Pseudoramibacter alactolyticus ATCC 23263

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; Pseudoramibacter; Pseudoramibacter alactolyticus

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2514 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E6MEY8|E6MEY8_9FIRM Ricin-type beta-trefoil lectin domain protein OS=Pseudoramibacter alactolyticus ATCC 23263 OX=887929 GN=HMP0721_0571 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 10.5YY3 pKa = 10.12INEE6 pKa = 3.91KK7 pKa = 9.63VAYY10 pKa = 9.96LKK12 pKa = 10.98GLSEE16 pKa = 4.04GMKK19 pKa = 10.16LAEE22 pKa = 4.78ASDD25 pKa = 3.88EE26 pKa = 4.12GKK28 pKa = 10.21MIGQLLDD35 pKa = 3.57VLEE38 pKa = 5.59DD39 pKa = 3.13ICDD42 pKa = 3.8ALDD45 pKa = 3.99GVTEE49 pKa = 4.09AHH51 pKa = 7.68DD52 pKa = 4.46DD53 pKa = 3.77LQDD56 pKa = 3.71YY57 pKa = 11.37VEE59 pKa = 5.33MIDD62 pKa = 5.94DD63 pKa = 5.28DD64 pKa = 4.35LTDD67 pKa = 4.01MEE69 pKa = 5.73DD70 pKa = 3.75FLIDD74 pKa = 3.67IADD77 pKa = 3.71EE78 pKa = 4.61NIAFEE83 pKa = 5.33DD84 pKa = 4.3EE85 pKa = 4.85DD86 pKa = 6.1DD87 pKa = 4.04EE88 pKa = 5.17TDD90 pKa = 4.88DD91 pKa = 4.28SLDD94 pKa = 3.84MIDD97 pKa = 5.52TEE99 pKa = 6.3DD100 pKa = 4.6EE101 pKa = 4.16DD102 pKa = 5.92DD103 pKa = 4.43EE104 pKa = 6.42NDD106 pKa = 4.15DD107 pKa = 3.96IYY109 pKa = 11.28EE110 pKa = 4.24VEE112 pKa = 4.71CPHH115 pKa = 6.8CGHH118 pKa = 6.81VYY120 pKa = 10.46LADD123 pKa = 3.96FEE125 pKa = 5.48SFEE128 pKa = 5.37ADD130 pKa = 3.99DD131 pKa = 5.01VICPEE136 pKa = 4.67CDD138 pKa = 2.93TKK140 pKa = 11.02FSLNEE145 pKa = 3.66EE146 pKa = 4.13TIEE149 pKa = 4.02RR150 pKa = 11.84LKK152 pKa = 10.69QAEE155 pKa = 4.61DD156 pKa = 4.06DD157 pKa = 3.76SHH159 pKa = 8.21HH160 pKa = 6.9PEE162 pKa = 4.02EE163 pKa = 4.54EE164 pKa = 4.0

Molecular weight: 18.73 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E6MK34|E6MK34_9FIRM Uncharacterized protein OS=Pseudoramibacter alactolyticus ATCC 23263 OX=887929 GN=HMP0721_2370 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 10.37KK3 pKa = 10.08IIRR6 pKa = 11.84KK7 pKa = 7.81ITTALAGFGPAAFGAAMLARR27 pKa = 11.84RR28 pKa = 11.84TRR30 pKa = 11.84FKK32 pKa = 10.72KK33 pKa = 10.62GKK35 pKa = 7.21TKK37 pKa = 10.35SRR39 pKa = 11.84RR40 pKa = 11.84RR41 pKa = 11.84PWIITTPSTT50 pKa = 3.56

Molecular weight: 5.59 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2514

0

2514

698337

37

3225

277.8

30.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.002 ± 0.066

1.406 ± 0.018

5.901 ± 0.047

5.991 ± 0.045

4.002 ± 0.037

7.548 ± 0.047

2.068 ± 0.021

6.941 ± 0.042

5.973 ± 0.044

8.874 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.097 ± 0.032

3.692 ± 0.035

3.844 ± 0.028

3.321 ± 0.028

5.528 ± 0.047

5.202 ± 0.038

5.372 ± 0.037

6.953 ± 0.043

0.917 ± 0.018

3.367 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski