Pseudomonas pelagia

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4145 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2A4AWA0|A0A2A4AWA0_9PSED LysR family transcriptional regulator OS=Pseudomonas pelagia OX=553151 GN=CO192_07230 PE=3 SV=1
MM1 pKa = 7.29SACLDD6 pKa = 3.62SDD8 pKa = 4.09GGSSSADD15 pKa = 2.98NDD17 pKa = 3.71AAPPPEE23 pKa = 4.07NTKK26 pKa = 10.65PNLILIIADD35 pKa = 3.59DD36 pKa = 3.98VGVDD40 pKa = 3.42QLASFGFGGAEE51 pKa = 3.97PPATPTIDD59 pKa = 3.27SLAATGVSFNNTWSMPTCSTTRR81 pKa = 11.84AALLSGRR88 pKa = 11.84YY89 pKa = 8.63PPRR92 pKa = 11.84TNVNTAIVSTDD103 pKa = 3.62FANSQTSPFEE113 pKa = 3.91RR114 pKa = 11.84SLPTVLGEE122 pKa = 3.91QGYY125 pKa = 9.71VSAYY129 pKa = 8.53VGKK132 pKa = 9.96IHH134 pKa = 6.21VTGSDD139 pKa = 3.62VNPANHH145 pKa = 7.18PFGDD149 pKa = 3.7EE150 pKa = 4.32AIRR153 pKa = 11.84ALGFDD158 pKa = 3.62YY159 pKa = 10.68FAGYY163 pKa = 10.18FDD165 pKa = 4.6GGPRR169 pKa = 11.84PIDD172 pKa = 3.34VTAGLDD178 pKa = 3.68NLDD181 pKa = 4.14SSAAPYY187 pKa = 9.41TCGFVPLATDD197 pKa = 4.1HH198 pKa = 6.64PQGADD203 pKa = 2.86TGACYY208 pKa = 9.92QPSGACEE215 pKa = 4.04EE216 pKa = 4.29LTADD220 pKa = 4.06GSSVPGKK227 pKa = 9.36MCLEE231 pKa = 4.23RR232 pKa = 11.84GGLFDD237 pKa = 6.32PNQQCASTLPAYY249 pKa = 10.9LDD251 pKa = 3.65FTRR254 pKa = 11.84QNAYY258 pKa = 8.27YY259 pKa = 8.35TAEE262 pKa = 4.08VVISEE267 pKa = 4.38EE268 pKa = 4.16SGSQQLEE275 pKa = 3.84VDD277 pKa = 3.6NPATRR282 pKa = 11.84LYY284 pKa = 9.47RR285 pKa = 11.84TQLEE289 pKa = 4.72SNFAIDD295 pKa = 4.31WIQAQSDD302 pKa = 3.91EE303 pKa = 4.46QPWMLSLGYY312 pKa = 9.46SASHH316 pKa = 6.93APLQPTPASLTPEE329 pKa = 4.06TGALDD334 pKa = 4.24IGIACDD340 pKa = 3.31NGADD344 pKa = 3.67TRR346 pKa = 11.84KK347 pKa = 10.3LMNQNMEE354 pKa = 4.21AMDD357 pKa = 3.73TEE359 pKa = 4.06IRR361 pKa = 11.84RR362 pKa = 11.84VLLAAGVYY370 pKa = 9.68IEE372 pKa = 4.64NANGDD377 pKa = 4.18LEE379 pKa = 4.52YY380 pKa = 11.18NPASNTVVAFIGDD393 pKa = 3.61NGTYY397 pKa = 10.45GPVVKK402 pKa = 10.69DD403 pKa = 3.3PFLPARR409 pKa = 11.84SKK411 pKa = 9.79GTVYY415 pKa = 10.44QGGIWVPLIVTGATVEE431 pKa = 4.05QPGRR435 pKa = 11.84SVGAMVNSTDD445 pKa = 4.48LYY447 pKa = 11.22HH448 pKa = 7.49LFATLGGADD457 pKa = 3.85LSAPEE462 pKa = 3.97VADD465 pKa = 4.57RR466 pKa = 11.84LDD468 pKa = 4.15AQPMDD473 pKa = 4.38AYY475 pKa = 10.53LASAEE480 pKa = 4.66AIPIRR485 pKa = 11.84DD486 pKa = 3.79SNFSMSGRR494 pKa = 11.84NLASAIPQPCVLTDD508 pKa = 3.58VNICLQLFPQKK519 pKa = 10.36GLCISEE525 pKa = 3.96GGTWYY530 pKa = 10.78GDD532 pKa = 3.54DD533 pKa = 3.83GVVPGQSFTSCCAVNTYY550 pKa = 9.88RR551 pKa = 11.84VSEE554 pKa = 4.4GEE556 pKa = 4.02APFDD560 pKa = 3.93ILAEE564 pKa = 4.22TQRR567 pKa = 11.84TVRR570 pKa = 11.84DD571 pKa = 3.6DD572 pKa = 3.61RR573 pKa = 11.84FKK575 pKa = 11.22LLQLEE580 pKa = 4.71EE581 pKa = 4.79PNCDD585 pKa = 3.0AGGLTEE591 pKa = 4.79SFEE594 pKa = 4.28FYY596 pKa = 10.5EE597 pKa = 4.38INEE600 pKa = 4.42SGSEE604 pKa = 3.93LGLDD608 pKa = 3.57NLDD611 pKa = 4.04AQDD614 pKa = 4.19LLTRR618 pKa = 11.84SALTAEE624 pKa = 4.46QQQHH628 pKa = 5.78YY629 pKa = 7.97NTLLAEE635 pKa = 4.68LDD637 pKa = 4.19SIVDD641 pKa = 4.04SEE643 pKa = 4.58PTCPGDD649 pKa = 3.58GNLDD653 pKa = 3.53RR654 pKa = 11.84VINEE658 pKa = 3.83QDD660 pKa = 3.48LKK662 pKa = 10.61DD663 pKa = 3.34WSYY666 pKa = 11.45FSQLNGGRR674 pKa = 11.84SSWYY678 pKa = 10.17DD679 pKa = 3.41FNLDD683 pKa = 3.65GQTDD687 pKa = 3.4AADD690 pKa = 3.83RR691 pKa = 11.84QIIEE695 pKa = 4.73DD696 pKa = 3.97NLGLEE701 pKa = 4.68CLL703 pKa = 4.09

Molecular weight: 75.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2A4AYQ5|A0A2A4AYQ5_9PSED YkuD domain-containing protein OS=Pseudomonas pelagia OX=553151 GN=CO192_00945 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.46RR12 pKa = 11.84KK13 pKa = 9.37RR14 pKa = 11.84NHH16 pKa = 5.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATANGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.93GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4145

0

4145

1348114

23

5694

325.2

35.77

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.527 ± 0.047

1.015 ± 0.014

5.567 ± 0.032

6.021 ± 0.036

3.607 ± 0.021

7.778 ± 0.043

2.258 ± 0.023

5.143 ± 0.03

3.346 ± 0.033

11.48 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.02

3.11 ± 0.029

4.718 ± 0.028

4.649 ± 0.031

6.297 ± 0.038

5.96 ± 0.033

4.901 ± 0.026

7.033 ± 0.035

1.426 ± 0.016

2.525 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski