Amino acid dipepetide frequency for Gordonia phage Kurt

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.034AlaAla: 12.034 ± 1.147
0.732AlaCys: 0.732 ± 0.189
7.275AlaAsp: 7.275 ± 0.601
6.863AlaGlu: 6.863 ± 0.59
3.066AlaPhe: 3.066 ± 0.323
8.007AlaGly: 8.007 ± 0.713
1.922AlaHis: 1.922 ± 0.433
5.536AlaIle: 5.536 ± 0.641
5.125AlaLys: 5.125 ± 0.548
8.145AlaLeu: 8.145 ± 0.86
2.151AlaMet: 2.151 ± 0.308
2.974AlaAsn: 2.974 ± 0.311
4.484AlaPro: 4.484 ± 0.675
3.706AlaGln: 3.706 ± 0.563
6.177AlaArg: 6.177 ± 0.541
4.759AlaSer: 4.759 ± 0.566
6.314AlaThr: 6.314 ± 0.53
7.367AlaVal: 7.367 ± 0.459
1.601AlaTrp: 1.601 ± 0.278
2.745AlaTyr: 2.745 ± 0.291
0.0AlaXaa: 0.0 ± 0.0
Cys
0.732CysAla: 0.732 ± 0.197
0.046CysCys: 0.046 ± 0.049
0.824CysAsp: 0.824 ± 0.218
0.229CysGlu: 0.229 ± 0.09
0.137CysPhe: 0.137 ± 0.087
0.961CysGly: 0.961 ± 0.263
0.32CysHis: 0.32 ± 0.119
0.32CysIle: 0.32 ± 0.117
0.183CysLys: 0.183 ± 0.094
0.732CysLeu: 0.732 ± 0.22
0.0CysMet: 0.0 ± 0.0
0.229CysAsn: 0.229 ± 0.092
0.275CysPro: 0.275 ± 0.125
0.412CysGln: 0.412 ± 0.159
0.503CysArg: 0.503 ± 0.161
0.686CysSer: 0.686 ± 0.192
0.595CysThr: 0.595 ± 0.15
0.641CysVal: 0.641 ± 0.193
0.137CysTrp: 0.137 ± 0.083
0.412CysTyr: 0.412 ± 0.157
0.0CysXaa: 0.0 ± 0.0
Asp
6.818AspAla: 6.818 ± 0.401
0.412AspCys: 0.412 ± 0.151
6.772AspAsp: 6.772 ± 0.822
5.765AspGlu: 5.765 ± 0.749
2.379AspPhe: 2.379 ± 0.353
7.504AspGly: 7.504 ± 0.63
1.327AspHis: 1.327 ± 0.225
3.432AspIle: 3.432 ± 0.48
2.105AspLys: 2.105 ± 0.34
5.72AspLeu: 5.72 ± 0.421
1.601AspMet: 1.601 ± 0.229
2.288AspAsn: 2.288 ± 0.325
5.033AspPro: 5.033 ± 0.536
2.608AspGln: 2.608 ± 0.386
5.262AspArg: 5.262 ± 0.57
2.974AspSer: 2.974 ± 0.377
3.432AspThr: 3.432 ± 0.411
4.393AspVal: 4.393 ± 0.411
1.235AspTrp: 1.235 ± 0.199
1.922AspTyr: 1.922 ± 0.286
0.0AspXaa: 0.0 ± 0.0
Glu
5.445GluAla: 5.445 ± 0.496
0.503GluCys: 0.503 ± 0.172
4.255GluAsp: 4.255 ± 0.561
4.164GluGlu: 4.164 ± 0.591
2.517GluPhe: 2.517 ± 0.385
4.667GluGly: 4.667 ± 0.408
1.327GluHis: 1.327 ± 0.205
4.164GluIle: 4.164 ± 0.368
2.883GluLys: 2.883 ± 0.444
5.216GluLeu: 5.216 ± 0.548
1.556GluMet: 1.556 ± 0.254
2.059GluAsn: 2.059 ± 0.371
3.02GluPro: 3.02 ± 0.523
1.922GluGln: 1.922 ± 0.255
5.811GluArg: 5.811 ± 0.635
3.66GluSer: 3.66 ± 0.458
3.706GluThr: 3.706 ± 0.425
4.667GluVal: 4.667 ± 0.427
1.464GluTrp: 1.464 ± 0.285
1.83GluTyr: 1.83 ± 0.352
0.0GluXaa: 0.0 ± 0.0
Phe
2.837PheAla: 2.837 ± 0.37
0.366PheCys: 0.366 ± 0.123
3.066PheAsp: 3.066 ± 0.425
2.608PheGlu: 2.608 ± 0.328
0.869PhePhe: 0.869 ± 0.203
2.928PheGly: 2.928 ± 0.334
0.732PheHis: 0.732 ± 0.162
1.281PheIle: 1.281 ± 0.206
0.915PheLys: 0.915 ± 0.187
2.288PheLeu: 2.288 ± 0.403
0.549PheMet: 0.549 ± 0.127
0.686PheAsn: 0.686 ± 0.132
1.647PhePro: 1.647 ± 0.31
0.824PheGln: 0.824 ± 0.153
2.562PheArg: 2.562 ± 0.344
1.556PheSer: 1.556 ± 0.249
1.968PheThr: 1.968 ± 0.272
1.418PheVal: 1.418 ± 0.273
0.778PheTrp: 0.778 ± 0.195
0.732PheTyr: 0.732 ± 0.171
0.0PheXaa: 0.0 ± 0.0
Gly
7.55GlyAla: 7.55 ± 0.831
0.778GlyCys: 0.778 ± 0.236
5.994GlyAsp: 5.994 ± 0.54
5.491GlyGlu: 5.491 ± 0.493
2.425GlyPhe: 2.425 ± 0.295
7.87GlyGly: 7.87 ± 1.329
1.876GlyHis: 1.876 ± 0.261
3.752GlyIle: 3.752 ± 0.659
3.844GlyLys: 3.844 ± 0.401
6.589GlyLeu: 6.589 ± 0.645
2.242GlyMet: 2.242 ± 0.312
2.562GlyAsn: 2.562 ± 0.422
4.621GlyPro: 4.621 ± 0.55
2.883GlyGln: 2.883 ± 0.4
6.406GlyArg: 6.406 ± 0.478
5.079GlySer: 5.079 ± 0.611
5.399GlyThr: 5.399 ± 0.603
6.131GlyVal: 6.131 ± 0.645
1.693GlyTrp: 1.693 ± 0.268
3.02GlyTyr: 3.02 ± 0.42
0.0GlyXaa: 0.0 ± 0.0
His
1.464HisAla: 1.464 ± 0.307
0.137HisCys: 0.137 ± 0.087
1.144HisAsp: 1.144 ± 0.321
0.778HisGlu: 0.778 ± 0.219
0.595HisPhe: 0.595 ± 0.15
1.647HisGly: 1.647 ± 0.283
0.366HisHis: 0.366 ± 0.127
0.732HisIle: 0.732 ± 0.148
0.458HisLys: 0.458 ± 0.177
1.327HisLeu: 1.327 ± 0.309
0.32HisMet: 0.32 ± 0.12
0.412HisAsn: 0.412 ± 0.128
1.83HisPro: 1.83 ± 0.269
0.412HisGln: 0.412 ± 0.127
1.968HisArg: 1.968 ± 0.31
1.052HisSer: 1.052 ± 0.233
0.686HisThr: 0.686 ± 0.156
1.922HisVal: 1.922 ± 0.377
0.503HisTrp: 0.503 ± 0.136
0.961HisTyr: 0.961 ± 0.229
0.0HisXaa: 0.0 ± 0.0
Ile
6.406IleAla: 6.406 ± 0.629
0.229IleCys: 0.229 ± 0.112
3.889IleAsp: 3.889 ± 0.405
4.301IleGlu: 4.301 ± 0.508
0.869IlePhe: 0.869 ± 0.229
4.484IleGly: 4.484 ± 0.6
0.915IleHis: 0.915 ± 0.215
1.876IleIle: 1.876 ± 0.248
2.151IleLys: 2.151 ± 0.407
3.706IleLeu: 3.706 ± 0.455
0.778IleMet: 0.778 ± 0.206
2.379IleAsn: 2.379 ± 0.339
2.608IlePro: 2.608 ± 0.304
1.51IleGln: 1.51 ± 0.261
2.7IleArg: 2.7 ± 0.319
2.928IleSer: 2.928 ± 0.357
2.928IleThr: 2.928 ± 0.354
2.837IleVal: 2.837 ± 0.405
0.732IleTrp: 0.732 ± 0.186
1.19IleTyr: 1.19 ± 0.253
0.0IleXaa: 0.0 ± 0.0
Lys
3.386LysAla: 3.386 ± 0.464
0.32LysCys: 0.32 ± 0.129
2.974LysAsp: 2.974 ± 0.435
1.739LysGlu: 1.739 ± 0.315
1.235LysPhe: 1.235 ± 0.234
2.928LysGly: 2.928 ± 0.437
0.595LysHis: 0.595 ± 0.14
1.876LysIle: 1.876 ± 0.288
2.196LysLys: 2.196 ± 0.464
3.294LysLeu: 3.294 ± 0.403
1.281LysMet: 1.281 ± 0.215
0.961LysAsn: 0.961 ± 0.221
2.608LysPro: 2.608 ± 0.422
1.373LysGln: 1.373 ± 0.262
3.34LysArg: 3.34 ± 0.454
2.105LysSer: 2.105 ± 0.337
3.02LysThr: 3.02 ± 0.395
3.477LysVal: 3.477 ± 0.308
0.641LysTrp: 0.641 ± 0.171
1.007LysTyr: 1.007 ± 0.232
0.0LysXaa: 0.0 ± 0.0
Leu
8.007LeuAla: 8.007 ± 0.675
0.915LeuCys: 0.915 ± 0.24
6.635LeuAsp: 6.635 ± 0.653
3.569LeuGlu: 3.569 ± 0.425
2.288LeuPhe: 2.288 ± 0.296
6.68LeuGly: 6.68 ± 0.753
0.961LeuHis: 0.961 ± 0.252
3.706LeuIle: 3.706 ± 0.407
2.7LeuLys: 2.7 ± 0.313
4.667LeuLeu: 4.667 ± 0.448
1.739LeuMet: 1.739 ± 0.243
2.196LeuAsn: 2.196 ± 0.33
4.759LeuPro: 4.759 ± 0.443
2.105LeuGln: 2.105 ± 0.295
6.086LeuArg: 6.086 ± 0.47
3.889LeuSer: 3.889 ± 0.436
5.445LeuThr: 5.445 ± 0.581
5.216LeuVal: 5.216 ± 0.383
1.281LeuTrp: 1.281 ± 0.258
1.418LeuTyr: 1.418 ± 0.226
0.0LeuXaa: 0.0 ± 0.0
Met
2.928MetAla: 2.928 ± 0.561
0.046MetCys: 0.046 ± 0.048
0.915MetAsp: 0.915 ± 0.164
1.556MetGlu: 1.556 ± 0.251
0.732MetPhe: 0.732 ± 0.176
0.961MetGly: 0.961 ± 0.256
0.503MetHis: 0.503 ± 0.144
1.052MetIle: 1.052 ± 0.212
0.869MetLys: 0.869 ± 0.202
1.647MetLeu: 1.647 ± 0.209
0.458MetMet: 0.458 ± 0.146
0.869MetAsn: 0.869 ± 0.204
1.739MetPro: 1.739 ± 0.312
0.641MetGln: 0.641 ± 0.168
1.647MetArg: 1.647 ± 0.234
1.51MetSer: 1.51 ± 0.29
2.013MetThr: 2.013 ± 0.285
1.098MetVal: 1.098 ± 0.187
0.503MetTrp: 0.503 ± 0.163
0.549MetTyr: 0.549 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
2.7AsnAla: 2.7 ± 0.268
0.366AsnCys: 0.366 ± 0.11
2.151AsnAsp: 2.151 ± 0.323
1.83AsnGlu: 1.83 ± 0.228
1.007AsnPhe: 1.007 ± 0.171
3.432AsnGly: 3.432 ± 0.372
0.458AsnHis: 0.458 ± 0.156
1.19AsnIle: 1.19 ± 0.23
0.961AsnLys: 0.961 ± 0.224
1.876AsnLeu: 1.876 ± 0.282
0.549AsnMet: 0.549 ± 0.173
1.144AsnAsn: 1.144 ± 0.184
2.242AsnPro: 2.242 ± 0.346
0.915AsnGln: 0.915 ± 0.175
2.288AsnArg: 2.288 ± 0.264
1.418AsnSer: 1.418 ± 0.201
2.334AsnThr: 2.334 ± 0.348
2.151AsnVal: 2.151 ± 0.303
0.824AsnTrp: 0.824 ± 0.184
1.052AsnTyr: 1.052 ± 0.229
0.0AsnXaa: 0.0 ± 0.0
Pro
5.765ProAla: 5.765 ± 0.572
0.183ProCys: 0.183 ± 0.084
4.804ProAsp: 4.804 ± 0.489
4.255ProGlu: 4.255 ± 0.62
1.83ProPhe: 1.83 ± 0.275
4.85ProGly: 4.85 ± 0.557
0.915ProHis: 0.915 ± 0.195
3.249ProIle: 3.249 ± 0.457
2.791ProLys: 2.791 ± 0.416
4.027ProLeu: 4.027 ± 0.439
1.556ProMet: 1.556 ± 0.302
1.876ProAsn: 1.876 ± 0.366
2.425ProPro: 2.425 ± 0.368
1.418ProGln: 1.418 ± 0.194
4.621ProArg: 4.621 ± 0.636
3.34ProSer: 3.34 ± 0.449
4.072ProThr: 4.072 ± 0.437
4.164ProVal: 4.164 ± 0.367
0.778ProTrp: 0.778 ± 0.157
1.007ProTyr: 1.007 ± 0.179
0.0ProXaa: 0.0 ± 0.0
Gln
3.935GlnAla: 3.935 ± 0.367
0.183GlnCys: 0.183 ± 0.09
1.327GlnAsp: 1.327 ± 0.319
1.464GlnGlu: 1.464 ± 0.272
1.19GlnPhe: 1.19 ± 0.254
1.876GlnGly: 1.876 ± 0.248
0.229GlnHis: 0.229 ± 0.095
1.876GlnIle: 1.876 ± 0.323
0.961GlnLys: 0.961 ± 0.237
2.745GlnLeu: 2.745 ± 0.405
1.052GlnMet: 1.052 ± 0.191
1.144GlnAsn: 1.144 ± 0.247
2.059GlnPro: 2.059 ± 0.314
1.418GlnGln: 1.418 ± 0.263
2.837GlnArg: 2.837 ± 0.374
1.693GlnSer: 1.693 ± 0.266
1.876GlnThr: 1.876 ± 0.351
2.471GlnVal: 2.471 ± 0.358
0.641GlnTrp: 0.641 ± 0.173
0.595GlnTyr: 0.595 ± 0.146
0.0GlnXaa: 0.0 ± 0.0
Arg
7.87ArgAla: 7.87 ± 0.683
1.281ArgCys: 1.281 ± 0.27
4.942ArgAsp: 4.942 ± 0.554
5.125ArgGlu: 5.125 ± 0.5
2.608ArgPhe: 2.608 ± 0.43
6.269ArgGly: 6.269 ± 0.585
1.556ArgHis: 1.556 ± 0.317
3.844ArgIle: 3.844 ± 0.4
4.21ArgLys: 4.21 ± 0.443
4.759ArgLeu: 4.759 ± 0.493
2.105ArgMet: 2.105 ± 0.31
2.288ArgAsn: 2.288 ± 0.328
3.752ArgPro: 3.752 ± 0.492
2.059ArgGln: 2.059 ± 0.38
6.497ArgArg: 6.497 ± 0.619
2.928ArgSer: 2.928 ± 0.39
4.53ArgThr: 4.53 ± 0.494
5.948ArgVal: 5.948 ± 0.632
1.876ArgTrp: 1.876 ± 0.277
1.968ArgTyr: 1.968 ± 0.231
0.0ArgXaa: 0.0 ± 0.0
Ser
6.314SerAla: 6.314 ± 0.583
0.458SerCys: 0.458 ± 0.135
3.889SerAsp: 3.889 ± 0.446
3.615SerGlu: 3.615 ± 0.43
1.098SerPhe: 1.098 ± 0.204
5.125SerGly: 5.125 ± 0.55
1.007SerHis: 1.007 ± 0.222
2.837SerIle: 2.837 ± 0.314
1.784SerLys: 1.784 ± 0.273
3.294SerLeu: 3.294 ± 0.36
1.418SerMet: 1.418 ± 0.243
1.464SerAsn: 1.464 ± 0.27
3.02SerPro: 3.02 ± 0.313
2.013SerGln: 2.013 ± 0.418
3.66SerArg: 3.66 ± 0.349
3.02SerSer: 3.02 ± 0.439
3.02SerThr: 3.02 ± 0.387
4.255SerVal: 4.255 ± 0.475
0.961SerTrp: 0.961 ± 0.2
0.869SerTyr: 0.869 ± 0.147
0.0SerXaa: 0.0 ± 0.0
Thr
5.582ThrAla: 5.582 ± 0.532
0.412ThrCys: 0.412 ± 0.145
3.615ThrAsp: 3.615 ± 0.368
3.523ThrGlu: 3.523 ± 0.401
2.105ThrPhe: 2.105 ± 0.314
7.001ThrGly: 7.001 ± 0.558
1.464ThrHis: 1.464 ± 0.259
3.706ThrIle: 3.706 ± 0.373
2.745ThrLys: 2.745 ± 0.344
5.353ThrLeu: 5.353 ± 0.611
1.052ThrMet: 1.052 ± 0.223
1.556ThrAsn: 1.556 ± 0.26
4.438ThrPro: 4.438 ± 0.445
1.83ThrGln: 1.83 ± 0.259
4.118ThrArg: 4.118 ± 0.459
3.386ThrSer: 3.386 ± 0.37
4.576ThrThr: 4.576 ± 0.517
4.85ThrVal: 4.85 ± 0.728
1.327ThrTrp: 1.327 ± 0.237
1.556ThrTyr: 1.556 ± 0.276
0.0ThrXaa: 0.0 ± 0.0
Val
7.275ValAla: 7.275 ± 0.749
0.686ValCys: 0.686 ± 0.219
5.353ValAsp: 5.353 ± 0.461
5.536ValGlu: 5.536 ± 0.543
2.151ValPhe: 2.151 ± 0.247
5.674ValGly: 5.674 ± 0.62
1.052ValHis: 1.052 ± 0.233
2.928ValIle: 2.928 ± 0.385
1.922ValLys: 1.922 ± 0.285
4.621ValLeu: 4.621 ± 0.368
1.144ValMet: 1.144 ± 0.231
2.334ValAsn: 2.334 ± 0.284
4.255ValPro: 4.255 ± 0.43
2.196ValGln: 2.196 ± 0.358
5.857ValArg: 5.857 ± 0.515
4.255ValSer: 4.255 ± 0.408
5.536ValThr: 5.536 ± 0.535
4.896ValVal: 4.896 ± 0.423
1.327ValTrp: 1.327 ± 0.301
2.196ValTyr: 2.196 ± 0.364
0.0ValXaa: 0.0 ± 0.0
Trp
1.784TrpAla: 1.784 ± 0.308
0.092TrpCys: 0.092 ± 0.075
1.373TrpAsp: 1.373 ± 0.254
1.098TrpGlu: 1.098 ± 0.243
0.686TrpPhe: 0.686 ± 0.17
1.144TrpGly: 1.144 ± 0.193
0.686TrpHis: 0.686 ± 0.169
1.235TrpIle: 1.235 ± 0.255
0.869TrpLys: 0.869 ± 0.176
1.876TrpLeu: 1.876 ± 0.371
0.183TrpMet: 0.183 ± 0.093
0.686TrpAsn: 0.686 ± 0.206
0.915TrpPro: 0.915 ± 0.227
0.824TrpGln: 0.824 ± 0.195
1.464TrpArg: 1.464 ± 0.281
1.281TrpSer: 1.281 ± 0.357
1.373TrpThr: 1.373 ± 0.255
1.19TrpVal: 1.19 ± 0.213
0.915TrpTrp: 0.915 ± 0.222
0.229TrpTyr: 0.229 ± 0.097
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.151TyrAla: 2.151 ± 0.293
0.229TyrCys: 0.229 ± 0.107
1.784TyrAsp: 1.784 ± 0.289
1.327TyrGlu: 1.327 ± 0.241
0.824TyrPhe: 0.824 ± 0.202
2.242TyrGly: 2.242 ± 0.366
0.503TyrHis: 0.503 ± 0.165
0.824TyrIle: 0.824 ± 0.206
0.686TyrLys: 0.686 ± 0.19
2.379TyrLeu: 2.379 ± 0.297
0.458TyrMet: 0.458 ± 0.133
0.778TyrAsn: 0.778 ± 0.203
2.196TyrPro: 2.196 ± 0.314
0.503TyrGln: 0.503 ± 0.14
2.608TyrArg: 2.608 ± 0.303
1.601TyrSer: 1.601 ± 0.272
1.373TyrThr: 1.373 ± 0.236
2.105TyrVal: 2.105 ± 0.331
0.686TyrTrp: 0.686 ± 0.231
0.549TyrTyr: 0.549 ± 0.181
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 95 proteins (21856 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski