Sporomusa silvacetica DSM 10669

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Sporomusa; Sporomusa silvacetica

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5636 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A259UF21|A0A259UF21_9FIRM Beta-1 4-mannooligosaccharide phosphorylase OS=Sporomusa silvacetica DSM 10669 OX=1123289 GN=SPSIL_35880 PE=4 SV=1
MM1 pKa = 7.43PSEE4 pKa = 4.13VEE6 pKa = 4.13DD7 pKa = 4.78RR8 pKa = 11.84LDD10 pKa = 3.84LGCLVGSLTATITTKK25 pKa = 10.73CGIVYY30 pKa = 10.28QGTILDD36 pKa = 4.24WNCGGITNARR46 pKa = 11.84PDD48 pKa = 3.65SSSSDD53 pKa = 3.25SGDD56 pKa = 3.57DD57 pKa = 3.44QKK59 pKa = 11.56EE60 pKa = 4.31SSPSPRR66 pKa = 11.84MGGDD70 pKa = 2.87NCRR73 pKa = 11.84SFIRR77 pKa = 11.84MEE79 pKa = 4.54LGCQPGNICCPLFTNTLNGSLVIGDD104 pKa = 4.14DD105 pKa = 3.4GGIIEE110 pKa = 4.82FTLDD114 pKa = 3.27APATQSRR121 pKa = 11.84VTAIRR126 pKa = 11.84GTDD129 pKa = 3.15ADD131 pKa = 3.66IPLYY135 pKa = 10.79AVGSTVLINWDD146 pKa = 3.85DD147 pKa = 3.63VSSIGSIGTTEE158 pKa = 3.99EE159 pKa = 4.64CLTPSPSAATSPP171 pKa = 3.56

Molecular weight: 17.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A259UCU6|A0A259UCU6_9FIRM Uncharacterized protein OS=Sporomusa silvacetica DSM 10669 OX=1123289 GN=SPSIL_39750 PE=4 SV=1
MM1 pKa = 7.65HH2 pKa = 7.01MGEE5 pKa = 4.16GQGRR9 pKa = 11.84RR10 pKa = 11.84EE11 pKa = 3.87EE12 pKa = 4.13LGKK15 pKa = 9.64QRR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84TFRR22 pKa = 11.84CIGDD26 pKa = 3.45GMYY29 pKa = 10.41RR30 pKa = 11.84KK31 pKa = 9.93YY32 pKa = 10.77SSEE35 pKa = 4.17LEE37 pKa = 4.18RR38 pKa = 11.84PSPARR43 pKa = 11.84EE44 pKa = 3.72TVRR47 pKa = 11.84RR48 pKa = 11.84KK49 pKa = 10.16HH50 pKa = 6.12ISQTVKK56 pKa = 10.9LLFCRR61 pKa = 11.84KK62 pKa = 8.75GVRR65 pKa = 11.84GVHH68 pKa = 5.17ITDD71 pKa = 3.19NAMDD75 pKa = 3.97NKK77 pKa = 9.89TIARR81 pKa = 11.84EE82 pKa = 4.16GTLLHH87 pKa = 6.9SGFF90 pKa = 4.36

Molecular weight: 10.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5636

0

5636

1641199

29

2667

291.2

32.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.875 ± 0.037

1.352 ± 0.017

4.917 ± 0.024

6.189 ± 0.032

3.852 ± 0.023

7.383 ± 0.033

1.811 ± 0.015

7.628 ± 0.03

6.012 ± 0.033

9.681 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.732 ± 0.016

4.256 ± 0.025

3.906 ± 0.023

3.864 ± 0.021

4.388 ± 0.023

5.724 ± 0.03

5.689 ± 0.03

7.385 ± 0.028

0.999 ± 0.013

3.355 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski