Eimeria necatrix

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Eimeriidae; Eimeria

Average proteome isoelectric point is 7.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8608 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6MDL0|U6MDL0_9EIME Uncharacterized protein OS=Eimeria necatrix OX=51315 GN=ENH_00004120 PE=4 SV=1
MM1 pKa = 7.01FQYY4 pKa = 10.91SSAAAADD11 pKa = 3.74PAAAAAAADD20 pKa = 4.07PAAAAADD27 pKa = 3.88PAAAAAAAAAAAAGPPLCSTSAVFMLSMLLFKK59 pKa = 10.14TQLQALLGVNSPASLQQQAAFAAAGNSSS87 pKa = 3.09

Molecular weight: 8.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6MUE7|U6MUE7_9EIME Uncharacterized protein OS=Eimeria necatrix OX=51315 GN=ENH_00013950 PE=4 SV=1
MM1 pKa = 7.47LNSKK5 pKa = 10.61LKK7 pKa = 9.12MPAIRR12 pKa = 11.84RR13 pKa = 11.84IMPIPKK19 pKa = 9.49SKK21 pKa = 10.67RR22 pKa = 11.84STFVAKK28 pKa = 10.43RR29 pKa = 11.84PRR31 pKa = 11.84LFLKK35 pKa = 10.69

Molecular weight: 4.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8608

0

8608

4723510

9

9857

548.7

58.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.897 ± 0.097

2.027 ± 0.018

3.376 ± 0.018

5.817 ± 0.026

2.619 ± 0.017

6.481 ± 0.035

1.968 ± 0.012

2.227 ± 0.017

3.541 ± 0.023

9.784 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.275 ± 0.009

2.055 ± 0.014

6.229 ± 0.035

10.095 ± 0.097

5.963 ± 0.023

9.536 ± 0.046

4.078 ± 0.021

4.648 ± 0.026

0.995 ± 0.008

1.39 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski