Malassezia pachydermatis
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4201 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M9VQT9|A0A0M9VQT9_9BASI DNA-directed RNA polymerase OS=Malassezia pachydermatis OX=77020 GN=Malapachy_3329 PE=3 SV=1
MM1 pKa = 7.3 AVSYY5 pKa = 10.71 YY6 pKa = 10.93 DD7 pKa = 4.0 EE8 pKa = 6.04 IEE10 pKa = 4.46 LEE12 pKa = 4.19 DD13 pKa = 3.78 MEE15 pKa = 5.34 FDD17 pKa = 3.6 EE18 pKa = 5.62 ARR20 pKa = 11.84 QAFTFPCPCGDD31 pKa = 3.24 KK32 pKa = 11.23 FEE34 pKa = 4.34 ITLAQLKK41 pKa = 10.46 DD42 pKa = 3.46 EE43 pKa = 4.6 EE44 pKa = 6.24 DD45 pKa = 3.22 IARR48 pKa = 11.84 CPSCTLIIRR57 pKa = 11.84 VIYY60 pKa = 10.39 DD61 pKa = 3.84 PLDD64 pKa = 4.18 LDD66 pKa = 4.12 LL67 pKa = 6.58
Molecular weight: 7.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.739
IPC2_protein 3.795
IPC_protein 3.732
Toseland 3.528
ProMoST 3.897
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.656
Rodwell 3.567
Grimsley 3.439
Solomon 3.694
Lehninger 3.656
Nozaki 3.846
DTASelect 4.037
Thurlkill 3.592
EMBOSS 3.668
Sillero 3.846
Patrickios 0.769
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.766
Protein with the highest isoelectric point:
>tr|A0A0M8MS53|A0A0M8MS53_9BASI Uncharacterized protein OS=Malassezia pachydermatis OX=77020 GN=Malapachy_1402 PE=4 SV=1
MM1 pKa = 7.6 LLLTSLVRR9 pKa = 11.84 GPRR12 pKa = 11.84 AAMMRR17 pKa = 11.84 AQPVSSLHH25 pKa = 5.46 TSAIVAAAPRR35 pKa = 11.84 PSAPRR40 pKa = 11.84 TKK42 pKa = 10.43 LKK44 pKa = 10.49 SHH46 pKa = 6.43 SAAKK50 pKa = 10.17 KK51 pKa = 9.74 RR52 pKa = 11.84 FFPVMGSGRR61 pKa = 11.84 GSPLSIKK68 pKa = 10.52 FKK70 pKa = 9.58 RR71 pKa = 11.84 TSSNKK76 pKa = 7.1 QHH78 pKa = 6.68 LNSGMSRR85 pKa = 11.84 VRR87 pKa = 11.84 LNRR90 pKa = 11.84 LSGTQVVSSGPVSRR104 pKa = 11.84 MLRR107 pKa = 11.84 RR108 pKa = 11.84 LLGPRR113 pKa = 11.84 LL114 pKa = 3.5
Molecular weight: 12.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.52
IPC2_protein 11.389
IPC_protein 12.983
Toseland 13.159
ProMoST 13.642
Dawson 13.159
Bjellqvist 13.144
Wikipedia 13.627
Rodwell 12.822
Grimsley 13.188
Solomon 13.642
Lehninger 13.554
Nozaki 13.159
DTASelect 13.144
Thurlkill 13.159
EMBOSS 13.656
Sillero 13.159
Patrickios 12.544
IPC_peptide 13.656
IPC2_peptide 12.632
IPC2.peptide.svr19 9.271
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4201
0
4201
2130625
51
4873
507.2
56.07
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.31 ± 0.049
1.125 ± 0.012
5.741 ± 0.026
6.074 ± 0.037
3.115 ± 0.025
5.828 ± 0.034
2.918 ± 0.017
3.866 ± 0.027
3.916 ± 0.038
9.539 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.802 ± 0.013
2.627 ± 0.025
6.368 ± 0.045
4.287 ± 0.021
6.607 ± 0.031
7.823 ± 0.041
6.33 ± 0.027
6.6 ± 0.026
1.48 ± 0.014
2.637 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here