Staphylococcus phage SPbeta-like

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 154 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0N9SK95|A0A0N9SK95_9CAUD DNA ligase OS=Staphylococcus phage SPbeta-like OX=1732063 GN=SPbetalike_00100 PE=4 SV=1
MM1 pKa = 7.26NALEE5 pKa = 4.12AAYY8 pKa = 9.99KK9 pKa = 10.61NVTFDD14 pKa = 3.69YY15 pKa = 11.46LEE17 pKa = 5.7DD18 pKa = 3.83NDD20 pKa = 5.18TYY22 pKa = 12.0DD23 pKa = 3.16NTQDD27 pKa = 3.25FNEE30 pKa = 5.24AIHH33 pKa = 5.24TTEE36 pKa = 5.58DD37 pKa = 3.26FLEE40 pKa = 4.22NLDD43 pKa = 3.53YY44 pKa = 11.6NFYY47 pKa = 10.94DD48 pKa = 3.89KK49 pKa = 11.06KK50 pKa = 11.13

Molecular weight: 6.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0N9RZK9|A0A0N9RZK9_9CAUD Ribonuclease Z OS=Staphylococcus phage SPbeta-like OX=1732063 GN=SPbetalike_00001 PE=4 SV=1
MM1 pKa = 7.57NYY3 pKa = 9.41FRR5 pKa = 11.84YY6 pKa = 9.8KK7 pKa = 10.41QFNKK11 pKa = 10.52DD12 pKa = 3.57VITVAVGYY20 pKa = 7.26YY21 pKa = 10.19LRR23 pKa = 11.84YY24 pKa = 9.13ALSYY28 pKa = 10.81RR29 pKa = 11.84DD30 pKa = 2.93ISEE33 pKa = 3.94ILRR36 pKa = 11.84GRR38 pKa = 11.84GVNVHH43 pKa = 6.39HH44 pKa = 6.08STVYY48 pKa = 10.25RR49 pKa = 11.84WVQEE53 pKa = 4.07YY54 pKa = 10.99APILYY59 pKa = 9.66QIWKK63 pKa = 8.98KK64 pKa = 9.18KK65 pKa = 9.22HH66 pKa = 5.01KK67 pKa = 9.46KK68 pKa = 10.03AYY70 pKa = 7.86YY71 pKa = 8.98KK72 pKa = 10.07WRR74 pKa = 11.84IDD76 pKa = 3.2EE77 pKa = 4.43TYY79 pKa = 10.77IKK81 pKa = 10.4IKK83 pKa = 10.66GKK85 pKa = 9.36WSYY88 pKa = 11.19LYY90 pKa = 10.34RR91 pKa = 11.84AIDD94 pKa = 3.87AEE96 pKa = 4.29GHH98 pKa = 5.95TLDD101 pKa = 3.11IWLRR105 pKa = 11.84KK106 pKa = 9.22QRR108 pKa = 11.84DD109 pKa = 3.34NHH111 pKa = 5.75SAYY114 pKa = 10.67AFIKK118 pKa = 10.5RR119 pKa = 11.84LIKK122 pKa = 10.62QFGKK126 pKa = 7.59PQKK129 pKa = 10.57VITDD133 pKa = 3.55QAPSTKK139 pKa = 10.21VAMAKK144 pKa = 9.93VIKK147 pKa = 10.25AFKK150 pKa = 10.28LKK152 pKa = 9.99PDD154 pKa = 3.64CHH156 pKa = 6.11CTSKK160 pKa = 11.14YY161 pKa = 10.57LNNLIEE167 pKa = 4.06QDD169 pKa = 3.16HH170 pKa = 5.7RR171 pKa = 11.84HH172 pKa = 4.88IKK174 pKa = 8.94VRR176 pKa = 11.84KK177 pKa = 7.48TRR179 pKa = 11.84YY180 pKa = 9.36QSINTAKK187 pKa = 9.07NTLKK191 pKa = 10.91GIEE194 pKa = 4.81CIYY197 pKa = 11.32ALYY200 pKa = 10.34KK201 pKa = 10.15KK202 pKa = 10.07NRR204 pKa = 11.84RR205 pKa = 11.84SLQIYY210 pKa = 8.68GFSPCHH216 pKa = 6.49EE217 pKa = 4.25ISIMLASS224 pKa = 3.71

Molecular weight: 26.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

154

0

154

37078

31

2757

240.8

27.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.17 ± 0.377

0.672 ± 0.074

6.503 ± 0.188

8.461 ± 0.257

4.153 ± 0.166

4.984 ± 0.234

1.785 ± 0.128

7.7 ± 0.231

9.494 ± 0.211

8.393 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.098

6.95 ± 0.165

2.147 ± 0.13

3.598 ± 0.245

3.824 ± 0.118

6.335 ± 0.251

6.098 ± 0.228

5.618 ± 0.158

0.771 ± 0.055

4.987 ± 0.187
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski