Escherichia phage phiv205-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 70 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2F1E2|A0A5Q2F1E2_9CAUD Prophage antirepressor OS=Escherichia phage phiv205-1 OX=2663260 PE=4 SV=1
MM1 pKa = 8.29DD2 pKa = 4.56YY3 pKa = 11.03SQLSDD8 pKa = 3.47FEE10 pKa = 4.94INRR13 pKa = 11.84RR14 pKa = 11.84VCEE17 pKa = 3.94ALDD20 pKa = 3.63MEE22 pKa = 4.65EE23 pKa = 5.25HH24 pKa = 6.6FFIPDD29 pKa = 3.35DD30 pKa = 3.78EE31 pKa = 6.78ADD33 pKa = 3.93FDD35 pKa = 4.96SEE37 pKa = 4.28IPTDD41 pKa = 3.44EE42 pKa = 5.45RR43 pKa = 11.84GPIWQTQKK51 pKa = 10.79RR52 pKa = 11.84DD53 pKa = 3.4INGFRR58 pKa = 11.84SSNGNCFNPCNNPEE72 pKa = 4.17YY73 pKa = 9.77AWPIITEE80 pKa = 4.36SKK82 pKa = 10.3ISTMWMTAEE91 pKa = 4.77KK92 pKa = 9.78EE93 pKa = 3.78WCAWSGGDD101 pKa = 4.92LEE103 pKa = 4.56EE104 pKa = 5.89GCWEE108 pKa = 3.95WEE110 pKa = 4.32NIPGYY115 pKa = 7.82YY116 pKa = 9.84FCGEE120 pKa = 4.19SPLRR124 pKa = 11.84AAMIVFLMMQDD135 pKa = 3.31ANNAA139 pKa = 3.59

Molecular weight: 16.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2F5W1|A0A5Q2F5W1_9CAUD Uncharacterized protein OS=Escherichia phage phiv205-1 OX=2663260 PE=4 SV=1
MM1 pKa = 7.47NATIQTIPEE10 pKa = 4.26LLIQTRR16 pKa = 11.84GNQTEE21 pKa = 4.29VARR24 pKa = 11.84MLSCARR30 pKa = 11.84GTVLKK35 pKa = 10.59YY36 pKa = 10.74NRR38 pKa = 11.84DD39 pKa = 3.73SKK41 pKa = 11.2GEE43 pKa = 3.67RR44 pKa = 11.84HH45 pKa = 6.06VIVNGVLMVKK55 pKa = 9.6QGKK58 pKa = 9.51RR59 pKa = 11.84GRR61 pKa = 11.84PP62 pKa = 3.31

Molecular weight: 6.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

70

0

70

13150

39

917

187.9

21.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.529 ± 0.507

1.194 ± 0.144

6.16 ± 0.249

6.768 ± 0.431

3.141 ± 0.197

7.042 ± 0.363

1.719 ± 0.159

5.992 ± 0.245

6.395 ± 0.356

7.954 ± 0.326

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.928 ± 0.195

4.38 ± 0.227

3.817 ± 0.194

4.722 ± 0.328

6.015 ± 0.279

6.221 ± 0.218

5.217 ± 0.196

5.878 ± 0.295

1.589 ± 0.146

3.338 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski