Pasteurella multocida subsp. multocida str. Anand1_buffalo

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Pasteurella; Pasteurella multocida; Pasteurella multocida subsp. multocida

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3217 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J6CU62|J6CU62_PASMD Uncharacterized protein (Fragment) OS=Pasteurella multocida subsp. multocida str. Anand1_buffalo OX=1032868 GN=AAUPMB_03238 PE=4 SV=1
MM1 pKa = 7.5PKK3 pKa = 10.12VVFLPHH9 pKa = 7.15EE10 pKa = 4.3EE11 pKa = 4.16LCPEE15 pKa = 4.34GLALEE20 pKa = 4.38VAEE23 pKa = 5.51GEE25 pKa = 4.33NLLDD29 pKa = 4.0AALDD33 pKa = 3.65AGIDD37 pKa = 3.62IEE39 pKa = 4.4HH40 pKa = 7.02ACDD43 pKa = 3.74KK44 pKa = 11.19SCACTTCHH52 pKa = 6.2VIIRR56 pKa = 11.84EE57 pKa = 4.31GFDD60 pKa = 3.4SLNEE64 pKa = 4.18STDD67 pKa = 3.43MEE69 pKa = 7.16DD70 pKa = 5.84DD71 pKa = 3.93MLDD74 pKa = 3.68KK75 pKa = 11.06AWGLEE80 pKa = 3.77VDD82 pKa = 4.37SRR84 pKa = 11.84LSCQCLL90 pKa = 3.13

Molecular weight: 9.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J5KPD3|J5KPD3_PASMD Uncharacterized protein OS=Pasteurella multocida subsp. multocida str. Anand1_buffalo OX=1032868 GN=AAUPMB_10731 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.6RR12 pKa = 11.84SRR14 pKa = 11.84THH16 pKa = 6.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.1 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3217

0

3217

425246

13

768

132.2

14.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.567 ± 0.06

1.181 ± 0.025

4.884 ± 0.043

5.983 ± 0.057

4.341 ± 0.042

6.585 ± 0.051

2.373 ± 0.032

6.698 ± 0.049

5.832 ± 0.043

10.771 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.618 ± 0.026

4.231 ± 0.04

3.92 ± 0.037

5.052 ± 0.054

4.632 ± 0.044

5.755 ± 0.046

5.351 ± 0.039

6.849 ± 0.045

1.217 ± 0.022

3.149 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski