Acromyrmex echinatior (Panamanian leafcutter ant) (Acromyrmex octospinosus echinatior)
Average proteome isoelectric point is 7.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 13962 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F4X4J0|F4X4J0_ACREC Uncharacterized protein OS=Acromyrmex echinatior OX=103372 GN=G5I_13253 PE=4 SV=1
MM1 pKa = 7.14 VVLSSLLPPQEE12 pKa = 4.66 GIRR15 pKa = 11.84 HH16 pKa = 5.66 EE17 pKa = 4.43 EE18 pKa = 4.06 PNTTVYY24 pKa = 10.78 INDD27 pKa = 3.7 RR28 pKa = 11.84 EE29 pKa = 4.35 VGKK32 pKa = 8.84 GTFYY36 pKa = 10.18 VTEE39 pKa = 4.5 SVLSWVDD46 pKa = 2.99 NDD48 pKa = 3.93 TQQGFSLEE56 pKa = 4.16 YY57 pKa = 10.02 PHH59 pKa = 7.27 ISLHH63 pKa = 6.32 AISRR67 pKa = 11.84 DD68 pKa = 3.7 EE69 pKa = 3.97 QVHH72 pKa = 5.94 PRR74 pKa = 11.84 QCLYY78 pKa = 10.91 IMIDD82 pKa = 3.5 AKK84 pKa = 11.21 VDD86 pKa = 3.92 LPDD89 pKa = 3.71 MPLPLASEE97 pKa = 4.22 NGSEE101 pKa = 4.77 NEE103 pKa = 5.14 DD104 pKa = 4.18 DD105 pKa = 4.55 NADD108 pKa = 3.68 PPITEE113 pKa = 4.18 MQFAPDD119 pKa = 3.43 NTNNLDD125 pKa = 4.13 LMFQAMSACQALHH138 pKa = 7.46 PDD140 pKa = 3.96 PQDD143 pKa = 3.33 SFSDD147 pKa = 3.31 EE148 pKa = 5.17 DD149 pKa = 3.49 IYY151 pKa = 11.58 EE152 pKa = 4.45 DD153 pKa = 4.89 AEE155 pKa = 3.9 EE156 pKa = 4.97 DD157 pKa = 3.89 YY158 pKa = 11.19 EE159 pKa = 4.62 GEE161 pKa = 3.98 YY162 pKa = 10.9 DD163 pKa = 3.93 EE164 pKa = 5.7 EE165 pKa = 4.62 VGAGDD170 pKa = 4.78 APCILPTEE178 pKa = 4.36 QMGTTHH184 pKa = 7.33 TGTEE188 pKa = 4.09 AEE190 pKa = 3.99 EE191 pKa = 4.31 AMDD194 pKa = 3.59 IEE196 pKa = 4.89 AGQFEE201 pKa = 4.86 DD202 pKa = 5.63 AEE204 pKa = 4.32 EE205 pKa = 4.4 DD206 pKa = 3.54 PP207 pKa = 5.23
Molecular weight: 23.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.771
IPC2_protein 3.63
IPC_protein 3.63
Toseland 3.414
ProMoST 3.77
Dawson 3.605
Bjellqvist 3.757
Wikipedia 3.516
Rodwell 3.452
Grimsley 3.325
Solomon 3.592
Lehninger 3.554
Nozaki 3.719
DTASelect 3.91
Thurlkill 3.465
EMBOSS 3.528
Sillero 3.745
Patrickios 0.629
IPC_peptide 3.592
IPC2_peptide 3.719
IPC2.peptide.svr19 3.681
Protein with the highest isoelectric point:
>tr|F4WZX2|F4WZX2_ACREC 39S ribosomal protein L32 mitochondrial OS=Acromyrmex echinatior OX=103372 GN=G5I_11444 PE=4 SV=1
MM1 pKa = 7.44 SAHH4 pKa = 6.1 KK5 pKa = 9.54 TFIIKK10 pKa = 10.29 RR11 pKa = 11.84 KK12 pKa = 8.65 LAKK15 pKa = 9.89 KK16 pKa = 10.19 LKK18 pKa = 8.64 QNRR21 pKa = 11.84 PIPQWVRR28 pKa = 11.84 MRR30 pKa = 11.84 TGNTIRR36 pKa = 11.84 YY37 pKa = 5.79 NAKK40 pKa = 8.47 RR41 pKa = 11.84 RR42 pKa = 11.84 HH43 pKa = 4.1 WRR45 pKa = 11.84 RR46 pKa = 11.84 TKK48 pKa = 10.89 LKK50 pKa = 10.55 LL51 pKa = 3.39
Molecular weight: 6.36 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.393
IPC2_protein 11.067
IPC_protein 12.398
Toseland 12.559
ProMoST 13.042
Dawson 12.574
Bjellqvist 12.559
Wikipedia 13.027
Rodwell 12.398
Grimsley 12.603
Solomon 13.056
Lehninger 12.954
Nozaki 12.559
DTASelect 12.559
Thurlkill 12.559
EMBOSS 13.056
Sillero 12.559
Patrickios 12.135
IPC_peptide 13.056
IPC2_peptide 12.032
IPC2.peptide.svr19 8.988
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
13962
0
13962
6253196
40
17174
447.9
50.59
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.097 ± 0.021
2.005 ± 0.028
5.417 ± 0.016
6.755 ± 0.033
3.576 ± 0.018
5.328 ± 0.026
2.525 ± 0.011
6.069 ± 0.022
6.423 ± 0.032
8.962 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.335 ± 0.012
5.139 ± 0.021
4.897 ± 0.03
4.18 ± 0.024
5.956 ± 0.025
8.144 ± 0.029
6.025 ± 0.022
5.906 ± 0.015
1.099 ± 0.008
3.135 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here