Flavobacterium phage vB_FspP_elemoD_13-5B

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 91 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7IPM6|A0A7D7IPM6_9CAUD Uncharacterized protein OS=Flavobacterium phage vB_FspP_elemoD_13-5B OX=2743801 GN=elemo135B_phanotate56 PE=4 SV=1
MM1 pKa = 7.79TDD3 pKa = 3.31LSEE6 pKa = 5.18DD7 pKa = 3.58DD8 pKa = 4.24LYY10 pKa = 11.43EE11 pKa = 4.44VIMPIVNQEE20 pKa = 3.69RR21 pKa = 11.84YY22 pKa = 10.33GYY24 pKa = 10.29EE25 pKa = 4.3DD26 pKa = 3.96YY27 pKa = 11.48DD28 pKa = 4.21NEE30 pKa = 4.57SLLLALKK37 pKa = 10.23KK38 pKa = 10.26RR39 pKa = 11.84YY40 pKa = 6.91PTNKK44 pKa = 9.04VDD46 pKa = 3.7IIEE49 pKa = 4.27IEE51 pKa = 4.33EE52 pKa = 3.65ISYY55 pKa = 11.16

Molecular weight: 6.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7F786|A0A7D7F786_9CAUD 5'-3' exonuclease OS=Flavobacterium phage vB_FspP_elemoD_13-5B OX=2743801 GN=elemo135B_phanotate51 PE=4 SV=1
MM1 pKa = 7.8KK2 pKa = 10.28GRR4 pKa = 11.84SNSSSRR10 pKa = 11.84RR11 pKa = 11.84LSLTISTSSTMNDD24 pKa = 4.57FINKK28 pKa = 9.34KK29 pKa = 10.49LGVNKK34 pKa = 10.2DD35 pKa = 3.25NKK37 pKa = 10.46RR38 pKa = 11.84PILIKK43 pKa = 9.07PTKK46 pKa = 9.74KK47 pKa = 10.5

Molecular weight: 5.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

91

0

91

19251

30

3757

211.5

24.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.246 ± 0.389

0.717 ± 0.141

6.862 ± 0.233

7.47 ± 0.298

4.576 ± 0.212

5.87 ± 0.266

1.169 ± 0.163

7.714 ± 0.257

9.314 ± 0.628

7.839 ± 0.284

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.196

7.215 ± 0.209

2.691 ± 0.135

3.039 ± 0.234

3.47 ± 0.178

7.246 ± 0.243

5.75 ± 0.259

6.057 ± 0.213

0.914 ± 0.102

4.602 ± 0.344
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski