Eimeria brunetti

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Eimeriidae; Eimeria

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6LUK7|U6LUK7_9EIME Uncharacterized protein OS=Eimeria brunetti OX=51314 GN=EBH_0053530 PE=4 SV=1
MM1 pKa = 7.65ASAGVDD7 pKa = 3.43TLQGDD12 pKa = 3.81SSGLFDD18 pKa = 6.11DD19 pKa = 5.75EE20 pKa = 5.34EE21 pKa = 4.86DD22 pKa = 3.49SCMQQDD28 pKa = 4.33EE29 pKa = 4.71PLQQQLQQQLQQQQQQQQQQKK50 pKa = 10.11HH51 pKa = 5.38EE52 pKa = 4.34GSLPSSFSSSSAAAAAPGLDD72 pKa = 4.06DD73 pKa = 4.78FSFPATAATAAAAAAAAAADD93 pKa = 4.14SMSTPLSMEE102 pKa = 4.11PFQRR106 pKa = 11.84EE107 pKa = 3.72QQQQQQQQQQDD118 pKa = 3.62EE119 pKa = 4.41QQQQQQQQQQQEE131 pKa = 4.4EE132 pKa = 4.79EE133 pKa = 4.25EE134 pKa = 4.24QQQQQQQQQQQQEE147 pKa = 4.4EE148 pKa = 4.59EE149 pKa = 4.24VDD151 pKa = 3.84SEE153 pKa = 5.16AVLLQCSNCPCHH165 pKa = 7.55LLVYY169 pKa = 9.26EE170 pKa = 4.02QLAARR175 pKa = 11.84GLIFNFDD182 pKa = 3.6ILHH185 pKa = 6.5LLVSAAAKK193 pKa = 8.99PLPLWLQQLQQQQQQQQQQQQQQGEE218 pKa = 4.51LLQPMPYY225 pKa = 8.34TWGFQEE231 pKa = 4.2PQQTSLNLSLLRR243 pKa = 11.84YY244 pKa = 7.87TLDD247 pKa = 3.57CMQGSWIRR255 pKa = 11.84VWGLIFGVNGG265 pKa = 3.37

Molecular weight: 29.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6LT17|U6LT17_9EIME Uncharacterized protein OS=Eimeria brunetti OX=51314 GN=EBH_0037550 PE=4 SV=1
MM1 pKa = 6.92HH2 pKa = 6.95QEE4 pKa = 4.35SGSSSGSSSSSSSGGSSASSSSQQQRR30 pKa = 11.84QQQQQRR36 pKa = 11.84QQQRR40 pKa = 11.84QQQRR44 pKa = 11.84QHH46 pKa = 5.93QRR48 pKa = 11.84QQQQRR53 pKa = 11.84RR54 pKa = 11.84QQQQRR59 pKa = 11.84QQQQRR64 pKa = 11.84QQQQRR69 pKa = 11.84QQQQRR74 pKa = 11.84QQQQQRR80 pKa = 11.84QRR82 pKa = 11.84QQQQRR87 pKa = 11.84QQQQQRR93 pKa = 11.84QRR95 pKa = 11.84QQQQRR100 pKa = 11.84QQQRR104 pKa = 11.84QQRR107 pKa = 11.84QRR109 pKa = 11.84QQQQRR114 pKa = 11.84QQQRR118 pKa = 11.84QQQRR122 pKa = 11.84QQRR125 pKa = 11.84QRR127 pKa = 11.84QQQQRR132 pKa = 11.84QQQQQQRR139 pKa = 11.84QQIFFSWSSRR149 pKa = 11.84LPQDD153 pKa = 4.17FFQGPWGPLL162 pKa = 3.15

Molecular weight: 20.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8711

0

8711

4764477

44

9680

546.9

58.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

16.821 ± 0.112

1.674 ± 0.015

3.346 ± 0.024

5.902 ± 0.038

2.184 ± 0.019

5.98 ± 0.035

1.956 ± 0.014

2.164 ± 0.022

3.528 ± 0.032

8.855 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.281 ± 0.011

2.099 ± 0.016

5.553 ± 0.029

13.303 ± 0.131

5.535 ± 0.026

9.404 ± 0.056

4.203 ± 0.023

4.061 ± 0.033

0.843 ± 0.008

1.283 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski