Sphingomonas sp. HMWF008

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingomonas; unclassified Sphingomonas

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3537 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R7IN65|A0A2R7IN65_9SPHN UbiA family prenyltransferase OS=Sphingomonas sp. HMWF008 OX=2056845 GN=DBR17_17125 PE=4 SV=1
SS1 pKa = 4.87YY2 pKa = 9.85TFSVVVNGDD11 pKa = 3.47ALVEE15 pKa = 4.26PNEE18 pKa = 4.29TVLVNLSNVVGAAVTDD34 pKa = 3.98SQGSGTIIDD43 pKa = 3.92NDD45 pKa = 3.88FPTLSISDD53 pKa = 3.8ASVSEE58 pKa = 4.35GDD60 pKa = 3.35SGTTVLTYY68 pKa = 10.13TVTASAPALAGGISFTIATADD89 pKa = 3.48GTATAGSDD97 pKa = 3.29YY98 pKa = 10.83DD99 pKa = 3.86AQSTTGTIAEE109 pKa = 4.4GQTTYY114 pKa = 10.66TFSVVVNGDD123 pKa = 3.13VSNEE127 pKa = 4.0SNEE130 pKa = 4.24TVLVNLSNVTGATLVDD146 pKa = 3.68AQGVGTIVNDD156 pKa = 4.17DD157 pKa = 3.49GPIRR161 pKa = 11.84IFSEE165 pKa = 4.14SFSNFLAAGFAPTPSASQLDD185 pKa = 3.71SDD187 pKa = 3.82IWRR190 pKa = 11.84VAGLSDD196 pKa = 4.13NLTPAYY202 pKa = 10.0GATLTTGDD210 pKa = 3.88FARR213 pKa = 11.84GVINGAADD221 pKa = 3.59PTSGGVYY228 pKa = 10.14SPSANRR234 pKa = 11.84ALVVQPTGSDD244 pKa = 3.19FDD246 pKa = 3.98AGGFIEE252 pKa = 5.95AKK254 pKa = 9.43IQNSSGKK261 pKa = 7.61TAVAFDD267 pKa = 3.92VGFDD271 pKa = 3.0WAYY274 pKa = 10.6RR275 pKa = 11.84NSGNRR280 pKa = 11.84ADD282 pKa = 4.92NMQFSYY288 pKa = 9.61STDD291 pKa = 2.97GTNFTVVPAAAFVTPAAADD310 pKa = 3.47ASVAATFSNQNEE322 pKa = 4.61TVSLTGLAVAAGGFLYY338 pKa = 10.68LRR340 pKa = 11.84WTHH343 pKa = 6.86LDD345 pKa = 3.21GATGSGNRR353 pKa = 11.84DD354 pKa = 3.33EE355 pKa = 5.49IGIDD359 pKa = 3.23NVTIDD364 pKa = 3.64ATTSNLATATVANISLAEE382 pKa = 4.24GDD384 pKa = 4.3AGTTSATFTVVRR396 pKa = 11.84SSGVGAASIDD406 pKa = 3.73YY407 pKa = 7.65TTANGSAIAGTDD419 pKa = 3.46YY420 pKa = 10.96VAASGTVSFADD431 pKa = 3.92GEE433 pKa = 4.53TSQTVTILITGDD445 pKa = 3.33TAFEE449 pKa = 4.18AGEE452 pKa = 4.35TFFLNLSNPSNVLLPSSQASVTITNDD478 pKa = 3.02DD479 pKa = 3.66NGAVAIYY486 pKa = 9.47DD487 pKa = 3.55IQGAGHH493 pKa = 6.58TSALVGQFIQTSGIVTAVAANGFYY517 pKa = 10.83LQDD520 pKa = 3.49PTGDD524 pKa = 3.5GNTRR528 pKa = 11.84TSDD531 pKa = 3.75GIFVFTSTAPTVVVGNGISLTGTVTEE557 pKa = 5.06FDD559 pKa = 3.91AGLDD563 pKa = 3.78TLTVTQITAPTITVLTQTNTLPNAVLIGATGGILPPTQSIDD604 pKa = 3.3SDD606 pKa = 3.66NFTVFNPEE614 pKa = 2.83VDD616 pKa = 4.59GIDD619 pKa = 3.57FYY621 pKa = 11.46EE622 pKa = 4.26TLEE625 pKa = 4.08GMRR628 pKa = 11.84VTVQAPTVTSNTNSFGEE645 pKa = 4.32TFIVASGGIGATGVNEE661 pKa = 4.06RR662 pKa = 11.84GGITISAGDD671 pKa = 3.91NNPEE675 pKa = 4.77RR676 pKa = 11.84IQIDD680 pKa = 3.39NDD682 pKa = 3.63TTIFAGYY689 pKa = 7.75TPSHH693 pKa = 5.72TVGDD697 pKa = 3.81RR698 pKa = 11.84LGDD701 pKa = 3.4VTGVFNYY708 pKa = 10.25AFQSYY713 pKa = 8.46EE714 pKa = 4.28LIVTEE719 pKa = 4.37TVVVTNDD726 pKa = 3.26TTPTRR731 pKa = 11.84EE732 pKa = 4.04TTTLAGDD739 pKa = 3.62ATHH742 pKa = 5.97VTFASFNIEE751 pKa = 4.04NADD754 pKa = 3.6PTDD757 pKa = 3.97PASKK761 pKa = 8.98FTLLGTEE768 pKa = 3.57IRR770 pKa = 11.84YY771 pKa = 9.35ALNTPDD777 pKa = 4.83IIALQEE783 pKa = 3.93VQDD786 pKa = 4.09ADD788 pKa = 3.93GAGTGTNYY796 pKa = 10.61SGTVTAQKK804 pKa = 10.71LIDD807 pKa = 4.78AIIAAGGPTYY817 pKa = 10.61SYY819 pKa = 11.28IEE821 pKa = 4.08IAPTANNTTGGEE833 pKa = 4.06PNGNIRR839 pKa = 11.84NGFLYY844 pKa = 10.2NAARR848 pKa = 11.84VTYY851 pKa = 10.35VDD853 pKa = 3.54GSVALVPGIAYY864 pKa = 10.04NNSRR868 pKa = 11.84NPLVADD874 pKa = 4.74FSFNGQVFTTVSVHH888 pKa = 4.12STSRR892 pKa = 11.84GGSDD896 pKa = 3.47AFYY899 pKa = 11.29GDD901 pKa = 3.42IQPPVQAGDD910 pKa = 3.75SQRR913 pKa = 11.84TAQADD918 pKa = 3.69TLRR921 pKa = 11.84AYY923 pKa = 9.89INDD926 pKa = 3.69QLATDD931 pKa = 4.82PNHH934 pKa = 5.82QFIIGGDD941 pKa = 3.61FNGYY945 pKa = 9.52YY946 pKa = 9.9YY947 pKa = 8.81EE948 pKa = 4.1TAMQRR953 pKa = 11.84LVAGGVLTNLYY964 pKa = 10.5DD965 pKa = 4.21LLPSEE970 pKa = 4.63EE971 pKa = 4.23RR972 pKa = 11.84YY973 pKa = 10.47SYY975 pKa = 11.02LFEE978 pKa = 5.53GNAQAFDD985 pKa = 4.42NILVSGGLRR994 pKa = 11.84NNAQFDD1000 pKa = 4.1VVHH1003 pKa = 6.21YY1004 pKa = 10.73NSEE1007 pKa = 3.86QLAANQATDD1016 pKa = 3.14HH1017 pKa = 6.59DD1018 pKa = 4.4QVVARR1023 pKa = 11.84IEE1025 pKa = 3.96IPLPNVAPSGADD1037 pKa = 3.02ASRR1040 pKa = 11.84TILEE1044 pKa = 4.36DD1045 pKa = 3.1ASYY1048 pKa = 10.11TFATADD1054 pKa = 3.51FGFADD1059 pKa = 4.1GNGNALSGVRR1069 pKa = 11.84ITTLPGAGTLFLDD1082 pKa = 4.45ADD1084 pKa = 4.34GAGGNAPVVVTAGQTISAADD1104 pKa = 3.3IAAGKK1109 pKa = 8.56LTFVPAADD1117 pKa = 4.18GNGAGYY1123 pKa = 10.94GSFTFQVQDD1132 pKa = 4.29DD1133 pKa = 4.35GGTVNGGVNLDD1144 pKa = 3.41QSANSFTVNVTAQDD1158 pKa = 3.85DD1159 pKa = 4.24APTAVADD1166 pKa = 3.86VVTTAEE1172 pKa = 4.1NIIAVVNVVGNDD1184 pKa = 3.15TDD1186 pKa = 3.76IDD1188 pKa = 4.05GGPKK1192 pKa = 10.3GVVSVNGNALTSGQSTTLASGAIVTLGDD1220 pKa = 4.51DD1221 pKa = 3.4GTLRR1225 pKa = 11.84YY1226 pKa = 10.06DD1227 pKa = 3.49PNGAFNGLSTGTTSADD1243 pKa = 3.13SFTYY1247 pKa = 10.81ALNGGSAASVSVTINGVTQVTGDD1270 pKa = 3.71SGDD1273 pKa = 3.59NNQAGTGSADD1283 pKa = 3.1NYY1285 pKa = 10.83NFSQGGDD1292 pKa = 3.59DD1293 pKa = 3.67TVSTGGGSDD1302 pKa = 4.0AIFFGAAFNQNDD1314 pKa = 4.33KK1315 pKa = 11.13VDD1317 pKa = 3.98GGAGNNDD1324 pKa = 3.25QLGLQGDD1331 pKa = 4.28YY1332 pKa = 10.99TGGNALTLGASNVTGVEE1349 pKa = 4.4VVALLAGFDD1358 pKa = 4.19YY1359 pKa = 11.32NVTTLDD1365 pKa = 3.94DD1366 pKa = 4.84LLTTGQTMTVFGGGLGAGDD1385 pKa = 4.25NLTFDD1390 pKa = 4.63GSAEE1394 pKa = 4.02TSGNFLVYY1402 pKa = 10.49GGLGTDD1408 pKa = 3.44VLTGGGGNDD1417 pKa = 3.46GFSFGAGRR1425 pKa = 11.84FNAATDD1431 pKa = 3.71VVNGGTGADD1440 pKa = 3.19QVALNGFTGTVSGTNFISVEE1460 pKa = 4.33TIVLATGSNTVTLADD1475 pKa = 3.72DD1476 pKa = 3.45WTLGGEE1482 pKa = 4.11SHH1484 pKa = 6.22TVFGSALSQGVTVDD1498 pKa = 4.4GSAEE1502 pKa = 4.0TDD1504 pKa = 3.1GRR1506 pKa = 11.84LALYY1510 pKa = 10.04GGSGNDD1516 pKa = 4.04LLTGGAGNDD1525 pKa = 4.21FIVGGAGADD1534 pKa = 3.86TIRR1537 pKa = 11.84GGLGADD1543 pKa = 4.38LLRR1546 pKa = 11.84GDD1548 pKa = 4.52GGADD1552 pKa = 3.24TFVLGSVAEE1561 pKa = 4.53STSTTHH1567 pKa = 6.63DD1568 pKa = 3.34TLIGFDD1574 pKa = 3.58VSADD1578 pKa = 3.84KK1579 pKa = 10.76IDD1581 pKa = 4.03LSTTITAIDD1590 pKa = 3.39AAITVGTLSNGSFDD1604 pKa = 4.67SDD1606 pKa = 3.74LTTALAGLGANHH1618 pKa = 8.01AITFTADD1625 pKa = 2.8QGDD1628 pKa = 3.59LAGRR1632 pKa = 11.84IFLVADD1638 pKa = 3.46TNGIAGYY1645 pKa = 9.18QAGQDD1650 pKa = 3.28AVFEE1654 pKa = 5.21LINPVQPITDD1664 pKa = 3.77PTPFII1669 pKa = 4.85

Molecular weight: 168.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R7IKR1|A0A2R7IKR1_9SPHN RND transporter (Fragment) OS=Sphingomonas sp. HMWF008 OX=2056845 GN=DBR17_18205 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.58GFRR19 pKa = 11.84SRR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.97KK41 pKa = 10.66LSAA44 pKa = 4.03

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3537

0

3537

974285

12

3638

275.5

29.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.677 ± 0.063

0.73 ± 0.012

5.942 ± 0.032

4.84 ± 0.041

3.618 ± 0.026

8.857 ± 0.044

1.903 ± 0.023

5.132 ± 0.025

2.956 ± 0.036

9.825 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.272 ± 0.023

2.566 ± 0.033

5.36 ± 0.035

3.035 ± 0.023

7.096 ± 0.041

5.241 ± 0.033

5.826 ± 0.039

7.428 ± 0.037

1.41 ± 0.018

2.276 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski