Micromonospora deserti
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5968 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W2D764|A0A2W2D764_9ACTN Condensation protein OS=Micromonospora deserti OX=2070366 GN=C1I99_17105 PE=4 SV=1
MM1 pKa = 7.24 QLGALTALALALSGCNLTSDD21 pKa = 5.66 DD22 pKa = 5.64 DD23 pKa = 6.32 DD24 pKa = 5.69 DD25 pKa = 5.63 DD26 pKa = 4.71 CALGPVGGGGTVALALRR43 pKa = 11.84 APEE46 pKa = 4.17 PAAPTAGEE54 pKa = 3.94 PAAAAVPEE62 pKa = 4.1 RR63 pKa = 11.84 GGFGTHH69 pKa = 6.99 LAACGGG75 pKa = 3.7
Molecular weight: 7.02 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.759
IPC2_protein 3.872
IPC_protein 3.745
Toseland 3.554
ProMoST 3.961
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.592
Grimsley 3.478
Solomon 3.732
Lehninger 3.681
Nozaki 3.91
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.745
Sillero 3.884
Patrickios 1.901
IPC_peptide 3.719
IPC2_peptide 3.846
IPC2.peptide.svr19 3.801
Protein with the highest isoelectric point:
>tr|A0A2W2DB67|A0A2W2DB67_9ACTN DUF2267 domain-containing protein OS=Micromonospora deserti OX=2070366 GN=C1I99_02385 PE=4 SV=1
MM1 pKa = 7.28 GSVVKK6 pKa = 10.49 KK7 pKa = 9.45 RR8 pKa = 11.84 RR9 pKa = 11.84 KK10 pKa = 9.48 RR11 pKa = 11.84 MAKK14 pKa = 9.41 KK15 pKa = 9.87 KK16 pKa = 9.7 HH17 pKa = 5.84 RR18 pKa = 11.84 KK19 pKa = 8.7 LLRR22 pKa = 11.84 KK23 pKa = 7.84 TRR25 pKa = 11.84 VQRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 LGKK33 pKa = 10.04
Molecular weight: 4.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.507
IPC2_protein 11.184
IPC_protein 12.749
Toseland 12.925
ProMoST 13.408
Dawson 12.925
Bjellqvist 12.91
Wikipedia 13.393
Rodwell 12.735
Grimsley 12.954
Solomon 13.408
Lehninger 13.305
Nozaki 12.91
DTASelect 12.91
Thurlkill 12.91
EMBOSS 13.408
Sillero 12.91
Patrickios 12.457
IPC_peptide 13.408
IPC2_peptide 12.398
IPC2.peptide.svr19 9.111
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5968
0
5968
1882724
22
3718
315.5
33.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.136 ± 0.049
0.754 ± 0.01
5.963 ± 0.026
5.008 ± 0.03
2.679 ± 0.019
9.513 ± 0.032
2.143 ± 0.017
3.164 ± 0.022
1.618 ± 0.023
10.449 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.597 ± 0.012
1.842 ± 0.02
6.371 ± 0.028
2.683 ± 0.017
8.556 ± 0.036
4.578 ± 0.024
6.073 ± 0.03
9.113 ± 0.035
1.629 ± 0.014
2.131 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here