Escherichia phage vB_EcoM-Ro157c2YLVW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Punavirus; unclassified Punavirus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 86 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A499QY17|A0A499QY17_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM-Ro157c2YLVW OX=2178932 GN=vBEcoMRo157c2YLVW_00079 PE=4 SV=1
MM1 pKa = 7.67AGFFDD6 pKa = 6.35DD7 pKa = 4.02MFEE10 pKa = 4.23DD11 pKa = 4.51TEE13 pKa = 4.35PSQQVTGDD21 pKa = 3.88NLPDD25 pKa = 3.78TEE27 pKa = 5.12SDD29 pKa = 3.19PDD31 pKa = 3.55IPGEE35 pKa = 3.96GSEE38 pKa = 4.92LIEE41 pKa = 5.0EE42 pKa = 4.16EE43 pKa = 5.62DD44 pKa = 2.98IDD46 pKa = 5.84AEE48 pKa = 4.4IEE50 pKa = 4.07TDD52 pKa = 3.67GVNVGNIVDD61 pKa = 3.98PVEE64 pKa = 5.38DD65 pKa = 3.36NHH67 pKa = 7.56LPNLDD72 pKa = 4.1HH73 pKa = 7.25GLLSDD78 pKa = 3.05SGVRR82 pKa = 11.84HH83 pKa = 6.8RR84 pKa = 11.84YY85 pKa = 8.53QGHH88 pKa = 6.16AVFNNLVRR96 pKa = 11.84MDD98 pKa = 3.44WLKK101 pKa = 10.95AIKK104 pKa = 10.09LDD106 pKa = 3.98PDD108 pKa = 3.48SFDD111 pKa = 3.23AVLYY115 pKa = 10.32RR116 pKa = 11.84AIPYY120 pKa = 9.7RR121 pKa = 11.84NKK123 pKa = 10.2NAPEE127 pKa = 3.87TAPEE131 pKa = 4.35IIEE134 pKa = 4.19PNQRR138 pKa = 11.84IYY140 pKa = 10.97DD141 pKa = 3.88YY142 pKa = 11.01QDD144 pKa = 3.82PEE146 pKa = 5.45LITALDD152 pKa = 4.61CPDD155 pKa = 3.92EE156 pKa = 4.12MDD158 pKa = 4.85AFYY161 pKa = 11.35ALYY164 pKa = 10.55DD165 pKa = 3.88GSDD168 pKa = 3.32NTGISDD174 pKa = 3.48SALILRR180 pKa = 11.84LAAVNVPVGSMLEE193 pKa = 4.01WLEE196 pKa = 3.88QLSDD200 pKa = 3.08GTTIRR205 pKa = 11.84RR206 pKa = 11.84FWYY209 pKa = 7.82IHH211 pKa = 6.68KK212 pKa = 9.88IFNYY216 pKa = 7.45GTARR220 pKa = 11.84VGSLFYY226 pKa = 10.43CVPSRR231 pKa = 11.84AFEE234 pKa = 4.35GNFIGDD240 pKa = 3.71SEE242 pKa = 4.34

Molecular weight: 27.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A499R1D0|A0A499R1D0_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoM-Ro157c2YLVW OX=2178932 GN=vBEcoMRo157c2YLVW_00088 PE=4 SV=1
MM1 pKa = 7.11WPFRR5 pKa = 11.84RR6 pKa = 11.84KK7 pKa = 7.61YY8 pKa = 10.16HH9 pKa = 5.18YY10 pKa = 9.76WLIAFVTPTGGIRR23 pKa = 11.84HH24 pKa = 6.4VITRR28 pKa = 11.84YY29 pKa = 8.82RR30 pKa = 11.84NKK32 pKa = 10.34RR33 pKa = 11.84LTLARR38 pKa = 11.84ILQAAIGEE46 pKa = 4.42GLDD49 pKa = 3.78TNCVVLPPSYY59 pKa = 9.98LGKK62 pKa = 8.44MTEE65 pKa = 3.99AQANTEE71 pKa = 4.1LL72 pKa = 4.23

Molecular weight: 8.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

86

0

86

23705

42

2255

275.6

30.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.458 ± 0.31

0.996 ± 0.126

6.096 ± 0.217

6.682 ± 0.226

3.759 ± 0.164

6.859 ± 0.271

1.738 ± 0.121

5.931 ± 0.155

5.969 ± 0.259

8.656 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.556 ± 0.13

4.712 ± 0.147

3.987 ± 0.149

3.957 ± 0.152

5.488 ± 0.202

6.551 ± 0.174

6.011 ± 0.302

6.332 ± 0.173

1.228 ± 0.084

3.033 ± 0.145
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski