Streptococcus satellite phage Javan408

Taxonomy: Viruses; unclassified bacterial viruses

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4D5ZLW7|A0A4D5ZLW7_9VIRU Integrase OS=Streptococcus satellite phage Javan408 OX=2558684 GN=JavanS408_0014 PE=3 SV=1
MM1 pKa = 7.2KK2 pKa = 9.64TLSDD6 pKa = 3.59TPKK9 pKa = 9.14TFTFITTLPNFEE21 pKa = 4.23QAQKK25 pKa = 10.83AGSALIGYY33 pKa = 7.54MIGTYY38 pKa = 7.49EE39 pKa = 4.14QSAIDD44 pKa = 3.19ITYY47 pKa = 9.12TGNGNLKK54 pKa = 10.41AKK56 pKa = 8.25YY57 pKa = 8.93TSDD60 pKa = 3.44EE61 pKa = 4.24DD62 pKa = 3.56LTEE65 pKa = 4.1SFEE68 pKa = 5.63RR69 pKa = 11.84ITNSLNHH76 pKa = 6.12TEE78 pKa = 4.39DD79 pKa = 3.94YY80 pKa = 11.34EE81 pKa = 4.79DD82 pKa = 4.52FEE84 pKa = 6.32DD85 pKa = 5.91YY86 pKa = 11.03EE87 pKa = 6.14DD88 pKa = 5.95DD89 pKa = 5.03DD90 pKa = 4.03MTIKK94 pKa = 10.93GDD96 pKa = 3.69LDD98 pKa = 3.72TYY100 pKa = 9.69TALIGSFNTLGEE112 pKa = 4.17AQAFTEE118 pKa = 4.01NLEE121 pKa = 4.6DD122 pKa = 4.99SLTNGNNFIYY132 pKa = 10.23EE133 pKa = 4.24QLTDD137 pKa = 3.66MVVLYY142 pKa = 8.6VSPQEE147 pKa = 4.25TIANSTIQEE156 pKa = 4.03LQEE159 pKa = 4.57AYY161 pKa = 10.09VKK163 pKa = 10.81HH164 pKa = 5.46EE165 pKa = 4.58AGYY168 pKa = 10.75EE169 pKa = 3.79PDD171 pKa = 3.52TFRR174 pKa = 11.84LSYY177 pKa = 10.89HH178 pKa = 6.44DD179 pKa = 3.81MRR181 pKa = 11.84VSQLQEE187 pKa = 4.05LNKK190 pKa = 9.84DD191 pKa = 3.48TLINDD196 pKa = 4.08IIAYY200 pKa = 9.15EE201 pKa = 4.44LEE203 pKa = 4.08LLDD206 pKa = 3.89YY207 pKa = 11.08ADD209 pKa = 5.15RR210 pKa = 11.84LLSDD214 pKa = 4.19EE215 pKa = 5.14PLPLDD220 pKa = 4.17DD221 pKa = 3.37QHH223 pKa = 8.21GFEE226 pKa = 4.67TIEE229 pKa = 4.04LLGDD233 pKa = 3.61DD234 pKa = 4.78VINLVKK240 pKa = 10.55EE241 pKa = 4.04LDD243 pKa = 3.47TSKK246 pKa = 10.86EE247 pKa = 3.45FDD249 pKa = 4.59GIHH252 pKa = 7.29DD253 pKa = 4.33YY254 pKa = 11.0IIGGG258 pKa = 3.65

Molecular weight:
29.26 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4D5ZLW9|A0A4D5ZLW9_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan408 OX=2558684 GN=JavanS408_0012 PE=4 SV=1
MM1 pKa = 7.83KK2 pKa = 10.07IIEE5 pKa = 4.24YY6 pKa = 9.9KK7 pKa = 10.26KK8 pKa = 10.62KK9 pKa = 10.66NGTTVYY15 pKa = 10.25RR16 pKa = 11.84ANIYY20 pKa = 10.63LGVDD24 pKa = 3.89SITGKK29 pKa = 10.18KK30 pKa = 10.1VKK32 pKa = 9.9TSVTGRR38 pKa = 11.84TKK40 pKa = 10.69KK41 pKa = 9.61EE42 pKa = 3.43VKK44 pKa = 9.82IRR46 pKa = 11.84IKK48 pKa = 10.17EE49 pKa = 3.89AQHH52 pKa = 5.62NFKK55 pKa = 11.25ANGQTVTKK63 pKa = 10.04AVPVEE68 pKa = 4.14NYY70 pKa = 10.2KK71 pKa = 10.55EE72 pKa = 4.15LAEE75 pKa = 4.41LWLKK79 pKa = 10.56SYY81 pKa = 11.07EE82 pKa = 4.18MTVKK86 pKa = 10.03PQTFIATKK94 pKa = 10.5RR95 pKa = 11.84MLHH98 pKa = 5.09NHH100 pKa = 6.46LMPVFGDD107 pKa = 3.29IKK109 pKa = 10.62LDD111 pKa = 3.65KK112 pKa = 10.92LSVSYY117 pKa = 10.66IQGFINDD124 pKa = 3.72LSKK127 pKa = 11.25EE128 pKa = 4.13FVHH131 pKa = 6.97FGTVHH136 pKa = 6.64SINKK140 pKa = 9.37RR141 pKa = 11.84VLQYY145 pKa = 10.92GVSLQLIPFNPARR158 pKa = 11.84DD159 pKa = 3.69VILPKK164 pKa = 10.31VVKK167 pKa = 10.11PEE169 pKa = 3.58NKK171 pKa = 9.58AIKK174 pKa = 10.21FIDD177 pKa = 3.82YY178 pKa = 10.83NDD180 pKa = 3.82LKK182 pKa = 11.48ALMSYY187 pKa = 8.12MEE189 pKa = 4.43KK190 pKa = 10.66LSNKK194 pKa = 9.25KK195 pKa = 9.16YY196 pKa = 10.55SYY198 pKa = 10.49YY199 pKa = 10.32FDD201 pKa = 3.7YY202 pKa = 11.14VLYY205 pKa = 10.73SILLATGCRR214 pKa = 11.84FGEE217 pKa = 4.33AVALTWSDD225 pKa = 2.68IDD227 pKa = 4.54FEE229 pKa = 4.55NATIDD234 pKa = 3.43INKK237 pKa = 9.88NYY239 pKa = 9.76NRR241 pKa = 11.84LVDD244 pKa = 3.54IVGTPKK250 pKa = 10.52SKK252 pKa = 10.62AGYY255 pKa = 9.05RR256 pKa = 11.84VISIDD261 pKa = 3.3QKK263 pKa = 9.93TINLLRR269 pKa = 11.84LYY271 pKa = 10.67KK272 pKa = 10.3NRR274 pKa = 11.84QRR276 pKa = 11.84QLFSEE281 pKa = 4.82VSSEE285 pKa = 4.17VPSVVFATPTRR296 pKa = 11.84KK297 pKa = 9.52YY298 pKa = 9.69QNTAIRR304 pKa = 11.84QGSLDD309 pKa = 3.54RR310 pKa = 11.84RR311 pKa = 11.84LKK313 pKa = 10.74EE314 pKa = 3.58IGCPRR319 pKa = 11.84FTFHH323 pKa = 7.93AFRR326 pKa = 11.84HH327 pKa = 4.69THH329 pKa = 6.92ASLLLNAGISYY340 pKa = 10.68KK341 pKa = 10.23EE342 pKa = 3.61LQYY345 pKa = 11.49RR346 pKa = 11.84LGHH349 pKa = 6.19ATLAMTMDD357 pKa = 4.24IYY359 pKa = 11.79SHH361 pKa = 6.95LSKK364 pKa = 10.92DD365 pKa = 3.51KK366 pKa = 10.41EE367 pKa = 4.25KK368 pKa = 10.93EE369 pKa = 3.85AVSYY373 pKa = 9.5YY374 pKa = 10.62EE375 pKa = 4.12KK376 pKa = 10.7AINSLL381 pKa = 3.57

Molecular weight:
43.84 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2523

42

524

180.2

20.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.113 ± 0.403

0.713 ± 0.156

6.143 ± 0.681

7.293 ± 0.523

3.686 ± 0.385

4.479 ± 0.3

1.546 ± 0.246

8.522 ± 0.621

9.116 ± 0.735

9.631 ± 0.474

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.236

6.659 ± 0.491

3.448 ± 0.426

4.082 ± 0.402

4.4 ± 0.483

5.153 ± 0.421

7.095 ± 0.596

4.36 ± 0.626

0.674 ± 0.174

5.509 ± 0.254

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski