Bacillus phage vB_BsuM-Goe10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Spounavirinae; Okubovirus; unclassified Okubovirus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 195 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G5CH91|A0A7G5CH91_9CAUD Uncharacterized protein OS=Bacillus phage vB_BsuM-Goe10 OX=2586958 GN=Goe10_c01150 PE=4 SV=1
MM1 pKa = 7.83SDD3 pKa = 3.33NTDD6 pKa = 2.63KK7 pKa = 11.71SMTISVKK14 pKa = 10.21SHH16 pKa = 6.96DD17 pKa = 4.36GVTEE21 pKa = 4.69TITIPVEE28 pKa = 4.32EE29 pKa = 4.39YY30 pKa = 9.65QHH32 pKa = 6.89LIDD35 pKa = 4.63RR36 pKa = 11.84VSFLDD41 pKa = 3.62CLEE44 pKa = 4.31SAGVDD49 pKa = 2.91NWNGYY54 pKa = 9.9GEE56 pKa = 4.23AQEE59 pKa = 4.6MYY61 pKa = 10.59NGDD64 pKa = 3.98SEE66 pKa = 4.8EE67 pKa = 4.39GTDD70 pKa = 3.57GHH72 pKa = 7.0

Molecular weight: 7.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G5CH00|A0A7G5CH00_9CAUD Uncharacterized protein OS=Bacillus phage vB_BsuM-Goe10 OX=2586958 GN=Goe10_c00210 PE=4 SV=1
MM1 pKa = 7.32VKK3 pKa = 8.91YY4 pKa = 8.82TKK6 pKa = 9.77HH7 pKa = 6.52DD8 pKa = 3.66SYY10 pKa = 12.06AAIHH14 pKa = 5.75VNYY17 pKa = 9.75KK18 pKa = 9.63GRR20 pKa = 11.84KK21 pKa = 7.01IPKK24 pKa = 8.15GTYY27 pKa = 9.82DD28 pKa = 4.1CLVDD32 pKa = 5.91LDD34 pKa = 3.9ILDD37 pKa = 4.48WIISLGRR44 pKa = 11.84ALVIQINKK52 pKa = 7.97QGKK55 pKa = 8.61VYY57 pKa = 10.42FMYY60 pKa = 10.71KK61 pKa = 9.93KK62 pKa = 9.71GNKK65 pKa = 8.42MKK67 pKa = 9.56WLHH70 pKa = 5.69RR71 pKa = 11.84SIMGLKK77 pKa = 9.95EE78 pKa = 4.0GDD80 pKa = 3.62PAIVDD85 pKa = 4.12HH86 pKa = 6.92INRR89 pKa = 11.84NPSDD93 pKa = 3.45NRR95 pKa = 11.84RR96 pKa = 11.84CNLRR100 pKa = 11.84IATRR104 pKa = 11.84QINSRR109 pKa = 11.84NATNKK114 pKa = 9.33VSKK117 pKa = 10.47HH118 pKa = 4.51GYY120 pKa = 8.6KK121 pKa = 9.33WVAKK125 pKa = 10.02NRR127 pKa = 11.84NKK129 pKa = 10.03YY130 pKa = 8.53AANVRR135 pKa = 11.84HH136 pKa = 6.26NYY138 pKa = 8.64QKK140 pKa = 11.08VYY142 pKa = 10.21LGNYY146 pKa = 7.18DD147 pKa = 3.7TPLEE151 pKa = 4.04AHH153 pKa = 6.43RR154 pKa = 11.84VANRR158 pKa = 11.84WVHH161 pKa = 5.49EE162 pKa = 4.42NIGSEE167 pKa = 4.16FCVRR171 pKa = 11.84EE172 pKa = 4.31PIRR175 pKa = 11.84LNN177 pKa = 3.34

Molecular weight: 20.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

195

0

195

39748

35

1129

203.8

23.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.402 ± 0.193

0.941 ± 0.063

6.785 ± 0.137

8.099 ± 0.269

3.5 ± 0.124

6.755 ± 0.194

1.887 ± 0.103

6.363 ± 0.15

8.0 ± 0.252

8.066 ± 0.178

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.717 ± 0.087

5.054 ± 0.164

3.341 ± 0.141

3.376 ± 0.12

4.612 ± 0.114

6.423 ± 0.162

5.912 ± 0.181

7.346 ± 0.178

1.122 ± 0.051

4.3 ± 0.125
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski