Fragilariopsis cylindrus CCMP1102

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Bacillariophyceae; Bacillariophycidae; Bacillariales; Bacillariaceae; Fragilariopsis; Fragilariopsis cylindrus

Average proteome isoelectric point is 6.13

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 18075 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E7FDH6|A0A1E7FDH6_9STRA Uncharacterized protein OS=Fragilariopsis cylindrus CCMP1102 OX=635003 GN=FRACYDRAFT_225570 PE=4 SV=1
TT1 pKa = 7.48CSTIFDD7 pKa = 5.02LACSTDD13 pKa = 4.43GFDD16 pKa = 4.07TLCGLLTSFDD26 pKa = 4.15LKK28 pKa = 11.08DD29 pKa = 4.4AISGGSWTVFAPTNEE44 pKa = 4.21AFTKK48 pKa = 10.68LDD50 pKa = 3.69DD51 pKa = 4.72ALDD54 pKa = 3.93GGIASVSDD62 pKa = 3.82DD63 pKa = 4.43LLLKK67 pKa = 10.17ILQFHH72 pKa = 5.97VVDD75 pKa = 4.29DD76 pKa = 3.9QVLYY80 pKa = 11.1SDD82 pKa = 5.88DD83 pKa = 4.36LPCEE87 pKa = 4.06LGNNLVTTLSGKK99 pKa = 9.71DD100 pKa = 3.13SRR102 pKa = 11.84TFCEE106 pKa = 4.33DD107 pKa = 3.33DD108 pKa = 3.41VPYY111 pKa = 10.25GQKK114 pKa = 10.85GSGNDD119 pKa = 3.31EE120 pKa = 3.87VVKK123 pKa = 10.12FVGVDD128 pKa = 2.82IDD130 pKa = 3.53ACNGVVHH137 pKa = 6.68AVEE140 pKa = 5.27DD141 pKa = 3.71VLLFNLAPYY150 pKa = 8.53MM151 pKa = 4.28

Molecular weight: 16.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E7FTQ9|A0A1E7FTQ9_9STRA RING-type domain-containing protein OS=Fragilariopsis cylindrus CCMP1102 OX=635003 GN=FRACYDRAFT_235176 PE=4 SV=1
MM1 pKa = 7.93PSQKK5 pKa = 8.75TFRR8 pKa = 11.84TKK10 pKa = 9.67RR11 pKa = 11.84TLAKK15 pKa = 9.94KK16 pKa = 9.76QRR18 pKa = 11.84QNRR21 pKa = 11.84PLPHH25 pKa = 6.43WVRR28 pKa = 11.84LRR30 pKa = 11.84TDD32 pKa = 3.32NTIKK36 pKa = 9.96WNAKK40 pKa = 7.31RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.13WRR45 pKa = 11.84RR46 pKa = 11.84TKK48 pKa = 10.83LGLL51 pKa = 3.67

Molecular weight: 6.39 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

18047

28

18075

7486704

49

5870

414.2

46.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.382 ± 0.021

1.62 ± 0.008

6.663 ± 0.016

6.227 ± 0.016

3.519 ± 0.013

6.14 ± 0.019

2.146 ± 0.01

5.958 ± 0.013

6.085 ± 0.02

8.118 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.007

6.06 ± 0.022

4.31 ± 0.016

4.066 ± 0.016

4.973 ± 0.015

8.917 ± 0.026

6.659 ± 0.019

5.764 ± 0.015

1.11 ± 0.006

2.921 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski