Streptococcus satellite phage Javan408
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D5ZLW7|A0A4D5ZLW7_9VIRU Integrase OS=Streptococcus satellite phage Javan408 OX=2558684 GN=JavanS408_0014 PE=3 SV=1
MM1 pKa = 7.2 KK2 pKa = 9.64 TLSDD6 pKa = 3.59 TPKK9 pKa = 9.14 TFTFITTLPNFEE21 pKa = 4.23 QAQKK25 pKa = 10.83 AGSALIGYY33 pKa = 7.54 MIGTYY38 pKa = 7.49 EE39 pKa = 4.14 QSAIDD44 pKa = 3.19 ITYY47 pKa = 9.12 TGNGNLKK54 pKa = 10.41 AKK56 pKa = 8.25 YY57 pKa = 8.93 TSDD60 pKa = 3.44 EE61 pKa = 4.24 DD62 pKa = 3.56 LTEE65 pKa = 4.1 SFEE68 pKa = 5.63 RR69 pKa = 11.84 ITNSLNHH76 pKa = 6.12 TEE78 pKa = 4.39 DD79 pKa = 3.94 YY80 pKa = 11.34 EE81 pKa = 4.79 DD82 pKa = 4.52 FEE84 pKa = 6.32 DD85 pKa = 5.91 YY86 pKa = 11.03 EE87 pKa = 6.14 DD88 pKa = 5.95 DD89 pKa = 5.03 DD90 pKa = 4.03 MTIKK94 pKa = 10.93 GDD96 pKa = 3.69 LDD98 pKa = 3.72 TYY100 pKa = 9.69 TALIGSFNTLGEE112 pKa = 4.17 AQAFTEE118 pKa = 4.01 NLEE121 pKa = 4.6 DD122 pKa = 4.99 SLTNGNNFIYY132 pKa = 10.23 EE133 pKa = 4.24 QLTDD137 pKa = 3.66 MVVLYY142 pKa = 8.6 VSPQEE147 pKa = 4.25 TIANSTIQEE156 pKa = 4.03 LQEE159 pKa = 4.57 AYY161 pKa = 10.09 VKK163 pKa = 10.81 HH164 pKa = 5.46 EE165 pKa = 4.58 AGYY168 pKa = 10.75 EE169 pKa = 3.79 PDD171 pKa = 3.52 TFRR174 pKa = 11.84 LSYY177 pKa = 10.89 HH178 pKa = 6.44 DD179 pKa = 3.81 MRR181 pKa = 11.84 VSQLQEE187 pKa = 4.05 LNKK190 pKa = 9.84 DD191 pKa = 3.48 TLINDD196 pKa = 4.08 IIAYY200 pKa = 9.15 EE201 pKa = 4.44 LEE203 pKa = 4.08 LLDD206 pKa = 3.89 YY207 pKa = 11.08 ADD209 pKa = 5.15 RR210 pKa = 11.84 LLSDD214 pKa = 4.19 EE215 pKa = 5.14 PLPLDD220 pKa = 4.17 DD221 pKa = 3.37 QHH223 pKa = 8.21 GFEE226 pKa = 4.67 TIEE229 pKa = 4.04 LLGDD233 pKa = 3.61 DD234 pKa = 4.78 VINLVKK240 pKa = 10.55 EE241 pKa = 4.04 LDD243 pKa = 3.47 TSKK246 pKa = 10.86 EE247 pKa = 3.45 FDD249 pKa = 4.59 GIHH252 pKa = 7.29 DD253 pKa = 4.33 YY254 pKa = 11.0 IIGGG258 pKa = 3.65
Molecular weight: 29.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.74
IPC2_protein 3.834
IPC_protein 3.846
Toseland 3.63
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.732
Rodwell 3.668
Grimsley 3.541
Solomon 3.808
Lehninger 3.77
Nozaki 3.923
DTASelect 4.139
Thurlkill 3.668
EMBOSS 3.745
Sillero 3.961
Patrickios 1.875
IPC_peptide 3.808
IPC2_peptide 3.935
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|A0A4D5ZLW9|A0A4D5ZLW9_9VIRU Putative transcriptional regulator OS=Streptococcus satellite phage Javan408 OX=2558684 GN=JavanS408_0012 PE=4 SV=1
MM1 pKa = 7.83 KK2 pKa = 10.07 IIEE5 pKa = 4.24 YY6 pKa = 9.9 KK7 pKa = 10.26 KK8 pKa = 10.62 KK9 pKa = 10.66 NGTTVYY15 pKa = 10.25 RR16 pKa = 11.84 ANIYY20 pKa = 10.63 LGVDD24 pKa = 3.89 SITGKK29 pKa = 10.18 KK30 pKa = 10.1 VKK32 pKa = 9.9 TSVTGRR38 pKa = 11.84 TKK40 pKa = 10.69 KK41 pKa = 9.61 EE42 pKa = 3.43 VKK44 pKa = 9.82 IRR46 pKa = 11.84 IKK48 pKa = 10.17 EE49 pKa = 3.89 AQHH52 pKa = 5.62 NFKK55 pKa = 11.25 ANGQTVTKK63 pKa = 10.04 AVPVEE68 pKa = 4.14 NYY70 pKa = 10.2 KK71 pKa = 10.55 EE72 pKa = 4.15 LAEE75 pKa = 4.41 LWLKK79 pKa = 10.56 SYY81 pKa = 11.07 EE82 pKa = 4.18 MTVKK86 pKa = 10.03 PQTFIATKK94 pKa = 10.5 RR95 pKa = 11.84 MLHH98 pKa = 5.09 NHH100 pKa = 6.46 LMPVFGDD107 pKa = 3.29 IKK109 pKa = 10.62 LDD111 pKa = 3.65 KK112 pKa = 10.92 LSVSYY117 pKa = 10.66 IQGFINDD124 pKa = 3.72 LSKK127 pKa = 11.25 EE128 pKa = 4.13 FVHH131 pKa = 6.97 FGTVHH136 pKa = 6.64 SINKK140 pKa = 9.37 RR141 pKa = 11.84 VLQYY145 pKa = 10.92 GVSLQLIPFNPARR158 pKa = 11.84 DD159 pKa = 3.69 VILPKK164 pKa = 10.31 VVKK167 pKa = 10.11 PEE169 pKa = 3.58 NKK171 pKa = 9.58 AIKK174 pKa = 10.21 FIDD177 pKa = 3.82 YY178 pKa = 10.83 NDD180 pKa = 3.82 LKK182 pKa = 11.48 ALMSYY187 pKa = 8.12 MEE189 pKa = 4.43 KK190 pKa = 10.66 LSNKK194 pKa = 9.25 KK195 pKa = 9.16 YY196 pKa = 10.55 SYY198 pKa = 10.49 YY199 pKa = 10.32 FDD201 pKa = 3.7 YY202 pKa = 11.14 VLYY205 pKa = 10.73 SILLATGCRR214 pKa = 11.84 FGEE217 pKa = 4.33 AVALTWSDD225 pKa = 2.68 IDD227 pKa = 4.54 FEE229 pKa = 4.55 NATIDD234 pKa = 3.43 INKK237 pKa = 9.88 NYY239 pKa = 9.76 NRR241 pKa = 11.84 LVDD244 pKa = 3.54 IVGTPKK250 pKa = 10.52 SKK252 pKa = 10.62 AGYY255 pKa = 9.05 RR256 pKa = 11.84 VISIDD261 pKa = 3.3 QKK263 pKa = 9.93 TINLLRR269 pKa = 11.84 LYY271 pKa = 10.67 KK272 pKa = 10.3 NRR274 pKa = 11.84 QRR276 pKa = 11.84 QLFSEE281 pKa = 4.82 VSSEE285 pKa = 4.17 VPSVVFATPTRR296 pKa = 11.84 KK297 pKa = 9.52 YY298 pKa = 9.69 QNTAIRR304 pKa = 11.84 QGSLDD309 pKa = 3.54 RR310 pKa = 11.84 RR311 pKa = 11.84 LKK313 pKa = 10.74 EE314 pKa = 3.58 IGCPRR319 pKa = 11.84 FTFHH323 pKa = 7.93 AFRR326 pKa = 11.84 HH327 pKa = 4.69 THH329 pKa = 6.92 ASLLLNAGISYY340 pKa = 10.68 KK341 pKa = 10.23 EE342 pKa = 3.61 LQYY345 pKa = 11.49 RR346 pKa = 11.84 LGHH349 pKa = 6.19 ATLAMTMDD357 pKa = 4.24 IYY359 pKa = 11.79 SHH361 pKa = 6.95 LSKK364 pKa = 10.92 DD365 pKa = 3.51 KK366 pKa = 10.41 EE367 pKa = 4.25 KK368 pKa = 10.93 EE369 pKa = 3.85 AVSYY373 pKa = 9.5 YY374 pKa = 10.62 EE375 pKa = 4.12 KK376 pKa = 10.7 AINSLL381 pKa = 3.57
Molecular weight: 43.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.12
IPC2_protein 9.238
IPC_protein 9.121
Toseland 9.823
ProMoST 9.531
Dawson 10.058
Bjellqvist 9.721
Wikipedia 10.233
Rodwell 10.496
Grimsley 10.131
Solomon 10.072
Lehninger 10.028
Nozaki 9.809
DTASelect 9.721
Thurlkill 9.897
EMBOSS 10.233
Sillero 9.97
Patrickios 7.585
IPC_peptide 10.072
IPC2_peptide 8.229
IPC2.peptide.svr19 8.322
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
14
0
14
2523
42
524
180.2
20.85
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.113 ± 0.403
0.713 ± 0.156
6.143 ± 0.681
7.293 ± 0.523
3.686 ± 0.385
4.479 ± 0.3
1.546 ± 0.246
8.522 ± 0.621
9.116 ± 0.735
9.631 ± 0.474
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.378 ± 0.236
6.659 ± 0.491
3.448 ± 0.426
4.082 ± 0.402
4.4 ± 0.483
5.153 ± 0.421
7.095 ± 0.596
4.36 ± 0.626
0.674 ± 0.174
5.509 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here