Amino acid dipepetide frequency for Methylobacterium gnaphalii

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.541AlaAla: 19.541 ± 0.207	1.175AlaCys: 1.175 ± 0.038	6.941AlaAsp: 6.941 ± 0.079	8.389AlaGlu: 8.389 ± 0.1	4.576AlaPhe: 4.576 ± 0.056	11.51AlaGly: 11.51 ± 0.113	2.313AlaHis: 2.313 ± 0.049	6.283AlaIle: 6.283 ± 0.071	4.18AlaLys: 4.18 ± 0.078	14.457AlaLeu: 14.457 ± 0.136	3.529AlaMet: 3.529 ± 0.05	2.72AlaAsn: 2.72 ± 0.053	6.371AlaPro: 6.371 ± 0.087	4.155AlaGln: 4.155 ± 0.07	9.948AlaArg: 9.948 ± 0.105	7.264AlaSer: 7.264 ± 0.102	6.644AlaThr: 6.644 ± 0.081	9.513AlaVal: 9.513 ± 0.103	1.591AlaTrp: 1.591 ± 0.035	2.667AlaTyr: 2.667 ± 0.043	0.0AlaXaa: 0.0 ± 0.0
Cys	0.976CysAla: 0.976 ± 0.033	0.113CysCys: 0.113 ± 0.01	0.495CysAsp: 0.495 ± 0.02	0.474CysGlu: 0.474 ± 0.017	0.289CysPhe: 0.289 ± 0.014	0.935CysGly: 0.935 ± 0.031	0.214CysHis: 0.214 ± 0.013	0.366CysIle: 0.366 ± 0.016	0.178CysLys: 0.178 ± 0.012	0.916CysLeu: 0.916 ± 0.03	0.148CysMet: 0.148 ± 0.009	0.202CysAsn: 0.202 ± 0.011	0.467CysPro: 0.467 ± 0.02	0.208CysGln: 0.208 ± 0.013	0.708CysArg: 0.708 ± 0.023	0.435CysSer: 0.435 ± 0.019	0.423CysThr: 0.423 ± 0.017	0.578CysVal: 0.578 ± 0.02	0.1CysTrp: 0.1 ± 0.008	0.157CysTyr: 0.157 ± 0.011	0.0CysXaa: 0.0 ± 0.0
Asp	7.501AspAla: 7.501 ± 0.079	0.429AspCys: 0.429 ± 0.02	3.095AspAsp: 3.095 ± 0.061	3.539AspGlu: 3.539 ± 0.054	1.842AspPhe: 1.842 ± 0.041	5.437AspGly: 5.437 ± 0.076	1.151AspHis: 1.151 ± 0.03	2.503AspIle: 2.503 ± 0.04	1.502AspLys: 1.502 ± 0.036	6.135AspLeu: 6.135 ± 0.076	1.019AspMet: 1.019 ± 0.033	1.053AspAsn: 1.053 ± 0.029	3.717AspPro: 3.717 ± 0.057	1.518AspGln: 1.518 ± 0.037	4.673AspArg: 4.673 ± 0.066	1.965AspSer: 1.965 ± 0.041	2.653AspThr: 2.653 ± 0.048	4.096AspVal: 4.096 ± 0.06	0.829AspTrp: 0.829 ± 0.026	1.233AspTyr: 1.233 ± 0.034	0.0AspXaa: 0.0 ± 0.0
Glu	8.87GluAla: 8.87 ± 0.106	0.369GluCys: 0.369 ± 0.014	2.681GluAsp: 2.681 ± 0.047	3.137GluGlu: 3.137 ± 0.054	1.524GluPhe: 1.524 ± 0.032	4.655GluGly: 4.655 ± 0.061	1.233GluHis: 1.233 ± 0.03	3.262GluIle: 3.262 ± 0.053	1.95GluLys: 1.95 ± 0.048	4.658GluLeu: 4.658 ± 0.056	1.284GluMet: 1.284 ± 0.037	1.333GluAsn: 1.333 ± 0.032	3.302GluPro: 3.302 ± 0.062	1.88GluGln: 1.88 ± 0.042	5.76GluArg: 5.76 ± 0.086	2.426GluSer: 2.426 ± 0.045	3.768GluThr: 3.768 ± 0.056	3.68GluVal: 3.68 ± 0.054	0.606GluTrp: 0.606 ± 0.02	0.839GluTyr: 0.839 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	4.552PheAla: 4.552 ± 0.072	0.363PheCys: 0.363 ± 0.017	2.391PheAsp: 2.391 ± 0.049	2.111PheGlu: 2.111 ± 0.039	1.234PhePhe: 1.234 ± 0.038	3.624PheGly: 3.624 ± 0.051	0.626PheHis: 0.626 ± 0.024	1.283PheIle: 1.283 ± 0.033	0.946PheLys: 0.946 ± 0.026	3.124PheLeu: 3.124 ± 0.053	0.648PheMet: 0.648 ± 0.024	0.848PheAsn: 0.848 ± 0.031	1.462PhePro: 1.462 ± 0.035	0.836PheGln: 0.836 ± 0.025	2.312PheArg: 2.312 ± 0.046	2.064PheSer: 2.064 ± 0.045	1.863PheThr: 1.863 ± 0.041	2.709PheVal: 2.709 ± 0.045	0.466PheTrp: 0.466 ± 0.019	0.824PheTyr: 0.824 ± 0.028	0.0PheXaa: 0.0 ± 0.0
Gly	10.01GlyAla: 10.01 ± 0.111	0.868GlyCys: 0.868 ± 0.026	4.457GlyAsp: 4.457 ± 0.074	4.879GlyGlu: 4.879 ± 0.079	3.674GlyPhe: 3.674 ± 0.052	7.7GlyGly: 7.7 ± 0.132	1.962GlyHis: 1.962 ± 0.042	4.554GlyIle: 4.554 ± 0.062	2.9GlyLys: 2.9 ± 0.054	9.882GlyLeu: 9.882 ± 0.094	2.051GlyMet: 2.051 ± 0.043	1.973GlyAsn: 1.973 ± 0.051	4.043GlyPro: 4.043 ± 0.061	2.918GlyGln: 2.918 ± 0.053	7.24GlyArg: 7.24 ± 0.078	5.297GlySer: 5.297 ± 0.07	4.913GlyThr: 4.913 ± 0.07	5.983GlyVal: 5.983 ± 0.063	1.34GlyTrp: 1.34 ± 0.032	2.303GlyTyr: 2.303 ± 0.046	0.0GlyXaa: 0.0 ± 0.0
His	2.427HisAla: 2.427 ± 0.044	0.2HisCys: 0.2 ± 0.013	1.217HisAsp: 1.217 ± 0.036	1.009HisGlu: 1.009 ± 0.03	0.716HisPhe: 0.716 ± 0.023	2.027HisGly: 2.027 ± 0.042	0.6HisHis: 0.6 ± 0.027	0.757HisIle: 0.757 ± 0.021	0.435HisLys: 0.435 ± 0.019	2.062HisLeu: 2.062 ± 0.04	0.404HisMet: 0.404 ± 0.015	0.417HisAsn: 0.417 ± 0.018	1.385HisPro: 1.385 ± 0.036	0.495HisGln: 0.495 ± 0.019	1.549HisArg: 1.549 ± 0.038	0.854HisSer: 0.854 ± 0.03	0.778HisThr: 0.778 ± 0.026	1.492HisVal: 1.492 ± 0.033	0.321HisTrp: 0.321 ± 0.016	0.469HisTyr: 0.469 ± 0.018	0.0HisXaa: 0.0 ± 0.0
Ile	7.126IleAla: 7.126 ± 0.074	0.456IleCys: 0.456 ± 0.021	3.332IleAsp: 3.332 ± 0.053	3.513IleGlu: 3.513 ± 0.052	1.299IlePhe: 1.299 ± 0.032	4.858IleGly: 4.858 ± 0.064	0.817IleHis: 0.817 ± 0.025	1.785IleIle: 1.785 ± 0.046	1.25IleLys: 1.25 ± 0.035	4.112IleLeu: 4.112 ± 0.057	0.767IleMet: 0.767 ± 0.026	1.115IleAsn: 1.115 ± 0.032	2.258IlePro: 2.258 ± 0.048	1.194IleGln: 1.194 ± 0.027	3.256IleArg: 3.256 ± 0.049	2.477IleSer: 2.477 ± 0.051	2.496IleThr: 2.496 ± 0.048	4.162IleVal: 4.162 ± 0.061	0.486IleTrp: 0.486 ± 0.021	0.958IleTyr: 0.958 ± 0.03	0.0IleXaa: 0.0 ± 0.0
Lys	4.33LysAla: 4.33 ± 0.088	0.135LysCys: 0.135 ± 0.01	1.612LysAsp: 1.612 ± 0.043	1.413LysGlu: 1.413 ± 0.04	0.708LysPhe: 0.708 ± 0.023	2.596LysGly: 2.596 ± 0.046	0.515LysHis: 0.515 ± 0.021	1.434LysIle: 1.434 ± 0.039	1.113LysLys: 1.113 ± 0.039	2.794LysLeu: 2.794 ± 0.053	0.568LysMet: 0.568 ± 0.023	0.773LysAsn: 0.773 ± 0.029	2.292LysPro: 2.292 ± 0.047	0.868LysGln: 0.868 ± 0.029	2.421LysArg: 2.421 ± 0.038	1.66LysSer: 1.66 ± 0.047	1.786LysThr: 1.786 ± 0.037	2.252LysVal: 2.252 ± 0.058	0.256LysTrp: 0.256 ± 0.017	0.523LysTyr: 0.523 ± 0.02	0.0LysXaa: 0.0 ± 0.0
Leu	14.759LeuAla: 14.759 ± 0.156	0.929LeuCys: 0.929 ± 0.03	6.355LeuAsp: 6.355 ± 0.076	4.583LeuGlu: 4.583 ± 0.068	3.323LeuPhe: 3.323 ± 0.061	8.632LeuGly: 8.632 ± 0.089	1.848LeuHis: 1.848 ± 0.037	4.78LeuIle: 4.78 ± 0.067	3.374LeuLys: 3.374 ± 0.056	8.875LeuLeu: 8.875 ± 0.115	2.075LeuMet: 2.075 ± 0.039	2.303LeuAsn: 2.303 ± 0.048	5.871LeuPro: 5.871 ± 0.068	2.581LeuGln: 2.581 ± 0.045	7.527LeuArg: 7.527 ± 0.083	6.31LeuSer: 6.31 ± 0.085	5.79LeuThr: 5.79 ± 0.068	7.829LeuVal: 7.829 ± 0.082	1.06LeuTrp: 1.06 ± 0.027	1.927LeuTyr: 1.927 ± 0.041	0.0LeuXaa: 0.0 ± 0.0
Met	2.754MetAla: 2.754 ± 0.042	0.122MetCys: 0.122 ± 0.009	0.85MetAsp: 0.85 ± 0.025	0.945MetGlu: 0.945 ± 0.027	0.606MetPhe: 0.606 ± 0.023	1.496MetGly: 1.496 ± 0.039	0.405MetHis: 0.405 ± 0.019	1.131MetIle: 1.131 ± 0.029	0.795MetLys: 0.795 ± 0.027	2.225MetLeu: 2.225 ± 0.042	0.56MetMet: 0.56 ± 0.026	0.634MetAsn: 0.634 ± 0.022	1.624MetPro: 1.624 ± 0.036	0.741MetGln: 0.741 ± 0.02	1.957MetArg: 1.957 ± 0.034	1.515MetSer: 1.515 ± 0.033	1.559MetThr: 1.559 ± 0.038	1.426MetVal: 1.426 ± 0.032	0.137MetTrp: 0.137 ± 0.009	0.23MetTyr: 0.23 ± 0.011	0.0MetXaa: 0.0 ± 0.0
Asn	3.024AsnAla: 3.024 ± 0.046	0.203AsnCys: 0.203 ± 0.012	1.245AsnAsp: 1.245 ± 0.04	1.118AsnGlu: 1.118 ± 0.031	0.776AsnPhe: 0.776 ± 0.027	2.231AsnGly: 2.231 ± 0.056	0.441AsnHis: 0.441 ± 0.022	1.022AsnIle: 1.022 ± 0.032	0.593AsnLys: 0.593 ± 0.026	2.381AsnLeu: 2.381 ± 0.048	0.458AsnMet: 0.458 ± 0.019	0.588AsnAsn: 0.588 ± 0.027	1.694AsnPro: 1.694 ± 0.04	0.654AsnGln: 0.654 ± 0.024	1.738AsnArg: 1.738 ± 0.04	1.052AsnSer: 1.052 ± 0.03	1.12AsnThr: 1.12 ± 0.032	1.716AsnVal: 1.716 ± 0.036	0.333AsnTrp: 0.333 ± 0.016	0.539AsnTyr: 0.539 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.321ProAla: 7.321 ± 0.097	0.362ProCys: 0.362 ± 0.015	3.782ProAsp: 3.782 ± 0.061	3.89ProGlu: 3.89 ± 0.063	1.985ProPhe: 1.985 ± 0.038	5.187ProGly: 5.187 ± 0.072	1.063ProHis: 1.063 ± 0.027	2.438ProIle: 2.438 ± 0.046	1.717ProLys: 1.717 ± 0.037	4.898ProLeu: 4.898 ± 0.07	1.165ProMet: 1.165 ± 0.029	1.379ProAsn: 1.379 ± 0.038	3.116ProPro: 3.116 ± 0.066	1.593ProGln: 1.593 ± 0.038	3.543ProArg: 3.543 ± 0.059	3.274ProSer: 3.274 ± 0.054	2.721ProThr: 2.721 ± 0.05	4.472ProVal: 4.472 ± 0.06	0.701ProTrp: 0.701 ± 0.022	1.14ProTyr: 1.14 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.463GlnAla: 4.463 ± 0.068	0.172GlnCys: 0.172 ± 0.012	1.414GlnAsp: 1.414 ± 0.034	1.345GlnGlu: 1.345 ± 0.033	0.892GlnPhe: 0.892 ± 0.026	2.503GlnGly: 2.503 ± 0.044	0.574GlnHis: 0.574 ± 0.021	1.607GlnIle: 1.607 ± 0.036	0.886GlnLys: 0.886 ± 0.029	2.43GlnLeu: 2.43 ± 0.041	0.651GlnMet: 0.651 ± 0.023	0.734GlnAsn: 0.734 ± 0.026	1.838GlnPro: 1.838 ± 0.043	1.062GlnGln: 1.062 ± 0.048	2.379GlnArg: 2.379 ± 0.048	1.696GlnSer: 1.696 ± 0.038	1.729GlnThr: 1.729 ± 0.04	2.178GlnVal: 2.178 ± 0.049	0.304GlnTrp: 0.304 ± 0.017	0.518GlnTyr: 0.518 ± 0.018	0.0GlnXaa: 0.0 ± 0.0
Arg	9.144ArgAla: 9.144 ± 0.104	0.549ArgCys: 0.549 ± 0.021	4.347ArgAsp: 4.347 ± 0.057	4.7ArgGlu: 4.7 ± 0.066	3.01ArgPhe: 3.01 ± 0.046	5.616ArgGly: 5.616 ± 0.067	1.814ArgHis: 1.814 ± 0.038	4.206ArgIle: 4.206 ± 0.057	2.17ArgLys: 2.17 ± 0.047	8.836ArgLeu: 8.836 ± 0.099	1.821ArgMet: 1.821 ± 0.036	1.718ArgAsn: 1.718 ± 0.037	4.255ArgPro: 4.255 ± 0.063	2.52ArgGln: 2.52 ± 0.048	6.738ArgArg: 6.738 ± 0.096	4.398ArgSer: 4.398 ± 0.061	3.779ArgThr: 3.779 ± 0.063	5.368ArgVal: 5.368 ± 0.061	1.019ArgTrp: 1.019 ± 0.027	1.722ArgTyr: 1.722 ± 0.038	0.0ArgXaa: 0.0 ± 0.0
Ser	6.743SerAla: 6.743 ± 0.083	0.46SerCys: 0.46 ± 0.02	2.995SerAsp: 2.995 ± 0.054	3.062SerGlu: 3.062 ± 0.057	2.094SerPhe: 2.094 ± 0.04	5.752SerGly: 5.752 ± 0.076	1.011SerHis: 1.011 ± 0.028	2.491SerIle: 2.491 ± 0.052	1.502SerLys: 1.502 ± 0.036	5.781SerLeu: 5.781 ± 0.067	1.124SerMet: 1.124 ± 0.028	1.304SerAsn: 1.304 ± 0.035	2.905SerPro: 2.905 ± 0.05	1.587SerGln: 1.587 ± 0.038	4.079SerArg: 4.079 ± 0.059	3.042SerSer: 3.042 ± 0.062	2.688SerThr: 2.688 ± 0.048	4.064SerVal: 4.064 ± 0.053	0.705SerTrp: 0.705 ± 0.023	1.247SerTyr: 1.247 ± 0.029	0.0SerXaa: 0.0 ± 0.0
Thr	6.721ThrAla: 6.721 ± 0.089	0.46ThrCys: 0.46 ± 0.019	2.722ThrAsp: 2.722 ± 0.047	2.69ThrGlu: 2.69 ± 0.044	1.869ThrPhe: 1.869 ± 0.043	5.293ThrGly: 5.293 ± 0.067	0.991ThrHis: 0.991 ± 0.034	2.834ThrIle: 2.834 ± 0.05	1.49ThrLys: 1.49 ± 0.035	6.021ThrLeu: 6.021 ± 0.074	1.086ThrMet: 1.086 ± 0.031	1.259ThrAsn: 1.259 ± 0.034	3.367ThrPro: 3.367 ± 0.054	1.436ThrGln: 1.436 ± 0.034	3.612ThrArg: 3.612 ± 0.053	2.79ThrSer: 2.79 ± 0.048	2.846ThrThr: 2.846 ± 0.052	4.568ThrVal: 4.568 ± 0.063	0.678ThrTrp: 0.678 ± 0.021	1.164ThrTyr: 1.164 ± 0.033	0.0ThrXaa: 0.0 ± 0.0
Val	9.78ValAla: 9.78 ± 0.093	0.644ValCys: 0.644 ± 0.022	4.013ValAsp: 4.013 ± 0.064	4.754ValGlu: 4.754 ± 0.064	2.585ValPhe: 2.585 ± 0.045	5.865ValGly: 5.865 ± 0.075	1.329ValHis: 1.329 ± 0.033	3.649ValIle: 3.649 ± 0.056	2.192ValLys: 2.192 ± 0.046	7.509ValLeu: 7.509 ± 0.089	1.712ValMet: 1.712 ± 0.039	1.69ValAsn: 1.69 ± 0.036	4.181ValPro: 4.181 ± 0.053	2.066ValGln: 2.066 ± 0.046	5.227ValArg: 5.227 ± 0.075	4.365ValSer: 4.365 ± 0.06	4.581ValThr: 4.581 ± 0.067	6.1ValVal: 6.1 ± 0.078	0.848ValTrp: 0.848 ± 0.031	1.392ValTyr: 1.392 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.179TrpAla: 1.179 ± 0.03	0.139TrpCys: 0.139 ± 0.011	0.572TrpAsp: 0.572 ± 0.019	0.462TrpGlu: 0.462 ± 0.019	0.501TrpPhe: 0.501 ± 0.021	0.785TrpGly: 0.785 ± 0.027	0.31TrpHis: 0.31 ± 0.014	0.626TrpIle: 0.626 ± 0.024	0.363TrpLys: 0.363 ± 0.019	1.622TrpLeu: 1.622 ± 0.039	0.303TrpMet: 0.303 ± 0.015	0.369TrpAsn: 0.369 ± 0.017	0.634TrpPro: 0.634 ± 0.023	0.498TrpGln: 0.498 ± 0.019	1.189TrpArg: 1.189 ± 0.026	0.766TrpSer: 0.766 ± 0.023	0.753TrpThr: 0.753 ± 0.029	0.75TrpVal: 0.75 ± 0.024	0.209TrpTrp: 0.209 ± 0.013	0.259TrpTyr: 0.259 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.547TyrAla: 2.547 ± 0.047	0.236TyrCys: 0.236 ± 0.012	1.373TyrAsp: 1.373 ± 0.033	1.209TyrGlu: 1.209 ± 0.028	0.782TyrPhe: 0.782 ± 0.024	2.249TyrGly: 2.249 ± 0.045	0.396TyrHis: 0.396 ± 0.016	0.704TyrIle: 0.704 ± 0.022	0.51TyrLys: 0.51 ± 0.022	2.01TyrLeu: 2.01 ± 0.039	0.34TyrMet: 0.34 ± 0.017	0.516TyrAsn: 0.516 ± 0.022	0.964TyrPro: 0.964 ± 0.026	0.565TyrGln: 0.565 ± 0.02	1.872TyrArg: 1.872 ± 0.036	0.986TyrSer: 0.986 ± 0.03	1.006TyrThr: 1.006 ± 0.03	1.546TyrVal: 1.546 ± 0.039	0.3TyrTrp: 0.3 ± 0.016	0.5TyrTyr: 0.5 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 4699 proteins (1334609 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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