Genomoviridae sp.
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A345N0J8|A0A345N0J8_9VIRU Rep catalytic domain protein OS=Genomoviridae sp. OX=2202565 PE=3 SV=1
MM1 pKa = 7.4 TFDD4 pKa = 3.03 FHH6 pKa = 7.8 CRR8 pKa = 11.84 YY9 pKa = 10.74 GLFTYY14 pKa = 7.66 SQCTGLDD21 pKa = 3.23 PWHH24 pKa = 6.51 VLDD27 pKa = 4.79 KK28 pKa = 10.79 FSEE31 pKa = 4.04 IGAEE35 pKa = 4.04 CIIARR40 pKa = 11.84 EE41 pKa = 4.04 AHH43 pKa = 6.55 ADD45 pKa = 3.58 GGTHH49 pKa = 6.19 LHH51 pKa = 6.59 VFADD55 pKa = 3.35 WGRR58 pKa = 11.84 RR59 pKa = 11.84 KK60 pKa = 10.07 RR61 pKa = 11.84 FRR63 pKa = 11.84 RR64 pKa = 11.84 HH65 pKa = 6.15 DD66 pKa = 3.67 FADD69 pKa = 3.18 VDD71 pKa = 4.21 SFHH74 pKa = 7.12 PNIVPSRR81 pKa = 11.84 GTPHH85 pKa = 7.33 LGWDD89 pKa = 3.69 YY90 pKa = 10.71 ATKK93 pKa = 10.79 DD94 pKa = 3.25 GDD96 pKa = 3.7 IVAGGLEE103 pKa = 4.28 RR104 pKa = 11.84 PGGSCDD110 pKa = 3.06 NLLRR114 pKa = 11.84 KK115 pKa = 9.79 DD116 pKa = 4.48 HH117 pKa = 6.64 IWNQIMDD124 pKa = 3.94 AVTRR128 pKa = 11.84 EE129 pKa = 3.85 QFFEE133 pKa = 4.3 LVRR136 pKa = 11.84 EE137 pKa = 4.3 LAPADD142 pKa = 3.69 LAKK145 pKa = 10.71 SFPSLSKK152 pKa = 10.52 YY153 pKa = 10.8 ADD155 pKa = 2.85 WRR157 pKa = 11.84 YY158 pKa = 10.63 AEE160 pKa = 4.47 PLATYY165 pKa = 8.2 EE166 pKa = 4.51 GPTFGDD172 pKa = 2.86 IRR174 pKa = 11.84 FDD176 pKa = 3.86 LSPYY180 pKa = 9.73 PGLEE184 pKa = 3.55 QWCNDD189 pKa = 2.31 IRR191 pKa = 11.84 AGSNGQSGTYY201 pKa = 9.83
Molecular weight: 22.84 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.341
IPC2_protein 5.296
IPC_protein 5.296
Toseland 5.601
ProMoST 5.601
Dawson 5.486
Bjellqvist 5.525
Wikipedia 5.461
Rodwell 5.436
Grimsley 5.715
Solomon 5.486
Lehninger 5.461
Nozaki 5.703
DTASelect 5.906
Thurlkill 5.804
EMBOSS 5.779
Sillero 5.792
Patrickios 3.834
IPC_peptide 5.499
IPC2_peptide 5.804
IPC2.peptide.svr19 5.769
Protein with the highest isoelectric point:
>tr|A0A345N0J7|A0A345N0J7_9VIRU Uncharacterized protein OS=Genomoviridae sp. OX=2202565 PE=4 SV=1
MM1 pKa = 6.37 ATKK4 pKa = 10.45 RR5 pKa = 11.84 IIRR8 pKa = 11.84 PRR10 pKa = 11.84 RR11 pKa = 11.84 GNRR14 pKa = 11.84 RR15 pKa = 11.84 FVKK18 pKa = 10.43 RR19 pKa = 11.84 KK20 pKa = 8.89 ARR22 pKa = 11.84 PAARR26 pKa = 11.84 SRR28 pKa = 11.84 RR29 pKa = 11.84 RR30 pKa = 11.84 PMKK33 pKa = 9.92 RR34 pKa = 11.84 GSMSRR39 pKa = 11.84 KK40 pKa = 9.05 RR41 pKa = 11.84 ILNITSRR48 pKa = 11.84 KK49 pKa = 8.82 KK50 pKa = 9.49 QDD52 pKa = 4.09 NMRR55 pKa = 11.84 SWNGTPFLAGSAGPIVMQGDD75 pKa = 4.0 DD76 pKa = 3.51 SYY78 pKa = 11.72 MFGFVCTARR87 pKa = 11.84 DD88 pKa = 3.52 KK89 pKa = 11.58 DD90 pKa = 4.33 LGTGPAPIEE99 pKa = 4.01 MDD101 pKa = 3.61 PSRR104 pKa = 11.84 TSTHH108 pKa = 6.39 CYY110 pKa = 8.37 MRR112 pKa = 11.84 GFKK115 pKa = 10.29 EE116 pKa = 4.69 RR117 pKa = 11.84 IDD119 pKa = 4.22 LFTGSAHH126 pKa = 5.37 PWIWRR131 pKa = 11.84 RR132 pKa = 11.84 ILFTYY137 pKa = 10.06 KK138 pKa = 10.69 GLDD141 pKa = 3.63 LLRR144 pKa = 11.84 VTDD147 pKa = 4.87 DD148 pKa = 4.15 EE149 pKa = 5.12 SPGDD153 pKa = 4.04 DD154 pKa = 3.68 GVGQLWVEE162 pKa = 4.11 SSDD165 pKa = 3.81 GYY167 pKa = 10.74 GRR169 pKa = 11.84 YY170 pKa = 8.74 YY171 pKa = 11.4 ANIGDD176 pKa = 4.01 PTDD179 pKa = 4.04 LKK181 pKa = 11.25 SSVIGQHH188 pKa = 5.84 IRR190 pKa = 11.84 DD191 pKa = 4.7 LIFKK195 pKa = 8.17 GTAEE199 pKa = 4.19 KK200 pKa = 10.52 DD201 pKa = 3.61 YY202 pKa = 10.85 TSSFSAKK209 pKa = 9.1 TDD211 pKa = 3.36 QEE213 pKa = 4.33 RR214 pKa = 11.84 VKK216 pKa = 10.47 ILYY219 pKa = 9.93 DD220 pKa = 3.12 KK221 pKa = 10.79 TVTIKK226 pKa = 10.55 SGNDD230 pKa = 2.48 RR231 pKa = 11.84 GVIKK235 pKa = 9.44 TYY237 pKa = 10.62 RR238 pKa = 11.84 RR239 pKa = 11.84 WHH241 pKa = 5.65 GFNRR245 pKa = 11.84 TLIYY249 pKa = 11.03 DD250 pKa = 3.75 EE251 pKa = 5.68 DD252 pKa = 4.0 EE253 pKa = 4.62 VGAQTANRR261 pKa = 11.84 LLSSQSPRR269 pKa = 11.84 SMGDD273 pKa = 3.0 VYY275 pKa = 11.43 VIDD278 pKa = 4.77 FFSPGLGGTSDD289 pKa = 3.97 DD290 pKa = 4.35 EE291 pKa = 4.97 LRR293 pKa = 11.84 LLPTATLYY301 pKa = 8.93 WHH303 pKa = 6.59 EE304 pKa = 4.22 RR305 pKa = 3.47
Molecular weight: 34.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.21
IPC2_protein 9.341
IPC_protein 9.531
Toseland 10.028
ProMoST 9.809
Dawson 10.248
Bjellqvist 9.94
Wikipedia 10.438
Rodwell 10.526
Grimsley 10.321
Solomon 10.277
Lehninger 10.233
Nozaki 10.028
DTASelect 9.926
Thurlkill 10.087
EMBOSS 10.438
Sillero 10.16
Patrickios 9.97
IPC_peptide 10.277
IPC2_peptide 8.609
IPC2.peptide.svr19 8.414
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
738
201
305
246.0
27.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.504 ± 0.728
1.355 ± 0.381
7.317 ± 1.395
4.065 ± 0.371
4.472 ± 0.655
8.537 ± 0.663
3.252 ± 0.744
5.556 ± 0.347
3.659 ± 1.155
9.485 ± 2.282
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.252 ± 1.018
2.71 ± 0.126
4.336 ± 0.556
3.117 ± 0.66
8.13 ± 1.788
9.485 ± 2.142
6.098 ± 0.515
3.794 ± 0.219
2.168 ± 0.271
2.71 ± 1.096
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here