Tent-making bat hepatitis B virus

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Blubervirales; Hepadnaviridae; Orthohepadnavirus

Average proteome isoelectric point is 8.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U3M9Y4|U3M9Y4_9HEPA DNA-directed DNA polymerase OS=Tent-making bat hepatitis B virus OX=1508712 PE=3 SV=1
MM1 pKa = 7.73ALPAAGLLGPLAVLQVGYY19 pKa = 8.98FLWTKK24 pKa = 9.29IQEE27 pKa = 4.01IGKK30 pKa = 7.65TVDD33 pKa = 2.81WWWISLSSPGGPTGCGGQNSLSPTSNHH60 pKa = 5.9SPISCPVTCSGYY72 pKa = 10.33RR73 pKa = 11.84WMCLRR78 pKa = 11.84RR79 pKa = 11.84FIIYY83 pKa = 10.51LLFLLLVLTFLLVLLDD99 pKa = 3.42WWGLLPVCPFMPVAGEE115 pKa = 3.75RR116 pKa = 11.84TINCRR121 pKa = 11.84TCTTSATEE129 pKa = 4.11APWRR133 pKa = 11.84PLCCCIMNTGGNCTCTPIPSSWALGKK159 pKa = 9.43YY160 pKa = 9.42LWGLASHH167 pKa = 6.71HH168 pKa = 6.05FSWLNLLQQFALWFAGLSSTAWLLLIWMMWYY199 pKa = 9.14WGPGLFPILSPFIPAVLIFWYY220 pKa = 9.98LWAA223 pKa = 5.89

Molecular weight: 25.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U3M9Y5|U3M9Y5_9HEPA Protein X OS=Tent-making bat hepatitis B virus OX=1508712 GN=X PE=3 SV=1
MM1 pKa = 7.49AARR4 pKa = 11.84MLCHH8 pKa = 6.83YY9 pKa = 10.55DD10 pKa = 3.21PTRR13 pKa = 11.84DD14 pKa = 3.53ILHH17 pKa = 6.57LRR19 pKa = 11.84PLRR22 pKa = 11.84GQSSGPALAGTPAAQAASPLPPVSPDD48 pKa = 3.04HH49 pKa = 6.94RR50 pKa = 11.84PDD52 pKa = 2.94ISLRR56 pKa = 11.84ALPACATSDD65 pKa = 3.61AGPCVLRR72 pKa = 11.84FTSADD77 pKa = 3.37LRR79 pKa = 11.84RR80 pKa = 11.84LATPVNICHH89 pKa = 6.44SLTRR93 pKa = 11.84RR94 pKa = 11.84ARR96 pKa = 11.84GHH98 pKa = 6.66PWTLNNMDD106 pKa = 3.1SWTWLMINRR115 pKa = 11.84EE116 pKa = 4.0VGSRR120 pKa = 11.84IEE122 pKa = 4.04YY123 pKa = 9.71VLVAGGCRR131 pKa = 11.84HH132 pKa = 6.41KK133 pKa = 11.1LQQ135 pKa = 4.13

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1373

135

827

343.3

38.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.919 ± 0.952

2.84 ± 0.771

3.496 ± 0.737

2.549 ± 0.626

4.37 ± 0.816

6.628 ± 0.767

3.714 ± 0.625

4.151 ± 0.461

3.423 ± 1.063

12.964 ± 0.961

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.966 ± 0.322

2.695 ± 0.055

8.303 ± 0.293

3.496 ± 0.636

6.846 ± 1.528

8.521 ± 0.376

5.754 ± 0.371

4.88 ± 0.299

3.277 ± 1.274

3.205 ± 0.41
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski