Human immunodeficiency virus 1

Taxonomy: Viruses; Riboviria; Pararnavirae; Artverviricota; Revtraviricetes; Ortervirales; Retroviridae; Orthoretrovirinae; Lentivirus

Average proteome isoelectric point is 7.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q90VU8|Q90VU8_9HIV1 Protein Vpu OS=Human immunodeficiency virus 1 OX=11676 GN=vpu PE=3 SV=1
MM1 pKa = 7.11QPIIVAIVALVVAIIIAIVVWSIVIIEE28 pKa = 3.96YY29 pKa = 10.52RR30 pKa = 11.84KK31 pKa = 9.52ILRR34 pKa = 11.84QRR36 pKa = 11.84KK37 pKa = 8.02IDD39 pKa = 3.69RR40 pKa = 11.84LIDD43 pKa = 3.54RR44 pKa = 11.84LIEE47 pKa = 4.08RR48 pKa = 11.84AEE50 pKa = 4.06DD51 pKa = 3.39SGNEE55 pKa = 4.07SEE57 pKa = 5.25GEE59 pKa = 4.06VSALVEE65 pKa = 4.12MGVEE69 pKa = 4.38MGHH72 pKa = 6.27HH73 pKa = 6.63APWDD77 pKa = 3.51IDD79 pKa = 3.74DD80 pKa = 4.17LL81 pKa = 5.12

Molecular weight: 9.14 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q90VU7|Q90VU7_9HIV1 Protein Nef OS=Human immunodeficiency virus 1 OX=11676 GN=nef PE=1 SV=1
MM1 pKa = 7.46EE2 pKa = 5.27PVDD5 pKa = 4.56PRR7 pKa = 11.84LEE9 pKa = 4.15PWKK12 pKa = 10.86HH13 pKa = 6.31PGSQPKK19 pKa = 8.02TACTNCYY26 pKa = 9.77CKK28 pKa = 10.32KK29 pKa = 10.48CCFHH33 pKa = 6.65CQVCFMTKK41 pKa = 10.46ALGISYY47 pKa = 9.84GRR49 pKa = 11.84KK50 pKa = 9.0KK51 pKa = 10.12RR52 pKa = 11.84RR53 pKa = 11.84QRR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84AHH59 pKa = 5.99QNSQTHH65 pKa = 4.76QASLSKK71 pKa = 10.76QPTSQSRR78 pKa = 11.84GDD80 pKa = 3.38PTGPKK85 pKa = 9.41EE86 pKa = 3.89

Molecular weight: 9.85 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3134

81

1003

348.2

39.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.158 ± 0.375

1.851 ± 0.337

4.116 ± 0.469

6.988 ± 0.529

2.648 ± 0.236

7.052 ± 0.261

2.425 ± 0.51

6.892 ± 0.701

6.796 ± 1.0

8.296 ± 0.5

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.914 ± 0.259

4.499 ± 0.785

5.297 ± 0.613

5.743 ± 0.509

6.158 ± 0.882

5.552 ± 0.643

5.807 ± 0.324

6.286 ± 0.513

2.904 ± 0.32

2.616 ± 0.25
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski