Amino acid dipepetide frequency for Gordonia phage Ailee

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.903AlaAla: 15.903 ± 1.0
0.86AlaCys: 0.86 ± 0.232
8.059AlaAsp: 8.059 ± 0.709
9.187AlaGlu: 9.187 ± 1.004
2.901AlaPhe: 2.901 ± 0.518
10.906AlaGly: 10.906 ± 0.889
1.934AlaHis: 1.934 ± 0.291
6.769AlaIle: 6.769 ± 0.971
3.707AlaLys: 3.707 ± 0.543
9.08AlaLeu: 9.08 ± 0.808
2.31AlaMet: 2.31 ± 0.425
2.847AlaAsn: 2.847 ± 0.441
6.769AlaPro: 6.769 ± 0.688
4.406AlaGln: 4.406 ± 0.485
9.133AlaArg: 9.133 ± 0.835
5.587AlaSer: 5.587 ± 0.616
7.522AlaThr: 7.522 ± 0.757
8.274AlaVal: 8.274 ± 0.682
2.525AlaTrp: 2.525 ± 0.339
2.203AlaTyr: 2.203 ± 0.336
0.0AlaXaa: 0.0 ± 0.0
Cys
1.182CysAla: 1.182 ± 0.3
0.0CysCys: 0.0 ± 0.0
0.645CysAsp: 0.645 ± 0.207
0.484CysGlu: 0.484 ± 0.138
0.107CysPhe: 0.107 ± 0.069
1.343CysGly: 1.343 ± 0.326
0.591CysHis: 0.591 ± 0.186
0.0CysIle: 0.0 ± 0.0
0.215CysLys: 0.215 ± 0.121
0.215CysLeu: 0.215 ± 0.086
0.107CysMet: 0.107 ± 0.087
0.161CysAsn: 0.161 ± 0.085
0.967CysPro: 0.967 ± 0.207
0.269CysGln: 0.269 ± 0.104
0.591CysArg: 0.591 ± 0.169
0.43CysSer: 0.43 ± 0.146
0.645CysThr: 0.645 ± 0.216
0.484CysVal: 0.484 ± 0.177
0.107CysTrp: 0.107 ± 0.091
0.054CysTyr: 0.054 ± 0.057
0.0CysXaa: 0.0 ± 0.0
Asp
8.65AspAla: 8.65 ± 0.522
0.537AspCys: 0.537 ± 0.205
7.253AspAsp: 7.253 ± 0.971
5.749AspGlu: 5.749 ± 0.805
1.236AspPhe: 1.236 ± 0.307
6.984AspGly: 6.984 ± 0.714
1.988AspHis: 1.988 ± 0.377
1.666AspIle: 1.666 ± 0.369
1.343AspLys: 1.343 ± 0.276
5.265AspLeu: 5.265 ± 0.632
1.666AspMet: 1.666 ± 0.284
2.203AspAsn: 2.203 ± 0.353
7.629AspPro: 7.629 ± 0.777
2.149AspGln: 2.149 ± 0.475
5.426AspArg: 5.426 ± 0.706
2.095AspSer: 2.095 ± 0.281
3.868AspThr: 3.868 ± 0.524
6.555AspVal: 6.555 ± 0.701
1.666AspTrp: 1.666 ± 0.356
1.558AspTyr: 1.558 ± 0.338
0.0AspXaa: 0.0 ± 0.0
Glu
5.749GluAla: 5.749 ± 0.483
0.645GluCys: 0.645 ± 0.206
2.794GluAsp: 2.794 ± 0.43
1.827GluGlu: 1.827 ± 0.26
1.988GluPhe: 1.988 ± 0.389
4.029GluGly: 4.029 ± 0.552
2.31GluHis: 2.31 ± 0.43
2.525GluIle: 2.525 ± 0.326
1.236GluLys: 1.236 ± 0.344
5.319GluLeu: 5.319 ± 0.755
1.128GluMet: 1.128 ± 0.245
1.504GluAsn: 1.504 ± 0.239
3.277GluPro: 3.277 ± 0.647
4.137GluGln: 4.137 ± 0.569
4.513GluArg: 4.513 ± 0.563
2.686GluSer: 2.686 ± 0.422
3.009GluThr: 3.009 ± 0.472
4.728GluVal: 4.728 ± 0.541
1.558GluTrp: 1.558 ± 0.318
1.558GluTyr: 1.558 ± 0.293
0.0GluXaa: 0.0 ± 0.0
Phe
3.116PheAla: 3.116 ± 0.41
0.269PheCys: 0.269 ± 0.149
3.116PheAsp: 3.116 ± 0.427
1.827PheGlu: 1.827 ± 0.391
0.752PhePhe: 0.752 ± 0.239
2.686PheGly: 2.686 ± 0.328
0.269PheHis: 0.269 ± 0.116
1.397PheIle: 1.397 ± 0.281
0.752PheLys: 0.752 ± 0.151
1.397PheLeu: 1.397 ± 0.299
0.484PheMet: 0.484 ± 0.141
0.645PheAsn: 0.645 ± 0.202
1.021PhePro: 1.021 ± 0.242
0.752PheGln: 0.752 ± 0.248
0.967PheArg: 0.967 ± 0.244
0.967PheSer: 0.967 ± 0.261
1.666PheThr: 1.666 ± 0.273
2.364PheVal: 2.364 ± 0.288
0.43PheTrp: 0.43 ± 0.142
0.161PheTyr: 0.161 ± 0.085
0.0PheXaa: 0.0 ± 0.0
Gly
9.08GlyAla: 9.08 ± 0.788
0.591GlyCys: 0.591 ± 0.203
6.877GlyAsp: 6.877 ± 0.822
3.922GlyGlu: 3.922 ± 0.498
2.364GlyPhe: 2.364 ± 0.386
8.489GlyGly: 8.489 ± 1.079
2.364GlyHis: 2.364 ± 0.483
4.298GlyIle: 4.298 ± 0.499
3.277GlyLys: 3.277 ± 0.493
5.373GlyLeu: 5.373 ± 0.754
1.88GlyMet: 1.88 ± 0.277
2.686GlyAsn: 2.686 ± 0.415
3.976GlyPro: 3.976 ± 0.503
3.277GlyGln: 3.277 ± 0.426
5.91GlyArg: 5.91 ± 0.571
4.997GlySer: 4.997 ± 0.536
5.211GlyThr: 5.211 ± 0.77
7.629GlyVal: 7.629 ± 0.548
1.397GlyTrp: 1.397 ± 0.299
2.042GlyTyr: 2.042 ± 0.338
0.0GlyXaa: 0.0 ± 0.0
His
1.773HisAla: 1.773 ± 0.355
0.215HisCys: 0.215 ± 0.116
2.203HisAsp: 2.203 ± 0.412
1.558HisGlu: 1.558 ± 0.284
0.484HisPhe: 0.484 ± 0.159
1.128HisGly: 1.128 ± 0.191
0.537HisHis: 0.537 ± 0.228
0.806HisIle: 0.806 ± 0.279
0.43HisLys: 0.43 ± 0.142
1.827HisLeu: 1.827 ± 0.341
0.537HisMet: 0.537 ± 0.175
0.484HisAsn: 0.484 ± 0.151
1.504HisPro: 1.504 ± 0.298
1.128HisGln: 1.128 ± 0.227
1.934HisArg: 1.934 ± 0.323
0.86HisSer: 0.86 ± 0.223
2.095HisThr: 2.095 ± 0.397
1.988HisVal: 1.988 ± 0.379
0.322HisTrp: 0.322 ± 0.122
0.591HisTyr: 0.591 ± 0.177
0.0HisXaa: 0.0 ± 0.0
Ile
5.48IleAla: 5.48 ± 0.515
0.161IleCys: 0.161 ± 0.096
4.083IleAsp: 4.083 ± 0.455
3.707IleGlu: 3.707 ± 0.506
0.591IlePhe: 0.591 ± 0.21
5.319IleGly: 5.319 ± 0.937
0.645IleHis: 0.645 ± 0.173
2.579IleIle: 2.579 ± 0.391
1.075IleLys: 1.075 ± 0.402
2.471IleLeu: 2.471 ± 0.346
0.752IleMet: 0.752 ± 0.218
0.806IleAsn: 0.806 ± 0.195
2.633IlePro: 2.633 ± 0.312
1.343IleGln: 1.343 ± 0.296
2.955IleArg: 2.955 ± 0.387
2.042IleSer: 2.042 ± 0.366
3.331IleThr: 3.331 ± 0.445
3.492IleVal: 3.492 ± 0.324
0.645IleTrp: 0.645 ± 0.165
1.128IleTyr: 1.128 ± 0.214
0.0IleXaa: 0.0 ± 0.0
Lys
2.847LysAla: 2.847 ± 0.415
0.161LysCys: 0.161 ± 0.096
0.967LysAsp: 0.967 ± 0.232
0.537LysGlu: 0.537 ± 0.143
0.913LysPhe: 0.913 ± 0.254
1.558LysGly: 1.558 ± 0.328
0.86LysHis: 0.86 ± 0.219
1.397LysIle: 1.397 ± 0.265
0.537LysLys: 0.537 ± 0.187
2.364LysLeu: 2.364 ± 0.353
0.43LysMet: 0.43 ± 0.135
1.021LysAsn: 1.021 ± 0.276
2.149LysPro: 2.149 ± 0.425
0.967LysGln: 0.967 ± 0.211
1.934LysArg: 1.934 ± 0.371
1.612LysSer: 1.612 ± 0.346
1.988LysThr: 1.988 ± 0.34
2.256LysVal: 2.256 ± 0.374
0.269LysTrp: 0.269 ± 0.121
0.806LysTyr: 0.806 ± 0.214
0.0LysXaa: 0.0 ± 0.0
Leu
9.241LeuAla: 9.241 ± 0.852
0.537LeuCys: 0.537 ± 0.163
5.749LeuAsp: 5.749 ± 0.63
2.364LeuGlu: 2.364 ± 0.369
2.149LeuPhe: 2.149 ± 0.286
6.447LeuGly: 6.447 ± 1.025
1.773LeuHis: 1.773 ± 0.343
3.385LeuIle: 3.385 ± 0.389
0.913LeuLys: 0.913 ± 0.146
5.802LeuLeu: 5.802 ± 0.626
1.343LeuMet: 1.343 ± 0.263
2.31LeuAsn: 2.31 ± 0.292
4.889LeuPro: 4.889 ± 0.559
3.009LeuGln: 3.009 ± 0.473
6.017LeuArg: 6.017 ± 0.458
3.653LeuSer: 3.653 ± 0.469
5.48LeuThr: 5.48 ± 0.472
6.34LeuVal: 6.34 ± 0.474
2.095LeuTrp: 2.095 ± 0.301
1.397LeuTyr: 1.397 ± 0.273
0.0LeuXaa: 0.0 ± 0.0
Met
1.88MetAla: 1.88 ± 0.3
0.107MetCys: 0.107 ± 0.066
0.913MetAsp: 0.913 ± 0.186
0.698MetGlu: 0.698 ± 0.212
0.322MetPhe: 0.322 ± 0.151
1.236MetGly: 1.236 ± 0.235
0.269MetHis: 0.269 ± 0.119
0.537MetIle: 0.537 ± 0.151
0.645MetLys: 0.645 ± 0.216
2.042MetLeu: 2.042 ± 0.305
0.376MetMet: 0.376 ± 0.135
0.537MetAsn: 0.537 ± 0.21
2.095MetPro: 2.095 ± 0.311
0.913MetGln: 0.913 ± 0.286
1.397MetArg: 1.397 ± 0.282
1.451MetSer: 1.451 ± 0.298
3.224MetThr: 3.224 ± 0.359
1.343MetVal: 1.343 ± 0.3
0.43MetTrp: 0.43 ± 0.157
0.215MetTyr: 0.215 ± 0.085
0.0MetXaa: 0.0 ± 0.0
Asn
4.029AsnAla: 4.029 ± 0.54
0.322AsnCys: 0.322 ± 0.127
1.827AsnAsp: 1.827 ± 0.359
1.504AsnGlu: 1.504 ± 0.263
0.591AsnPhe: 0.591 ± 0.181
2.525AsnGly: 2.525 ± 0.31
0.537AsnHis: 0.537 ± 0.155
0.698AsnIle: 0.698 ± 0.181
0.376AsnLys: 0.376 ± 0.147
2.31AsnLeu: 2.31 ± 0.485
0.645AsnMet: 0.645 ± 0.175
0.913AsnAsn: 0.913 ± 0.231
2.633AsnPro: 2.633 ± 0.442
0.86AsnGln: 0.86 ± 0.202
1.988AsnArg: 1.988 ± 0.288
0.967AsnSer: 0.967 ± 0.226
1.504AsnThr: 1.504 ± 0.249
1.504AsnVal: 1.504 ± 0.231
0.752AsnTrp: 0.752 ± 0.217
0.698AsnTyr: 0.698 ± 0.203
0.0AsnXaa: 0.0 ± 0.0
Pro
9.187ProAla: 9.187 ± 0.724
0.537ProCys: 0.537 ± 0.258
5.91ProAsp: 5.91 ± 0.889
3.707ProGlu: 3.707 ± 0.608
1.719ProPhe: 1.719 ± 0.319
4.728ProGly: 4.728 ± 0.469
0.967ProHis: 0.967 ± 0.266
3.224ProIle: 3.224 ± 0.436
1.451ProLys: 1.451 ± 0.302
4.137ProLeu: 4.137 ± 0.528
1.397ProMet: 1.397 ± 0.223
1.988ProAsn: 1.988 ± 0.338
4.674ProPro: 4.674 ± 0.803
2.256ProGln: 2.256 ± 0.321
3.009ProArg: 3.009 ± 0.422
3.707ProSer: 3.707 ± 0.419
4.352ProThr: 4.352 ± 0.55
5.265ProVal: 5.265 ± 0.559
1.128ProTrp: 1.128 ± 0.225
1.289ProTyr: 1.289 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
5.319GlnAla: 5.319 ± 0.492
0.43GlnCys: 0.43 ± 0.147
1.236GlnAsp: 1.236 ± 0.283
0.967GlnGlu: 0.967 ± 0.252
1.236GlnPhe: 1.236 ± 0.23
1.988GlnGly: 1.988 ± 0.328
1.075GlnHis: 1.075 ± 0.232
1.988GlnIle: 1.988 ± 0.331
0.967GlnLys: 0.967 ± 0.213
3.546GlnLeu: 3.546 ± 0.48
1.021GlnMet: 1.021 ± 0.24
0.645GlnAsn: 0.645 ± 0.134
2.794GlnPro: 2.794 ± 0.423
3.224GlnGln: 3.224 ± 0.528
3.976GlnArg: 3.976 ± 0.463
1.343GlnSer: 1.343 ± 0.285
2.042GlnThr: 2.042 ± 0.294
2.579GlnVal: 2.579 ± 0.392
1.021GlnTrp: 1.021 ± 0.186
0.752GlnTyr: 0.752 ± 0.154
0.0GlnXaa: 0.0 ± 0.0
Arg
10.047ArgAla: 10.047 ± 0.802
0.645ArgCys: 0.645 ± 0.182
4.782ArgAsp: 4.782 ± 0.64
3.868ArgGlu: 3.868 ± 0.44
1.827ArgPhe: 1.827 ± 0.342
4.352ArgGly: 4.352 ± 0.516
1.558ArgHis: 1.558 ± 0.336
3.546ArgIle: 3.546 ± 0.449
2.418ArgLys: 2.418 ± 0.439
5.158ArgLeu: 5.158 ± 0.558
1.827ArgMet: 1.827 ± 0.279
2.095ArgAsn: 2.095 ± 0.321
3.868ArgPro: 3.868 ± 0.63
2.418ArgGln: 2.418 ± 0.348
8.059ArgArg: 8.059 ± 0.939
3.062ArgSer: 3.062 ± 0.389
4.083ArgThr: 4.083 ± 0.497
5.802ArgVal: 5.802 ± 0.779
1.666ArgTrp: 1.666 ± 0.327
1.988ArgTyr: 1.988 ± 0.401
0.0ArgXaa: 0.0 ± 0.0
Ser
4.352SerAla: 4.352 ± 0.558
0.484SerCys: 0.484 ± 0.175
3.546SerAsp: 3.546 ± 0.494
2.633SerGlu: 2.633 ± 0.431
0.967SerPhe: 0.967 ± 0.234
5.749SerGly: 5.749 ± 0.675
0.86SerHis: 0.86 ± 0.19
2.149SerIle: 2.149 ± 0.395
1.289SerLys: 1.289 ± 0.244
3.331SerLeu: 3.331 ± 0.364
0.967SerMet: 0.967 ± 0.253
1.021SerAsn: 1.021 ± 0.215
2.901SerPro: 2.901 ± 0.352
1.128SerGln: 1.128 ± 0.314
3.546SerArg: 3.546 ± 0.491
2.256SerSer: 2.256 ± 0.515
4.137SerThr: 4.137 ± 0.523
3.707SerVal: 3.707 ± 0.443
0.913SerTrp: 0.913 ± 0.213
0.913SerTyr: 0.913 ± 0.207
0.0SerXaa: 0.0 ± 0.0
Thr
9.187ThrAla: 9.187 ± 0.788
0.86ThrCys: 0.86 ± 0.222
5.158ThrAsp: 5.158 ± 0.647
3.438ThrGlu: 3.438 ± 0.437
1.451ThrPhe: 1.451 ± 0.261
6.125ThrGly: 6.125 ± 0.649
1.343ThrHis: 1.343 ± 0.327
3.062ThrIle: 3.062 ± 0.482
1.719ThrLys: 1.719 ± 0.293
5.319ThrLeu: 5.319 ± 0.46
1.236ThrMet: 1.236 ± 0.25
1.719ThrAsn: 1.719 ± 0.276
4.943ThrPro: 4.943 ± 0.451
1.451ThrGln: 1.451 ± 0.27
4.029ThrArg: 4.029 ± 0.484
3.116ThrSer: 3.116 ± 0.409
4.029ThrThr: 4.029 ± 0.492
5.265ThrVal: 5.265 ± 0.682
1.236ThrTrp: 1.236 ± 0.257
1.558ThrTyr: 1.558 ± 0.28
0.0ThrXaa: 0.0 ± 0.0
Val
8.704ValAla: 8.704 ± 0.785
0.698ValCys: 0.698 ± 0.243
7.36ValAsp: 7.36 ± 0.707
5.856ValGlu: 5.856 ± 0.625
2.149ValPhe: 2.149 ± 0.294
6.984ValGly: 6.984 ± 0.679
1.719ValHis: 1.719 ± 0.286
3.546ValIle: 3.546 ± 0.454
1.934ValLys: 1.934 ± 0.297
6.232ValLeu: 6.232 ± 0.541
1.075ValMet: 1.075 ± 0.203
2.633ValAsn: 2.633 ± 0.383
3.922ValPro: 3.922 ± 0.521
2.955ValGln: 2.955 ± 0.344
4.567ValArg: 4.567 ± 0.467
3.815ValSer: 3.815 ± 0.515
5.587ValThr: 5.587 ± 0.599
6.608ValVal: 6.608 ± 0.708
2.095ValTrp: 2.095 ± 0.278
1.988ValTyr: 1.988 ± 0.352
0.0ValXaa: 0.0 ± 0.0
Trp
2.149TrpAla: 2.149 ± 0.386
0.269TrpCys: 0.269 ± 0.113
1.182TrpAsp: 1.182 ± 0.242
1.021TrpGlu: 1.021 ± 0.263
0.698TrpPhe: 0.698 ± 0.2
1.289TrpGly: 1.289 ± 0.237
0.322TrpHis: 0.322 ± 0.125
0.86TrpIle: 0.86 ± 0.276
0.806TrpLys: 0.806 ± 0.202
2.203TrpLeu: 2.203 ± 0.312
0.86TrpMet: 0.86 ± 0.211
0.752TrpAsn: 0.752 ± 0.294
0.967TrpPro: 0.967 ± 0.206
0.86TrpGln: 0.86 ± 0.187
1.451TrpArg: 1.451 ± 0.304
1.289TrpSer: 1.289 ± 0.239
1.182TrpThr: 1.182 ± 0.232
2.042TrpVal: 2.042 ± 0.294
0.322TrpTrp: 0.322 ± 0.195
0.43TrpTyr: 0.43 ± 0.142
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.009TyrAla: 3.009 ± 0.476
0.269TyrCys: 0.269 ± 0.125
1.827TyrAsp: 1.827 ± 0.344
1.666TyrGlu: 1.666 ± 0.299
0.591TyrPhe: 0.591 ± 0.141
1.773TyrGly: 1.773 ± 0.248
0.484TyrHis: 0.484 ± 0.142
0.806TyrIle: 0.806 ± 0.192
0.484TyrLys: 0.484 ± 0.159
1.504TyrLeu: 1.504 ± 0.253
0.537TyrMet: 0.537 ± 0.161
0.215TyrAsn: 0.215 ± 0.096
1.021TyrPro: 1.021 ± 0.246
0.645TyrGln: 0.645 ± 0.185
1.666TyrArg: 1.666 ± 0.302
0.967TyrSer: 0.967 ± 0.193
1.289TyrThr: 1.289 ± 0.302
2.203TyrVal: 2.203 ± 0.324
0.376TyrTrp: 0.376 ± 0.14
0.269TyrTyr: 0.269 ± 0.113
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (18614 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski