Streptococcus satellite phage Javan218

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D5ZL10|A0A4D5ZL10_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan218 OX=2558575 GN=JavanS218_0016 PE=4 SV=1
MM1 pKa = 7.59ASSFYY6 pKa = 10.8DD7 pKa = 3.7YY8 pKa = 11.58DD9 pKa = 4.25DD10 pKa = 5.27LLVDD14 pKa = 3.77YY15 pKa = 10.45EE16 pKa = 4.57GLCGEE21 pKa = 4.89LEE23 pKa = 4.6IITDD27 pKa = 3.86VLEE30 pKa = 4.13LARR33 pKa = 11.84DD34 pKa = 3.75KK35 pKa = 11.28EE36 pKa = 4.23DD37 pKa = 3.28EE38 pKa = 4.08QTILLLHH45 pKa = 6.24TVTHH49 pKa = 6.33ALKK52 pKa = 10.57HH53 pKa = 5.63IVSEE57 pKa = 4.57HH58 pKa = 6.25KK59 pKa = 10.76NQASDD64 pKa = 3.74YY65 pKa = 8.36RR66 pKa = 11.84KK67 pKa = 10.31KK68 pKa = 10.69LATVPTIEE76 pKa = 4.03VV77 pKa = 3.25

Molecular weight: 8.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D5ZLS6|A0A4D5ZLS6_9VIRU Uncharacterized protein OS=Streptococcus satellite phage Javan218 OX=2558575 GN=JavanS218_0012 PE=4 SV=1
MM1 pKa = 7.62GPPPPSRR8 pKa = 11.84PRR10 pKa = 11.84FKK12 pKa = 10.06PSLYY16 pKa = 9.66IFSRR20 pKa = 11.84VIFIEE25 pKa = 3.91RR26 pKa = 11.84DD27 pKa = 2.75IMATIKK33 pKa = 10.32IKK35 pKa = 10.28CTVCGKK41 pKa = 10.09NFISHH46 pKa = 6.43TKK48 pKa = 8.07NACYY52 pKa = 10.03CSSEE56 pKa = 3.9CRR58 pKa = 11.84RR59 pKa = 11.84IGRR62 pKa = 11.84SRR64 pKa = 11.84ARR66 pKa = 11.84KK67 pKa = 7.87QWEE70 pKa = 3.84EE71 pKa = 3.58KK72 pKa = 9.1TNYY75 pKa = 10.02RR76 pKa = 11.84EE77 pKa = 4.0LQRR80 pKa = 11.84NKK82 pKa = 9.5MKK84 pKa = 10.6LRR86 pKa = 11.84RR87 pKa = 11.84KK88 pKa = 9.7LEE90 pKa = 3.87KK91 pKa = 9.96QQPVTKK97 pKa = 10.04KK98 pKa = 9.79EE99 pKa = 3.87PVLKK103 pKa = 10.39VEE105 pKa = 4.06RR106 pKa = 11.84VIPKK110 pKa = 9.35KK111 pKa = 10.29RR112 pKa = 11.84SYY114 pKa = 11.23EE115 pKa = 3.77EE116 pKa = 4.97LIEE119 pKa = 3.87YY120 pKa = 8.76WEE122 pKa = 4.06NYY124 pKa = 8.89RR125 pKa = 11.84LNLLKK130 pKa = 10.65SEE132 pKa = 4.38SVYY135 pKa = 11.08KK136 pKa = 10.76NQTHH140 pKa = 5.09TVGGIDD146 pKa = 3.02IHH148 pKa = 7.92EE149 pKa = 4.3EE150 pKa = 3.71DD151 pKa = 3.81FAEE154 pKa = 4.25KK155 pKa = 10.31VVRR158 pKa = 11.84QLRR161 pKa = 11.84GG162 pKa = 2.99

Molecular weight: 19.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

3089

61

387

162.6

18.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.568 ± 0.535

1.133 ± 0.244

5.73 ± 0.578

8.579 ± 0.641

3.593 ± 0.381

4.273 ± 0.442

1.716 ± 0.327

6.928 ± 0.329

10.78 ± 0.649

9.291 ± 0.623

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.942 ± 0.199

6.831 ± 0.441

2.687 ± 0.422

4.241 ± 0.323

4.273 ± 0.572

6.086 ± 0.412

5.406 ± 0.504

4.921 ± 0.405

1.068 ± 0.142

4.953 ± 0.387
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski