Cyprinid herpesvirus 3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Alloherpesviridae; Cyprinivirus

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 169 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A4FTQ3|A4FTQ3_9VIRU Protein ORF156 OS=Cyprinid herpesvirus 3 OX=180230 GN=ORF156 PE=4 SV=1
MM1 pKa = 7.11VFSSVCVVFLAGTGTGTVLLGFAGTGTDD29 pKa = 3.11TGTVLAGAGAAFLTSITLVLGAGGSSSEE57 pKa = 4.13SSEE60 pKa = 4.14SEE62 pKa = 3.83EE63 pKa = 5.02AEE65 pKa = 4.09EE66 pKa = 4.19EE67 pKa = 4.11DD68 pKa = 4.25AEE70 pKa = 4.2EE71 pKa = 5.59DD72 pKa = 3.72EE73 pKa = 5.51EE74 pKa = 4.34EE75 pKa = 4.95DD76 pKa = 3.56SGCFLVALALPLEE89 pKa = 4.76GARR92 pKa = 11.84AVLPLILRR100 pKa = 11.84LMGGAGAFSCSDD112 pKa = 3.42SGSEE116 pKa = 4.74DD117 pKa = 3.32GGSSLLYY124 pKa = 10.24LSPPLSEE131 pKa = 4.63SEE133 pKa = 4.45SEE135 pKa = 4.4AEE137 pKa = 4.11DD138 pKa = 3.7SEE140 pKa = 4.82EE141 pKa = 3.84EE142 pKa = 4.13AEE144 pKa = 4.19EE145 pKa = 4.4AEE147 pKa = 4.29EE148 pKa = 4.59AEE150 pKa = 4.32EE151 pKa = 4.59AEE153 pKa = 4.45EE154 pKa = 4.71AEE156 pKa = 4.38EE157 pKa = 4.55ASDD160 pKa = 4.31SEE162 pKa = 4.97SEE164 pKa = 4.45AEE166 pKa = 4.23DD167 pKa = 3.97SEE169 pKa = 4.6TEE171 pKa = 4.05EE172 pKa = 6.02AEE174 pKa = 4.33DD175 pKa = 3.95TSTTSLDD182 pKa = 3.66LGLAGCFLGLVGATVVVVSSSSSEE206 pKa = 3.78EE207 pKa = 4.07SKK209 pKa = 11.18ALSAAGAGLLAGLPLPLPWPWPWLLAGCLWLISCACGIFTICAHH253 pKa = 5.61SQHH256 pKa = 5.9NTHH259 pKa = 6.06IVYY262 pKa = 10.51SIEE265 pKa = 3.75YY266 pKa = 8.98SNRR269 pKa = 11.84VV270 pKa = 3.11

Molecular weight:
27.54 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q52UN8|Q52UN8_9VIRU Major capsid protein OS=Cyprinid herpesvirus 3 OX=180230 GN=ORF92 PE=4 SV=1
MM1 pKa = 7.81SSRR4 pKa = 11.84CPYY7 pKa = 9.95SVSVLLEE14 pKa = 4.19SVKK17 pKa = 10.8SSALRR22 pKa = 11.84PTLIVLLDD30 pKa = 4.16SLWTWWTWWKK40 pKa = 10.61VEE42 pKa = 4.05HH43 pKa = 6.7GGHH46 pKa = 6.93GGRR49 pKa = 11.84TLPPYY54 pKa = 10.49FSLEE58 pKa = 4.03LNPNFSNCPNPNPNPNPNPNPNLTLALALTLTLFRR93 pKa = 11.84RR94 pKa = 11.84PRR96 pKa = 11.84RR97 pKa = 11.84RR98 pKa = 11.84EE99 pKa = 3.66TAPSRR104 pKa = 11.84VSAPHH109 pKa = 7.15DD110 pKa = 3.28GHH112 pKa = 8.13LLGGRR117 pKa = 11.84SLNGFKK123 pKa = 10.13TGFCSHH129 pKa = 6.87GGHH132 pKa = 6.0FQPYY136 pKa = 9.75LEE138 pKa = 5.05HH139 pKa = 6.51QNSSKK144 pKa = 10.52PLQKK148 pKa = 10.27QPLLLKK154 pKa = 9.23PTVSSPNWTQPYY166 pKa = 10.17HH167 pKa = 5.82FF168 pKa = 5.62

Molecular weight:
18.91 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

169

0

169

83578

52

3966

494.5

54.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.917 ± 0.157

2.144 ± 0.107

5.846 ± 0.115

6.034 ± 0.194

3.337 ± 0.097

6.14 ± 0.185

2.529 ± 0.101

3.252 ± 0.1

4.281 ± 0.206

8.822 ± 0.152

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.626 ± 0.096

3.299 ± 0.118

6.369 ± 0.173

3.904 ± 0.164

6.577 ± 0.178

7.596 ± 0.214

6.698 ± 0.265

7.477 ± 0.142

1.279 ± 0.066

2.872 ± 0.09

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski