3.458 ± 0.297
1.204PhePro: 1.204 ± 0.165
0.894PheGln: 0.894 ± 0.14
1.476PheArg: 1.476 ± 0.167
3.361PheSer: 3.361 ± 0.232
2.448PheThr: 2.448 ± 0.212
2.642PheVal: 2.642 ± 0.211
0.641PheTrp: 0.641 ± 0.116
2.001PheTyr: 2.001 ± 0.205
0.0PheXaa: 0.0 ± 0.0
Gly
2.37GlyAla: 2.37 ± 0.203
0.622GlyCys: 0.622 ± 0.108
3.516GlyAsp: 3.516 ± 0.311
3.633GlyGlu: 3.633 ± 0.259
2.467GlyPhe: 2.467 ± 0.232
2.856GlyGly: 2.856 ± 0.29
0.952GlyHis: 0.952 ± 0.138
4.895GlyIle: 4.895 ± 0.267
4.643GlyLys: 4.643 ± 0.287
4.915GlyLeu: 4.915 ± 0.332
1.496GlyMet: 1.496 ± 0.196
2.875GlyAsn: 2.875 ± 0.249
1.826GlyPro: 1.826 ± 0.2
2.098GlyGln: 2.098 ± 0.215
2.117GlyArg: 2.117 ± 0.227
4.099GlySer: 4.099 ± 0.228
3.788GlyThr: 3.788 ± 0.324
3.516GlyVal: 3.516 ± 0.262
0.835GlyTrp: 0.835 ± 0.146
2.933GlyTyr: 2.933 ± 0.205
0.0GlyXaa: 0.0 ± 0.0
His
0.699HisAla: 0.699 ± 0.105
0.175HisCys: 0.175 ± 0.062
0.913HisAsp: 0.913 ± 0.146
1.03HisGlu: 1.03 ± 0.14
0.816HisPhe: 0.816 ± 0.134
0.991HisGly: 0.991 ± 0.144
0.35HisHis: 0.35 ± 0.088
1.34HisIle: 1.34 ± 0.18
1.535HisLys: 1.535 ± 0.168
1.418HisLeu: 1.418 ± 0.19
0.466HisMet: 0.466 ± 0.093
1.03HisAsn: 1.03 ± 0.152
0.991HisPro: 0.991 ± 0.156
0.486HisGln: 0.486 ± 0.093
0.602HisArg: 0.602 ± 0.123
1.204HisSer: 1.204 ± 0.174
0.738HisThr: 0.738 ± 0.111
0.932HisVal: 0.932 ± 0.135
0.097HisTrp: 0.097 ± 0.04
0.855HisTyr: 0.855 ± 0.1
0.0HisXaa: 0.0 ± 0.0
Ile
3.769IleAla: 3.769 ± 0.323
1.146IleCys: 1.146 ± 0.171
5.672IleAsp: 5.672 ± 0.36
6.372IleGlu: 6.372 ± 0.424
3.38IlePhe: 3.38 ± 0.269
4.215IleGly: 4.215 ± 0.269
1.438IleHis: 1.438 ± 0.176
6.352IleIle: 6.352 ± 0.373
8.78IleLys: 8.78 ± 0.45
6.022IleLeu: 6.022 ± 0.357
1.884IleMet: 1.884 ± 0.198
5.517IleAsn: 5.517 ± 0.315
3.147IlePro: 3.147 ± 0.29
2.389IleGln: 2.389 ± 0.179
2.933IleArg: 2.933 ± 0.266
5.731IleSer: 5.731 ± 0.36
4.215IleThr: 4.215 ± 0.239
4.371IleVal: 4.371 ± 0.305
0.855IleTrp: 0.855 ± 0.142
3.128IleTyr: 3.128 ± 0.247
0.0IleXaa: 0.0 ± 0.0
Lys
4.623LysAla: 4.623 ± 0.299
1.01LysCys: 1.01 ± 0.152
6.391LysAsp: 6.391 ± 0.359
7.848LysGlu: 7.848 ± 0.436
4.332LysPhe: 4.332 ± 0.295
4.818LysGly: 4.818 ± 0.289
1.904LysHis: 1.904 ± 0.173
6.721LysIle: 6.721 ± 0.335
5.769LysLys: 5.769 ± 0.341
7.926LysLeu: 7.926 ± 0.405
2.273LysMet: 2.273 ± 0.27
5.226LysAsn: 5.226 ± 0.307
2.894LysPro: 2.894 ± 0.218
2.992LysGln: 2.992 ± 0.222
3.264LysArg: 3.264 ± 0.283
6.158LysSer: 6.158 ± 0.348
6.041LysThr: 6.041 ± 0.332
4.992LysVal: 4.992 ± 0.356
1.263LysTrp: 1.263 ± 0.153
4.74LysTyr: 4.74 ± 0.32
0.0LysXaa: 0.0 ± 0.0
Leu
3.943LeuAla: 3.943 ± 0.305
1.127LeuCys: 1.127 ± 0.188
5.497LeuAsp: 5.497 ± 0.346
6.993LeuGlu: 6.993 ± 0.421
3.128LeuPhe: 3.128 ± 0.257
4.876LeuGly: 4.876 ± 0.27
1.321LeuHis: 1.321 ± 0.168
5.575LeuIle: 5.575 ± 0.325
7.829LeuLys: 7.829 ± 0.469
6.003LeuLeu: 6.003 ± 0.324
2.04LeuMet: 2.04 ± 0.193
5.362LeuAsn: 5.362 ± 0.283
3.264LeuPro: 3.264 ± 0.244
2.331LeuGln: 2.331 ± 0.192
2.817LeuArg: 2.817 ± 0.218
6.411LeuSer: 6.411 ± 0.349
3.788LeuThr: 3.788 ± 0.261
4.643LeuVal: 4.643 ± 0.322
0.991LeuTrp: 0.991 ± 0.157
3.749LeuTyr: 3.749 ± 0.304
0.0LeuXaa: 0.0 ± 0.0
Met
1.457MetAla: 1.457 ± 0.146
0.175MetCys: 0.175 ± 0.051
1.573MetAsp: 1.573 ± 0.141
1.438MetGlu: 1.438 ± 0.173
1.36MetPhe: 1.36 ± 0.152
1.127MetGly: 1.127 ± 0.174
0.35MetHis: 0.35 ± 0.081
2.079MetIle: 2.079 ± 0.194
2.292MetLys: 2.292 ± 0.199
1.457MetLeu: 1.457 ± 0.161
0.369MetMet: 0.369 ± 0.086
1.807MetAsn: 1.807 ± 0.191
0.894MetPro: 0.894 ± 0.128
0.874MetGln: 0.874 ± 0.127
0.563MetArg: 0.563 ± 0.109
1.612MetSer: 1.612 ± 0.181
1.787MetThr: 1.787 ± 0.163
1.379MetVal: 1.379 ± 0.146
0.233MetTrp: 0.233 ± 0.069
1.146MetTyr: 1.146 ± 0.151
0.0MetXaa: 0.0 ± 0.0
Asn
2.409AsnAla: 2.409 ± 0.226
0.68AsnCys: 0.68 ± 0.126
3.302AsnAsp: 3.302 ± 0.215
3.769AsnGlu: 3.769 ± 0.246
2.642AsnPhe: 2.642 ± 0.228
4.138AsnGly: 4.138 ± 0.301
1.049AsnHis: 1.049 ± 0.143
5.323AsnIle: 5.323 ± 0.271
5.459AsnLys: 5.459 ± 0.41
5.012AsnLeu: 5.012 ± 0.321
1.554AsnMet: 1.554 ± 0.166
3.807AsnAsn: 3.807 ± 0.288
2.914AsnPro: 2.914 ± 0.224
1.768AsnGln: 1.768 ± 0.189
2.506AsnArg: 2.506 ± 0.216
4.313AsnSer: 4.313 ± 0.293
3.361AsnThr: 3.361 ± 0.214
3.38AsnVal: 3.38 ± 0.262
0.68AsnTrp: 0.68 ± 0.114
2.292AsnTyr: 2.292 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
1.224ProAla: 1.224 ± 0.179
0.466ProCys: 0.466 ± 0.121
2.428ProAsp: 2.428 ± 0.233
3.613ProGlu: 3.613 ± 0.276
1.748ProPhe: 1.748 ± 0.165
2.137ProGly: 2.137 ± 0.223
0.66ProHis: 0.66 ± 0.121
2.506ProIle: 2.506 ± 0.22
3.38ProLys: 3.38 ± 0.27
2.04ProLeu: 2.04 ± 0.178
0.622ProMet: 0.622 ± 0.106
1.962ProAsn: 1.962 ± 0.196
0.855ProPro: 0.855 ± 0.133
1.01ProGln: 1.01 ± 0.134
1.146ProArg: 1.146 ± 0.158
2.156ProSer: 2.156 ± 0.171
1.807ProThr: 1.807 ± 0.176
2.331ProVal: 2.331 ± 0.208
0.486ProTrp: 0.486 ± 0.089
1.554ProTyr: 1.554 ± 0.165
0.0ProXaa: 0.0 ± 0.0
Gln
1.535GlnAla: 1.535 ± 0.163
0.33GlnCys: 0.33 ± 0.082
1.651GlnAsp: 1.651 ± 0.163
2.914GlnGlu: 2.914 ± 0.245
1.496GlnPhe: 1.496 ± 0.143
2.059GlnGly: 2.059 ± 0.19
0.505GlnHis: 0.505 ± 0.092
2.758GlnIle: 2.758 ± 0.258
2.292GlnLys: 2.292 ± 0.241
2.195GlnLeu: 2.195 ± 0.21
0.796GlnMet: 0.796 ± 0.124
1.535GlnAsn: 1.535 ± 0.205
0.699GlnPro: 0.699 ± 0.119
1.01GlnGln: 1.01 ± 0.141
1.224GlnArg: 1.224 ± 0.13
1.865GlnSer: 1.865 ± 0.206
1.729GlnThr: 1.729 ± 0.22
2.195GlnVal: 2.195 ± 0.245
0.447GlnTrp: 0.447 ± 0.08
1.612GlnTyr: 1.612 ± 0.197
0.0GlnXaa: 0.0 ± 0.0
Arg
1.729ArgAla: 1.729 ± 0.212
0.389ArgCys: 0.389 ± 0.08
2.04ArgAsp: 2.04 ± 0.19
2.545ArgGlu: 2.545 ± 0.201
1.496ArgPhe: 1.496 ± 0.206
1.962ArgGly: 1.962 ± 0.214
0.777ArgHis: 0.777 ± 0.113
3.128ArgIle: 3.128 ± 0.235
2.972ArgLys: 2.972 ± 0.237
3.147ArgLeu: 3.147 ± 0.271
0.855ArgMet: 0.855 ± 0.13
2.137ArgAsn: 2.137 ± 0.209
0.932ArgPro: 0.932 ± 0.124
1.166ArgGln: 1.166 ± 0.153
1.379ArgArg: 1.379 ± 0.213
2.176ArgSer: 2.176 ± 0.199
2.02ArgThr: 2.02 ± 0.191
2.409ArgVal: 2.409 ± 0.244
0.486ArgTrp: 0.486 ± 0.1
1.554ArgTyr: 1.554 ± 0.183
0.0ArgXaa: 0.0 ± 0.0
Ser
2.778SerAla: 2.778 ± 0.251
1.01SerCys: 1.01 ± 0.165
4.992SerAsp: 4.992 ± 0.348
4.487SerGlu: 4.487 ± 0.316
3.108SerPhe: 3.108 ± 0.229
4.39SerGly: 4.39 ± 0.311
1.03SerHis: 1.03 ± 0.141
6.177SerIle: 6.177 ± 0.328
7.246SerLys: 7.246 ± 0.418
5.711SerLeu: 5.711 ± 0.352
1.709SerMet: 1.709 ± 0.204
4.313SerAsn: 4.313 ± 0.311
2.079SerPro: 2.079 ± 0.187
2.079SerGln: 2.079 ± 0.157
2.875SerArg: 2.875 ± 0.23
4.895SerSer: 4.895 ± 0.343
3.807SerThr: 3.807 ± 0.314
4.332SerVal: 4.332 ± 0.318
0.758SerTrp: 0.758 ± 0.129
2.758SerTyr: 2.758 ± 0.242
0.0SerXaa: 0.0 ± 0.0
Thr
2.312ThrAla: 2.312 ± 0.239
0.563ThrCys: 0.563 ± 0.105
3.264ThrAsp: 3.264 ± 0.253
4.196ThrGlu: 4.196 ± 0.271
2.253ThrPhe: 2.253 ± 0.222
3.419ThrGly: 3.419 ± 0.305
1.03ThrHis: 1.03 ± 0.146
4.72ThrIle: 4.72 ± 0.327
4.604ThrLys: 4.604 ± 0.259
4.837ThrLeu: 4.837 ± 0.329
1.01ThrMet: 1.01 ± 0.142
3.069ThrAsn: 3.069 ± 0.236
2.467ThrPro: 2.467 ± 0.23
1.768ThrGln: 1.768 ± 0.23
2.234ThrArg: 2.234 ± 0.237
3.613ThrSer: 3.613 ± 0.271
2.545ThrThr: 2.545 ± 0.285
3.283ThrVal: 3.283 ± 0.275
0.913ThrTrp: 0.913 ± 0.139
2.389ThrTyr: 2.389 ± 0.197
0.0ThrXaa: 0.0 ± 0.0
Val
2.7ValAla: 2.7 ± 0.241
0.816ValCys: 0.816 ± 0.106
4.177ValAsp: 4.177 ± 0.289
4.468ValGlu: 4.468 ± 0.281
2.817ValPhe: 2.817 ± 0.199
3.186ValGly: 3.186 ± 0.252
0.68ValHis: 0.68 ± 0.11
4.74ValIle: 4.74 ± 0.303
6.158ValLys: 6.158 ± 0.38
4.992ValLeu: 4.992 ± 0.317
1.204ValMet: 1.204 ± 0.13
3.341ValAsn: 3.341 ± 0.279
2.04ValPro: 2.04 ± 0.214
2.292ValGln: 2.292 ± 0.219
1.904ValArg: 1.904 ± 0.191
4.371ValSer: 4.371 ± 0.344
2.642ValThr: 2.642 ± 0.206
4.002ValVal: 4.002 ± 0.342
0.68ValTrp: 0.68 ± 0.124
3.05ValTyr: 3.05 ± 0.238
0.0ValXaa: 0.0 ± 0.0
Trp
0.874TrpAla: 0.874 ± 0.116
0.117TrpCys: 0.117 ± 0.048
1.107TrpAsp: 1.107 ± 0.131
0.932TrpGlu: 0.932 ± 0.135
0.466TrpPhe: 0.466 ± 0.105
0.505TrpGly: 0.505 ± 0.094
0.311TrpHis: 0.311 ± 0.096
1.01TrpIle: 1.01 ± 0.129
1.107TrpLys: 1.107 ± 0.148
0.66TrpLeu: 0.66 ± 0.134
0.408TrpMet: 0.408 ± 0.09
0.68TrpAsn: 0.68 ± 0.112
0.447TrpPro: 0.447 ± 0.091
0.272TrpGln: 0.272 ± 0.068
0.233TrpArg: 0.233 ± 0.062
0.894TrpSer: 0.894 ± 0.166
0.641TrpThr: 0.641 ± 0.106
1.068TrpVal: 1.068 ± 0.14
0.117TrpTrp: 0.117 ± 0.051
0.758TrpTyr: 0.758 ± 0.108
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.253TyrAla: 2.253 ± 0.204
0.699TyrCys: 0.699 ± 0.124
3.03TyrAsp: 3.03 ± 0.225
2.933TyrGlu: 2.933 ± 0.252
2.156TyrPhe: 2.156 ± 0.238
2.622TyrGly: 2.622 ± 0.204
0.855TyrHis: 0.855 ± 0.151
3.633TyrIle: 3.633 ± 0.276
4.701TyrLys: 4.701 ± 0.305
3.205TyrLeu: 3.205 ± 0.276
0.991TyrMet: 0.991 ± 0.114
3.225TyrAsn: 3.225 ± 0.256
1.554TyrPro: 1.554 ± 0.18
1.224TyrGln: 1.224 ± 0.171
1.865TyrArg: 1.865 ± 0.169
3.264TyrSer: 3.264 ± 0.262
2.933TyrThr: 2.933 ± 0.28
2.622TyrVal: 2.622 ± 0.203
0.505TyrTrp: 0.505 ± 0.086
2.079TyrTyr: 2.079 ± 0.234
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 256 proteins (51479 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski