Bacillus phage Saddex

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Wphvirus; unclassified Wphvirus

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 208 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345AY73|A0A345AY73_9CAUD Uncharacterized protein OS=Bacillus phage Saddex OX=2282402 PE=4 SV=1
MM1 pKa = 6.89FQVRR5 pKa = 11.84DD6 pKa = 4.16VKK8 pKa = 10.99CIAYY12 pKa = 9.41INNVEE17 pKa = 4.12VGSFVIEE24 pKa = 4.39SIVGHH29 pKa = 5.81EE30 pKa = 4.1MEE32 pKa = 4.81QVLEE36 pKa = 4.3SAQWTYY42 pKa = 10.09EE43 pKa = 4.04QTWGLTNVDD52 pKa = 4.53WEE54 pKa = 4.6EE55 pKa = 3.87EE56 pKa = 4.06WW57 pKa = 3.96

Molecular weight: 6.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345AXZ9|A0A345AXZ9_9CAUD DNA-binding protein OS=Bacillus phage Saddex OX=2282402 PE=4 SV=1
MM1 pKa = 7.16TRR3 pKa = 11.84HH4 pKa = 4.53TRR6 pKa = 11.84NRR8 pKa = 11.84QRR10 pKa = 11.84RR11 pKa = 11.84LAAEE15 pKa = 4.07MEE17 pKa = 4.24TGLNHH22 pKa = 7.45DD23 pKa = 5.23FFMKK27 pKa = 10.55DD28 pKa = 2.88KK29 pKa = 10.22NLTIARR35 pKa = 11.84YY36 pKa = 9.64RR37 pKa = 11.84NALTGRR43 pKa = 11.84EE44 pKa = 3.83LDD46 pKa = 3.77VTFKK50 pKa = 9.21WVRR53 pKa = 11.84MRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84GYY59 pKa = 9.33TYY61 pKa = 11.03DD62 pKa = 3.39EE63 pKa = 4.49VNGITEE69 pKa = 4.16FMKK72 pKa = 10.4TVNTISWITVKK83 pKa = 10.77DD84 pKa = 3.74GEE86 pKa = 4.35RR87 pKa = 11.84LKK89 pKa = 11.18HH90 pKa = 6.28SMNPKK95 pKa = 9.33SGSLITYY102 pKa = 8.56SGSWGVV108 pKa = 3.17

Molecular weight: 12.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

208

0

208

41734

50

2004

200.6

22.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.192 ± 0.258

0.822 ± 0.075

6.546 ± 0.142

8.022 ± 0.321

4.002 ± 0.115

6.249 ± 0.27

1.809 ± 0.114

6.472 ± 0.139

7.857 ± 0.197

7.898 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.141 ± 0.111

5.42 ± 0.172

2.983 ± 0.159

3.314 ± 0.113

4.524 ± 0.122

5.64 ± 0.168

6.489 ± 0.248

7.097 ± 0.191

1.179 ± 0.066

4.344 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski