Olsenella sp. HMSC062G07
Average proteome isoelectric point is 6.03
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1651 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1E8VVD5|A0A1E8VVD5_9ACTN L-arabinose isomerase OS=Olsenella sp. HMSC062G07 OX=1739330 GN=HMPREF2826_04220 PE=4 SV=1
MM1 pKa = 7.31 SKK3 pKa = 10.38 VAIVYY8 pKa = 8.1 WSQTGNTQAMAKK20 pKa = 9.73 ALAEE24 pKa = 4.15 AASADD29 pKa = 3.89 VFDD32 pKa = 4.63 VADD35 pKa = 5.69 FSADD39 pKa = 3.19 MVGAYY44 pKa = 8.53 DD45 pKa = 4.85 ALAFGCPAMGSEE57 pKa = 4.28 EE58 pKa = 4.72 LDD60 pKa = 3.39 PDD62 pKa = 4.69 FEE64 pKa = 4.74 EE65 pKa = 4.68 VWDD68 pKa = 3.91 ACVPRR73 pKa = 11.84 LGQRR77 pKa = 11.84 PVALFGSYY85 pKa = 10.53 DD86 pKa = 3.48 WGCGEE91 pKa = 4.4 WMEE94 pKa = 4.48 SWVSSAEE101 pKa = 3.96 DD102 pKa = 2.99 AGVNVVGSCIVHH114 pKa = 6.55 LEE116 pKa = 4.01 PEE118 pKa = 4.49 ADD120 pKa = 3.84 DD121 pKa = 5.01 LEE123 pKa = 4.56 EE124 pKa = 4.05 LAEE127 pKa = 4.07 LAGRR131 pKa = 11.84 LAGG134 pKa = 4.12
Molecular weight: 14.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.751
IPC2_protein 3.668
IPC_protein 3.617
Toseland 3.427
ProMoST 3.783
Dawson 3.605
Bjellqvist 3.77
Wikipedia 3.541
Rodwell 3.452
Grimsley 3.338
Solomon 3.592
Lehninger 3.541
Nozaki 3.732
DTASelect 3.91
Thurlkill 3.478
EMBOSS 3.541
Sillero 3.745
Patrickios 0.846
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.708
Protein with the highest isoelectric point:
>tr|A0A1E8VXD3|A0A1E8VXD3_9ACTN Uncharacterized protein OS=Olsenella sp. HMSC062G07 OX=1739330 GN=HMPREF2826_07420 PE=4 SV=1
MM1 pKa = 7.25 TSSLILTTLSCMLGAYY17 pKa = 7.81 LLCGIPFGLIVAKK30 pKa = 10.43 GSKK33 pKa = 9.72 GHH35 pKa = 5.8 VDD37 pKa = 3.06 VRR39 pKa = 11.84 TVGSGNIGMTNVARR53 pKa = 11.84 AAGAKK58 pKa = 9.27 AAAATFLLDD67 pKa = 3.39 MGKK70 pKa = 10.43 GALSMAASRR79 pKa = 11.84 LALAHH84 pKa = 5.66 VAGVPAGEE92 pKa = 4.48 LGATGALAPLAALVFLACVCGHH114 pKa = 5.9 VFSPYY119 pKa = 10.27 LRR121 pKa = 11.84 LRR123 pKa = 11.84 GGKK126 pKa = 9.64 GISVGFGAGLVLLWPMSLAALVIFLACVVPTRR158 pKa = 11.84 YY159 pKa = 9.92 VSLGSVAAAVTMPFVGLLLGMRR181 pKa = 11.84 PPAALVVALACAVVVWRR198 pKa = 11.84 HH199 pKa = 5.46 KK200 pKa = 11.32 DD201 pKa = 3.08 NILRR205 pKa = 11.84 LRR207 pKa = 11.84 QGSEE211 pKa = 3.31 RR212 pKa = 11.84 RR213 pKa = 11.84 LTRR216 pKa = 11.84 RR217 pKa = 11.84 EE218 pKa = 3.6 GAGRR222 pKa = 11.84 RR223 pKa = 11.84 DD224 pKa = 3.58 EE225 pKa = 5.11 GEE227 pKa = 4.13 GKK229 pKa = 10.37
Molecular weight: 23.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.352
IPC2_protein 9.502
IPC_protein 10.087
Toseland 10.526
ProMoST 10.248
Dawson 10.643
Bjellqvist 10.35
Wikipedia 10.818
Rodwell 10.877
Grimsley 10.687
Solomon 10.73
Lehninger 10.701
Nozaki 10.57
DTASelect 10.321
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.584
Patrickios 10.613
IPC_peptide 10.73
IPC2_peptide 9.663
IPC2.peptide.svr19 8.493
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1651
0
1651
556233
37
1988
336.9
36.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.537 ± 0.075
1.506 ± 0.024
6.531 ± 0.055
6.066 ± 0.053
3.285 ± 0.032
8.756 ± 0.053
1.907 ± 0.024
4.302 ± 0.055
3.174 ± 0.051
9.953 ± 0.078
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.461 ± 0.031
2.423 ± 0.035
4.378 ± 0.04
2.91 ± 0.032
7.276 ± 0.075
6.059 ± 0.047
5.327 ± 0.06
8.544 ± 0.058
1.069 ± 0.022
2.535 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here