CRESS virus sp. ctrF513

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; unclassified Cressdnaviricota; CRESS viruses

Average proteome isoelectric point is 8.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q2WAE2|A0A5Q2WAE2_9VIRU Rep protein OS=CRESS virus sp. ctrF513 OX=2656690 PE=4 SV=1
MM1 pKa = 8.0DD2 pKa = 4.74NSSGNTKK9 pKa = 9.61PRR11 pKa = 11.84NIKK14 pKa = 9.48KK15 pKa = 9.3LHH17 pKa = 5.81WLLTINNPKK26 pKa = 10.23EE27 pKa = 4.06NEE29 pKa = 4.03LEE31 pKa = 4.07EE32 pKa = 3.91LAKK35 pKa = 10.04IYY37 pKa = 10.37KK38 pKa = 8.44ITGQYY43 pKa = 10.16EE44 pKa = 3.8KK45 pKa = 11.13GEE47 pKa = 4.05NGTRR51 pKa = 11.84HH52 pKa = 5.21IQCVVSFNCQRR63 pKa = 11.84YY64 pKa = 8.14FNSIKK69 pKa = 10.68EE70 pKa = 4.15MFPRR74 pKa = 11.84AHH76 pKa = 6.78IEE78 pKa = 3.5PVRR81 pKa = 11.84RR82 pKa = 11.84INDD85 pKa = 3.16AVNYY89 pKa = 5.48VTKK92 pKa = 10.14IDD94 pKa = 3.69TRR96 pKa = 11.84DD97 pKa = 3.55CVSPVIHH104 pKa = 7.57PDD106 pKa = 2.48HH107 pKa = 6.55KK108 pKa = 10.77RR109 pKa = 11.84YY110 pKa = 9.25IKK112 pKa = 10.69RR113 pKa = 11.84EE114 pKa = 3.89IKK116 pKa = 10.68NKK118 pKa = 9.94FLMCKK123 pKa = 8.87PYY125 pKa = 10.18KK126 pKa = 8.27WQEE129 pKa = 3.91EE130 pKa = 4.35IMTILDD136 pKa = 4.09KK137 pKa = 11.38EE138 pKa = 4.28PDD140 pKa = 3.36DD141 pKa = 4.45RR142 pKa = 11.84TIYY145 pKa = 9.82WYY147 pKa = 9.22WDD149 pKa = 3.87PIGCTGKK156 pKa = 10.51SVFTKK161 pKa = 10.05HH162 pKa = 6.58LVMTRR167 pKa = 11.84GAFAVGGRR175 pKa = 11.84VQDD178 pKa = 3.8ALYY181 pKa = 9.75MIMKK185 pKa = 8.87KK186 pKa = 8.39QKK188 pKa = 10.13KK189 pKa = 9.38GLEE192 pKa = 3.72PDD194 pKa = 4.01IIIFDD199 pKa = 3.75IPRR202 pKa = 11.84NQGNDD207 pKa = 2.79VSYY210 pKa = 11.32NAIEE214 pKa = 4.41SIKK217 pKa = 10.73NGCCFSSKK225 pKa = 10.66YY226 pKa = 9.96KK227 pKa = 10.94SKK229 pKa = 11.1DD230 pKa = 3.34LIFNTPHH237 pKa = 7.77IIIFANSEE245 pKa = 4.23PNLMNLSSDD254 pKa = 3.58RR255 pKa = 11.84WCVRR259 pKa = 11.84KK260 pKa = 9.8IEE262 pKa = 4.15LTYY265 pKa = 10.91LPNLVQDD272 pKa = 4.34INDD275 pKa = 3.91KK276 pKa = 11.31YY277 pKa = 11.57NLL279 pKa = 3.73

Molecular weight: 32.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q2WAE2|A0A5Q2WAE2_9VIRU Rep protein OS=CRESS virus sp. ctrF513 OX=2656690 PE=4 SV=1
MM1 pKa = 7.44SYY3 pKa = 10.43KK4 pKa = 10.08RR5 pKa = 11.84RR6 pKa = 11.84YY7 pKa = 7.86KK8 pKa = 10.35KK9 pKa = 9.98KK10 pKa = 8.38RR11 pKa = 11.84SRR13 pKa = 11.84FGRR16 pKa = 11.84FLRR19 pKa = 11.84GAAKK23 pKa = 8.87TAMVASTALRR33 pKa = 11.84VAKK36 pKa = 9.35QVKK39 pKa = 9.19GLVNVEE45 pKa = 3.86YY46 pKa = 10.58KK47 pKa = 10.59KK48 pKa = 10.69RR49 pKa = 11.84DD50 pKa = 3.43LAQSEE55 pKa = 4.74SPDD58 pKa = 3.47NATLSVAGLCSIGQGDD74 pKa = 3.72TDD76 pKa = 3.43QARR79 pKa = 11.84NGNEE83 pKa = 3.39VRR85 pKa = 11.84AKK87 pKa = 10.5GIQFRR92 pKa = 11.84LSLSKK97 pKa = 10.79HH98 pKa = 4.08STATNTMVRR107 pKa = 11.84IMLLRR112 pKa = 11.84CVDD115 pKa = 3.51GGTPTVNILEE125 pKa = 4.23NVGTYY130 pKa = 10.23RR131 pKa = 11.84CLAPKK136 pKa = 10.29ASQYY140 pKa = 11.55ANQFITIKK148 pKa = 9.99EE149 pKa = 4.06WTVVLNQTTPIKK161 pKa = 10.28IINYY165 pKa = 7.3YY166 pKa = 10.4KK167 pKa = 10.74KK168 pKa = 10.5LNHH171 pKa = 6.43HH172 pKa = 6.66IKK174 pKa = 9.67WYY176 pKa = 8.72STGSTDD182 pKa = 4.09FKK184 pKa = 11.16SGSYY188 pKa = 9.78CLAMLSNEE196 pKa = 3.8ATYY199 pKa = 10.8LPNIIGYY206 pKa = 8.73SRR208 pKa = 11.84IRR210 pKa = 11.84FIDD213 pKa = 3.25NN214 pKa = 3.05

Molecular weight: 24.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

493

214

279

246.5

28.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.071 ± 1.717

2.434 ± 0.338

4.868 ± 0.955

4.462 ± 0.992

3.245 ± 0.264

5.274 ± 0.758

2.028 ± 0.375

8.722 ± 1.303

9.331 ± 0.55

6.897 ± 0.626

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.637 ± 0.18

7.505 ± 0.576

3.854 ± 0.907

3.245 ± 0.295

6.288 ± 0.711

6.288 ± 0.99

6.085 ± 1.111

5.477 ± 0.358

1.42 ± 0.29

4.868 ± 0.163
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski