Talpa europaea papillomavirus 1
Average proteome isoelectric point is 6.1
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|R9RYS7|R9RYS7_9PAPI Minor capsid protein L2 OS=Talpa europaea papillomavirus 1 OX=1338506 GN=L2 PE=3 SV=1
MM1 pKa = 7.65 IGPEE5 pKa = 4.18 PTLKK9 pKa = 10.63 DD10 pKa = 3.2 ITLDD14 pKa = 3.67 TPDD17 pKa = 5.43 SIDD20 pKa = 3.63 LNCYY24 pKa = 6.56 EE25 pKa = 4.06 TFEE28 pKa = 4.3 IEE30 pKa = 4.25 EE31 pKa = 4.46 EE32 pKa = 4.18 EE33 pKa = 4.45 VPPLGQPIKK42 pKa = 9.99 VLCYY46 pKa = 10.24 CGLCKK51 pKa = 10.06 RR52 pKa = 11.84 RR53 pKa = 11.84 IKK55 pKa = 9.8 VTVVSDD61 pKa = 3.65 RR62 pKa = 11.84 ASLISLEE69 pKa = 4.07 SLLVDD74 pKa = 3.52 SSLRR78 pKa = 11.84 FVCCSCGRR86 pKa = 11.84 RR87 pKa = 11.84 YY88 pKa = 10.45 GRR90 pKa = 4.21
Molecular weight: 10.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.759
IPC2_protein 5.003
IPC_protein 4.787
Toseland 4.635
ProMoST 4.889
Dawson 4.724
Bjellqvist 4.876
Wikipedia 4.584
Rodwell 4.622
Grimsley 4.546
Solomon 4.724
Lehninger 4.673
Nozaki 4.838
DTASelect 4.965
Thurlkill 4.635
EMBOSS 4.596
Sillero 4.889
Patrickios 3.389
IPC_peptide 4.724
IPC2_peptide 4.889
IPC2.peptide.svr19 4.895
Protein with the highest isoelectric point:
>tr|R9RZM2|R9RZM2_9PAPI Regulatory protein E2 OS=Talpa europaea papillomavirus 1 OX=1338506 GN=E2 PE=3 SV=1
MM1 pKa = 7.14 EE2 pKa = 4.63 TLKK5 pKa = 10.56 QRR7 pKa = 11.84 LDD9 pKa = 3.44 SLQNEE14 pKa = 4.42 LLSLYY19 pKa = 10.33 EE20 pKa = 4.53 KK21 pKa = 10.56 DD22 pKa = 3.79 SNDD25 pKa = 2.84 IRR27 pKa = 11.84 DD28 pKa = 4.87 QILHH32 pKa = 6.0 WNLNRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 4.03 QALLHH44 pKa = 5.75 YY45 pKa = 10.38 ARR47 pKa = 11.84 QQGLNRR53 pKa = 11.84 LGMQMVPALTVSQSRR68 pKa = 11.84 AKK70 pKa = 10.58 GAIEE74 pKa = 4.15 MEE76 pKa = 4.27 LMLTSLSTSRR86 pKa = 11.84 FGNEE90 pKa = 2.99 GWTLQEE96 pKa = 3.87 TSRR99 pKa = 11.84 EE100 pKa = 4.18 LFNTAPKK107 pKa = 9.0 HH108 pKa = 4.5 TFKK111 pKa = 10.84 KK112 pKa = 10.42 GPRR115 pKa = 11.84 HH116 pKa = 6.45 LDD118 pKa = 3.19 MIFDD122 pKa = 4.28 GNSNNTVHH130 pKa = 6.26 VPFWQYY136 pKa = 10.73 IYY138 pKa = 10.89 YY139 pKa = 9.86 QDD141 pKa = 5.49 EE142 pKa = 3.78 RR143 pKa = 11.84 DD144 pKa = 3.12 EE145 pKa = 4.22 WHH147 pKa = 7.02 RR148 pKa = 11.84 SNSDD152 pKa = 2.85 VDD154 pKa = 4.07 SVGIYY159 pKa = 10.52 YY160 pKa = 8.27 MQGTIKK166 pKa = 10.5 VYY168 pKa = 7.4 YY169 pKa = 10.05 TKK171 pKa = 10.57 FQSEE175 pKa = 3.77 ASKK178 pKa = 11.14 YY179 pKa = 10.38 GFRR182 pKa = 11.84 TWQVSHH188 pKa = 7.38 DD189 pKa = 4.07 NKK191 pKa = 10.06 PLHH194 pKa = 6.68 SSSSVGSRR202 pKa = 11.84 GPSPHH207 pKa = 6.69 SKK209 pKa = 10.68 APSTPIRR216 pKa = 11.84 RR217 pKa = 11.84 GGGRR221 pKa = 11.84 RR222 pKa = 11.84 APRR225 pKa = 11.84 RR226 pKa = 11.84 RR227 pKa = 11.84 SPSTFSRR234 pKa = 11.84 SHH236 pKa = 6.69 KK237 pKa = 9.39 EE238 pKa = 3.6 ASGSSGRR245 pKa = 11.84 GEE247 pKa = 4.43 TPPSARR253 pKa = 11.84 DD254 pKa = 3.32 VGRR257 pKa = 11.84 STEE260 pKa = 4.04 TPPRR264 pKa = 11.84 GNQSRR269 pKa = 11.84 LRR271 pKa = 11.84 SLLHH275 pKa = 6.17 EE276 pKa = 4.76 ARR278 pKa = 11.84 DD279 pKa = 3.88 PPLLVLKK286 pKa = 10.9 GPANTLKK293 pKa = 10.6 CLRR296 pKa = 11.84 YY297 pKa = 8.76 RR298 pKa = 11.84 LKK300 pKa = 11.11 GRR302 pKa = 11.84 FSHH305 pKa = 6.22 TFCNVTSTWHH315 pKa = 4.85 WTLPTGTEE323 pKa = 3.86 KK324 pKa = 10.66 CVRR327 pKa = 11.84 SCGSRR332 pKa = 11.84 MIVTFSSTEE341 pKa = 3.7 QRR343 pKa = 11.84 NLFCTKK349 pKa = 10.01 VPLPSSVTAFTGSFNGEE366 pKa = 3.63
Molecular weight: 41.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.258
IPC2_protein 9.37
IPC_protein 9.633
Toseland 10.101
ProMoST 9.823
Dawson 10.306
Bjellqvist 9.999
Wikipedia 10.482
Rodwell 10.584
Grimsley 10.379
Solomon 10.335
Lehninger 10.306
Nozaki 10.131
DTASelect 9.984
Thurlkill 10.16
EMBOSS 10.511
Sillero 10.218
Patrickios 10.116
IPC_peptide 10.335
IPC2_peptide 8.873
IPC2.peptide.svr19 8.384
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2265
90
608
323.6
36.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.901 ± 0.575
1.987 ± 0.548
5.563 ± 0.576
7.285 ± 0.591
4.812 ± 0.443
5.916 ± 0.823
1.987 ± 0.332
4.989 ± 0.745
5.21 ± 0.825
9.36 ± 0.844
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.943 ± 0.458
4.459 ± 0.717
5.916 ± 1.017
4.194 ± 0.407
6.446 ± 0.885
8.079 ± 1.282
6.313 ± 0.569
6.269 ± 0.582
1.369 ± 0.304
3.002 ± 0.265
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here