Xenohaliotis phage pCXc-HR2015
Average proteome isoelectric point is 6.27
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 33 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A218KSU7|A0A218KSU7_9CAUD Putative LexA family transcriptional regulator OS=Xenohaliotis phage pCXc-HR2015 OX=1933104 GN=pCXcHR2015_02 PE=4 SV=1
MM1 pKa = 7.48 SVTSQLSGIAGAVSFIEE18 pKa = 5.81 DD19 pKa = 3.56 PTAPNQIPLSTRR31 pKa = 11.84 DD32 pKa = 3.35 VSGNFYY38 pKa = 10.2 WKK40 pKa = 10.37 ASNASYY46 pKa = 10.95 PEE48 pKa = 3.9 LTGADD53 pKa = 3.07 VGKK56 pKa = 10.53 AYY58 pKa = 10.53 GIVAIDD64 pKa = 3.48 ASGAVTFGGVDD75 pKa = 3.62 AFILDD80 pKa = 3.95 DD81 pKa = 4.13 AVASIINQAGVITPPDD97 pKa = 3.55 LSVTGTSTITVGPFKK112 pKa = 10.9 AVFYY116 pKa = 8.95 EE117 pKa = 4.23 QAAGAVAPIRR127 pKa = 11.84 TIVDD131 pKa = 3.13 NASQQTITINAVGASTEE148 pKa = 4.01 DD149 pKa = 3.23 VTYY152 pKa = 11.03 LKK154 pKa = 10.51 FLSDD158 pKa = 3.41 GSFSQEE164 pKa = 4.08 TQDD167 pKa = 3.86 PSADD171 pKa = 3.5 QIVVDD176 pKa = 3.89 NYY178 pKa = 9.96 IVRR181 pKa = 11.84 VLHH184 pKa = 6.37 PGGAKK189 pKa = 9.87 IISLVPLSTIYY200 pKa = 10.71 GSPQQNLQNYY210 pKa = 7.75 AKK212 pKa = 10.73 RR213 pKa = 11.84 LGIDD217 pKa = 3.31 ANDD220 pKa = 3.09 VSFTINGTALAQSGPNARR238 pKa = 11.84 LYY240 pKa = 10.81 GYY242 pKa = 9.83 NINYY246 pKa = 9.87 SAGSDD251 pKa = 3.36 NTSVRR256 pKa = 11.84 EE257 pKa = 3.97 LPVGNIAAEE266 pKa = 4.37 YY267 pKa = 10.03 YY268 pKa = 10.84 SFDD271 pKa = 3.31 SAEE274 pKa = 3.99 RR275 pKa = 11.84 GFLFDD280 pKa = 5.23 LRR282 pKa = 11.84 RR283 pKa = 11.84 TYY285 pKa = 11.27 KK286 pKa = 10.61 LDD288 pKa = 3.92 DD289 pKa = 4.39 PSNGTSSTLANFGIMTIYY307 pKa = 10.32 ILSNGDD313 pKa = 3.6 YY314 pKa = 10.9 YY315 pKa = 11.77 LLAPQRR321 pKa = 11.84 NYY323 pKa = 7.74 TTIDD327 pKa = 3.29 SAKK330 pKa = 9.6 TDD332 pKa = 3.77 LLFYY336 pKa = 9.26 LTSIVVPEE344 pKa = 4.36 FLSKK348 pKa = 10.65 YY349 pKa = 10.22 ASLLGAVVVPATVIDD364 pKa = 4.22 ANLNTVFTANISNISLASSGGGVQTLTIDD393 pKa = 3.85 PSTGTNDD400 pKa = 4.26 DD401 pKa = 3.73 VVGTNGTNFVLRR413 pKa = 11.84 KK414 pKa = 9.38 EE415 pKa = 4.08 NAFNVTGGNEE425 pKa = 3.66 GDD427 pKa = 3.78 VLVKK431 pKa = 10.38 RR432 pKa = 11.84 GSSFAVEE439 pKa = 4.85 PIANQGVPRR448 pKa = 11.84 FNLGVNRR455 pKa = 11.84 GGFIKK460 pKa = 10.66 FNRR463 pKa = 11.84 DD464 pKa = 2.67 TGAVISSNGDD474 pKa = 2.81 ISSDD478 pKa = 3.15 FTTTVTVNLVDD489 pKa = 3.34 NAYY492 pKa = 9.33 WITFNEE498 pKa = 4.42 TGTKK502 pKa = 9.71 KK503 pKa = 10.06 PLWMSIFVQNTAGEE517 pKa = 4.19 MMPVVPNLSSNVDD530 pKa = 3.55 CLIPAFITPTEE541 pKa = 4.1 TLFLLNYY548 pKa = 10.5 LLVV551 pKa = 3.8
Molecular weight: 58.44 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.242
IPC2_protein 4.24
IPC_protein 4.24
Toseland 4.024
ProMoST 4.393
Dawson 4.228
Bjellqvist 4.38
Wikipedia 4.164
Rodwell 4.062
Grimsley 3.935
Solomon 4.228
Lehninger 4.19
Nozaki 4.342
DTASelect 4.596
Thurlkill 4.075
EMBOSS 4.164
Sillero 4.368
Patrickios 3.414
IPC_peptide 4.228
IPC2_peptide 4.342
IPC2.peptide.svr19 4.297
Protein with the highest isoelectric point:
>tr|A0A218KSX8|A0A218KSX8_9CAUD Uncharacterized protein OS=Xenohaliotis phage pCXc-HR2015 OX=1933104 GN=pCXcHR2015_31 PE=4 SV=1
MM1 pKa = 7.34 VLPIVAVGAGLFFGAGVAGVARR23 pKa = 11.84 GVARR27 pKa = 11.84 SQAASRR33 pKa = 11.84 EE34 pKa = 4.11 NASRR38 pKa = 11.84 RR39 pKa = 11.84 RR40 pKa = 11.84 LMDD43 pKa = 3.87 LQNMQIKK50 pKa = 10.1 RR51 pKa = 11.84 DD52 pKa = 3.65 ADD54 pKa = 3.96 RR55 pKa = 11.84 LRR57 pKa = 11.84 AAQLRR62 pKa = 11.84 YY63 pKa = 10.06 LDD65 pKa = 4.25 ANSSIFEE72 pKa = 4.5 TINLQSQNALDD83 pKa = 3.7 MNYY86 pKa = 9.04 IQYY89 pKa = 10.99 RR90 pKa = 11.84 MRR92 pKa = 11.84 RR93 pKa = 11.84 NNDD96 pKa = 2.76 GAGEE100 pKa = 4.07 FFGDD104 pKa = 4.47 FAQGVSRR111 pKa = 11.84 GLGYY115 pKa = 11.23 LMDD118 pKa = 3.59 VRR120 pKa = 11.84 KK121 pKa = 10.05
Molecular weight: 13.42 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.706
IPC_protein 10.789
Toseland 10.482
ProMoST 10.555
Dawson 10.672
Bjellqvist 10.496
Wikipedia 10.979
Rodwell 10.599
Grimsley 10.76
Solomon 10.833
Lehninger 10.774
Nozaki 10.452
DTASelect 10.496
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.599
Patrickios 10.292
IPC_peptide 10.833
IPC2_peptide 9.575
IPC2.peptide.svr19 8.489
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
33
0
33
10287
49
890
311.7
34.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.0 ± 0.423
0.826 ± 0.186
5.949 ± 0.287
5.356 ± 0.417
4.462 ± 0.22
6.212 ± 0.303
1.419 ± 0.215
7.339 ± 0.311
6.28 ± 0.448
8.691 ± 0.34
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.333 ± 0.235
5.842 ± 0.305
3.655 ± 0.216
3.325 ± 0.216
4.219 ± 0.344
8.039 ± 0.296
6.746 ± 0.485
6.484 ± 0.338
0.603 ± 0.07
4.219 ± 0.254
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here