Gammapapillomavirus 22
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2D2AM26|A0A2D2AM26_9PAPI E4 OS=Gammapapillomavirus 22 OX=1961679 GN=E4 PE=4 SV=1
MM1 pKa = 7.75 IGPDD5 pKa = 3.46 VKK7 pKa = 11.1 SGDD10 pKa = 3.4 IEE12 pKa = 5.61 LDD14 pKa = 3.55 LEE16 pKa = 4.64 SLVLPEE22 pKa = 4.58 NLLSNEE28 pKa = 4.07 SLSPDD33 pKa = 3.38 TEE35 pKa = 4.33 GQAEE39 pKa = 4.3 EE40 pKa = 5.04 VEE42 pKa = 4.29 QAPYY46 pKa = 9.96 TVDD49 pKa = 3.65 TSCNSCGARR58 pKa = 11.84 VRR60 pKa = 11.84 ICVIATRR67 pKa = 11.84 IAILTLQEE75 pKa = 4.54 LLTLEE80 pKa = 5.33 LNLLCPPCSRR90 pKa = 11.84 TVVRR94 pKa = 11.84 HH95 pKa = 4.57 GRR97 pKa = 11.84 RR98 pKa = 3.23
Molecular weight: 10.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.504
IPC2_protein 4.584
IPC_protein 4.418
Toseland 4.279
ProMoST 4.52
Dawson 4.355
Bjellqvist 4.507
Wikipedia 4.215
Rodwell 4.266
Grimsley 4.19
Solomon 4.355
Lehninger 4.304
Nozaki 4.469
DTASelect 4.584
Thurlkill 4.279
EMBOSS 4.24
Sillero 4.533
Patrickios 3.567
IPC_peptide 4.355
IPC2_peptide 4.52
IPC2.peptide.svr19 4.468
Protein with the highest isoelectric point:
>tr|A0A2D2AM35|A0A2D2AM35_9PAPI Replication protein E1 OS=Gammapapillomavirus 22 OX=1961679 GN=E1 PE=3 SV=1
MM1 pKa = 7.0 NQADD5 pKa = 3.78 LTDD8 pKa = 4.84 RR9 pKa = 11.84 YY10 pKa = 10.82 DD11 pKa = 3.71 ALQEE15 pKa = 4.08 RR16 pKa = 11.84 LMSLYY21 pKa = 10.3 EE22 pKa = 3.95 EE23 pKa = 4.38 SSNTIEE29 pKa = 4.01 AQIMLWEE36 pKa = 4.93 TIRR39 pKa = 11.84 KK40 pKa = 7.83 EE41 pKa = 4.22 QVLLYY46 pKa = 9.47 YY47 pKa = 10.39 GRR49 pKa = 11.84 KK50 pKa = 9.15 EE51 pKa = 4.0 GYY53 pKa = 10.09 KK54 pKa = 10.54 NFGLQPIPNLAVSEE68 pKa = 4.21 YY69 pKa = 9.39 RR70 pKa = 11.84 AKK72 pKa = 10.67 EE73 pKa = 4.1 AIQQVLLLKK82 pKa = 10.41 SLQKK86 pKa = 10.5 SAFGRR91 pKa = 11.84 EE92 pKa = 3.9 EE93 pKa = 3.64 WTLTDD98 pKa = 3.54 TSAEE102 pKa = 4.18 LTHH105 pKa = 6.14 TAPRR109 pKa = 11.84 NTFKK113 pKa = 10.96 KK114 pKa = 10.6 GPFTVNVYY122 pKa = 10.44 FDD124 pKa = 4.54 HH125 pKa = 6.76 NANNVFPYY133 pKa = 8.29 TQWNWLYY140 pKa = 11.11 VQDD143 pKa = 5.76 DD144 pKa = 3.32 NDD146 pKa = 3.35 MWYY149 pKa = 7.88 KK150 pKa = 10.13 TPGLVDD156 pKa = 3.13 INGLYY161 pKa = 10.6 FEE163 pKa = 5.85 DD164 pKa = 4.3 NSGEE168 pKa = 4.08 KK169 pKa = 10.57 NYY171 pKa = 10.46 FLLFATDD178 pKa = 3.25 AEE180 pKa = 5.03 TYY182 pKa = 7.43 GTSGGWTVKK191 pKa = 10.52 YY192 pKa = 10.51 KK193 pKa = 11.08 NEE195 pKa = 4.44 TISTSTPVTSSQQRR209 pKa = 11.84 SFSDD213 pKa = 3.39 SFEE216 pKa = 4.36 GSSKK220 pKa = 10.96 GSVSSSGDD228 pKa = 3.33 AVPLPKK234 pKa = 9.47 TARR237 pKa = 11.84 RR238 pKa = 11.84 KK239 pKa = 7.23 EE240 pKa = 4.32 TEE242 pKa = 3.56 EE243 pKa = 4.06 GRR245 pKa = 11.84 PHH247 pKa = 6.02 STTPTTFTVRR257 pKa = 11.84 RR258 pKa = 11.84 RR259 pKa = 11.84 RR260 pKa = 11.84 RR261 pKa = 11.84 RR262 pKa = 11.84 IQQRR266 pKa = 11.84 EE267 pKa = 3.87 RR268 pKa = 11.84 GTTTVSLRR276 pKa = 11.84 SEE278 pKa = 3.8 RR279 pKa = 11.84 RR280 pKa = 11.84 RR281 pKa = 11.84 TQEE284 pKa = 3.52 SQLGVPAGEE293 pKa = 4.48 VGRR296 pKa = 11.84 GHH298 pKa = 6.76 HH299 pKa = 5.95 LVPRR303 pKa = 11.84 TGLKK307 pKa = 10.08 RR308 pKa = 11.84 LEE310 pKa = 4.1 RR311 pKa = 11.84 LEE313 pKa = 5.52 AEE315 pKa = 4.5 AGDD318 pKa = 3.93 PPVILVKK325 pKa = 10.83 GSANSLKK332 pKa = 9.97 CWRR335 pKa = 11.84 NRR337 pKa = 11.84 SYY339 pKa = 10.94 KK340 pKa = 11.01 ANVPCVKK347 pKa = 9.8 MSTVFRR353 pKa = 11.84 WAEE356 pKa = 3.85 CTEE359 pKa = 3.98 NNIGANNRR367 pKa = 11.84 MLIAFKK373 pKa = 10.79 NRR375 pKa = 11.84 AQRR378 pKa = 11.84 EE379 pKa = 4.39 VFLKK383 pKa = 9.47 TVRR386 pKa = 11.84 FPKK389 pKa = 10.34 GVSYY393 pKa = 11.02 CFGHH397 pKa = 7.59 LDD399 pKa = 3.63 SLL401 pKa = 4.38
Molecular weight: 45.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.822
IPC2_protein 8.814
IPC_protein 8.756
Toseland 9.326
ProMoST 9.194
Dawson 9.648
Bjellqvist 9.414
Wikipedia 9.853
Rodwell 9.838
Grimsley 9.736
Solomon 9.692
Lehninger 9.633
Nozaki 9.458
DTASelect 9.37
Thurlkill 9.487
EMBOSS 9.78
Sillero 9.604
Patrickios 4.876
IPC_peptide 9.677
IPC2_peptide 8.068
IPC2.peptide.svr19 7.774
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2436
98
601
348.0
39.51
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.337 ± 0.282
2.545 ± 0.824
6.117 ± 0.527
6.568 ± 0.374
4.475 ± 0.514
4.885 ± 0.505
1.642 ± 0.161
5.09 ± 0.604
5.952 ± 0.989
9.278 ± 1.095
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.519 ± 0.347
5.172 ± 0.328
6.486 ± 0.978
3.941 ± 0.388
5.624 ± 0.714
6.856 ± 0.921
7.266 ± 1.168
6.199 ± 0.957
1.273 ± 0.264
3.777 ± 0.656
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here