Actinomadura sp. CNU-125

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Thermomonosporaceae; Actinomadura; unclassified Actinomadura

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7656 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9SN59|A0A1Q9SN59_9ACTN Aminotransferase DegT OS=Actinomadura sp. CNU-125 OX=1904961 GN=BJF79_23095 PE=3 SV=1
MM1 pKa = 7.14IAAAAALGLTATAASAATTTVRR23 pKa = 11.84RR24 pKa = 11.84ADD26 pKa = 3.24GTAYY30 pKa = 10.21SGEE33 pKa = 3.82YY34 pKa = 10.03RR35 pKa = 11.84IVNVGNMTFADD46 pKa = 3.79STSTFTASCSDD57 pKa = 3.12ADD59 pKa = 4.3LRR61 pKa = 11.84GTINSDD67 pKa = 3.09GTGTLDD73 pKa = 3.36SASVTGCTSNLGPATVSFLNLSYY96 pKa = 11.28EE97 pKa = 4.45DD98 pKa = 3.91GQLTYY103 pKa = 10.51APSGGYY109 pKa = 10.29DD110 pKa = 3.38GTLVFSDD117 pKa = 3.42TDD119 pKa = 3.97LAVQASFLFLSCTYY133 pKa = 10.84GLDD136 pKa = 3.49ASHH139 pKa = 7.93PDD141 pKa = 3.22LTFHH145 pKa = 6.51VRR147 pKa = 11.84NPDD150 pKa = 3.14NPNNVTGEE158 pKa = 4.3FEE160 pKa = 4.55GEE162 pKa = 4.09MVDD165 pKa = 4.63ASLGLLSGDD174 pKa = 3.84AGCPTDD180 pKa = 4.05VLANGLGQANGKK192 pKa = 8.34VLPTDD197 pKa = 3.32TDD199 pKa = 3.9FLEE202 pKa = 4.63TLHH205 pKa = 6.15ITSS208 pKa = 4.07

Molecular weight: 21.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9SKY5|A0A1Q9SKY5_9ACTN Uncharacterized protein OS=Actinomadura sp. CNU-125 OX=1904961 GN=BJF79_04790 PE=4 SV=1
MM1 pKa = 7.66RR2 pKa = 11.84SAVASSGQKK11 pKa = 9.14VVRR14 pKa = 11.84PPLWSMLMIRR24 pKa = 11.84NGMLVLLTGSSPPKK38 pKa = 9.81ARR40 pKa = 11.84AAVFVQTSRR49 pKa = 11.84IFTAPRR55 pKa = 11.84WWAARR60 pKa = 11.84SSSRR64 pKa = 11.84SRR66 pKa = 11.84NRR68 pKa = 11.84SS69 pKa = 3.0

Molecular weight: 7.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7656

0

7656

2085195

29

2378

272.4

29.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.544 ± 0.042

0.837 ± 0.009

6.346 ± 0.027

5.715 ± 0.028

2.835 ± 0.018

9.636 ± 0.027

2.19 ± 0.014

3.331 ± 0.017

1.908 ± 0.02

9.945 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.923 ± 0.011

1.702 ± 0.015

6.301 ± 0.025

2.302 ± 0.016

8.957 ± 0.036

4.757 ± 0.021

5.753 ± 0.024

8.511 ± 0.029

1.537 ± 0.011

1.972 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski