Cyprinid herpesvirus 3
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 169 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A4FTQ3|A4FTQ3_9VIRU Protein ORF156 OS=Cyprinid herpesvirus 3 OX=180230 GN=ORF156 PE=4 SV=1
MM1 pKa = 7.11 VFSSVCVVFLAGTGTGTVLLGFAGTGTDD29 pKa = 3.11 TGTVLAGAGAAFLTSITLVLGAGGSSSEE57 pKa = 4.13 SSEE60 pKa = 4.14 SEE62 pKa = 3.83 EE63 pKa = 5.02 AEE65 pKa = 4.09 EE66 pKa = 4.19 EE67 pKa = 4.11 DD68 pKa = 4.25 AEE70 pKa = 4.2 EE71 pKa = 5.59 DD72 pKa = 3.72 EE73 pKa = 5.51 EE74 pKa = 4.34 EE75 pKa = 4.95 DD76 pKa = 3.56 SGCFLVALALPLEE89 pKa = 4.76 GARR92 pKa = 11.84 AVLPLILRR100 pKa = 11.84 LMGGAGAFSCSDD112 pKa = 3.42 SGSEE116 pKa = 4.74 DD117 pKa = 3.32 GGSSLLYY124 pKa = 10.24 LSPPLSEE131 pKa = 4.63 SEE133 pKa = 4.45 SEE135 pKa = 4.4 AEE137 pKa = 4.11 DD138 pKa = 3.7 SEE140 pKa = 4.82 EE141 pKa = 3.84 EE142 pKa = 4.13 AEE144 pKa = 4.19 EE145 pKa = 4.4 AEE147 pKa = 4.29 EE148 pKa = 4.59 AEE150 pKa = 4.32 EE151 pKa = 4.59 AEE153 pKa = 4.45 EE154 pKa = 4.71 AEE156 pKa = 4.38 EE157 pKa = 4.55 ASDD160 pKa = 4.31 SEE162 pKa = 4.97 SEE164 pKa = 4.45 AEE166 pKa = 4.23 DD167 pKa = 3.97 SEE169 pKa = 4.6 TEE171 pKa = 4.05 EE172 pKa = 6.02 AEE174 pKa = 4.33 DD175 pKa = 3.95 TSTTSLDD182 pKa = 3.66 LGLAGCFLGLVGATVVVVSSSSSEE206 pKa = 3.78 EE207 pKa = 4.07 SKK209 pKa = 11.18 ALSAAGAGLLAGLPLPLPWPWPWLLAGCLWLISCACGIFTICAHH253 pKa = 5.61 SQHH256 pKa = 5.9 NTHH259 pKa = 6.06 IVYY262 pKa = 10.51 SIEE265 pKa = 3.75 YY266 pKa = 8.98 SNRR269 pKa = 11.84 VV270 pKa = 3.11
Molecular weight: 27.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.0
IPC2_protein 3.528
IPC_protein 3.465
Toseland 3.3
ProMoST 3.579
Dawson 3.414
Bjellqvist 3.567
Wikipedia 3.274
Rodwell 3.3
Grimsley 3.21
Solomon 3.389
Lehninger 3.338
Nozaki 3.528
DTASelect 3.605
Thurlkill 3.325
EMBOSS 3.287
Sillero 3.567
Patrickios 0.121
IPC_peptide 3.401
IPC2_peptide 3.554
IPC2.peptide.svr19 3.585
Protein with the highest isoelectric point:
>tr|Q52UN8|Q52UN8_9VIRU Major capsid protein OS=Cyprinid herpesvirus 3 OX=180230 GN=ORF92 PE=4 SV=1
MM1 pKa = 7.81 SSRR4 pKa = 11.84 CPYY7 pKa = 9.95 SVSVLLEE14 pKa = 4.19 SVKK17 pKa = 10.8 SSALRR22 pKa = 11.84 PTLIVLLDD30 pKa = 4.16 SLWTWWTWWKK40 pKa = 10.61 VEE42 pKa = 4.05 HH43 pKa = 6.7 GGHH46 pKa = 6.93 GGRR49 pKa = 11.84 TLPPYY54 pKa = 10.49 FSLEE58 pKa = 4.03 LNPNFSNCPNPNPNPNPNPNPNLTLALALTLTLFRR93 pKa = 11.84 RR94 pKa = 11.84 PRR96 pKa = 11.84 RR97 pKa = 11.84 RR98 pKa = 11.84 EE99 pKa = 3.66 TAPSRR104 pKa = 11.84 VSAPHH109 pKa = 7.15 DD110 pKa = 3.28 GHH112 pKa = 8.13 LLGGRR117 pKa = 11.84 SLNGFKK123 pKa = 10.13 TGFCSHH129 pKa = 6.87 GGHH132 pKa = 6.0 FQPYY136 pKa = 9.75 LEE138 pKa = 5.05 HH139 pKa = 6.51 QNSSKK144 pKa = 10.52 PLQKK148 pKa = 10.27 QPLLLKK154 pKa = 9.23 PTVSSPNWTQPYY166 pKa = 10.17 HH167 pKa = 5.82 FF168 pKa = 5.62
Molecular weight: 18.91 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.276
IPC2_protein 9.443
IPC_protein 9.692
Toseland 10.101
ProMoST 9.823
Dawson 10.306
Bjellqvist 10.028
Wikipedia 10.496
Rodwell 10.628
Grimsley 10.379
Solomon 10.365
Lehninger 10.321
Nozaki 10.175
DTASelect 9.999
Thurlkill 10.175
EMBOSS 10.511
Sillero 10.248
Patrickios 10.321
IPC_peptide 10.365
IPC2_peptide 9.077
IPC2.peptide.svr19 8.316
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
169
0
169
83578
52
3966
494.5
54.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.917 ± 0.157
2.144 ± 0.107
5.846 ± 0.115
6.034 ± 0.194
3.337 ± 0.097
6.14 ± 0.185
2.529 ± 0.101
3.252 ± 0.1
4.281 ± 0.206
8.822 ± 0.152
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.626 ± 0.096
3.299 ± 0.118
6.369 ± 0.173
3.904 ± 0.164
6.577 ± 0.178
7.596 ± 0.214
6.698 ± 0.265
7.477 ± 0.142
1.279 ± 0.066
2.872 ± 0.09
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here