Capybara microvirus Cap3_SP_423
Average proteome isoelectric point is 5.82
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4P8W8J5|A0A4P8W8J5_9VIRU Minor capsid protein OS=Capybara microvirus Cap3_SP_423 OX=2585449 PE=4 SV=1
MM1 pKa = 7.6 LFNLKK6 pKa = 10.21 YY7 pKa = 10.6 YY8 pKa = 10.02 NKK10 pKa = 10.0 CEE12 pKa = 4.09 NPEE15 pKa = 4.12 FPPLLVPQKK24 pKa = 9.51 TIVEE28 pKa = 4.22 GSEE31 pKa = 3.61 EE32 pKa = 4.59 VIFLEE37 pKa = 5.25 HH38 pKa = 6.5 VPQNDD43 pKa = 4.12 FPSEE47 pKa = 4.12 MYY49 pKa = 10.66 DD50 pKa = 3.66 LQNLKK55 pKa = 10.53 SAGISPTQFRR65 pKa = 11.84 GGSVPSTSRR74 pKa = 11.84 LSDD77 pKa = 3.19 QSKK80 pKa = 11.05 ADD82 pKa = 4.22 AIVKK86 pKa = 10.36 NIVNALNTKK95 pKa = 9.1 EE96 pKa = 4.13 NEE98 pKa = 3.98
Molecular weight: 11.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.907
IPC2_protein 4.978
IPC_protein 4.787
Toseland 4.66
ProMoST 4.736
Dawson 4.711
Bjellqvist 4.927
Wikipedia 4.558
Rodwell 4.635
Grimsley 4.584
Solomon 4.711
Lehninger 4.66
Nozaki 4.825
DTASelect 4.914
Thurlkill 4.66
EMBOSS 4.584
Sillero 4.902
Patrickios 4.075
IPC_peptide 4.724
IPC2_peptide 4.902
IPC2.peptide.svr19 4.861
Protein with the highest isoelectric point:
>tr|A0A4P8W627|A0A4P8W627_9VIRU Uncharacterized protein OS=Capybara microvirus Cap3_SP_423 OX=2585449 PE=4 SV=1
MM1 pKa = 8.06 RR2 pKa = 11.84 LRR4 pKa = 11.84 NRR6 pKa = 11.84 ITIPTAFAYY15 pKa = 10.09 QSQKK19 pKa = 10.29 RR20 pKa = 11.84 MEE22 pKa = 4.13 YY23 pKa = 9.82 TIRR26 pKa = 11.84 AYY28 pKa = 10.65 YY29 pKa = 9.75 QFEE32 pKa = 4.08 EE33 pKa = 4.59 TKK35 pKa = 10.9 LKK37 pKa = 10.77 GGQQFFYY44 pKa = 10.55 TLTYY48 pKa = 10.9 NDD50 pKa = 3.69 DD51 pKa = 3.92 HH52 pKa = 7.63 LPTFLGRR59 pKa = 11.84 NCHH62 pKa = 6.96 DD63 pKa = 4.04 YY64 pKa = 11.75 NDD66 pKa = 3.44 IRR68 pKa = 11.84 YY69 pKa = 7.86 LTVGAFAKK77 pKa = 10.56 KK78 pKa = 10.2 LLRR81 pKa = 11.84 HH82 pKa = 6.08 YY83 pKa = 9.27 NLSMKK88 pKa = 10.64 YY89 pKa = 9.21 MISSEE94 pKa = 4.06 CGEE97 pKa = 4.44 GKK99 pKa = 10.4 GKK101 pKa = 10.25 RR102 pKa = 11.84 GYY104 pKa = 9.46 GQNPHH109 pKa = 5.02 YY110 pKa = 10.6 HH111 pKa = 6.85 IIFDD115 pKa = 4.7 LRR117 pKa = 11.84 PLVPGASICPIEE129 pKa = 4.34 FRR131 pKa = 11.84 SLLRR135 pKa = 11.84 EE136 pKa = 3.68 YY137 pKa = 9.56 WQGVDD142 pKa = 4.49 EE143 pKa = 5.77 DD144 pKa = 4.52 PLAPGSKK151 pKa = 8.33 PQQYY155 pKa = 10.33 KK156 pKa = 10.53 FGIVQEE162 pKa = 4.35 GQNLGQIDD170 pKa = 3.8 NAYY173 pKa = 9.55 NASAYY178 pKa = 8.76 VSKK181 pKa = 11.09 YY182 pKa = 9.68 LVKK185 pKa = 10.22 TIDD188 pKa = 3.2 QRR190 pKa = 11.84 KK191 pKa = 7.22 EE192 pKa = 3.47 ARR194 pKa = 11.84 EE195 pKa = 3.91 VLRR198 pKa = 11.84 EE199 pKa = 4.11 LYY201 pKa = 10.41 LQVVKK206 pKa = 10.88 SFEE209 pKa = 3.88 RR210 pKa = 11.84 VKK212 pKa = 10.81 YY213 pKa = 10.98 YY214 pKa = 11.03 KK215 pKa = 10.6 DD216 pKa = 3.42 FLDD219 pKa = 3.77 NTFDD223 pKa = 3.48 EE224 pKa = 4.89 YY225 pKa = 11.59 DD226 pKa = 3.58 FRR228 pKa = 11.84 LHH230 pKa = 7.48 RR231 pKa = 11.84 YY232 pKa = 6.61 YY233 pKa = 10.67 QIYY236 pKa = 8.65 TEE238 pKa = 4.51 TNWCNSPNIPHH249 pKa = 6.72 YY250 pKa = 10.77 DD251 pKa = 3.17 AVPRR255 pKa = 11.84 DD256 pKa = 3.99 DD257 pKa = 5.03 EE258 pKa = 6.27 DD259 pKa = 3.89 FLEE262 pKa = 4.39 WLCLHH267 pKa = 6.45 HH268 pKa = 6.46 CWNRR272 pKa = 11.84 FSEE275 pKa = 4.31 WFASDD280 pKa = 2.85 INEE283 pKa = 4.36 EE284 pKa = 3.88 YY285 pKa = 10.43 RR286 pKa = 11.84 KK287 pKa = 9.98 QKK289 pKa = 10.24 NEE291 pKa = 3.33 YY292 pKa = 8.77 RR293 pKa = 11.84 NRR295 pKa = 11.84 YY296 pKa = 7.52 APKK299 pKa = 9.67 VRR301 pKa = 11.84 ISNGFGDD308 pKa = 3.89 YY309 pKa = 11.19 ALNFVDD315 pKa = 5.06 EE316 pKa = 4.43 NNKK319 pKa = 9.02 VPLMTFKK326 pKa = 10.66 GHH328 pKa = 6.09 KK329 pKa = 8.64 LKK331 pKa = 10.89 KK332 pKa = 9.67 IGSHH336 pKa = 7.0 LYY338 pKa = 10.0 RR339 pKa = 11.84 KK340 pKa = 9.75 LYY342 pKa = 9.64 KK343 pKa = 9.97 DD344 pKa = 3.38 WYY346 pKa = 9.34 VDD348 pKa = 4.3 PITKK352 pKa = 8.47 TVRR355 pKa = 11.84 YY356 pKa = 8.69 KK357 pKa = 11.12 DD358 pKa = 3.51 KK359 pKa = 11.1 DD360 pKa = 3.43 SAIIGKK366 pKa = 8.49 IQTLDD371 pKa = 3.4 DD372 pKa = 4.62 VILHH376 pKa = 5.86 KK377 pKa = 9.51 TNCVKK382 pKa = 10.91 AKK384 pKa = 9.22 LQKK387 pKa = 10.2 SSRR390 pKa = 11.84 PEE392 pKa = 3.68 LASLSNMYY400 pKa = 10.64 SIFDD404 pKa = 3.4 TVYY407 pKa = 8.55 RR408 pKa = 11.84 DD409 pKa = 3.49 RR410 pKa = 11.84 IIRR413 pKa = 11.84 LTDD416 pKa = 2.84 EE417 pKa = 4.22 RR418 pKa = 11.84 FYY420 pKa = 11.43 DD421 pKa = 4.13 DD422 pKa = 4.23 SCITPEE428 pKa = 4.07 VYY430 pKa = 9.97 QDD432 pKa = 3.48 YY433 pKa = 10.7 LYY435 pKa = 11.39 NCLIDD440 pKa = 3.86 AQNFNHH446 pKa = 5.97 FRR448 pKa = 11.84 DD449 pKa = 3.87 VTPKK453 pKa = 10.42 SDD455 pKa = 3.6 YY456 pKa = 10.5 FCQKK460 pKa = 10.11 FVDD463 pKa = 4.76 GVNPEE468 pKa = 4.16 HH469 pKa = 6.64 MPFFAPFRR477 pKa = 11.84 KK478 pKa = 10.23 DD479 pKa = 4.03 FEE481 pKa = 6.02 DD482 pKa = 3.11 ITNILDD488 pKa = 3.75 ISCAQQDD495 pKa = 3.76 QQKK498 pKa = 9.04 YY499 pKa = 7.09 VAWEE503 pKa = 4.02 KK504 pKa = 10.28 QKK506 pKa = 11.01 KK507 pKa = 9.77 VYY509 pKa = 10.46 LFQKK513 pKa = 8.35 SQNYY517 pKa = 9.54 AIQPQVLQQMRR528 pKa = 11.84 KK529 pKa = 7.13 SS530 pKa = 3.42
Molecular weight: 63.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.681
IPC2_protein 7.951
IPC_protein 7.775
Toseland 7.585
ProMoST 8.302
Dawson 8.521
Bjellqvist 8.712
Wikipedia 8.507
Rodwell 8.551
Grimsley 7.629
Solomon 8.58
Lehninger 8.565
Nozaki 8.756
DTASelect 8.521
Thurlkill 8.565
EMBOSS 8.682
Sillero 8.829
Patrickios 3.923
IPC_peptide 8.565
IPC2_peptide 7.556
IPC2.peptide.svr19 7.878
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1572
98
582
393.0
45.3
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.043 ± 0.913
1.209 ± 0.389
6.552 ± 0.783
6.17 ± 1.08
5.344 ± 1.396
5.598 ± 0.878
1.654 ± 0.403
5.725 ± 0.607
6.997 ± 0.87
8.079 ± 0.5
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.163 ± 0.289
6.87 ± 0.67
3.69 ± 0.522
5.471 ± 0.757
5.089 ± 0.516
6.743 ± 0.762
4.517 ± 0.465
5.216 ± 0.205
0.954 ± 0.146
5.916 ± 1.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here