Amino acid dipepetide frequency for Terrabacter sp. Root85

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.944AlaAla: 19.944 ± 0.17	0.939AlaCys: 0.939 ± 0.028	8.329AlaAsp: 8.329 ± 0.093	7.065AlaGlu: 7.065 ± 0.079	3.51AlaPhe: 3.51 ± 0.051	12.269AlaGly: 12.269 ± 0.092	2.728AlaHis: 2.728 ± 0.041	4.306AlaIle: 4.306 ± 0.064	2.707AlaLys: 2.707 ± 0.066	13.682AlaLeu: 13.682 ± 0.133	2.723AlaMet: 2.723 ± 0.051	2.038AlaAsn: 2.038 ± 0.049	6.592AlaPro: 6.592 ± 0.085	3.506AlaGln: 3.506 ± 0.053	9.631AlaArg: 9.631 ± 0.091	7.076AlaSer: 7.076 ± 0.079	8.327AlaThr: 8.327 ± 0.087	12.256AlaVal: 12.256 ± 0.12	1.973AlaTrp: 1.973 ± 0.035	2.623AlaTyr: 2.623 ± 0.049	0.001AlaXaa: 0.001 ± 0.001
Cys	0.804CysAla: 0.804 ± 0.026	0.066CysCys: 0.066 ± 0.008	0.418CysAsp: 0.418 ± 0.018	0.328CysGlu: 0.328 ± 0.015	0.207CysPhe: 0.207 ± 0.014	0.774CysGly: 0.774 ± 0.028	0.18CysHis: 0.18 ± 0.011	0.223CysIle: 0.223 ± 0.014	0.089CysLys: 0.089 ± 0.009	0.62CysLeu: 0.62 ± 0.021	0.108CysMet: 0.108 ± 0.008	0.126CysAsn: 0.126 ± 0.01	0.4CysPro: 0.4 ± 0.019	0.146CysGln: 0.146 ± 0.01	0.503CysArg: 0.503 ± 0.02	0.404CysSer: 0.404 ± 0.017	0.458CysThr: 0.458 ± 0.022	0.607CysVal: 0.607 ± 0.02	0.099CysTrp: 0.099 ± 0.009	0.125CysTyr: 0.125 ± 0.009	0.0CysXaa: 0.0 ± 0.0
Asp	8.433AspAla: 8.433 ± 0.084	0.387AspCys: 0.387 ± 0.015	4.692AspAsp: 4.692 ± 0.069	4.333AspGlu: 4.333 ± 0.061	1.61AspPhe: 1.61 ± 0.037	6.533AspGly: 6.533 ± 0.073	1.623AspHis: 1.623 ± 0.036	1.96AspIle: 1.96 ± 0.039	1.127AspLys: 1.127 ± 0.033	7.039AspLeu: 7.039 ± 0.075	0.806AspMet: 0.806 ± 0.027	0.913AspAsn: 0.913 ± 0.031	4.584AspPro: 4.584 ± 0.064	1.571AspGln: 1.571 ± 0.035	4.855AspArg: 4.855 ± 0.058	2.466AspSer: 2.466 ± 0.044	3.141AspThr: 3.141 ± 0.044	6.533AspVal: 6.533 ± 0.076	1.032AspTrp: 1.032 ± 0.025	1.176AspTyr: 1.176 ± 0.03	0.0AspXaa: 0.0 ± 0.0
Glu	6.99GluAla: 6.99 ± 0.082	0.325GluCys: 0.325 ± 0.014	2.628GluAsp: 2.628 ± 0.044	2.658GluGlu: 2.658 ± 0.053	1.302GluPhe: 1.302 ± 0.031	4.188GluGly: 4.188 ± 0.057	1.663GluHis: 1.663 ± 0.038	2.101GluIle: 2.101 ± 0.039	1.14GluLys: 1.14 ± 0.031	5.791GluLeu: 5.791 ± 0.067	0.888GluMet: 0.888 ± 0.024	0.813GluAsn: 0.813 ± 0.025	3.122GluPro: 3.122 ± 0.055	2.145GluGln: 2.145 ± 0.045	5.163GluArg: 5.163 ± 0.081	2.86GluSer: 2.86 ± 0.039	2.781GluThr: 2.781 ± 0.039	4.923GluVal: 4.923 ± 0.069	0.79GluTrp: 0.79 ± 0.023	0.823GluTyr: 0.823 ± 0.022	0.0GluXaa: 0.0 ± 0.0
Phe	3.52PheAla: 3.52 ± 0.049	0.226PheCys: 0.226 ± 0.012	2.055PheAsp: 2.055 ± 0.038	1.534PheGlu: 1.534 ± 0.036	0.908PhePhe: 0.908 ± 0.03	2.974PheGly: 2.974 ± 0.054	0.58PheHis: 0.58 ± 0.022	0.869PheIle: 0.869 ± 0.028	0.466PheLys: 0.466 ± 0.02	2.368PheLeu: 2.368 ± 0.046	0.422PheMet: 0.422 ± 0.02	0.579PheAsn: 0.579 ± 0.021	1.252PhePro: 1.252 ± 0.03	0.579PheGln: 0.579 ± 0.02	1.612PheArg: 1.612 ± 0.034	1.494PheSer: 1.494 ± 0.034	1.937PheThr: 1.937 ± 0.039	2.896PheVal: 2.896 ± 0.048	0.443PheTrp: 0.443 ± 0.02	0.617PheTyr: 0.617 ± 0.022	0.0PheXaa: 0.0 ± 0.0
Gly	10.886GlyAla: 10.886 ± 0.108	0.671GlyCys: 0.671 ± 0.021	5.678GlyAsp: 5.678 ± 0.072	4.491GlyGlu: 4.491 ± 0.063	2.884GlyPhe: 2.884 ± 0.051	8.241GlyGly: 8.241 ± 0.111	2.333GlyHis: 2.333 ± 0.039	3.81GlyIle: 3.81 ± 0.05	2.056GlyLys: 2.056 ± 0.052	9.645GlyLeu: 9.645 ± 0.089	1.846GlyMet: 1.846 ± 0.038	1.709GlyAsn: 1.709 ± 0.046	4.667GlyPro: 4.667 ± 0.06	2.689GlyGln: 2.689 ± 0.045	7.276GlyArg: 7.276 ± 0.075	5.881GlySer: 5.881 ± 0.072	6.243GlyThr: 6.243 ± 0.095	8.207GlyVal: 8.207 ± 0.071	1.796GlyTrp: 1.796 ± 0.042	2.081GlyTyr: 2.081 ± 0.043	0.001GlyXaa: 0.001 ± 0.001
His	2.678HisAla: 2.678 ± 0.051	0.167HisCys: 0.167 ± 0.009	1.69HisAsp: 1.69 ± 0.038	1.369HisGlu: 1.369 ± 0.03	0.579HisPhe: 0.579 ± 0.022	2.293HisGly: 2.293 ± 0.04	0.676HisHis: 0.676 ± 0.023	0.549HisIle: 0.549 ± 0.02	0.273HisLys: 0.273 ± 0.012	2.488HisLeu: 2.488 ± 0.047	0.298HisMet: 0.298 ± 0.014	0.356HisAsn: 0.356 ± 0.017	1.635HisPro: 1.635 ± 0.041	0.574HisGln: 0.574 ± 0.02	1.857HisArg: 1.857 ± 0.036	0.929HisSer: 0.929 ± 0.025	1.166HisThr: 1.166 ± 0.026	2.332HisVal: 2.332 ± 0.048	0.342HisTrp: 0.342 ± 0.015	0.43HisTyr: 0.43 ± 0.017	0.001HisXaa: 0.001 ± 0.001
Ile	4.961IleAla: 4.961 ± 0.068	0.273IleCys: 0.273 ± 0.014	2.661IleAsp: 2.661 ± 0.051	2.159IleGlu: 2.159 ± 0.039	0.824IlePhe: 0.824 ± 0.027	3.752IleGly: 3.752 ± 0.059	0.688IleHis: 0.688 ± 0.021	1.147IleIle: 1.147 ± 0.032	0.688IleLys: 0.688 ± 0.024	2.554IleLeu: 2.554 ± 0.053	0.468IleMet: 0.468 ± 0.016	0.697IleAsn: 0.697 ± 0.025	1.763IlePro: 1.763 ± 0.036	0.73IleGln: 0.73 ± 0.021	2.245IleArg: 2.245 ± 0.043	1.806IleSer: 1.806 ± 0.037	2.25IleThr: 2.25 ± 0.044	3.364IleVal: 3.364 ± 0.048	0.419IleTrp: 0.419 ± 0.017	0.58IleTyr: 0.58 ± 0.024	0.0IleXaa: 0.0 ± 0.0
Lys	2.693LysAla: 2.693 ± 0.062	0.086LysCys: 0.086 ± 0.009	1.25LysAsp: 1.25 ± 0.038	0.95LysGlu: 0.95 ± 0.029	0.428LysPhe: 0.428 ± 0.016	1.743LysGly: 1.743 ± 0.039	0.433LysHis: 0.433 ± 0.018	0.779LysIle: 0.779 ± 0.03	0.699LysLys: 0.699 ± 0.032	1.572LysLeu: 1.572 ± 0.042	0.324LysMet: 0.324 ± 0.014	0.456LysAsn: 0.456 ± 0.019	1.137LysPro: 1.137 ± 0.03	0.667LysGln: 0.667 ± 0.026	1.338LysArg: 1.338 ± 0.037	1.061LysSer: 1.061 ± 0.029	1.201LysThr: 1.201 ± 0.037	1.942LysVal: 1.942 ± 0.044	0.231LysTrp: 0.231 ± 0.011	0.393LysTyr: 0.393 ± 0.018	0.0LysXaa: 0.0 ± 0.0
Leu	14.163LeuAla: 14.163 ± 0.111	0.637LeuCys: 0.637 ± 0.02	7.044LeuAsp: 7.044 ± 0.067	5.118LeuGlu: 5.118 ± 0.068	2.436LeuPhe: 2.436 ± 0.051	9.606LeuGly: 9.606 ± 0.091	2.094LeuHis: 2.094 ± 0.044	2.855LeuIle: 2.855 ± 0.05	1.701LeuLys: 1.701 ± 0.041	10.088LeuLeu: 10.088 ± 0.114	1.623LeuMet: 1.623 ± 0.035	1.449LeuAsn: 1.449 ± 0.034	5.647LeuPro: 5.647 ± 0.067	2.187LeuGln: 2.187 ± 0.037	7.684LeuArg: 7.684 ± 0.083	5.396LeuSer: 5.396 ± 0.069	6.711LeuThr: 6.711 ± 0.076	10.732LeuVal: 10.732 ± 0.109	1.321LeuTrp: 1.321 ± 0.034	1.506LeuTyr: 1.506 ± 0.03	0.0LeuXaa: 0.0 ± 0.0
Met	2.245MetAla: 2.245 ± 0.042	0.109MetCys: 0.109 ± 0.008	0.869MetAsp: 0.869 ± 0.023	0.684MetGlu: 0.684 ± 0.025	0.454MetPhe: 0.454 ± 0.017	1.426MetGly: 1.426 ± 0.033	0.349MetHis: 0.349 ± 0.016	0.592MetIle: 0.592 ± 0.021	0.442MetLys: 0.442 ± 0.018	1.707MetLeu: 1.707 ± 0.041	0.292MetMet: 0.292 ± 0.015	0.381MetAsn: 0.381 ± 0.017	1.161MetPro: 1.161 ± 0.032	0.476MetGln: 0.476 ± 0.018	1.422MetArg: 1.422 ± 0.027	1.503MetSer: 1.503 ± 0.031	1.712MetThr: 1.712 ± 0.038	1.479MetVal: 1.479 ± 0.036	0.217MetTrp: 0.217 ± 0.013	0.263MetTyr: 0.263 ± 0.014	0.0MetXaa: 0.0 ± 0.0
Asn	2.247AsnAla: 2.247 ± 0.05	0.122AsnCys: 0.122 ± 0.009	1.007AsnAsp: 1.007 ± 0.03	0.811AsnGlu: 0.811 ± 0.026	0.458AsnPhe: 0.458 ± 0.018	1.772AsnGly: 1.772 ± 0.045	0.392AsnHis: 0.392 ± 0.014	0.616AsnIle: 0.616 ± 0.022	0.352AsnLys: 0.352 ± 0.017	1.709AsnLeu: 1.709 ± 0.04	0.254AsnMet: 0.254 ± 0.014	0.414AsnAsn: 0.414 ± 0.022	1.389AsnPro: 1.389 ± 0.031	0.476AsnGln: 0.476 ± 0.021	1.159AsnArg: 1.159 ± 0.027	0.785AsnSer: 0.785 ± 0.029	0.987AsnThr: 0.987 ± 0.031	1.572AsnVal: 1.572 ± 0.035	0.269AsnTrp: 0.269 ± 0.015	0.386AsnTyr: 0.386 ± 0.017	0.0AsnXaa: 0.0 ± 0.0
Pro	7.279ProAla: 7.279 ± 0.089	0.257ProCys: 0.257 ± 0.013	4.461ProAsp: 4.461 ± 0.061	3.72ProGlu: 3.72 ± 0.058	1.572ProPhe: 1.572 ± 0.036	5.837ProGly: 5.837 ± 0.073	1.207ProHis: 1.207 ± 0.027	1.68ProIle: 1.68 ± 0.039	1.104ProLys: 1.104 ± 0.032	4.74ProLeu: 4.74 ± 0.057	1.016ProMet: 1.016 ± 0.027	0.985ProAsn: 0.985 ± 0.027	2.842ProPro: 2.842 ± 0.059	1.454ProGln: 1.454 ± 0.033	3.661ProArg: 3.661 ± 0.05	3.593ProSer: 3.593 ± 0.058	4.192ProThr: 4.192 ± 0.064	5.372ProVal: 5.372 ± 0.067	0.934ProTrp: 0.934 ± 0.027	1.017ProTyr: 1.017 ± 0.026	0.001ProXaa: 0.001 ± 0.001
Gln	3.428GlnAla: 3.428 ± 0.054	0.177GlnCys: 0.177 ± 0.012	1.201GlnAsp: 1.201 ± 0.037	1.114GlnGlu: 1.114 ± 0.028	0.7GlnPhe: 0.7 ± 0.023	2.15GlnGly: 2.15 ± 0.04	0.605GlnHis: 0.605 ± 0.02	1.021GlnIle: 1.021 ± 0.024	0.581GlnLys: 0.581 ± 0.022	2.774GlnLeu: 2.774 ± 0.045	0.516GlnMet: 0.516 ± 0.018	0.454GlnAsn: 0.454 ± 0.018	1.601GlnPro: 1.601 ± 0.038	1.108GlnGln: 1.108 ± 0.037	2.324GlnArg: 2.324 ± 0.045	1.378GlnSer: 1.378 ± 0.033	1.538GlnThr: 1.538 ± 0.039	2.679GlnVal: 2.679 ± 0.045	0.509GlnTrp: 0.509 ± 0.019	0.481GlnTyr: 0.481 ± 0.021	0.0GlnXaa: 0.0 ± 0.0
Arg	9.188ArgAla: 9.188 ± 0.092	0.46ArgCys: 0.46 ± 0.022	4.679ArgAsp: 4.679 ± 0.059	4.31ArgGlu: 4.31 ± 0.069	2.203ArgPhe: 2.203 ± 0.044	5.746ArgGly: 5.746 ± 0.072	1.932ArgHis: 1.932 ± 0.035	2.941ArgIle: 2.941 ± 0.052	1.233ArgLys: 1.233 ± 0.032	8.212ArgLeu: 8.212 ± 0.089	1.667ArgMet: 1.667 ± 0.032	1.175ArgAsn: 1.175 ± 0.031	4.459ArgPro: 4.459 ± 0.06	1.99ArgGln: 1.99 ± 0.039	7.15ArgArg: 7.15 ± 0.094	4.458ArgSer: 4.458 ± 0.063	4.761ArgThr: 4.761 ± 0.059	6.449ArgVal: 6.449 ± 0.068	1.426ArgTrp: 1.426 ± 0.036	1.496ArgTyr: 1.496 ± 0.033	0.002ArgXaa: 0.002 ± 0.001
Ser	7.128SerAla: 7.128 ± 0.073	0.354SerCys: 0.354 ± 0.018	3.382SerAsp: 3.382 ± 0.054	2.467SerGlu: 2.467 ± 0.043	1.618SerPhe: 1.618 ± 0.037	6.139SerGly: 6.139 ± 0.082	1.124SerHis: 1.124 ± 0.029	1.86SerIle: 1.86 ± 0.038	1.055SerLys: 1.055 ± 0.033	5.358SerLeu: 5.358 ± 0.066	1.284SerMet: 1.284 ± 0.029	0.98SerAsn: 0.98 ± 0.029	3.28SerPro: 3.28 ± 0.049	1.408SerGln: 1.408 ± 0.033	4.15SerArg: 4.15 ± 0.053	3.559SerSer: 3.559 ± 0.063	3.847SerThr: 3.847 ± 0.062	4.869SerVal: 4.869 ± 0.061	0.965SerTrp: 0.965 ± 0.027	1.076SerTyr: 1.076 ± 0.03	0.001SerXaa: 0.001 ± 0.001
Thr	8.087ThrAla: 8.087 ± 0.105	0.447ThrCys: 0.447 ± 0.018	3.95ThrAsp: 3.95 ± 0.06	2.962ThrGlu: 2.962 ± 0.043	2.01ThrPhe: 2.01 ± 0.039	6.319ThrGly: 6.319 ± 0.084	1.296ThrHis: 1.296 ± 0.031	2.379ThrIle: 2.379 ± 0.041	1.349ThrLys: 1.349 ± 0.034	5.888ThrLeu: 5.888 ± 0.064	1.145ThrMet: 1.145 ± 0.032	1.232ThrAsn: 1.232 ± 0.033	4.302ThrPro: 4.302 ± 0.055	1.522ThrGln: 1.522 ± 0.036	4.212ThrArg: 4.212 ± 0.054	4.08ThrSer: 4.08 ± 0.062	4.897ThrThr: 4.897 ± 0.077	6.324ThrVal: 6.324 ± 0.085	1.141ThrTrp: 1.141 ± 0.028	1.42ThrTyr: 1.42 ± 0.038	0.001ThrXaa: 0.001 ± 0.001
Val	12.961ValAla: 12.961 ± 0.12	0.694ValCys: 0.694 ± 0.023	6.566ValAsp: 6.566 ± 0.068	5.34ValGlu: 5.34 ± 0.066	2.447ValPhe: 2.447 ± 0.045	8.371ValGly: 8.371 ± 0.081	2.068ValHis: 2.068 ± 0.041	3.403ValIle: 3.403 ± 0.054	1.702ValLys: 1.702 ± 0.038	9.995ValLeu: 9.995 ± 0.116	1.587ValMet: 1.587 ± 0.039	1.683ValAsn: 1.683 ± 0.038	5.325ValPro: 5.325 ± 0.067	2.093ValGln: 2.093 ± 0.041	6.89ValArg: 6.89 ± 0.08	5.195ValSer: 5.195 ± 0.072	6.767ValThr: 6.767 ± 0.083	11.37ValVal: 11.37 ± 0.13	1.243ValTrp: 1.243 ± 0.03	1.412ValTyr: 1.412 ± 0.033	0.0ValXaa: 0.0 ± 0.0
Trp	1.73TrpAla: 1.73 ± 0.041	0.161TrpCys: 0.161 ± 0.011	0.897TrpAsp: 0.897 ± 0.026	0.68TrpGlu: 0.68 ± 0.024	0.59TrpPhe: 0.59 ± 0.021	1.18TrpGly: 1.18 ± 0.031	0.384TrpHis: 0.384 ± 0.019	0.5TrpIle: 0.5 ± 0.017	0.297TrpLys: 0.297 ± 0.013	1.811TrpLeu: 1.811 ± 0.04	0.312TrpMet: 0.312 ± 0.014	0.363TrpAsn: 0.363 ± 0.016	0.819TrpPro: 0.819 ± 0.025	0.524TrpGln: 0.524 ± 0.018	1.305TrpArg: 1.305 ± 0.034	1.111TrpSer: 1.111 ± 0.031	1.079TrpThr: 1.079 ± 0.031	1.405TrpVal: 1.405 ± 0.03	0.386TrpTrp: 0.386 ± 0.017	0.32TrpTyr: 0.32 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.86TyrAla: 2.86 ± 0.042	0.128TyrCys: 0.128 ± 0.01	1.359TyrAsp: 1.359 ± 0.03	0.997TyrGlu: 0.997 ± 0.031	0.592TyrPhe: 0.592 ± 0.02	1.78TyrGly: 1.78 ± 0.034	0.326TyrHis: 0.326 ± 0.015	0.437TyrIle: 0.437 ± 0.019	0.321TyrLys: 0.321 ± 0.014	1.957TyrLeu: 1.957 ± 0.035	0.203TyrMet: 0.203 ± 0.014	0.377TyrAsn: 0.377 ± 0.02	0.946TyrPro: 0.946 ± 0.031	0.493TyrGln: 0.493 ± 0.024	1.498TyrArg: 1.498 ± 0.035	0.87TyrSer: 0.87 ± 0.024	0.965TyrThr: 0.965 ± 0.035	1.802TyrVal: 1.802 ± 0.035	0.317TyrTrp: 0.317 ± 0.017	0.432TyrTyr: 0.432 ± 0.018	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.001XaaHis: 0.001 ± 0.001	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.001XaaSer: 0.001 ± 0.001	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 4304 proteins (1430963 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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