Beihai tombus-like virus 16
Average proteome isoelectric point is 7.66
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1L3KFS6|A0A1L3KFS6_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 16 OX=1922719 PE=4 SV=1
MM1 pKa = 8.13 RR2 pKa = 11.84 STKK5 pKa = 10.32 INISFKK11 pKa = 10.33 GPRR14 pKa = 11.84 MDD16 pKa = 3.95 YY17 pKa = 10.97 LYY19 pKa = 10.93 LKK21 pKa = 10.2 KK22 pKa = 10.82 LLGSNVNIQSTGNWEE37 pKa = 4.29 EE38 pKa = 3.67 MDD40 pKa = 3.73 HH41 pKa = 6.46 ARR43 pKa = 11.84 LRR45 pKa = 11.84 AFRR48 pKa = 11.84 DD49 pKa = 3.47 CCYY52 pKa = 10.6 QWLLQQCRR60 pKa = 11.84 DD61 pKa = 3.42 KK62 pKa = 10.97 RR63 pKa = 11.84 ILEE66 pKa = 4.0 IGPRR70 pKa = 11.84 RR71 pKa = 11.84 HH72 pKa = 6.35 YY73 pKa = 10.51 IPHH76 pKa = 6.3 FQQAAIKK83 pKa = 9.43 TNGKK87 pKa = 7.33 PCYY90 pKa = 8.19 YY91 pKa = 10.19 TVDD94 pKa = 3.51 KK95 pKa = 10.87 TEE97 pKa = 4.04 KK98 pKa = 10.63 ADD100 pKa = 3.7 FQGRR104 pKa = 11.84 FQDD107 pKa = 3.82 LRR109 pKa = 11.84 HH110 pKa = 6.79 DD111 pKa = 3.73 NFDD114 pKa = 3.83 IIFGVDD120 pKa = 3.24 VYY122 pKa = 11.37 DD123 pKa = 4.31 LQDD126 pKa = 4.14 FVPQNDD132 pKa = 2.9 NVTYY136 pKa = 9.76 YY137 pKa = 10.5 FALAEE142 pKa = 4.27 YY143 pKa = 9.38 PHH145 pKa = 6.37 TACGGTGKK153 pKa = 10.82 LEE155 pKa = 4.18 LPDD158 pKa = 4.14 LGVIVTPDD166 pKa = 2.84 GHH168 pKa = 5.95 TTDD171 pKa = 5.19 SGDD174 pKa = 3.41 KK175 pKa = 10.61 NYY177 pKa = 10.04 TDD179 pKa = 4.36 KK180 pKa = 11.47 VPDD183 pKa = 3.93 YY184 pKa = 9.98 LTLQLPSFAKK194 pKa = 9.74 EE195 pKa = 3.7 HH196 pKa = 6.39 WPSGWNIEE204 pKa = 4.2 LVITYY209 pKa = 9.54 GCYY212 pKa = 10.26 SIYY215 pKa = 10.19 KK216 pKa = 7.93 VHH218 pKa = 6.34 TGEE221 pKa = 4.52 RR222 pKa = 11.84 SPVSLKK228 pKa = 11.15 SNTFFDD234 pKa = 4.56 ASSSSYY240 pKa = 11.22 GYY242 pKa = 9.55 VCEE245 pKa = 4.07 PTTVGAHH252 pKa = 6.32 ICPAQLVNLAMPHH265 pKa = 5.56 GRR267 pKa = 11.84 DD268 pKa = 3.52 YY269 pKa = 11.65 NKK271 pKa = 10.27 NAIRR275 pKa = 11.84 AGTNALVDD283 pKa = 3.99 EE284 pKa = 4.71 ARR286 pKa = 11.84 KK287 pKa = 9.99 ISDD290 pKa = 4.01 DD291 pKa = 3.64 LFLTIEE297 pKa = 4.2 QIRR300 pKa = 11.84 HH301 pKa = 4.61 YY302 pKa = 9.98 ACSAVRR308 pKa = 11.84 ISSLRR313 pKa = 11.84 NAAEE317 pKa = 4.24 HH318 pKa = 6.51 ARR320 pKa = 11.84 SANDD324 pKa = 2.95 IYY326 pKa = 11.05 TALAHH331 pKa = 6.17 NARR334 pKa = 11.84 GVTVQQNASLVARR347 pKa = 11.84 LVDD350 pKa = 3.72 QIEE353 pKa = 4.19 FALEE357 pKa = 3.84 FMNGDD362 pKa = 4.0 AEE364 pKa = 4.24 QHH366 pKa = 6.55 PLTKK370 pKa = 10.58 LLTKK374 pKa = 10.23 ILTLLISTCKK384 pKa = 10.56 NIARR388 pKa = 11.84 ALRR391 pKa = 11.84 DD392 pKa = 3.86 LIEE395 pKa = 4.47 TLGDD399 pKa = 4.28 WIRR402 pKa = 11.84 EE403 pKa = 3.95 ACIWGLRR410 pKa = 11.84 QNSISLIHH418 pKa = 6.88 PLLKK422 pKa = 10.21 WILDD426 pKa = 3.72 QIHH429 pKa = 6.43 PVSYY433 pKa = 10.53 HH434 pKa = 5.72 EE435 pKa = 4.44 RR436 pKa = 11.84 DD437 pKa = 3.4 SVRR440 pKa = 11.84 SHH442 pKa = 6.84 LSSVPIAPDD451 pKa = 3.14 EE452 pKa = 4.05 TSNIHH457 pKa = 6.27 NYY459 pKa = 10.26 RR460 pKa = 11.84 NIHH463 pKa = 5.55 TEE465 pKa = 3.79 ADD467 pKa = 3.99 FKK469 pKa = 11.24 PPSSYY474 pKa = 9.65 TQLGPHH480 pKa = 5.93 LVEE483 pKa = 4.54 EE484 pKa = 4.53 RR485 pKa = 11.84 PHH487 pKa = 8.03 VIVHH491 pKa = 6.87 DD492 pKa = 3.86 KK493 pKa = 10.56 DD494 pKa = 3.68 ARR496 pKa = 11.84 DD497 pKa = 3.12 AVIIQRR503 pKa = 11.84 EE504 pKa = 4.31 GRR506 pKa = 11.84 PTPQGLDD513 pKa = 3.29 YY514 pKa = 11.2 SILEE518 pKa = 4.13 EE519 pKa = 3.79 TAEE522 pKa = 3.92 QVGIEE527 pKa = 4.15 LQSFITQEE535 pKa = 4.01 TGQSSVEE542 pKa = 3.83 PVDD545 pKa = 4.04 FQFGSTVSQARR556 pKa = 11.84 VAPSSMQPGEE566 pKa = 4.19 TSTTEE571 pKa = 3.89 QPATSPKK578 pKa = 8.91 LTSSSSSSLSNLASSRR594 pKa = 11.84 TSFLPGTFGHH604 pKa = 6.45 VPPQVGTCLPSKK616 pKa = 9.33 TLSKK620 pKa = 9.68 TIQTLSVEE628 pKa = 4.49 KK629 pKa = 10.16 PSKK632 pKa = 10.26 KK633 pKa = 8.97 QLKK636 pKa = 9.57 PFTKK640 pKa = 9.34 IAKK643 pKa = 7.65 PHH645 pKa = 6.36 KK646 pKa = 9.69 LGKK649 pKa = 8.21 STNVTSPPSTPANPPKK665 pKa = 10.25 YY666 pKa = 8.89 YY667 pKa = 10.27 KK668 pKa = 10.63 SSTTSVTEE676 pKa = 4.0 SCSDD680 pKa = 3.57 MLPSCIKK687 pKa = 10.71 LLTHH691 pKa = 5.92 SCKK694 pKa = 10.57 ASAPSPTTSEE704 pKa = 4.62 DD705 pKa = 3.14 GSLPSQRR712 pKa = 11.84 EE713 pKa = 3.76 GADD716 pKa = 3.35 AQEE719 pKa = 4.54 TPTPTSATPSSTTYY733 pKa = 8.95 YY734 pKa = 9.38 TAASRR739 pKa = 11.84 KK740 pKa = 9.05 LNPWSGGLAVMIALSSIITLMSPTLPVSALPPKK773 pKa = 10.07 SAAAAVSGTPLSVLPWFIPQQEE795 pKa = 4.36 GLHH798 pKa = 6.54 FYY800 pKa = 9.38 ATLINLLLGIVILLTIFLPKK820 pKa = 10.37 LPEE823 pKa = 3.89 FATKK827 pKa = 10.34 KK828 pKa = 10.41 NSQQKK833 pKa = 9.67 PMPTMAYY840 pKa = 9.53 QSSDD844 pKa = 3.2 VCSIHH849 pKa = 6.98 SPQNMALGFHH859 pKa = 6.67 SLQTEE864 pKa = 4.06 NPGICSYY871 pKa = 11.63 DD872 pKa = 3.28 AMEE875 pKa = 5.26 NLPTYY880 pKa = 9.8 NPPKK884 pKa = 9.83 MLVSFLKK891 pKa = 10.94 NILASHH897 pKa = 6.56 SICNKK902 pKa = 8.85 KK903 pKa = 10.63 LKK905 pKa = 10.26 IKK907 pKa = 10.73 LDD909 pKa = 3.58 DD910 pKa = 4.07 HH911 pKa = 7.3 QEE913 pKa = 3.89 LLLICHH919 pKa = 7.34 LSGSTSSPCRR929 pKa = 11.84 PSIFRR934 pKa = 11.84 GLLDD938 pKa = 4.45 NIRR941 pKa = 11.84 EE942 pKa = 4.11 FPMALLDD949 pKa = 3.81 KK950 pKa = 10.85 LFNALIKK957 pKa = 10.87 YY958 pKa = 9.92 KK959 pKa = 10.75 LEE961 pKa = 4.03 SLLFSGG967 pKa = 5.51
Molecular weight: 106.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.676
IPC2_protein 6.839
IPC_protein 6.795
Toseland 6.795
ProMoST 7.337
Dawson 7.38
Bjellqvist 7.571
Wikipedia 7.322
Rodwell 7.38
Grimsley 6.912
Solomon 7.395
Lehninger 7.41
Nozaki 7.863
DTASelect 7.644
Thurlkill 7.688
EMBOSS 7.688
Sillero 7.863
Patrickios 4.088
IPC_peptide 7.395
IPC2_peptide 7.205
IPC2.peptide.svr19 6.98
Protein with the highest isoelectric point:
>tr|A0A1L3KFS6|A0A1L3KFS6_9VIRU Uncharacterized protein OS=Beihai tombus-like virus 16 OX=1922719 PE=4 SV=1
MM1 pKa = 7.5 TLQFHH6 pKa = 7.12 LLLKK10 pKa = 9.95 IHH12 pKa = 6.52 NFLICNMKK20 pKa = 10.23 LKK22 pKa = 10.37 KK23 pKa = 10.02 KK24 pKa = 8.63 QQQRR28 pKa = 11.84 PKK30 pKa = 10.16 KK31 pKa = 9.29 APKK34 pKa = 10.06 KK35 pKa = 8.94 KK36 pKa = 7.11 TTSKK40 pKa = 10.6 PSRR43 pKa = 11.84 QLNDD47 pKa = 3.39 GLKK50 pKa = 10.15 VRR52 pKa = 11.84 YY53 pKa = 8.93 KK54 pKa = 9.31 EE55 pKa = 3.88 CWGKK59 pKa = 10.33 VEE61 pKa = 5.53 SGFNSYY67 pKa = 10.6 KK68 pKa = 10.38 FLPGSSGLKK77 pKa = 10.38 HH78 pKa = 6.83 LDD80 pKa = 3.17 SLAAIYY86 pKa = 9.77 EE87 pKa = 4.46 SYY89 pKa = 10.71 KK90 pKa = 10.12 ILSTVRR96 pKa = 11.84 VSFASSTSATTSGEE110 pKa = 3.46 IVMGVDD116 pKa = 3.04 YY117 pKa = 10.67 DD118 pKa = 3.89 ARR120 pKa = 11.84 DD121 pKa = 4.01 EE122 pKa = 4.21 ISDD125 pKa = 3.56 FHH127 pKa = 6.74 GVCALQPKK135 pKa = 9.67 HH136 pKa = 6.25 VGPVRR141 pKa = 11.84 NAGNLSVPHH150 pKa = 5.79 TRR152 pKa = 11.84 AMHH155 pKa = 5.74 KK156 pKa = 10.05 KK157 pKa = 8.25 WLFTNSSAAPGEE169 pKa = 4.04 AAFAIHH175 pKa = 6.78 VYY177 pKa = 10.6 NGAAEE182 pKa = 4.35 SAGTMWVEE190 pKa = 3.79 YY191 pKa = 9.4 DD192 pKa = 3.52 LHH194 pKa = 6.8 FISPTTKK201 pKa = 10.13 ASLVSVFEE209 pKa = 4.23 SFEE212 pKa = 4.21 VPASSGGGVANRR224 pKa = 11.84 PIYY227 pKa = 10.56 SSIRR231 pKa = 11.84 MLGNTAYY238 pKa = 10.57 NEE240 pKa = 4.1 KK241 pKa = 10.18 IAANTVLWVVSEE253 pKa = 4.38 YY254 pKa = 11.04 DD255 pKa = 3.55 VQINTIFWTILEE267 pKa = 4.17 SLTRR271 pKa = 11.84 YY272 pKa = 9.88 IEE274 pKa = 3.99 GRR276 pKa = 11.84 PYY278 pKa = 10.21 KK279 pKa = 10.47 VKK281 pKa = 10.31 AVKK284 pKa = 10.11 AIKK287 pKa = 8.3 EE288 pKa = 4.54 TQDD291 pKa = 3.26 VPATCSGSGNFWAIATSAYY310 pKa = 10.22 AAYY313 pKa = 7.81 TALIACWSVHH323 pKa = 5.91 FDD325 pKa = 4.37 LPKK328 pKa = 10.29 KK329 pKa = 10.07 DD330 pKa = 4.36 APAPTPIVSPTTLKK344 pKa = 10.9 EE345 pKa = 4.04 PFSNPSPTRR354 pKa = 11.84 EE355 pKa = 4.93 DD356 pKa = 2.89 INAQAKK362 pKa = 8.24 LQSYY366 pKa = 7.64 PKK368 pKa = 9.84 KK369 pKa = 9.72 KK370 pKa = 10.19 RR371 pKa = 11.84 EE372 pKa = 3.83 MTRR375 pKa = 11.84 AEE377 pKa = 3.88 AEE379 pKa = 3.74 EE380 pKa = 4.74 AIRR383 pKa = 11.84 KK384 pKa = 7.74 SVRR387 pKa = 11.84 DD388 pKa = 3.16 ADD390 pKa = 3.68 AARR393 pKa = 11.84 WNSIVEE399 pKa = 4.12 FFSRR403 pKa = 11.84 GNLQRR408 pKa = 11.84 TLQQDD413 pKa = 3.1 LDD415 pKa = 3.89 KK416 pKa = 11.39 AQNCPTTLIRR426 pKa = 11.84 DD427 pKa = 3.78 DD428 pKa = 3.89 PTPVLEE434 pKa = 4.25 KK435 pKa = 10.85 QEE437 pKa = 4.39 LEE439 pKa = 4.21 SPLEE443 pKa = 4.01 FTEE446 pKa = 4.97 TSCQLTIDD454 pKa = 3.87 EE455 pKa = 4.67
Molecular weight: 50.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.214
IPC2_protein 8.214
IPC_protein 8.083
Toseland 8.58
ProMoST 8.639
Dawson 9.004
Bjellqvist 8.99
Wikipedia 9.092
Rodwell 9.18
Grimsley 8.814
Solomon 9.092
Lehninger 9.077
Nozaki 9.151
DTASelect 8.843
Thurlkill 8.96
EMBOSS 9.18
Sillero 9.165
Patrickios 4.635
IPC_peptide 9.092
IPC2_peptide 7.658
IPC2.peptide.svr19 7.663
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
1744
322
967
581.3
64.74
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.683 ± 0.765
2.122 ± 0.272
4.759 ± 0.217
4.931 ± 0.446
4.014 ± 0.577
4.874 ± 0.116
2.81 ± 0.346
5.963 ± 0.254
6.135 ± 0.66
9.346 ± 0.783
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.433 ± 0.123
4.186 ± 0.124
6.25 ± 0.701
4.186 ± 0.251
4.644 ± 0.551
9.576 ± 0.551
7.454 ± 0.08
4.817 ± 0.513
1.089 ± 0.244
3.727 ± 0.38
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here