Actinotalea ferrariae CF5-4

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Actinotalea; Actinotalea ferrariae

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3558 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A021VSH3|A0A021VSH3_9CELL Permease (Fragment) OS=Actinotalea ferrariae CF5-4 OX=948458 GN=N866_15590 PE=4 SV=1
MM1 pKa = 6.84VAALALTACAPASSEE16 pKa = 5.13DD17 pKa = 3.67PTEE20 pKa = 4.49PPAEE24 pKa = 4.07TAGASDD30 pKa = 5.55DD31 pKa = 4.02EE32 pKa = 4.91TGDD35 pKa = 3.57EE36 pKa = 4.86APAQAEE42 pKa = 4.41GTLKK46 pKa = 10.57VGYY49 pKa = 9.77ISPITGNFAVAGQEE63 pKa = 4.74MVDD66 pKa = 3.15GWNLYY71 pKa = 9.1WEE73 pKa = 4.49INGNEE78 pKa = 4.12VNGVTVEE85 pKa = 4.41TIVEE89 pKa = 4.09DD90 pKa = 3.97DD91 pKa = 3.94AGNPEE96 pKa = 3.78ISLTKK101 pKa = 10.09ARR103 pKa = 11.84KK104 pKa = 8.97LVEE107 pKa = 4.1SDD109 pKa = 3.3QVDD112 pKa = 4.19VVVGPLLANTALAVAEE128 pKa = 4.31YY129 pKa = 8.79TSSVGVPNLHH139 pKa = 6.58PVAAADD145 pKa = 5.2DD146 pKa = 4.06LTQRR150 pKa = 11.84AANDD154 pKa = 3.25FTVRR158 pKa = 11.84SGSMSGSQANYY169 pKa = 9.66PGGVWAADD177 pKa = 3.57QGHH180 pKa = 5.51EE181 pKa = 4.31TAVTLCPDD189 pKa = 3.51YY190 pKa = 11.55AFGWEE195 pKa = 4.02SCAGFKK201 pKa = 10.36QGFEE205 pKa = 4.15QNGGEE210 pKa = 4.17VTAQFWYY217 pKa = 9.94PNGTSDD223 pKa = 3.77FSTYY227 pKa = 9.87VSQLQSAGADD237 pKa = 2.89IAYY240 pKa = 9.94VATAGGAPGPDD251 pKa = 3.61FMRR254 pKa = 11.84SFLGLGLKK262 pKa = 8.57DD263 pKa = 3.78TQPLLMNCCSMDD275 pKa = 3.51QATLRR280 pKa = 11.84AMGTEE285 pKa = 3.86VLGIHH290 pKa = 6.31SVSNWAEE297 pKa = 3.73GRR299 pKa = 11.84DD300 pKa = 3.79APEE303 pKa = 3.65VAEE306 pKa = 4.85FNAAYY311 pKa = 10.37AEE313 pKa = 4.36RR314 pKa = 11.84YY315 pKa = 9.06DD316 pKa = 4.46GKK318 pKa = 10.68IPSLNVAGGYY328 pKa = 7.45VTASQVAQVLAEE340 pKa = 4.19NGLVTGDD347 pKa = 3.91EE348 pKa = 4.15LVAAISATTFEE359 pKa = 4.93DD360 pKa = 3.82SILGKK365 pKa = 8.28TVFDD369 pKa = 4.51EE370 pKa = 4.51YY371 pKa = 11.26NNPVSPVYY379 pKa = 10.05IRR381 pKa = 11.84EE382 pKa = 4.05VQEE385 pKa = 4.37VDD387 pKa = 3.33GQLLNVPIEE396 pKa = 4.19TFDD399 pKa = 6.05AVDD402 pKa = 3.02QWLGMDD408 pKa = 4.4PEE410 pKa = 4.27EE411 pKa = 5.55AMARR415 pKa = 11.84PNYY418 pKa = 9.48SQQFQGG424 pKa = 3.76

Molecular weight: 44.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A021VQV5|A0A021VQV5_9CELL UPF0301 protein N866_19500 OS=Actinotalea ferrariae CF5-4 OX=948458 GN=N866_19500 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 8.7THH17 pKa = 5.15GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILAARR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.97GRR40 pKa = 11.84TEE42 pKa = 3.99LSAA45 pKa = 4.86

Molecular weight: 5.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3558

0

3558

1116291

35

1964

313.7

33.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.285 ± 0.062

0.591 ± 0.011

6.395 ± 0.035

5.634 ± 0.036

2.466 ± 0.027

9.427 ± 0.036

2.245 ± 0.022

2.692 ± 0.027

1.29 ± 0.027

10.503 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.614 ± 0.016

1.389 ± 0.018

6.076 ± 0.035

2.659 ± 0.019

8.269 ± 0.043

4.588 ± 0.022

6.27 ± 0.033

10.377 ± 0.045

1.497 ± 0.017

1.73 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski