Taeniopygia guttata (Zebra finch) (Poephila guttata)
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 31342 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A674HPQ7|A0A674HPQ7_TAEGU MAX dimerization protein 3 OS=Taeniopygia guttata OX=59729 GN=MXD3 PE=4 SV=1
MM1 pKa = 8.4 DD2 pKa = 3.33 YY3 pKa = 10.5 RR4 pKa = 11.84 ALVMSQVVPGQFDD17 pKa = 3.96 DD18 pKa = 4.9 ADD20 pKa = 4.53 CSDD23 pKa = 3.6 SCQLCTCEE31 pKa = 4.26 EE32 pKa = 4.28 VNAEE36 pKa = 4.42 EE37 pKa = 5.94 GDD39 pKa = 3.76 GDD41 pKa = 4.34 DD42 pKa = 5.22 NSDD45 pKa = 4.31 AEE47 pKa = 4.69 EE48 pKa = 5.61 DD49 pKa = 4.15 DD50 pKa = 5.26 EE51 pKa = 5.73 DD52 pKa = 4.47 WDD54 pKa = 3.95 WMMRR58 pKa = 11.84 WEE60 pKa = 4.7 DD61 pKa = 3.58 SRR63 pKa = 11.84 SATMLLVDD71 pKa = 6.31 AIHH74 pKa = 6.92 SMLKK78 pKa = 10.04 VILWALRR85 pKa = 11.84 VPKK88 pKa = 10.75 GNGASPGGFVCCC100 pKa = 5.17
Molecular weight: 11.1 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.742
IPC2_protein 3.694
IPC_protein 3.681
Toseland 3.465
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.643
Rodwell 3.516
Grimsley 3.376
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 4.05
Thurlkill 3.528
EMBOSS 3.643
Sillero 3.808
Patrickios 1.863
IPC_peptide 3.656
IPC2_peptide 3.783
IPC2.peptide.svr19 3.756
Protein with the highest isoelectric point:
>tr|A0A674HFJ8|A0A674HFJ8_TAEGU WD_REPEATS_REGION domain-containing protein OS=Taeniopygia guttata OX=59729 GN=SEC31A PE=3 SV=1
MM1 pKa = 7.57 ARR3 pKa = 11.84 TKK5 pKa = 9.25 QTARR9 pKa = 11.84 KK10 pKa = 7.78 STGGKK15 pKa = 9.94 APRR18 pKa = 11.84 KK19 pKa = 9.29 QLATKK24 pKa = 10.05 AARR27 pKa = 11.84 KK28 pKa = 8.72 SAPATGGVKK37 pKa = 10.03 KK38 pKa = 9.09 PHH40 pKa = 6.73 RR41 pKa = 11.84 PQVGHH46 pKa = 6.69 II47 pKa = 3.79
Molecular weight: 4.96 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.411
IPC2_protein 10.921
IPC_protein 12.442
Toseland 12.632
ProMoST 13.1
Dawson 12.632
Bjellqvist 12.618
Wikipedia 13.1
Rodwell 12.574
Grimsley 12.676
Solomon 13.115
Lehninger 13.013
Nozaki 12.632
DTASelect 12.618
Thurlkill 12.632
EMBOSS 13.13
Sillero 12.632
Patrickios 12.31
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 8.985
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
16489
14853
31342
21258392
25
7886
678.3
75.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.106 ± 0.014
2.237 ± 0.012
4.851 ± 0.009
7.199 ± 0.019
3.59 ± 0.01
6.655 ± 0.02
2.537 ± 0.006
4.429 ± 0.011
5.839 ± 0.017
9.607 ± 0.017
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.138 ± 0.005
3.743 ± 0.009
6.217 ± 0.02
4.746 ± 0.012
5.721 ± 0.014
8.303 ± 0.018
5.2 ± 0.01
6.04 ± 0.012
1.22 ± 0.005
2.621 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here