Fusarium oxysporum NRRL 32931
Average proteome isoelectric point is 6.44
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 20767 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W9HMB3|W9HMB3_FUSOX Uncharacterized protein OS=Fusarium oxysporum NRRL 32931 OX=660029 GN=FOYG_14246 PE=4 SV=1
MM1 pKa = 7.6 RR2 pKa = 11.84 FSVLFAFGLVTNVANASVCKK22 pKa = 9.81 PRR24 pKa = 11.84 PSDD27 pKa = 3.44 SSSASSVEE35 pKa = 4.17 SVTLSISATAPTTTVAAEE53 pKa = 4.18 TTTATSADD61 pKa = 3.78 LSSTFASTVGNEE73 pKa = 4.06 TTTEE77 pKa = 4.44 AIEE80 pKa = 4.24 TATTTDD86 pKa = 3.89 TITTAATTTAEE97 pKa = 4.27 TTTADD102 pKa = 3.44 ATTTSADD109 pKa = 3.44 TFVPIPTFDD118 pKa = 3.3 VLAIGAQVDD127 pKa = 4.16 GQKK130 pKa = 10.67 LRR132 pKa = 11.84 GHH134 pKa = 5.52 VTTDD138 pKa = 3.3 YY139 pKa = 11.72 GMGWNLDD146 pKa = 3.54 RR147 pKa = 11.84 TPPILAFSIDD157 pKa = 3.69 PDD159 pKa = 4.04 TNQVKK164 pKa = 9.49 EE165 pKa = 4.01 VNGNYY170 pKa = 10.39 LCLQYY175 pKa = 11.33 GDD177 pKa = 4.72 PNEE180 pKa = 5.15 DD181 pKa = 3.32 YY182 pKa = 11.3 PNFLKK187 pKa = 10.96 LCDD190 pKa = 4.45 PGSVTNIDD198 pKa = 3.32 IGLGMVTCEE207 pKa = 3.75 QTRR210 pKa = 11.84 DD211 pKa = 3.32 RR212 pKa = 11.84 RR213 pKa = 11.84 LEE215 pKa = 4.12 CSTPAAQCVEE225 pKa = 4.31 DD226 pKa = 4.9 DD227 pKa = 3.65 MTRR230 pKa = 11.84 MVTCSEE236 pKa = 4.19 LPGTFTGFYY245 pKa = 8.88 TYY247 pKa = 11.04 SGMSEE252 pKa = 4.75 GITLVMGPEE261 pKa = 4.5 SNGPTGTAYY270 pKa = 10.53 QSVDD274 pKa = 3.62 LGIEE278 pKa = 3.99 PASQQ282 pKa = 2.85
Molecular weight: 29.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.725
IPC2_protein 3.897
IPC_protein 3.884
Toseland 3.668
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.783
Rodwell 3.706
Grimsley 3.579
Solomon 3.859
Lehninger 3.808
Nozaki 3.973
DTASelect 4.19
Thurlkill 3.719
EMBOSS 3.795
Sillero 3.999
Patrickios 1.138
IPC_peptide 3.859
IPC2_peptide 3.986
IPC2.peptide.svr19 3.893
Protein with the highest isoelectric point:
>tr|W9IYV0|W9IYV0_FUSOX Uncharacterized protein OS=Fusarium oxysporum NRRL 32931 OX=660029 GN=FOYG_02598 PE=4 SV=1
MM1 pKa = 6.94 TGGMIVRR8 pKa = 11.84 RR9 pKa = 11.84 RR10 pKa = 11.84 RR11 pKa = 11.84 QATTGWGIGSQSAKK25 pKa = 7.28 TQPRR29 pKa = 11.84 RR30 pKa = 11.84 SRR32 pKa = 11.84 GPAGMSS38 pKa = 3.07
Molecular weight: 4.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.465
IPC2_protein 11.008
IPC_protein 12.618
Toseland 12.778
ProMoST 13.276
Dawson 12.778
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.34
Grimsley 12.822
Solomon 13.276
Lehninger 13.173
Nozaki 12.778
DTASelect 12.778
Thurlkill 12.778
EMBOSS 13.276
Sillero 12.778
Patrickios 12.106
IPC_peptide 13.276
IPC2_peptide 12.266
IPC2.peptide.svr19 9.135
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
17279
3488
20767
9582963
30
11522
461.5
51.21
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.279 ± 0.015
1.332 ± 0.008
5.79 ± 0.012
6.171 ± 0.02
3.807 ± 0.01
6.72 ± 0.018
2.411 ± 0.008
5.125 ± 0.011
4.974 ± 0.014
8.856 ± 0.02
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.277 ± 0.006
3.817 ± 0.01
5.937 ± 0.021
4.074 ± 0.012
5.893 ± 0.016
8.153 ± 0.019
6.004 ± 0.021
6.051 ± 0.013
1.522 ± 0.007
2.807 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here