Brassica rapa subsp. pekinensis (Chinese cabbage) (Brassica pekinensis)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae;

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 40809 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4F551|M4F551_BRARP Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=3 SV=1
MM1 pKa = 7.98RR2 pKa = 11.84IKK4 pKa = 10.95LSVNCDD10 pKa = 2.77RR11 pKa = 11.84CRR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 10.57AMEE18 pKa = 3.89VAVNAKK24 pKa = 9.99GVISVAIEE32 pKa = 4.11GEE34 pKa = 4.41SEE36 pKa = 4.16DD37 pKa = 3.87EE38 pKa = 4.12LVVVGDD44 pKa = 5.1GIDD47 pKa = 3.48AACLVDD53 pKa = 3.61TLRR56 pKa = 11.84KK57 pKa = 7.14QACYY61 pKa = 10.33AILEE65 pKa = 4.43TLEE68 pKa = 4.19EE69 pKa = 4.37VNPDD73 pKa = 3.31ILTPDD78 pKa = 4.06DD79 pKa = 4.51DD80 pKa = 4.23QVQEE84 pKa = 4.62EE85 pKa = 5.07DD86 pKa = 5.44DD87 pKa = 4.19DD88 pKa = 4.36TDD90 pKa = 3.52DD91 pKa = 4.49SKK93 pKa = 11.76NIEE96 pKa = 4.72AADD99 pKa = 4.33DD100 pKa = 4.93DD101 pKa = 4.53NGTDD105 pKa = 3.33QKK107 pKa = 11.37VPPHH111 pKa = 6.68CCLAEE116 pKa = 4.36CPTICYY122 pKa = 7.42EE123 pKa = 4.18QPQHH127 pKa = 6.1EE128 pKa = 4.45MSEE131 pKa = 4.49VIVYY135 pKa = 10.11DD136 pKa = 3.41SYY138 pKa = 12.17GPTTGCTIMM147 pKa = 5.35

Molecular weight: 16.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4D2S8|M4D2S8_BRARP Uncharacterized protein OS=Brassica rapa subsp. pekinensis OX=51351 PE=4 SV=1
MM1 pKa = 7.09RR2 pKa = 11.84TRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84QADD9 pKa = 3.57RR10 pKa = 11.84SNSPNVRR17 pKa = 11.84VGLHH21 pKa = 5.12VRR23 pKa = 11.84SNSLVRR29 pKa = 11.84RR30 pKa = 11.84VGRR33 pKa = 11.84LARR36 pKa = 11.84SNSPVRR42 pKa = 11.84RR43 pKa = 11.84IGWRR47 pKa = 11.84GRR49 pKa = 3.28

Molecular weight: 5.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

40801

8

40809

15960268

49

5408

391.1

43.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.57 ± 0.01

1.79 ± 0.005

5.377 ± 0.009

6.765 ± 0.015

4.17 ± 0.009

6.561 ± 0.012

2.295 ± 0.005

4.982 ± 0.008

6.243 ± 0.012

9.432 ± 0.014

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.47 ± 0.005

4.232 ± 0.009

4.964 ± 0.015

3.472 ± 0.009

5.515 ± 0.009

9.095 ± 0.015

5.175 ± 0.007

6.819 ± 0.01

1.251 ± 0.004

2.782 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski