Porcine associated porprismacovirus 9
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A076VEP0|A0A076VEP0_9VIRU Cap protein OS=Porcine associated porprismacovirus 9 OX=2170125 PE=4 SV=1
MM1 pKa = 7.26 ICIPRR6 pKa = 11.84 KK7 pKa = 10.16 AFLLCWSFLLLMVFVLLGILLGFFAQFRR35 pKa = 11.84 KK36 pKa = 9.6 FRR38 pKa = 11.84 YY39 pKa = 9.49 AGAKK43 pKa = 7.69 VTFIPAATLPADD55 pKa = 4.06 PLQVSYY61 pKa = 10.83 EE62 pKa = 4.08 AGEE65 pKa = 4.0 PTIDD69 pKa = 5.06 PRR71 pKa = 11.84 DD72 pKa = 3.66 LLNPILHH79 pKa = 6.5 KK80 pKa = 10.66 GIHH83 pKa = 6.41 GDD85 pKa = 3.29 ALGNLLEE92 pKa = 4.73 EE93 pKa = 4.58 YY94 pKa = 10.34 KK95 pKa = 10.56 QVLASGSSLEE105 pKa = 4.16 LTKK108 pKa = 10.2 TAAANYY114 pKa = 5.74 MTLYY118 pKa = 8.82 YY119 pKa = 10.33 QCLTDD124 pKa = 4.1 PSFKK128 pKa = 10.54 KK129 pKa = 10.47 SGLRR133 pKa = 11.84 QGFVQNLYY141 pKa = 9.99 PLVYY145 pKa = 10.58 SLGTNRR151 pKa = 11.84 QIMPSRR157 pKa = 11.84 IFTAGASGLTVGDD170 pKa = 3.99 PSLMEE175 pKa = 3.84 TGKK178 pKa = 10.16 IFSEE182 pKa = 4.09 TDD184 pKa = 2.99 GQFEE188 pKa = 4.8 YY189 pKa = 11.17 NDD191 pKa = 3.78 EE192 pKa = 4.21 TWGVSVRR199 pKa = 11.84 RR200 pKa = 11.84 SLGQAQQNLKK210 pKa = 10.96 DD211 pKa = 4.51 NAIEE215 pKa = 3.89 WKK217 pKa = 9.37 TYY219 pKa = 11.08 DD220 pKa = 3.76 EE221 pKa = 4.68 FTTKK225 pKa = 9.01 PTRR228 pKa = 11.84 LGWLDD233 pKa = 3.31 TLQIIRR239 pKa = 11.84 DD240 pKa = 4.14 NQSSMPDD247 pKa = 3.32 SQVTGVNIEE256 pKa = 3.82 LSIRR260 pKa = 11.84 NPQDD264 pKa = 3.21 PLASRR269 pKa = 11.84 VTPCNFTTLPKK280 pKa = 10.03 IPMYY284 pKa = 10.3 MCFLPPAYY292 pKa = 8.44 KK293 pKa = 9.61 TEE295 pKa = 3.81 MYY297 pKa = 10.14 FRR299 pKa = 11.84 IVIQHH304 pKa = 5.02 YY305 pKa = 10.47 FEE307 pKa = 4.84 FKK309 pKa = 10.34 EE310 pKa = 4.12 FRR312 pKa = 11.84 STFNQFGIGQVSTATDD328 pKa = 3.55 VPYY331 pKa = 11.05 DD332 pKa = 3.4 WLNAIKK338 pKa = 10.12 KK339 pKa = 9.68 VSTASVEE346 pKa = 4.4 SEE348 pKa = 4.08 KK349 pKa = 11.27 SLDD352 pKa = 3.46 IAGGDD357 pKa = 4.0 AEE359 pKa = 4.49 LTTDD363 pKa = 3.97 GVLL366 pKa = 3.65
Molecular weight: 40.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.299
IPC2_protein 5.486
IPC_protein 5.41
Toseland 5.423
ProMoST 5.601
Dawson 5.461
Bjellqvist 5.563
Wikipedia 5.359
Rodwell 5.385
Grimsley 5.385
Solomon 5.461
Lehninger 5.423
Nozaki 5.614
DTASelect 5.779
Thurlkill 5.499
EMBOSS 5.461
Sillero 5.69
Patrickios 3.961
IPC_peptide 5.474
IPC2_peptide 5.677
IPC2.peptide.svr19 5.76
Protein with the highest isoelectric point:
>tr|A0A076VEP0|A0A076VEP0_9VIRU Cap protein OS=Porcine associated porprismacovirus 9 OX=2170125 PE=4 SV=1
MM1 pKa = 8.06 RR2 pKa = 11.84 INAKK6 pKa = 8.49 CTLVRR11 pKa = 11.84 TIHH14 pKa = 6.67 HH15 pKa = 7.33 KK16 pKa = 9.8 DD17 pKa = 2.81 TLRR20 pKa = 11.84 YY21 pKa = 10.37 KK22 pKa = 10.12 MVTNYY27 pKa = 9.76 MITWSRR33 pKa = 11.84 NDD35 pKa = 3.05 TYY37 pKa = 11.53 KK38 pKa = 10.67 QFNQLMRR45 pKa = 11.84 LIRR48 pKa = 11.84 KK49 pKa = 9.19 NDD51 pKa = 2.77 IHH53 pKa = 7.31 RR54 pKa = 11.84 WIVGIEE60 pKa = 4.02 TGTNGYY66 pKa = 9.6 DD67 pKa = 2.99 HH68 pKa = 6.68 FQIRR72 pKa = 11.84 MATRR76 pKa = 11.84 WTFDD80 pKa = 2.72 QMTEE84 pKa = 3.76 IFHH87 pKa = 5.83 GQAHH91 pKa = 6.37 IEE93 pKa = 4.23 EE94 pKa = 5.32 CSDD97 pKa = 2.52 TWEE100 pKa = 4.21 YY101 pKa = 10.56 EE102 pKa = 4.05 RR103 pKa = 11.84 KK104 pKa = 10.09 SGIFFSSEE112 pKa = 3.21 DD113 pKa = 3.37 TAEE116 pKa = 3.67 IRR118 pKa = 11.84 RR119 pKa = 11.84 VRR121 pKa = 11.84 FGHH124 pKa = 6.45 PNQAQEE130 pKa = 4.75 HH131 pKa = 6.24 IIEE134 pKa = 4.37 TARR137 pKa = 11.84 GQNDD141 pKa = 3.47 RR142 pKa = 11.84 QVDD145 pKa = 3.15 VWYY148 pKa = 10.04 DD149 pKa = 3.3 PKK151 pKa = 11.36 GNHH154 pKa = 5.59 GKK156 pKa = 7.72 TWLTVHH162 pKa = 5.87 LWEE165 pKa = 5.31 RR166 pKa = 11.84 GQALVVPRR174 pKa = 11.84 SSATPEE180 pKa = 3.89 KK181 pKa = 10.46 LSAFICSAYY190 pKa = 9.79 KK191 pKa = 10.62 GEE193 pKa = 4.17 EE194 pKa = 4.13 FIIIDD199 pKa = 3.62 IPRR202 pKa = 11.84 AGKK205 pKa = 9.94 IPPSLYY211 pKa = 10.52 EE212 pKa = 3.93 VIEE215 pKa = 4.1 EE216 pKa = 4.34 VKK218 pKa = 10.79 DD219 pKa = 3.6 GLVFDD224 pKa = 4.47 HH225 pKa = 7.49 RR226 pKa = 11.84 YY227 pKa = 7.42 TGKK230 pKa = 8.17 TRR232 pKa = 11.84 NIRR235 pKa = 11.84 GAKK238 pKa = 9.87 VIIFTNTKK246 pKa = 10.09 LDD248 pKa = 3.94 EE249 pKa = 4.79 KK250 pKa = 10.7 KK251 pKa = 10.61 LSYY254 pKa = 10.66 DD255 pKa = 2.72 RR256 pKa = 11.84 WRR258 pKa = 11.84 LHH260 pKa = 5.49 GMEE263 pKa = 4.8 KK264 pKa = 10.41 GGEE267 pKa = 4.21 GEE269 pKa = 4.12 PLSS272 pKa = 4.04
Molecular weight: 31.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.318
IPC2_protein 8.258
IPC_protein 8.244
Toseland 8.741
ProMoST 8.682
Dawson 9.077
Bjellqvist 8.99
Wikipedia 9.209
Rodwell 9.209
Grimsley 9.019
Solomon 9.209
Lehninger 9.18
Nozaki 9.092
DTASelect 8.887
Thurlkill 8.99
EMBOSS 9.238
Sillero 9.165
Patrickios 4.762
IPC_peptide 9.209
IPC2_peptide 7.732
IPC2.peptide.svr19 7.63
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
638
272
366
319.0
36.48
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.643 ± 0.781
1.254 ± 0.096
5.329 ± 0.118
6.113 ± 1.02
5.172 ± 0.716
6.897 ± 0.056
2.351 ± 1.307
7.053 ± 1.123
5.643 ± 0.618
8.621 ± 2.203
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.508 ± 0.042
3.918 ± 0.08
4.545 ± 1.017
4.389 ± 0.452
5.956 ± 1.585
5.799 ± 0.88
7.837 ± 0.074
5.016 ± 0.15
2.038 ± 0.573
3.918 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here