Halogeometricum sp. wsp3
Average proteome isoelectric point is 7.19
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 845 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2W0FVK6|A0A2W0FVK6_9EURY Uncharacterized protein OS=Halogeometricum sp. wsp3 OX=2218585 GN=C8039_13940 PE=4 SV=1
MM1 pKa = 7.76 PSDD4 pKa = 4.17 DD5 pKa = 4.36 APPLRR10 pKa = 11.84 FEE12 pKa = 4.49 KK13 pKa = 10.79 VDD15 pKa = 3.16 GRR17 pKa = 11.84 LLVTVDD23 pKa = 3.16 GTDD26 pKa = 3.35 EE27 pKa = 4.19 VTSGSDD33 pKa = 3.54 GCCRR37 pKa = 11.84 TAAVGDD43 pKa = 3.72 PVLWGKK49 pKa = 9.41 WDD51 pKa = 3.25 IFCIVFSTYY60 pKa = 11.25 DD61 pKa = 4.3 LYY63 pKa = 11.23 CQSYY67 pKa = 9.79 SS68 pKa = 3.24
Molecular weight: 7.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.77
IPC2_protein 3.961
IPC_protein 3.897
Toseland 3.668
ProMoST 4.101
Dawson 3.91
Bjellqvist 4.075
Wikipedia 3.91
Rodwell 3.719
Grimsley 3.592
Solomon 3.897
Lehninger 3.846
Nozaki 4.05
DTASelect 4.329
Thurlkill 3.757
EMBOSS 3.91
Sillero 4.024
Patrickios 0.782
IPC_peptide 3.884
IPC2_peptide 3.986
IPC2.peptide.svr19 3.905
Protein with the highest isoelectric point:
>tr|A0A2W0FQ42|A0A2W0FQ42_9EURY Uncharacterized protein OS=Halogeometricum sp. wsp3 OX=2218585 GN=C8039_18615 PE=4 SV=1
MM1 pKa = 7.27 FAFNARR7 pKa = 11.84 TSPSRR12 pKa = 11.84 LPGRR16 pKa = 11.84 RR17 pKa = 11.84 RR18 pKa = 11.84 RR19 pKa = 3.67
Molecular weight: 2.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.43
IPC2_protein 10.921
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.974
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.149
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
845
0
845
93895
19
440
111.1
12.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.235 ± 0.129
1.631 ± 0.043
6.44 ± 0.126
5.949 ± 0.131
2.575 ± 0.06
7.378 ± 0.111
2.309 ± 0.048
3.597 ± 0.087
1.913 ± 0.068
7.449 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.004 ± 0.045
2.388 ± 0.06
5.54 ± 0.095
2.693 ± 0.062
11.541 ± 0.158
8.967 ± 0.131
7.513 ± 0.11
7.743 ± 0.103
1.159 ± 0.039
1.977 ± 0.053
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here