Tomato leaf curl Yunnan betasatellite
Average proteome isoelectric point is 4.87
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2U8UW19|A0A2U8UW19_9VIRU Beta C1 OS=Tomato leaf curl Yunnan betasatellite OX=2203894 GN=C1 PE=4 SV=1
MM1 pKa = 7.23 TIKK4 pKa = 10.81 YY5 pKa = 9.56 NNKK8 pKa = 9.52 NGLEE12 pKa = 3.91 FTIDD16 pKa = 3.3 VRR18 pKa = 11.84 LEE20 pKa = 3.76 EE21 pKa = 4.22 HH22 pKa = 6.24 TSFIVQVKK30 pKa = 8.94 IWSTRR35 pKa = 11.84 APALAKK41 pKa = 9.99 KK42 pKa = 10.38 KK43 pKa = 10.61 FMIPYY48 pKa = 7.9 RR49 pKa = 11.84 HH50 pKa = 6.24 NGIVAPVDD58 pKa = 4.11 FNSLEE63 pKa = 4.12 EE64 pKa = 4.82 GIKK67 pKa = 9.95 HH68 pKa = 6.67 CIKK71 pKa = 10.46 IMYY74 pKa = 9.16 KK75 pKa = 10.48 NSTIWEE81 pKa = 4.63 FKK83 pKa = 9.91 QEE85 pKa = 4.01 DD86 pKa = 4.48 MIDD89 pKa = 4.05 VIDD92 pKa = 3.88 MLMMEE97 pKa = 5.04 EE98 pKa = 4.73 APVEE102 pKa = 4.17 DD103 pKa = 4.16 MDD105 pKa = 5.44 VGDD108 pKa = 4.86 EE109 pKa = 4.09 YY110 pKa = 11.76 DD111 pKa = 3.82 VIIGSSVV118 pKa = 2.54
Molecular weight: 13.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.867
IPC2_protein 4.889
IPC_protein 4.774
Toseland 4.66
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.596
Rodwell 4.635
Grimsley 4.571
Solomon 4.724
Lehninger 4.685
Nozaki 4.851
DTASelect 4.991
Thurlkill 4.66
EMBOSS 4.622
Sillero 4.914
Patrickios 4.075
IPC_peptide 4.736
IPC2_peptide 4.902
IPC2.peptide.svr19 4.907
Protein with the highest isoelectric point:
>tr|A0A2U8UW19|A0A2U8UW19_9VIRU Beta C1 OS=Tomato leaf curl Yunnan betasatellite OX=2203894 GN=C1 PE=4 SV=1
MM1 pKa = 7.23 TIKK4 pKa = 10.81 YY5 pKa = 9.56 NNKK8 pKa = 9.52 NGLEE12 pKa = 3.91 FTIDD16 pKa = 3.3 VRR18 pKa = 11.84 LEE20 pKa = 3.76 EE21 pKa = 4.22 HH22 pKa = 6.24 TSFIVQVKK30 pKa = 8.94 IWSTRR35 pKa = 11.84 APALAKK41 pKa = 9.99 KK42 pKa = 10.38 KK43 pKa = 10.61 FMIPYY48 pKa = 7.9 RR49 pKa = 11.84 HH50 pKa = 6.24 NGIVAPVDD58 pKa = 4.11 FNSLEE63 pKa = 4.12 EE64 pKa = 4.82 GIKK67 pKa = 9.95 HH68 pKa = 6.67 CIKK71 pKa = 10.46 IMYY74 pKa = 9.16 KK75 pKa = 10.48 NSTIWEE81 pKa = 4.63 FKK83 pKa = 9.91 QEE85 pKa = 4.01 DD86 pKa = 4.48 MIDD89 pKa = 4.05 VIDD92 pKa = 3.88 MLMMEE97 pKa = 5.04 EE98 pKa = 4.73 APVEE102 pKa = 4.17 DD103 pKa = 4.16 MDD105 pKa = 5.44 VGDD108 pKa = 4.86 EE109 pKa = 4.09 YY110 pKa = 11.76 DD111 pKa = 3.82 VIIGSSVV118 pKa = 2.54
Molecular weight: 13.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.867
IPC2_protein 4.889
IPC_protein 4.774
Toseland 4.66
ProMoST 4.889
Dawson 4.736
Bjellqvist 4.876
Wikipedia 4.596
Rodwell 4.635
Grimsley 4.571
Solomon 4.724
Lehninger 4.685
Nozaki 4.851
DTASelect 4.991
Thurlkill 4.66
EMBOSS 4.622
Sillero 4.914
Patrickios 4.075
IPC_peptide 4.736
IPC2_peptide 4.902
IPC2.peptide.svr19 4.907
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
118
118
118
118.0
13.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.237 ± 0.0
0.847 ± 0.0
7.627 ± 0.0
9.322 ± 0.0
4.237 ± 0.0
4.237 ± 0.0
2.542 ± 0.0
11.864 ± 0.0
8.475 ± 0.0
4.237 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
6.78 ± 0.0
5.085 ± 0.0
3.39 ± 0.0
1.695 ± 0.0
2.542 ± 0.0
5.085 ± 0.0
4.237 ± 0.0
8.475 ± 0.0
1.695 ± 0.0
3.39 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here