Amino acid dipepetide frequency for Stichopus japonicus (Sea cucumber)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.355AlaAla: 4.355 ± 0.026	1.194AlaCys: 1.194 ± 0.012	3.208AlaAsp: 3.208 ± 0.019	3.865AlaGlu: 3.865 ± 0.023	2.316AlaPhe: 2.316 ± 0.015	3.489AlaGly: 3.489 ± 0.025	1.175AlaHis: 1.175 ± 0.011	3.218AlaIle: 3.218 ± 0.019	3.61AlaLys: 3.61 ± 0.023	5.182AlaLeu: 5.182 ± 0.024	1.483AlaMet: 1.483 ± 0.013	2.407AlaAsn: 2.407 ± 0.017	2.462AlaPro: 2.462 ± 0.018	2.045AlaGln: 2.045 ± 0.015	2.787AlaArg: 2.787 ± 0.019	4.808AlaSer: 4.808 ± 0.023	3.692AlaThr: 3.692 ± 0.023	4.347AlaVal: 4.347 ± 0.024	0.618AlaTrp: 0.618 ± 0.009	1.594AlaTyr: 1.594 ± 0.013	0.001AlaXaa: 0.001 ± 0.0
Cys	1.089CysAla: 1.089 ± 0.013	0.547CysCys: 0.547 ± 0.016	1.334CysAsp: 1.334 ± 0.022	1.34CysGlu: 1.34 ± 0.019	0.893CysPhe: 0.893 ± 0.01	1.405CysGly: 1.405 ± 0.016	0.619CysHis: 0.619 ± 0.009	1.23CysIle: 1.23 ± 0.012	1.221CysLys: 1.221 ± 0.012	2.075CysLeu: 2.075 ± 0.018	0.449CysMet: 0.449 ± 0.007	1.097CysAsn: 1.097 ± 0.013	1.308CysPro: 1.308 ± 0.031	1.098CysGln: 1.098 ± 0.015	1.19CysArg: 1.19 ± 0.012	1.997CysSer: 1.997 ± 0.021	1.325CysThr: 1.325 ± 0.015	1.335CysVal: 1.335 ± 0.016	0.244CysTrp: 0.244 ± 0.004	0.68CysTyr: 0.68 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	3.23AspAla: 3.23 ± 0.021	1.254AspCys: 1.254 ± 0.017	4.819AspAsp: 4.819 ± 0.112	4.532AspGlu: 4.532 ± 0.027	2.319AspPhe: 2.319 ± 0.014	4.262AspGly: 4.262 ± 0.033	1.384AspHis: 1.384 ± 0.018	3.655AspIle: 3.655 ± 0.023	3.151AspLys: 3.151 ± 0.019	5.038AspLeu: 5.038 ± 0.025	1.278AspMet: 1.278 ± 0.01	2.563AspAsn: 2.563 ± 0.022	2.572AspPro: 2.572 ± 0.018	2.178AspGln: 2.178 ± 0.016	2.733AspArg: 2.733 ± 0.017	4.353AspSer: 4.353 ± 0.022	3.018AspThr: 3.018 ± 0.021	4.213AspVal: 4.213 ± 0.023	0.715AspTrp: 0.715 ± 0.009	1.756AspTyr: 1.756 ± 0.014	0.0AspXaa: 0.0 ± 0.0
Glu	4.114GluAla: 4.114 ± 0.022	1.307GluCys: 1.307 ± 0.02	4.766GluAsp: 4.766 ± 0.029	7.098GluGlu: 7.098 ± 0.056	2.232GluPhe: 2.232 ± 0.014	4.114GluGly: 4.114 ± 0.031	1.338GluHis: 1.338 ± 0.011	3.655GluIle: 3.655 ± 0.023	4.931GluLys: 4.931 ± 0.039	5.489GluLeu: 5.489 ± 0.03	1.785GluMet: 1.785 ± 0.015	3.237GluAsn: 3.237 ± 0.019	2.379GluPro: 2.379 ± 0.018	2.637GluGln: 2.637 ± 0.021	3.951GluArg: 3.951 ± 0.034	4.709GluSer: 4.709 ± 0.025	4.009GluThr: 4.009 ± 0.022	4.492GluVal: 4.492 ± 0.024	0.755GluTrp: 0.755 ± 0.008	1.834GluTyr: 1.834 ± 0.014	0.001GluXaa: 0.001 ± 0.0
Phe	2.216PheAla: 2.216 ± 0.016	0.983PheCys: 0.983 ± 0.011	2.219PheAsp: 2.219 ± 0.016	2.263PheGlu: 2.263 ± 0.014	1.629PhePhe: 1.629 ± 0.013	2.455PheGly: 2.455 ± 0.015	1.068PheHis: 1.068 ± 0.01	2.231PheIle: 2.231 ± 0.016	2.071PheLys: 2.071 ± 0.016	3.711PheLeu: 3.711 ± 0.022	0.876PheMet: 0.876 ± 0.01	1.747PheAsn: 1.747 ± 0.014	1.684PhePro: 1.684 ± 0.012	1.769PheGln: 1.769 ± 0.013	1.927PheArg: 1.927 ± 0.013	3.207PheSer: 3.207 ± 0.018	2.416PheThr: 2.416 ± 0.017	2.626PheVal: 2.626 ± 0.014	0.52PheTrp: 0.52 ± 0.007	1.276PheTyr: 1.276 ± 0.011	0.0PheXaa: 0.0 ± 0.0
Gly	3.158GlyAla: 3.158 ± 0.019	1.256GlyCys: 1.256 ± 0.015	3.647GlyAsp: 3.647 ± 0.025	3.906GlyGlu: 3.906 ± 0.028	2.445GlyPhe: 2.445 ± 0.019	4.333GlyGly: 4.333 ± 0.043	1.601GlyHis: 1.601 ± 0.013	3.233GlyIle: 3.233 ± 0.019	3.704GlyLys: 3.704 ± 0.023	4.725GlyLeu: 4.725 ± 0.022	1.375GlyMet: 1.375 ± 0.013	3.023GlyAsn: 3.023 ± 0.02	2.381GlyPro: 2.381 ± 0.024	2.417GlyGln: 2.417 ± 0.02	3.345GlyArg: 3.345 ± 0.021	5.147GlySer: 5.147 ± 0.028	3.663GlyThr: 3.663 ± 0.027	3.778GlyVal: 3.778 ± 0.025	0.8GlyTrp: 0.8 ± 0.019	2.079GlyTyr: 2.079 ± 0.022	0.001GlyXaa: 0.001 ± 0.0
His	1.179HisAla: 1.179 ± 0.01	0.633HisCys: 0.633 ± 0.008	1.208HisAsp: 1.208 ± 0.018	1.36HisGlu: 1.36 ± 0.014	1.039HisPhe: 1.039 ± 0.01	1.471HisGly: 1.471 ± 0.012	0.916HisHis: 0.916 ± 0.018	1.367HisIle: 1.367 ± 0.012	1.277HisLys: 1.277 ± 0.011	2.487HisLeu: 2.487 ± 0.016	0.56HisMet: 0.56 ± 0.008	1.012HisAsn: 1.012 ± 0.01	1.365HisPro: 1.365 ± 0.013	1.211HisGln: 1.211 ± 0.012	1.471HisArg: 1.471 ± 0.016	1.972HisSer: 1.972 ± 0.016	1.281HisThr: 1.281 ± 0.013	1.482HisVal: 1.482 ± 0.012	0.319HisTrp: 0.319 ± 0.006	0.778HisTyr: 0.778 ± 0.008	0.001HisXaa: 0.001 ± 0.0
Ile	3.194IleAla: 3.194 ± 0.018	1.286IleCys: 1.286 ± 0.013	2.987IleAsp: 2.987 ± 0.016	3.119IleGlu: 3.119 ± 0.021	2.245IlePhe: 2.245 ± 0.019	2.916IleGly: 2.916 ± 0.018	1.442IleHis: 1.442 ± 0.013	3.077IleIle: 3.077 ± 0.026	2.999IleLys: 2.999 ± 0.017	5.11IleLeu: 5.11 ± 0.025	1.125IleMet: 1.125 ± 0.011	2.412IleAsn: 2.412 ± 0.016	3.004IlePro: 3.004 ± 0.018	2.342IleGln: 2.342 ± 0.015	2.685IleArg: 2.685 ± 0.019	4.487IleSer: 4.487 ± 0.021	3.415IleThr: 3.415 ± 0.019	3.318IleVal: 3.318 ± 0.021	0.61IleTrp: 0.61 ± 0.008	1.598IleTyr: 1.598 ± 0.014	0.001IleXaa: 0.001 ± 0.0
Lys	3.583LysAla: 3.583 ± 0.02	1.176LysCys: 1.176 ± 0.013	3.64LysAsp: 3.64 ± 0.022	5.111LysGlu: 5.111 ± 0.041	2.058LysPhe: 2.058 ± 0.016	3.298LysGly: 3.298 ± 0.021	1.436LysHis: 1.436 ± 0.012	3.034LysIle: 3.034 ± 0.019	4.907LysLys: 4.907 ± 0.04	5.553LysLeu: 5.553 ± 0.031	1.56LysMet: 1.56 ± 0.011	2.467LysAsn: 2.467 ± 0.015	2.782LysPro: 2.782 ± 0.02	2.705LysGln: 2.705 ± 0.022	3.759LysArg: 3.759 ± 0.026	4.412LysSer: 4.412 ± 0.025	3.576LysThr: 3.576 ± 0.02	3.905LysVal: 3.905 ± 0.02	0.7LysTrp: 0.7 ± 0.009	1.798LysTyr: 1.798 ± 0.013	0.001LysXaa: 0.001 ± 0.0
Leu	5.276LeuAla: 5.276 ± 0.026	1.956LeuCys: 1.956 ± 0.017	4.801LeuAsp: 4.801 ± 0.023	5.995LeuGlu: 5.995 ± 0.034	3.387LeuPhe: 3.387 ± 0.02	4.595LeuGly: 4.595 ± 0.022	2.339LeuHis: 2.339 ± 0.018	4.26LeuIle: 4.26 ± 0.022	5.823LeuLys: 5.823 ± 0.029	8.443LeuLeu: 8.443 ± 0.042	2.112LeuMet: 2.112 ± 0.015	3.804LeuAsn: 3.804 ± 0.019	4.829LeuPro: 4.829 ± 0.023	4.602LeuGln: 4.602 ± 0.027	4.88LeuArg: 4.88 ± 0.024	7.385LeuSer: 7.385 ± 0.033	5.386LeuThr: 5.386 ± 0.025	5.379LeuVal: 5.379 ± 0.025	0.991LeuTrp: 0.991 ± 0.011	2.579LeuTyr: 2.579 ± 0.017	0.001LeuXaa: 0.001 ± 0.0
Met	1.672MetAla: 1.672 ± 0.012	0.441MetCys: 0.441 ± 0.006	1.433MetAsp: 1.433 ± 0.019	1.842MetGlu: 1.842 ± 0.013	0.904MetPhe: 0.904 ± 0.009	1.198MetGly: 1.198 ± 0.011	0.47MetHis: 0.47 ± 0.007	1.126MetIle: 1.126 ± 0.011	1.759MetLys: 1.759 ± 0.014	1.942MetLeu: 1.942 ± 0.014	0.706MetMet: 0.706 ± 0.012	1.056MetAsn: 1.056 ± 0.01	1.005MetPro: 1.005 ± 0.01	0.958MetGln: 0.958 ± 0.01	1.163MetArg: 1.163 ± 0.011	1.806MetSer: 1.806 ± 0.014	1.438MetThr: 1.438 ± 0.012	1.534MetVal: 1.534 ± 0.013	0.249MetTrp: 0.249 ± 0.005	0.674MetTyr: 0.674 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.44AsnAla: 2.44 ± 0.019	1.098AsnCys: 1.098 ± 0.013	2.51AsnAsp: 2.51 ± 0.022	2.756AsnGlu: 2.756 ± 0.017	1.796AsnPhe: 1.796 ± 0.012	3.171AsnGly: 3.171 ± 0.025	1.11AsnHis: 1.11 ± 0.011	2.81AsnIle: 2.81 ± 0.017	2.487AsnLys: 2.487 ± 0.018	4.074AsnLeu: 4.074 ± 0.02	1.044AsnMet: 1.044 ± 0.009	2.233AsnAsn: 2.233 ± 0.017	2.209AsnPro: 2.209 ± 0.016	2.054AsnGln: 2.054 ± 0.017	2.312AsnArg: 2.312 ± 0.015	3.505AsnSer: 3.505 ± 0.019	2.443AsnThr: 2.443 ± 0.017	2.992AsnVal: 2.992 ± 0.02	0.524AsnTrp: 0.524 ± 0.007	1.412AsnTyr: 1.412 ± 0.014	0.001AsnXaa: 0.001 ± 0.0
Pro	2.866ProAla: 2.866 ± 0.02	0.972ProCys: 0.972 ± 0.02	2.743ProAsp: 2.743 ± 0.017	3.24ProGlu: 3.24 ± 0.022	1.854ProPhe: 1.854 ± 0.014	3.025ProGly: 3.025 ± 0.026	1.101ProHis: 1.101 ± 0.011	2.278ProIle: 2.278 ± 0.015	2.59ProLys: 2.59 ± 0.018	3.979ProLeu: 3.979 ± 0.021	0.998ProMet: 0.998 ± 0.012	2.048ProAsn: 2.048 ± 0.017	3.556ProPro: 3.556 ± 0.037	1.925ProGln: 1.925 ± 0.017	2.395ProArg: 2.395 ± 0.018	4.651ProSer: 4.651 ± 0.024	3.083ProThr: 3.083 ± 0.023	3.36ProVal: 3.36 ± 0.022	0.5ProTrp: 0.5 ± 0.007	1.405ProTyr: 1.405 ± 0.013	0.001ProXaa: 0.001 ± 0.0
Gln	2.454GlnAla: 2.454 ± 0.018	0.952GlnCys: 0.952 ± 0.013	2.195GlnAsp: 2.195 ± 0.016	3.21GlnGlu: 3.21 ± 0.021	1.513GlnPhe: 1.513 ± 0.011	2.364GlnGly: 2.364 ± 0.017	1.075GlnHis: 1.075 ± 0.01	2.087GlnIle: 2.087 ± 0.015	2.613GlnLys: 2.613 ± 0.018	4.026GlnLeu: 4.026 ± 0.024	1.07GlnMet: 1.07 ± 0.011	1.976GlnAsn: 1.976 ± 0.014	2.023GlnPro: 2.023 ± 0.017	2.52GlnGln: 2.52 ± 0.03	2.778GlnArg: 2.778 ± 0.019	3.279GlnSer: 3.279 ± 0.022	2.64GlnThr: 2.64 ± 0.02	2.555GlnVal: 2.555 ± 0.017	0.502GlnTrp: 0.502 ± 0.006	1.305GlnTyr: 1.305 ± 0.012	0.0GlnXaa: 0.0 ± 0.0
Arg	2.76ArgAla: 2.76 ± 0.019	1.198ArgCys: 1.198 ± 0.012	2.958ArgAsp: 2.958 ± 0.02	3.642ArgGlu: 3.642 ± 0.023	2.008ArgPhe: 2.008 ± 0.015	3.144ArgGly: 3.144 ± 0.025	1.402ArgHis: 1.402 ± 0.013	2.716ArgIle: 2.716 ± 0.016	3.943ArgLys: 3.943 ± 0.03	4.855ArgLeu: 4.855 ± 0.023	1.298ArgMet: 1.298 ± 0.011	2.616ArgAsn: 2.616 ± 0.015	2.5ArgPro: 2.5 ± 0.018	2.522ArgGln: 2.522 ± 0.019	4.151ArgArg: 4.151 ± 0.031	4.118ArgSer: 4.118 ± 0.024	2.908ArgThr: 2.908 ± 0.017	3.042ArgVal: 3.042 ± 0.019	0.717ArgTrp: 0.717 ± 0.008	1.674ArgTyr: 1.674 ± 0.014	0.0ArgXaa: 0.0 ± 0.0
Ser	4.447SerAla: 4.447 ± 0.024	1.872SerCys: 1.872 ± 0.019	4.836SerAsp: 4.836 ± 0.026	4.943SerGlu: 4.943 ± 0.025	3.304SerPhe: 3.304 ± 0.019	5.111SerGly: 5.111 ± 0.027	1.989SerHis: 1.989 ± 0.016	3.968SerIle: 3.968 ± 0.022	4.78SerLys: 4.78 ± 0.027	7.317SerLeu: 7.317 ± 0.032	1.748SerMet: 1.748 ± 0.013	3.678SerAsn: 3.678 ± 0.024	4.311SerPro: 4.311 ± 0.027	3.511SerGln: 3.511 ± 0.02	4.247SerArg: 4.247 ± 0.027	9.096SerSer: 9.096 ± 0.056	5.208SerThr: 5.208 ± 0.034	5.173SerVal: 5.173 ± 0.024	0.957SerTrp: 0.957 ± 0.011	2.347SerTyr: 2.347 ± 0.016	0.001SerXaa: 0.001 ± 0.0
Thr	3.752ThrAla: 3.752 ± 0.023	1.641ThrCys: 1.641 ± 0.027	3.572ThrAsp: 3.572 ± 0.029	4.034ThrGlu: 4.034 ± 0.026	2.486ThrPhe: 2.486 ± 0.016	3.804ThrGly: 3.804 ± 0.028	1.254ThrHis: 1.254 ± 0.012	3.302ThrIle: 3.302 ± 0.023	3.306ThrLys: 3.306 ± 0.018	5.267ThrLeu: 5.267 ± 0.025	1.326ThrMet: 1.326 ± 0.012	2.679ThrAsn: 2.679 ± 0.017	3.218ThrPro: 3.218 ± 0.025	2.129ThrGln: 2.129 ± 0.017	2.738ThrArg: 2.738 ± 0.015	5.482ThrSer: 5.482 ± 0.029	4.493ThrThr: 4.493 ± 0.068	4.37ThrVal: 4.37 ± 0.027	0.696ThrTrp: 0.696 ± 0.008	1.719ThrTyr: 1.719 ± 0.014	0.001ThrXaa: 0.001 ± 0.0
Val	3.956ValAla: 3.956 ± 0.02	1.602ValCys: 1.602 ± 0.017	3.749ValAsp: 3.749 ± 0.02	4.174ValGlu: 4.174 ± 0.024	2.639ValPhe: 2.639 ± 0.017	3.453ValGly: 3.453 ± 0.021	1.503ValHis: 1.503 ± 0.014	3.779ValIle: 3.779 ± 0.021	3.843ValLys: 3.843 ± 0.021	5.661ValLeu: 5.661 ± 0.024	1.547ValMet: 1.547 ± 0.014	2.914ValAsn: 2.914 ± 0.018	3.12ValPro: 3.12 ± 0.019	2.564ValGln: 2.564 ± 0.015	3.171ValArg: 3.171 ± 0.017	5.194ValSer: 5.194 ± 0.022	4.782ValThr: 4.782 ± 0.037	4.491ValVal: 4.491 ± 0.025	0.743ValTrp: 0.743 ± 0.008	1.923ValTyr: 1.923 ± 0.015	0.0ValXaa: 0.0 ± 0.0
Trp	0.553TrpAla: 0.553 ± 0.008	0.266TrpCys: 0.266 ± 0.005	0.666TrpAsp: 0.666 ± 0.01	0.675TrpGlu: 0.675 ± 0.007	0.493TrpPhe: 0.493 ± 0.008	0.596TrpGly: 0.596 ± 0.01	0.269TrpHis: 0.269 ± 0.005	0.635TrpIle: 0.635 ± 0.008	0.853TrpLys: 0.853 ± 0.009	1.105TrpLeu: 1.105 ± 0.01	0.377TrpMet: 0.377 ± 0.016	0.599TrpAsn: 0.599 ± 0.008	0.471TrpPro: 0.471 ± 0.007	0.528TrpGln: 0.528 ± 0.008	0.732TrpArg: 0.732 ± 0.008	0.92TrpSer: 0.92 ± 0.01	0.791TrpThr: 0.791 ± 0.009	0.632TrpVal: 0.632 ± 0.008	0.193TrpTrp: 0.193 ± 0.005	0.408TrpTyr: 0.408 ± 0.006	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.562TyrAla: 1.562 ± 0.014	0.841TyrCys: 0.841 ± 0.019	1.73TyrAsp: 1.73 ± 0.014	1.729TyrGlu: 1.729 ± 0.013	1.331TyrPhe: 1.331 ± 0.011	1.813TyrGly: 1.813 ± 0.015	0.901TyrHis: 0.901 ± 0.01	1.714TyrIle: 1.714 ± 0.014	1.588TyrLys: 1.588 ± 0.013	2.783TyrLeu: 2.783 ± 0.018	0.658TyrMet: 0.658 ± 0.008	1.412TyrAsn: 1.412 ± 0.011	1.39TyrPro: 1.39 ± 0.014	1.425TyrGln: 1.425 ± 0.015	1.751TyrArg: 1.751 ± 0.015	2.317TyrSer: 2.317 ± 0.017	1.702TyrThr: 1.702 ± 0.015	1.783TyrVal: 1.783 ± 0.013	0.411TyrTrp: 0.411 ± 0.006	1.084TyrTyr: 1.084 ± 0.012	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 30032 proteins (11815549 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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