Amino acid dipepetide frequency for Staphylococcus phage IME1361_01

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.574AlaAla: 1.574 ± 0.685
0.393AlaCys: 0.393 ± 0.195
2.675AlaAsp: 2.675 ± 0.504
3.383AlaGlu: 3.383 ± 0.562
2.832AlaPhe: 2.832 ± 0.637
3.304AlaGly: 3.304 ± 0.642
0.551AlaHis: 0.551 ± 0.205
4.878AlaIle: 4.878 ± 0.737
5.114AlaLys: 5.114 ± 0.518
5.193AlaLeu: 5.193 ± 0.707
1.731AlaMet: 1.731 ± 0.459
3.226AlaAsn: 3.226 ± 0.591
1.731AlaPro: 1.731 ± 0.348
2.911AlaGln: 2.911 ± 0.606
2.203AlaArg: 2.203 ± 0.345
3.855AlaSer: 3.855 ± 0.505
3.068AlaThr: 3.068 ± 0.792
2.675AlaVal: 2.675 ± 0.578
0.708AlaTrp: 0.708 ± 0.244
2.911AlaTyr: 2.911 ± 0.396
0.0AlaXaa: 0.0 ± 0.0
Cys
0.315CysAla: 0.315 ± 0.162
0.079CysCys: 0.079 ± 0.072
0.157CysAsp: 0.157 ± 0.12
0.157CysGlu: 0.157 ± 0.122
0.236CysPhe: 0.236 ± 0.151
0.236CysGly: 0.236 ± 0.121
0.079CysHis: 0.079 ± 0.087
0.865CysIle: 0.865 ± 0.275
0.393CysLys: 0.393 ± 0.2
0.393CysLeu: 0.393 ± 0.176
0.0CysMet: 0.0 ± 0.0
0.315CysAsn: 0.315 ± 0.143
0.157CysPro: 0.157 ± 0.134
0.157CysGln: 0.157 ± 0.123
0.315CysArg: 0.315 ± 0.203
0.393CysSer: 0.393 ± 0.213
0.472CysThr: 0.472 ± 0.186
0.236CysVal: 0.236 ± 0.146
0.079CysTrp: 0.079 ± 0.073
0.315CysTyr: 0.315 ± 0.176
0.0CysXaa: 0.0 ± 0.0
Asp
3.304AspAla: 3.304 ± 0.448
0.236AspCys: 0.236 ± 0.134
4.249AspAsp: 4.249 ± 0.78
5.429AspGlu: 5.429 ± 0.92
3.226AspPhe: 3.226 ± 0.416
5.193AspGly: 5.193 ± 0.757
0.708AspHis: 0.708 ± 0.256
4.485AspIle: 4.485 ± 0.592
4.957AspLys: 4.957 ± 0.87
4.878AspLeu: 4.878 ± 0.596
1.495AspMet: 1.495 ± 0.282
4.957AspAsn: 4.957 ± 0.726
1.574AspPro: 1.574 ± 0.37
0.944AspGln: 0.944 ± 0.259
2.046AspArg: 2.046 ± 0.465
3.934AspSer: 3.934 ± 0.66
2.832AspThr: 2.832 ± 0.591
3.619AspVal: 3.619 ± 0.587
0.629AspTrp: 0.629 ± 0.223
3.383AspTyr: 3.383 ± 0.626
0.0AspXaa: 0.0 ± 0.0
Glu
4.642GluAla: 4.642 ± 0.702
0.472GluCys: 0.472 ± 0.173
4.17GluAsp: 4.17 ± 0.605
7.789GluGlu: 7.789 ± 1.02
3.619GluPhe: 3.619 ± 0.456
2.282GluGly: 2.282 ± 0.422
1.574GluHis: 1.574 ± 0.414
7.474GluIle: 7.474 ± 1.005
7.317GluLys: 7.317 ± 0.998
8.733GluLeu: 8.733 ± 1.214
2.124GluMet: 2.124 ± 0.37
5.35GluAsn: 5.35 ± 0.699
1.652GluPro: 1.652 ± 0.303
3.698GluGln: 3.698 ± 0.583
4.563GluArg: 4.563 ± 0.578
4.799GluSer: 4.799 ± 0.708
3.541GluThr: 3.541 ± 0.802
4.406GluVal: 4.406 ± 0.575
0.944GluTrp: 0.944 ± 0.256
3.934GluTyr: 3.934 ± 0.614
0.0GluXaa: 0.0 ± 0.0
Phe
2.518PheAla: 2.518 ± 0.585
0.079PheCys: 0.079 ± 0.081
2.99PheAsp: 2.99 ± 0.45
3.934PheGlu: 3.934 ± 0.489
1.338PhePhe: 1.338 ± 0.356
1.731PheGly: 1.731 ± 0.388
0.472PheHis: 0.472 ± 0.189
3.855PheIle: 3.855 ± 0.676
5.035PheLys: 5.035 ± 0.55
2.832PheLeu: 2.832 ± 0.485
1.259PheMet: 1.259 ± 0.46
4.406PheAsn: 4.406 ± 0.612
0.944PhePro: 0.944 ± 0.293
0.472PheGln: 0.472 ± 0.2
1.888PheArg: 1.888 ± 0.321
2.832PheSer: 2.832 ± 0.755
2.596PheThr: 2.596 ± 0.447
2.439PheVal: 2.439 ± 0.447
0.157PheTrp: 0.157 ± 0.131
1.495PheTyr: 1.495 ± 0.328
0.0PheXaa: 0.0 ± 0.0
Gly
2.675GlyAla: 2.675 ± 0.581
0.551GlyCys: 0.551 ± 0.23
3.619GlyAsp: 3.619 ± 0.666
3.304GlyGlu: 3.304 ± 0.701
2.518GlyPhe: 2.518 ± 0.505
3.777GlyGly: 3.777 ± 1.078
1.023GlyHis: 1.023 ± 0.235
4.249GlyIle: 4.249 ± 0.884
6.373GlyLys: 6.373 ± 0.684
5.035GlyLeu: 5.035 ± 0.926
0.708GlyMet: 0.708 ± 0.25
2.832GlyAsn: 2.832 ± 0.377
1.338GlyPro: 1.338 ± 0.354
1.495GlyGln: 1.495 ± 0.4
2.832GlyArg: 2.832 ± 0.506
2.518GlySer: 2.518 ± 0.424
2.596GlyThr: 2.596 ± 0.457
3.383GlyVal: 3.383 ± 0.651
0.944GlyTrp: 0.944 ± 0.304
2.754GlyTyr: 2.754 ± 0.473
0.0GlyXaa: 0.0 ± 0.0
His
1.023HisAla: 1.023 ± 0.383
0.079HisCys: 0.079 ± 0.09
0.944HisAsp: 0.944 ± 0.283
1.259HisGlu: 1.259 ± 0.29
1.652HisPhe: 1.652 ± 0.349
0.708HisGly: 0.708 ± 0.244
0.393HisHis: 0.393 ± 0.225
1.731HisIle: 1.731 ± 0.495
1.495HisLys: 1.495 ± 0.38
1.259HisLeu: 1.259 ± 0.29
0.157HisMet: 0.157 ± 0.126
0.944HisAsn: 0.944 ± 0.319
0.551HisPro: 0.551 ± 0.184
0.472HisGln: 0.472 ± 0.165
0.393HisArg: 0.393 ± 0.18
0.708HisSer: 0.708 ± 0.206
0.865HisThr: 0.865 ± 0.245
1.101HisVal: 1.101 ± 0.323
0.0HisTrp: 0.0 ± 0.0
1.023HisTyr: 1.023 ± 0.276
0.0HisXaa: 0.0 ± 0.0
Ile
4.799IleAla: 4.799 ± 0.975
0.315IleCys: 0.315 ± 0.164
5.822IleAsp: 5.822 ± 0.715
7.71IleGlu: 7.71 ± 0.766
2.832IlePhe: 2.832 ± 0.516
3.855IleGly: 3.855 ± 0.666
1.495IleHis: 1.495 ± 0.318
4.485IleIle: 4.485 ± 0.56
9.127IleLys: 9.127 ± 0.88
4.485IleLeu: 4.485 ± 0.471
1.574IleMet: 1.574 ± 0.304
6.609IleAsn: 6.609 ± 1.203
2.203IlePro: 2.203 ± 0.503
2.99IleGln: 2.99 ± 0.514
2.754IleArg: 2.754 ± 0.468
5.586IleSer: 5.586 ± 0.813
4.327IleThr: 4.327 ± 0.482
4.957IleVal: 4.957 ± 0.553
0.944IleTrp: 0.944 ± 0.454
2.99IleTyr: 2.99 ± 0.576
0.0IleXaa: 0.0 ± 0.0
Lys
5.193LysAla: 5.193 ± 0.667
0.157LysCys: 0.157 ± 0.131
5.665LysAsp: 5.665 ± 0.518
9.284LysGlu: 9.284 ± 1.004
3.698LysPhe: 3.698 ± 0.501
6.058LysGly: 6.058 ± 0.999
1.495LysHis: 1.495 ± 0.427
7.474LysIle: 7.474 ± 0.799
8.969LysLys: 8.969 ± 1.196
8.34LysLeu: 8.34 ± 0.932
2.439LysMet: 2.439 ± 0.45
5.98LysAsn: 5.98 ± 0.777
2.675LysPro: 2.675 ± 0.491
4.327LysGln: 4.327 ± 0.53
4.485LysArg: 4.485 ± 0.56
5.744LysSer: 5.744 ± 0.772
5.586LysThr: 5.586 ± 0.829
5.586LysVal: 5.586 ± 0.605
1.101LysTrp: 1.101 ± 0.362
4.091LysTyr: 4.091 ± 0.609
0.0LysXaa: 0.0 ± 0.0
Leu
3.541LeuAla: 3.541 ± 0.607
0.472LeuCys: 0.472 ± 0.176
5.35LeuAsp: 5.35 ± 0.656
7.553LeuGlu: 7.553 ± 0.85
3.619LeuPhe: 3.619 ± 0.465
3.541LeuGly: 3.541 ± 0.671
1.416LeuHis: 1.416 ± 0.287
5.665LeuIle: 5.665 ± 0.687
9.205LeuLys: 9.205 ± 1.042
6.845LeuLeu: 6.845 ± 0.918
1.967LeuMet: 1.967 ± 0.425
5.586LeuAsn: 5.586 ± 0.65
2.439LeuPro: 2.439 ± 0.465
3.304LeuGln: 3.304 ± 0.408
3.777LeuArg: 3.777 ± 0.575
5.114LeuSer: 5.114 ± 0.724
4.957LeuThr: 4.957 ± 0.686
3.619LeuVal: 3.619 ± 0.555
0.708LeuTrp: 0.708 ± 0.226
2.754LeuTyr: 2.754 ± 0.495
0.0LeuXaa: 0.0 ± 0.0
Met
1.101MetAla: 1.101 ± 0.346
0.079MetCys: 0.079 ± 0.078
1.338MetAsp: 1.338 ± 0.334
1.495MetGlu: 1.495 ± 0.334
0.629MetPhe: 0.629 ± 0.182
1.338MetGly: 1.338 ± 0.545
0.236MetHis: 0.236 ± 0.158
2.439MetIle: 2.439 ± 0.392
1.967MetLys: 1.967 ± 0.472
1.967MetLeu: 1.967 ± 0.321
1.023MetMet: 1.023 ± 0.286
1.574MetAsn: 1.574 ± 0.398
1.101MetPro: 1.101 ± 0.305
0.944MetGln: 0.944 ± 0.254
1.023MetArg: 1.023 ± 0.245
1.652MetSer: 1.652 ± 0.302
1.574MetThr: 1.574 ± 0.405
1.416MetVal: 1.416 ± 0.338
0.393MetTrp: 0.393 ± 0.159
0.551MetTyr: 0.551 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
4.327AsnAla: 4.327 ± 0.501
0.157AsnCys: 0.157 ± 0.122
4.642AsnAsp: 4.642 ± 0.65
5.822AsnGlu: 5.822 ± 0.972
1.81AsnPhe: 1.81 ± 0.514
5.271AsnGly: 5.271 ± 0.555
0.944AsnHis: 0.944 ± 0.309
4.013AsnIle: 4.013 ± 0.546
7.238AsnLys: 7.238 ± 1.042
4.249AsnLeu: 4.249 ± 0.396
1.338AsnMet: 1.338 ± 0.292
4.721AsnAsn: 4.721 ± 0.736
2.36AsnPro: 2.36 ± 0.365
3.304AsnGln: 3.304 ± 0.584
2.832AsnArg: 2.832 ± 0.467
3.462AsnSer: 3.462 ± 0.553
3.777AsnThr: 3.777 ± 0.392
3.619AsnVal: 3.619 ± 0.599
0.865AsnTrp: 0.865 ± 0.385
2.675AsnTyr: 2.675 ± 0.457
0.0AsnXaa: 0.0 ± 0.0
Pro
1.18ProAla: 1.18 ± 0.331
0.079ProCys: 0.079 ± 0.08
1.259ProAsp: 1.259 ± 0.28
2.36ProGlu: 2.36 ± 0.336
1.731ProPhe: 1.731 ± 0.438
1.18ProGly: 1.18 ± 0.285
0.472ProHis: 0.472 ± 0.157
2.832ProIle: 2.832 ± 0.433
2.832ProLys: 2.832 ± 0.639
1.652ProLeu: 1.652 ± 0.32
0.629ProMet: 0.629 ± 0.228
0.944ProAsn: 0.944 ± 0.294
1.023ProPro: 1.023 ± 0.254
0.629ProGln: 0.629 ± 0.207
1.338ProArg: 1.338 ± 0.329
1.731ProSer: 1.731 ± 0.423
1.416ProThr: 1.416 ± 0.348
1.652ProVal: 1.652 ± 0.296
0.157ProTrp: 0.157 ± 0.112
1.101ProTyr: 1.101 ± 0.245
0.0ProXaa: 0.0 ± 0.0
Gln
2.99GlnAla: 2.99 ± 0.486
0.393GlnCys: 0.393 ± 0.178
2.282GlnAsp: 2.282 ± 0.43
2.832GlnGlu: 2.832 ± 0.479
1.023GlnPhe: 1.023 ± 0.237
1.81GlnGly: 1.81 ± 0.45
0.629GlnHis: 0.629 ± 0.246
3.068GlnIle: 3.068 ± 0.457
3.068GlnLys: 3.068 ± 0.484
3.304GlnLeu: 3.304 ± 0.495
0.865GlnMet: 0.865 ± 0.244
2.99GlnAsn: 2.99 ± 0.457
0.944GlnPro: 0.944 ± 0.241
2.124GlnGln: 2.124 ± 0.61
1.495GlnArg: 1.495 ± 0.342
2.046GlnSer: 2.046 ± 0.471
1.81GlnThr: 1.81 ± 0.345
1.967GlnVal: 1.967 ± 0.416
0.393GlnTrp: 0.393 ± 0.159
1.574GlnTyr: 1.574 ± 0.275
0.0GlnXaa: 0.0 ± 0.0
Arg
2.36ArgAla: 2.36 ± 0.379
0.236ArgCys: 0.236 ± 0.165
2.439ArgAsp: 2.439 ± 0.465
3.855ArgGlu: 3.855 ± 0.549
1.888ArgPhe: 1.888 ± 0.354
1.652ArgGly: 1.652 ± 0.462
0.472ArgHis: 0.472 ± 0.232
3.619ArgIle: 3.619 ± 0.727
3.383ArgLys: 3.383 ± 0.649
4.17ArgLeu: 4.17 ± 0.63
1.495ArgMet: 1.495 ± 0.393
3.147ArgAsn: 3.147 ± 0.397
0.551ArgPro: 0.551 ± 0.204
1.338ArgGln: 1.338 ± 0.363
2.203ArgArg: 2.203 ± 0.422
1.416ArgSer: 1.416 ± 0.407
2.596ArgThr: 2.596 ± 0.53
2.36ArgVal: 2.36 ± 0.342
0.708ArgTrp: 0.708 ± 0.25
2.596ArgTyr: 2.596 ± 0.571
0.0ArgXaa: 0.0 ± 0.0
Ser
4.091SerAla: 4.091 ± 0.554
0.472SerCys: 0.472 ± 0.237
4.563SerAsp: 4.563 ± 0.817
5.429SerGlu: 5.429 ± 0.668
2.439SerPhe: 2.439 ± 0.568
3.226SerGly: 3.226 ± 0.66
2.046SerHis: 2.046 ± 0.396
5.35SerIle: 5.35 ± 0.831
5.114SerLys: 5.114 ± 0.678
3.619SerLeu: 3.619 ± 0.59
1.338SerMet: 1.338 ± 0.268
4.17SerAsn: 4.17 ± 0.548
1.023SerPro: 1.023 ± 0.26
1.967SerGln: 1.967 ± 0.36
1.731SerArg: 1.731 ± 0.307
2.832SerSer: 2.832 ± 0.495
3.304SerThr: 3.304 ± 0.475
2.518SerVal: 2.518 ± 0.563
0.157SerTrp: 0.157 ± 0.106
2.439SerTyr: 2.439 ± 0.598
0.0SerXaa: 0.0 ± 0.0
Thr
3.462ThrAla: 3.462 ± 0.511
0.236ThrCys: 0.236 ± 0.136
3.383ThrAsp: 3.383 ± 0.813
3.777ThrGlu: 3.777 ± 0.459
2.124ThrPhe: 2.124 ± 0.407
3.383ThrGly: 3.383 ± 0.726
1.18ThrHis: 1.18 ± 0.267
4.406ThrIle: 4.406 ± 0.486
5.35ThrLys: 5.35 ± 0.675
4.799ThrLeu: 4.799 ± 0.734
0.629ThrMet: 0.629 ± 0.193
3.068ThrAsn: 3.068 ± 0.499
2.124ThrPro: 2.124 ± 0.379
1.81ThrGln: 1.81 ± 0.362
2.203ThrArg: 2.203 ± 0.514
3.462ThrSer: 3.462 ± 0.659
3.226ThrThr: 3.226 ± 0.662
3.304ThrVal: 3.304 ± 0.424
0.787ThrTrp: 0.787 ± 0.218
2.675ThrTyr: 2.675 ± 0.475
0.0ThrXaa: 0.0 ± 0.0
Val
3.068ValAla: 3.068 ± 0.57
0.315ValCys: 0.315 ± 0.174
3.383ValAsp: 3.383 ± 0.53
2.99ValGlu: 2.99 ± 0.526
2.675ValPhe: 2.675 ± 0.54
3.304ValGly: 3.304 ± 0.505
0.944ValHis: 0.944 ± 0.253
4.17ValIle: 4.17 ± 0.579
5.665ValLys: 5.665 ± 0.669
4.957ValLeu: 4.957 ± 0.634
1.731ValMet: 1.731 ± 0.416
3.777ValAsn: 3.777 ± 0.405
1.101ValPro: 1.101 ± 0.28
2.046ValGln: 2.046 ± 0.483
2.36ValArg: 2.36 ± 0.454
2.439ValSer: 2.439 ± 0.353
3.934ValThr: 3.934 ± 0.561
3.541ValVal: 3.541 ± 0.625
0.551ValTrp: 0.551 ± 0.225
2.518ValTyr: 2.518 ± 0.384
0.0ValXaa: 0.0 ± 0.0
Trp
0.393TrpAla: 0.393 ± 0.181
0.079TrpCys: 0.079 ± 0.087
0.629TrpAsp: 0.629 ± 0.219
0.944TrpGlu: 0.944 ± 0.236
0.787TrpPhe: 0.787 ± 0.239
0.629TrpGly: 0.629 ± 0.22
0.079TrpHis: 0.079 ± 0.087
0.944TrpIle: 0.944 ± 0.226
0.944TrpLys: 0.944 ± 0.207
1.101TrpLeu: 1.101 ± 0.263
0.393TrpMet: 0.393 ± 0.178
0.708TrpAsn: 0.708 ± 0.265
0.079TrpPro: 0.079 ± 0.068
0.472TrpGln: 0.472 ± 0.193
0.393TrpArg: 0.393 ± 0.137
0.708TrpSer: 0.708 ± 0.294
0.315TrpThr: 0.315 ± 0.147
0.551TrpVal: 0.551 ± 0.21
0.079TrpTrp: 0.079 ± 0.076
0.551TrpTyr: 0.551 ± 0.204
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.36TyrAla: 2.36 ± 0.36
0.472TyrCys: 0.472 ± 0.169
2.518TyrAsp: 2.518 ± 0.609
3.777TyrGlu: 3.777 ± 0.77
2.596TyrPhe: 2.596 ± 0.554
2.124TyrGly: 2.124 ± 0.496
0.551TyrHis: 0.551 ± 0.222
3.855TyrIle: 3.855 ± 0.705
4.799TyrLys: 4.799 ± 0.628
3.619TyrLeu: 3.619 ± 0.598
0.865TyrMet: 0.865 ± 0.31
2.124TyrAsn: 2.124 ± 0.296
0.629TyrPro: 0.629 ± 0.222
2.203TyrGln: 2.203 ± 0.439
1.652TyrArg: 1.652 ± 0.406
2.596TyrSer: 2.596 ± 0.51
2.596TyrThr: 2.596 ± 0.46
2.518TyrVal: 2.518 ± 0.488
0.472TyrTrp: 0.472 ± 0.233
1.023TyrTyr: 1.023 ± 0.307
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 67 proteins (12711 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski