Aquamavirus A (isolate Phoca hispida/Canada/HO.02.21/2002)
Average proteome isoelectric point is 5.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A8D7N3|A8D7N3_AQUAV Capsid protein VP0 OS=Aquamavirus A (isolate Phoca hispida/Canada/HO.02.21/2002) OX=1554492 GN=SPV1gp1 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 5.2 SFVEE6 pKa = 4.58 TVTKK10 pKa = 10.27 SLPVVGPMVSAAEE23 pKa = 4.05 TAVTNMLNPAGPSAPQAISAQEE45 pKa = 3.81 QVQPQQIPEE54 pKa = 4.23 PKK56 pKa = 8.86 PVTVAMPNATYY67 pKa = 7.62 TTSSDD72 pKa = 3.33 VSFGPRR78 pKa = 11.84 PPLGSDD84 pKa = 2.72 IALSNAAAANTGALNQLKK102 pKa = 9.57 FVEE105 pKa = 4.55 VQQFVLRR112 pKa = 11.84 NNTNPFTGIASFALPKK128 pKa = 10.15 SYY130 pKa = 11.01 YY131 pKa = 9.78 SNKK134 pKa = 9.74 EE135 pKa = 3.49 FPQYY139 pKa = 10.9 GNARR143 pKa = 11.84 FFSMVRR149 pKa = 11.84 SGFVAQLQVNVAPGSSGLYY168 pKa = 9.95 LLAWCPPTQKK178 pKa = 10.69 DD179 pKa = 4.55 LSKK182 pKa = 10.92 LDD184 pKa = 3.32 YY185 pKa = 10.37 RR186 pKa = 11.84 AIGNFPSVLMDD197 pKa = 3.3 ISRR200 pKa = 11.84 MTTATLDD207 pKa = 3.04 IPYY210 pKa = 9.88 ISDD213 pKa = 3.35 TNYY216 pKa = 10.39 VKK218 pKa = 10.11 IDD220 pKa = 3.98 SDD222 pKa = 3.76 DD223 pKa = 3.1 QGYY226 pKa = 10.19 ILVVPIAPYY235 pKa = 10.37 NAGPQTDD242 pKa = 4.21 SEE244 pKa = 4.69 VLATMFVAARR254 pKa = 11.84 EE255 pKa = 4.2 GDD257 pKa = 4.08 LQCPTPYY264 pKa = 10.12 RR265 pKa = 11.84 EE266 pKa = 4.29 GFVKK270 pKa = 10.71 PEE272 pKa = 4.18 TYY274 pKa = 10.34 PIMNIQNPPGAINLASFEE292 pKa = 4.37 TALNAPSLALAGEE305 pKa = 4.16 GHH307 pKa = 7.71 LIDD310 pKa = 3.48 THH312 pKa = 5.98 TPGGYY317 pKa = 10.56 APISDD322 pKa = 5.71 LIDD325 pKa = 4.02 LSHH328 pKa = 6.89 IPSMVTNDD336 pKa = 3.18 TTDD339 pKa = 3.0 YY340 pKa = 11.13 HH341 pKa = 7.47 YY342 pKa = 11.06 FDD344 pKa = 4.07 WTDD347 pKa = 3.16 TTNKK351 pKa = 10.64 DD352 pKa = 3.46 EE353 pKa = 5.63 IIGKK357 pKa = 9.73 FNFKK361 pKa = 10.56 LNSVPNSAILANSYY375 pKa = 11.1 SFFRR379 pKa = 11.84 GPIIFHH385 pKa = 6.38 VIAAASTMHH394 pKa = 7.16 RR395 pKa = 11.84 GRR397 pKa = 11.84 LRR399 pKa = 11.84 VCFRR403 pKa = 11.84 PNSNQEE409 pKa = 3.55 YY410 pKa = 10.07 SAPQSMAVYY419 pKa = 10.15 YY420 pKa = 10.5 SLLDD424 pKa = 3.42 ISQSNCCTLAVPFNSEE440 pKa = 2.89 TWMRR444 pKa = 11.84 STDD447 pKa = 3.6 DD448 pKa = 3.19 VLGRR452 pKa = 11.84 LQIFVNNQLAANRR465 pKa = 11.84 TAANHH470 pKa = 5.8 VYY472 pKa = 10.5 VYY474 pKa = 8.67 VTASLGPDD482 pKa = 2.77 AAFYY486 pKa = 10.54 CPRR489 pKa = 11.84 SGKK492 pKa = 9.62 IEE494 pKa = 3.98 FTDD497 pKa = 3.15 QGNFEE502 pKa = 4.48 SFLRR506 pKa = 11.84 PPPPEE511 pKa = 4.0 YY512 pKa = 9.78 PVNAIPQKK520 pKa = 9.63 TEE522 pKa = 3.72 LFGEE526 pKa = 4.18 SHH528 pKa = 6.58 TLVKK532 pKa = 10.54 HH533 pKa = 5.13 LFGRR537 pKa = 11.84 FTYY540 pKa = 10.42 LGYY543 pKa = 8.83 YY544 pKa = 9.15 TPDD547 pKa = 2.9 SGGWKK552 pKa = 9.62 KK553 pKa = 10.56 VALNIPKK560 pKa = 10.15 RR561 pKa = 11.84 GVVNLMRR568 pKa = 11.84 AFCYY572 pKa = 9.68 WSGEE576 pKa = 3.87 AVLCITNNDD585 pKa = 3.91 DD586 pKa = 3.72 APVEE590 pKa = 4.12 ITHH593 pKa = 6.46 TFFDD597 pKa = 4.06 QPDD600 pKa = 3.79 EE601 pKa = 4.03 LVDD604 pKa = 3.83 MSSHH608 pKa = 6.37 GSVLVPGKK616 pKa = 10.35 SSVVTAVPFYY626 pKa = 10.88 SLTPVRR632 pKa = 11.84 SLSKK636 pKa = 9.94 PDD638 pKa = 3.71 PFGFLYY644 pKa = 10.07 IAALGTSRR652 pKa = 11.84 KK653 pKa = 9.45 VVVHH657 pKa = 5.83 LAFRR661 pKa = 11.84 NLRR664 pKa = 11.84 LYY666 pKa = 10.63 LFSGFPILDD675 pKa = 3.52 YY676 pKa = 11.49 SKK678 pKa = 10.73 LQSGCFCPLPNVYY691 pKa = 9.81 VPPTHH696 pKa = 6.76 NVLLDD701 pKa = 3.54 GDD703 pKa = 4.38 VEE705 pKa = 5.08 SNPGPLYY712 pKa = 10.81 VCSQPGKK719 pKa = 9.91 CDD721 pKa = 3.41 PFPPVLGKK729 pKa = 10.67 YY730 pKa = 9.38 YY731 pKa = 10.92 AFASNYY737 pKa = 8.76 VQDD740 pKa = 4.45 HH741 pKa = 5.73 VCSCFLPKK749 pKa = 10.46 VIVEE753 pKa = 3.94 ARR755 pKa = 11.84 VLIKK759 pKa = 7.88 PTPICFMCYY768 pKa = 9.44 FSHH771 pKa = 7.89 KK772 pKa = 10.18 IRR774 pKa = 11.84 DD775 pKa = 3.69 FTEE778 pKa = 3.41 ARR780 pKa = 11.84 IICHH784 pKa = 6.14 GSCEE788 pKa = 4.04 RR789 pKa = 11.84 HH790 pKa = 6.34 SIDD793 pKa = 4.81 YY794 pKa = 8.66 EE795 pKa = 4.39 PPCKK799 pKa = 9.72 RR800 pKa = 11.84 RR801 pKa = 11.84 LVLDD805 pKa = 3.62 VEE807 pKa = 4.73 EE808 pKa = 4.17 QGFMDD813 pKa = 3.83 VVCRR817 pKa = 11.84 MFSATYY823 pKa = 9.15 HH824 pKa = 6.52 DD825 pKa = 4.67 KK826 pKa = 11.08 VLRR829 pKa = 11.84 DD830 pKa = 3.46 HH831 pKa = 7.02 KK832 pKa = 11.14 NYY834 pKa = 10.2 IIRR837 pKa = 11.84 SCLNMLALSMSNDD850 pKa = 3.09 KK851 pKa = 10.82 CVSAIVAAAITYY863 pKa = 7.5 DD864 pKa = 3.5 TVTVVPPAEE873 pKa = 4.17 LKK875 pKa = 10.94 YY876 pKa = 10.99 LITLLVAQTYY886 pKa = 10.7 DD887 pKa = 3.28 VFVNALSLVFGRR899 pKa = 11.84 LPRR902 pKa = 11.84 NVYY905 pKa = 10.07 DD906 pKa = 3.26 VARR909 pKa = 11.84 RR910 pKa = 11.84 VFSSRR915 pKa = 11.84 PTQQGFASFTSALRR929 pKa = 11.84 ACEE932 pKa = 3.73 WWIKK936 pKa = 9.69 TIAKK940 pKa = 8.92 FVSWVKK946 pKa = 7.01 TTCFSNPLVGEE957 pKa = 4.23 ARR959 pKa = 11.84 DD960 pKa = 3.66 QLSEE964 pKa = 4.1 LLAAAEE970 pKa = 3.92 QLVLDD975 pKa = 4.44 VVNLAIPRR983 pKa = 11.84 EE984 pKa = 4.22 VIEE987 pKa = 4.21 ARR989 pKa = 11.84 RR990 pKa = 11.84 SNILDD995 pKa = 3.41 KK996 pKa = 11.24 LSYY999 pKa = 10.53 AKK1001 pKa = 10.22 KK1002 pKa = 10.06 LSVNIPEE1009 pKa = 5.24 LDD1011 pKa = 3.42 ALVNKK1016 pKa = 10.46 ASLALRR1022 pKa = 11.84 KK1023 pKa = 10.0 VPATYY1028 pKa = 8.43 VTPSHH1033 pKa = 6.65 PRR1035 pKa = 11.84 VEE1037 pKa = 4.23 PLGVWLRR1044 pKa = 11.84 GAPGSGKK1051 pKa = 10.41 SILMCKK1057 pKa = 10.09 LARR1060 pKa = 11.84 DD1061 pKa = 3.47 VSLIKK1066 pKa = 10.61 NSSVFYY1072 pKa = 10.68 HH1073 pKa = 5.53 PTGSNFFSGYY1083 pKa = 8.77 HH1084 pKa = 4.92 GQYY1087 pKa = 8.99 VHH1089 pKa = 7.3 CIDD1092 pKa = 6.05 DD1093 pKa = 4.28 LGQNKK1098 pKa = 9.79 DD1099 pKa = 3.63 EE1100 pKa = 4.99 KK1101 pKa = 10.68 DD1102 pKa = 3.0 ISLICQCISSSHH1114 pKa = 5.24 FTAPMANIEE1123 pKa = 4.47 DD1124 pKa = 4.08 KK1125 pKa = 11.39 AMQYY1129 pKa = 10.94 SSNLVIATTNMTSFRR1144 pKa = 11.84 TYY1146 pKa = 10.86 TLTTPAALEE1155 pKa = 3.82 RR1156 pKa = 11.84 RR1157 pKa = 11.84 FPLVCDD1163 pKa = 3.97 IVPKK1167 pKa = 10.0 YY1168 pKa = 10.61 SKK1170 pKa = 10.92 PNGTLNLNFDD1180 pKa = 4.3 PHH1182 pKa = 6.94 DD1183 pKa = 3.83 CWKK1186 pKa = 8.89 ITCNGQEE1193 pKa = 4.15 MCYY1196 pKa = 9.59 MDD1198 pKa = 5.47 LFNLVLEE1205 pKa = 4.54 RR1206 pKa = 11.84 LTEE1209 pKa = 4.03 RR1210 pKa = 11.84 EE1211 pKa = 4.0 KK1212 pKa = 11.23 LHH1214 pKa = 9.08 ADD1216 pKa = 3.08 MCRR1219 pKa = 11.84 QNNEE1223 pKa = 4.18 LDD1225 pKa = 3.86 DD1226 pKa = 5.68 LEE1228 pKa = 6.17 IDD1230 pKa = 3.49 DD1231 pKa = 6.23 DD1232 pKa = 3.94 IRR1234 pKa = 11.84 AKK1236 pKa = 10.43 ISLPFHH1242 pKa = 6.34 TPFDD1246 pKa = 5.66 DD1247 pKa = 3.63 IVQMQCPYY1255 pKa = 9.37 TVEE1258 pKa = 3.84 RR1259 pKa = 11.84 KK1260 pKa = 9.69 KK1261 pKa = 11.06 FYY1263 pKa = 10.8 DD1264 pKa = 3.73 RR1265 pKa = 11.84 YY1266 pKa = 10.18 LSSIKK1271 pKa = 9.92 SDD1273 pKa = 3.59 KK1274 pKa = 10.64 LSHH1277 pKa = 6.17 TATLVIGMLCGASLFTLATHH1297 pKa = 7.07 AFSFVSNCFSHH1308 pKa = 7.64 GEE1310 pKa = 4.11 DD1311 pKa = 4.06 PPGPGPDD1318 pKa = 3.22 CVRR1321 pKa = 11.84 LPEE1324 pKa = 4.18 SAYY1327 pKa = 9.97 EE1328 pKa = 4.34 GCSTRR1333 pKa = 11.84 KK1334 pKa = 7.61 TARR1337 pKa = 11.84 QLARR1341 pKa = 11.84 SVVGEE1346 pKa = 3.88 GAYY1349 pKa = 10.51 DD1350 pKa = 3.68 GNVKK1354 pKa = 9.08 RR1355 pKa = 11.84 TTARR1359 pKa = 11.84 EE1360 pKa = 4.09 LARR1363 pKa = 11.84 KK1364 pKa = 9.22 AIPSEE1369 pKa = 3.96 QGPSDD1374 pKa = 3.46 VSEE1377 pKa = 4.42 FVHH1380 pKa = 6.72 LLKK1383 pKa = 10.78 FCAYY1387 pKa = 10.15 VEE1389 pKa = 4.63 TPSGPVFGVILGGRR1403 pKa = 11.84 KK1404 pKa = 8.62 MYY1406 pKa = 10.76 FNTHH1410 pKa = 4.98 YY1411 pKa = 11.12 ASVILDD1417 pKa = 3.5 KK1418 pKa = 11.11 DD1419 pKa = 3.94 VTVCTPNKK1427 pKa = 10.3 KK1428 pKa = 10.29 YY1429 pKa = 8.5 FTRR1432 pKa = 11.84 LTKK1435 pKa = 10.51 VSSNYY1440 pKa = 9.37 DD1441 pKa = 3.39 TMIVQLDD1448 pKa = 3.83 IPEE1451 pKa = 4.76 LLPSISKK1458 pKa = 10.05 YY1459 pKa = 10.41 VSSSVPADD1467 pKa = 3.26 ALLLYY1472 pKa = 7.97 FTGKK1476 pKa = 10.3 GFYY1479 pKa = 9.31 SQSVSQVSYY1488 pKa = 10.44 LPCLEE1493 pKa = 4.16 VRR1495 pKa = 11.84 EE1496 pKa = 4.5 GMHH1499 pKa = 6.02 GASYY1503 pKa = 10.68 AYY1505 pKa = 7.13 TTRR1508 pKa = 11.84 TQKK1511 pKa = 10.82 GMCGGLLVGKK1521 pKa = 10.14 IDD1523 pKa = 4.02 GAFKK1527 pKa = 11.04 ALGFHH1532 pKa = 6.98 AAGSLEE1538 pKa = 4.01 RR1539 pKa = 11.84 GFANAFNSVPPLSTVPDD1556 pKa = 4.43 FPEE1559 pKa = 4.62 SINLSGLFSNDD1570 pKa = 2.63 VEE1572 pKa = 4.51 GVVVGQFEE1580 pKa = 4.7 GEE1582 pKa = 4.01 MLHH1585 pKa = 7.43 RR1586 pKa = 11.84 SIRR1589 pKa = 11.84 TKK1591 pKa = 10.45 FRR1593 pKa = 11.84 PTDD1596 pKa = 3.73 LQNKK1600 pKa = 8.58 IVPEE1604 pKa = 4.23 LEE1606 pKa = 3.98 PAVLSASDD1614 pKa = 3.95 PRR1616 pKa = 11.84 LKK1618 pKa = 10.9 VQIDD1622 pKa = 3.76 GDD1624 pKa = 4.38 LPDD1627 pKa = 4.75 HH1628 pKa = 6.72 LCKK1631 pKa = 10.1 KK1632 pKa = 9.11 WKK1634 pKa = 10.64 VNTKK1638 pKa = 8.65 VSRR1641 pKa = 11.84 PDD1643 pKa = 3.26 VLEE1646 pKa = 4.05 LVVNEE1651 pKa = 4.7 YY1652 pKa = 10.28 ISSLDD1657 pKa = 3.61 CEE1659 pKa = 4.25 QFEE1662 pKa = 4.24 PVTLNEE1668 pKa = 5.17 AINSSEE1674 pKa = 4.39 SPLNFNGTAGAKK1686 pKa = 8.99 YY1687 pKa = 9.52 PGMNRR1692 pKa = 11.84 RR1693 pKa = 11.84 QLLLPLNPQVRR1704 pKa = 11.84 DD1705 pKa = 3.8 DD1706 pKa = 3.99 VVKK1709 pKa = 10.75 LAGDD1713 pKa = 3.75 VGNGTATVVFEE1724 pKa = 4.4 TFMKK1728 pKa = 10.82 DD1729 pKa = 2.96 EE1730 pKa = 4.68 LRR1732 pKa = 11.84 PKK1734 pKa = 10.48 EE1735 pKa = 4.25 KK1736 pKa = 10.04 IEE1738 pKa = 4.11 SGKK1741 pKa = 8.04 TRR1743 pKa = 11.84 IVEE1746 pKa = 4.25 SCPLDD1751 pKa = 3.54 YY1752 pKa = 11.28 LLLYY1756 pKa = 10.21 RR1757 pKa = 11.84 MVMLKK1762 pKa = 10.91 SMIWWYY1768 pKa = 11.22 NSDD1771 pKa = 4.05 CIKK1774 pKa = 10.3 TGVAPGMNVYY1784 pKa = 10.14 TDD1786 pKa = 3.98 FVPMVKK1792 pKa = 9.74 QFKK1795 pKa = 9.67 KK1796 pKa = 10.38 IKK1798 pKa = 8.92 YY1799 pKa = 8.79 CLDD1802 pKa = 3.69 FSAYY1806 pKa = 10.46 DD1807 pKa = 3.44 STLSDD1812 pKa = 4.3 EE1813 pKa = 4.22 ILAAGVEE1820 pKa = 4.46 VLACTSAVPSYY1831 pKa = 10.52 VRR1833 pKa = 11.84 KK1834 pKa = 9.21 LHH1836 pKa = 6.66 APIICSHH1843 pKa = 6.39 HH1844 pKa = 4.35 WHH1846 pKa = 6.49 NNVVDD1851 pKa = 4.53 LVLGGMPSGAPCTSVLNSIVNVLMARR1877 pKa = 11.84 YY1878 pKa = 9.01 ICALMDD1884 pKa = 3.14 IDD1886 pKa = 4.36 YY1887 pKa = 9.62 PVMVAYY1893 pKa = 10.32 GDD1895 pKa = 4.29 DD1896 pKa = 3.6 NVVSFDD1902 pKa = 3.66 EE1903 pKa = 5.05 EE1904 pKa = 3.88 IDD1906 pKa = 3.4 IEE1908 pKa = 5.05 RR1909 pKa = 11.84 MVSLYY1914 pKa = 9.08 KK1915 pKa = 10.47 TEE1917 pKa = 4.56 FGVTATNHH1925 pKa = 6.82 DD1926 pKa = 4.18 KK1927 pKa = 11.1 TPVPRR1932 pKa = 11.84 PMANPVFLKK1941 pKa = 10.43 RR1942 pKa = 11.84 RR1943 pKa = 11.84 LRR1945 pKa = 11.84 FNPDD1949 pKa = 2.89 LNIQFPVLPLGEE1961 pKa = 4.79 MIDD1964 pKa = 3.71 RR1965 pKa = 11.84 MCWTRR1970 pKa = 11.84 GPEE1973 pKa = 4.0 HH1974 pKa = 7.06 LSDD1977 pKa = 3.4 QTFSFAIEE1985 pKa = 3.53 LAGYY1989 pKa = 8.18 GKK1991 pKa = 9.78 QVYY1994 pKa = 7.21 THH1996 pKa = 6.45 IRR1998 pKa = 11.84 DD1999 pKa = 3.27 AFFPYY2004 pKa = 9.87 MILPPYY2010 pKa = 10.73 SLMEE2014 pKa = 3.76 NTVRR2018 pKa = 11.84 SVCGLNPVQSFLSKK2032 pKa = 11.32 YY2033 pKa = 10.72 NFGPDD2038 pKa = 3.16 DD2039 pKa = 3.97 RR2040 pKa = 11.84 KK2041 pKa = 10.99 KK2042 pKa = 10.81 KK2043 pKa = 10.92 GIGCWPWW2050 pKa = 3.11
Molecular weight: 227.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.852
IPC2_protein 5.957
IPC_protein 6.02
Toseland 6.249
ProMoST 6.415
Dawson 6.338
Bjellqvist 6.364
Wikipedia 6.364
Rodwell 6.338
Grimsley 6.351
Solomon 6.338
Lehninger 6.338
Nozaki 6.634
DTASelect 6.795
Thurlkill 6.825
EMBOSS 6.781
Sillero 6.737
Patrickios 3.706
IPC_peptide 6.364
IPC2_peptide 6.649
IPC2.peptide.svr19 6.642
Protein with the highest isoelectric point:
>tr|A8D7N3|A8D7N3_AQUAV Capsid protein VP0 OS=Aquamavirus A (isolate Phoca hispida/Canada/HO.02.21/2002) OX=1554492 GN=SPV1gp1 PE=4 SV=1
MM1 pKa = 7.55 EE2 pKa = 5.2 SFVEE6 pKa = 4.58 TVTKK10 pKa = 10.27 SLPVVGPMVSAAEE23 pKa = 4.05 TAVTNMLNPAGPSAPQAISAQEE45 pKa = 3.81 QVQPQQIPEE54 pKa = 4.23 PKK56 pKa = 8.86 PVTVAMPNATYY67 pKa = 7.62 TTSSDD72 pKa = 3.33 VSFGPRR78 pKa = 11.84 PPLGSDD84 pKa = 2.72 IALSNAAAANTGALNQLKK102 pKa = 9.57 FVEE105 pKa = 4.55 VQQFVLRR112 pKa = 11.84 NNTNPFTGIASFALPKK128 pKa = 10.15 SYY130 pKa = 11.01 YY131 pKa = 9.78 SNKK134 pKa = 9.74 EE135 pKa = 3.49 FPQYY139 pKa = 10.9 GNARR143 pKa = 11.84 FFSMVRR149 pKa = 11.84 SGFVAQLQVNVAPGSSGLYY168 pKa = 9.95 LLAWCPPTQKK178 pKa = 10.69 DD179 pKa = 4.55 LSKK182 pKa = 10.92 LDD184 pKa = 3.32 YY185 pKa = 10.37 RR186 pKa = 11.84 AIGNFPSVLMDD197 pKa = 3.3 ISRR200 pKa = 11.84 MTTATLDD207 pKa = 3.04 IPYY210 pKa = 9.88 ISDD213 pKa = 3.35 TNYY216 pKa = 10.39 VKK218 pKa = 10.11 IDD220 pKa = 3.98 SDD222 pKa = 3.76 DD223 pKa = 3.1 QGYY226 pKa = 10.19 ILVVPIAPYY235 pKa = 10.37 NAGPQTDD242 pKa = 4.21 SEE244 pKa = 4.69 VLATMFVAARR254 pKa = 11.84 EE255 pKa = 4.2 GDD257 pKa = 4.08 LQCPTPYY264 pKa = 10.12 RR265 pKa = 11.84 EE266 pKa = 4.29 GFVKK270 pKa = 10.71 PEE272 pKa = 4.18 TYY274 pKa = 10.34 PIMNIQNPPGAINLASFEE292 pKa = 4.37 TALNAPSLALAGEE305 pKa = 4.16 GHH307 pKa = 7.71 LIDD310 pKa = 3.48 THH312 pKa = 5.98 TPGGYY317 pKa = 10.56 APISDD322 pKa = 5.71 LIDD325 pKa = 4.02 LSHH328 pKa = 6.89 IPSMVTNDD336 pKa = 3.18 TTDD339 pKa = 3.0 YY340 pKa = 11.13 HH341 pKa = 7.47 YY342 pKa = 11.06 FDD344 pKa = 4.07 WTDD347 pKa = 3.16 TTNKK351 pKa = 10.64 DD352 pKa = 3.46 EE353 pKa = 5.63 IIGKK357 pKa = 9.73 FNFKK361 pKa = 10.56 LNSVPNSAILANSYY375 pKa = 11.1 SFFRR379 pKa = 11.84 GPIIFHH385 pKa = 6.38 VIAAASTMHH394 pKa = 7.16 RR395 pKa = 11.84 GRR397 pKa = 11.84 LRR399 pKa = 11.84 VCFRR403 pKa = 11.84 PNSNQEE409 pKa = 3.55 YY410 pKa = 10.07 SAPQSMAVYY419 pKa = 10.15 YY420 pKa = 10.5 SLLDD424 pKa = 3.42 ISQSNCCTLAVPFNSEE440 pKa = 2.89 TWMRR444 pKa = 11.84 STDD447 pKa = 3.6 DD448 pKa = 3.19 VLGRR452 pKa = 11.84 LQIFVNNQLAANRR465 pKa = 11.84 TAANHH470 pKa = 5.8 VYY472 pKa = 10.5 VYY474 pKa = 8.67 VTASLGPDD482 pKa = 2.77 AAFYY486 pKa = 10.54 CPRR489 pKa = 11.84 SGKK492 pKa = 9.62 IEE494 pKa = 3.98 FTDD497 pKa = 3.15 QGNFEE502 pKa = 4.48 SFLRR506 pKa = 11.84 PPPPEE511 pKa = 4.0 YY512 pKa = 9.78 PVNAIPQKK520 pKa = 9.63 TEE522 pKa = 3.72 LFGEE526 pKa = 4.18 SHH528 pKa = 6.58 TLVKK532 pKa = 10.54 HH533 pKa = 5.13 LFGRR537 pKa = 11.84 FTYY540 pKa = 10.42 LGYY543 pKa = 8.83 YY544 pKa = 9.15 TPDD547 pKa = 2.9 SGGWKK552 pKa = 9.62 KK553 pKa = 10.56 VALNIPKK560 pKa = 10.15 RR561 pKa = 11.84 GVVNLMRR568 pKa = 11.84 AFCYY572 pKa = 9.68 WSGEE576 pKa = 3.87 AVLCITNNDD585 pKa = 3.91 DD586 pKa = 3.72 APVEE590 pKa = 4.12 ITHH593 pKa = 6.46 TFFDD597 pKa = 4.06 QPDD600 pKa = 3.79 EE601 pKa = 4.03 LVDD604 pKa = 3.83 MSSHH608 pKa = 6.37 GSVLVPGKK616 pKa = 10.35 SSVVTAVPFYY626 pKa = 10.88 SLTPVRR632 pKa = 11.84 SLSKK636 pKa = 9.94 PDD638 pKa = 3.71 PFGFLYY644 pKa = 10.07 IAALGTSRR652 pKa = 11.84 KK653 pKa = 9.45 VVVHH657 pKa = 5.83 LAFRR661 pKa = 11.84 NLRR664 pKa = 11.84 LYY666 pKa = 10.63 LFSGFPILDD675 pKa = 3.52 YY676 pKa = 11.49 SKK678 pKa = 10.73 LQSGCFCPLPNVYY691 pKa = 9.81 VPPTHH696 pKa = 6.76 NVLLDD701 pKa = 3.54 GDD703 pKa = 4.38 VEE705 pKa = 5.08 SNPGPLYY712 pKa = 10.81 VCSQPGKK719 pKa = 9.91 CDD721 pKa = 3.41 PFPPVLGKK729 pKa = 10.67 YY730 pKa = 9.38 YY731 pKa = 10.92 AFASNYY737 pKa = 8.76 VQDD740 pKa = 4.45 HH741 pKa = 5.73 VCSCFLPKK749 pKa = 10.46 VIVEE753 pKa = 3.94 ARR755 pKa = 11.84 VLIKK759 pKa = 7.88 PTPICFMCYY768 pKa = 9.44 FSHH771 pKa = 7.89 KK772 pKa = 10.18 IRR774 pKa = 11.84 DD775 pKa = 3.69 FTEE778 pKa = 3.41 ARR780 pKa = 11.84 IICHH784 pKa = 6.14 GSCEE788 pKa = 4.04 RR789 pKa = 11.84 HH790 pKa = 6.34 SIDD793 pKa = 4.81 YY794 pKa = 8.66 EE795 pKa = 4.39 PPCKK799 pKa = 9.72 RR800 pKa = 11.84 RR801 pKa = 11.84 LVLDD805 pKa = 3.62 VEE807 pKa = 4.73 EE808 pKa = 4.17 QGFMDD813 pKa = 3.83 VVCRR817 pKa = 11.84 MFSATYY823 pKa = 9.15 HH824 pKa = 6.52 DD825 pKa = 4.67 KK826 pKa = 11.08 VLRR829 pKa = 11.84 DD830 pKa = 3.46 HH831 pKa = 7.02 KK832 pKa = 11.14 NYY834 pKa = 10.2 IIRR837 pKa = 11.84 SCLNMLALSMSNDD850 pKa = 3.09 KK851 pKa = 10.82 CVSAIVAAAITYY863 pKa = 7.5 DD864 pKa = 3.5 TVTVVPPAEE873 pKa = 4.17 LKK875 pKa = 10.94 YY876 pKa = 10.99 LITLLVAQTYY886 pKa = 10.7 DD887 pKa = 3.28 VFVNALSLVFGRR899 pKa = 11.84 LPRR902 pKa = 11.84 NVYY905 pKa = 10.07 DD906 pKa = 3.26 VARR909 pKa = 11.84 RR910 pKa = 11.84 VFSSRR915 pKa = 11.84 PTQQGFASFTSALRR929 pKa = 11.84 ACEE932 pKa = 3.73 WWIKK936 pKa = 9.69 TIAKK940 pKa = 8.92 FVSWVKK946 pKa = 7.01 TTCFSNPLVGEE957 pKa = 4.23 ARR959 pKa = 11.84 DD960 pKa = 3.66 QLSEE964 pKa = 4.1 LLAAAEE970 pKa = 3.92 QLVLDD975 pKa = 4.44 VVNLAIPRR983 pKa = 11.84 EE984 pKa = 4.22 VIEE987 pKa = 4.21 ARR989 pKa = 11.84 RR990 pKa = 11.84 SNILDD995 pKa = 3.41 KK996 pKa = 11.24 LSYY999 pKa = 10.53 AKK1001 pKa = 10.22 KK1002 pKa = 10.06 LSVNIPEE1009 pKa = 5.24 LDD1011 pKa = 3.42 ALVNKK1016 pKa = 10.46 ASLALRR1022 pKa = 11.84 KK1023 pKa = 10.0 VPATYY1028 pKa = 8.43 VTPSHH1033 pKa = 6.65 PRR1035 pKa = 11.84 VEE1037 pKa = 4.23 PLGVWLRR1044 pKa = 11.84 GAPGSGKK1051 pKa = 10.41 SILMCKK1057 pKa = 10.09 LARR1060 pKa = 11.84 DD1061 pKa = 3.47 VSLIKK1066 pKa = 10.61 NSSVFYY1072 pKa = 10.68 HH1073 pKa = 5.53 PTGSNFFSGYY1083 pKa = 8.77 HH1084 pKa = 4.92 GQYY1087 pKa = 8.99 VHH1089 pKa = 7.3 CIDD1092 pKa = 6.05 DD1093 pKa = 4.28 LGQNKK1098 pKa = 9.79 DD1099 pKa = 3.63 EE1100 pKa = 4.99 KK1101 pKa = 10.68 DD1102 pKa = 3.0 ISLICQCISSSHH1114 pKa = 5.24 FTAPMANIEE1123 pKa = 4.47 DD1124 pKa = 4.08 KK1125 pKa = 11.39 AMQYY1129 pKa = 10.94 SSNLVIATTNMTSFRR1144 pKa = 11.84 TYY1146 pKa = 10.86 TLTTPAALEE1155 pKa = 3.82 RR1156 pKa = 11.84 RR1157 pKa = 11.84 FPLVCDD1163 pKa = 3.97 IVPKK1167 pKa = 10.0 YY1168 pKa = 10.61 SKK1170 pKa = 10.92 PNGTLNLNFDD1180 pKa = 4.3 PHH1182 pKa = 6.94 DD1183 pKa = 3.83 CWKK1186 pKa = 8.89 ITCNGQEE1193 pKa = 4.15 MCYY1196 pKa = 9.59 MDD1198 pKa = 5.47 LFNLVLEE1205 pKa = 4.54 RR1206 pKa = 11.84 LTEE1209 pKa = 4.03 RR1210 pKa = 11.84 EE1211 pKa = 4.0 KK1212 pKa = 11.23 LHH1214 pKa = 9.08 ADD1216 pKa = 3.08 MCRR1219 pKa = 11.84 QNNEE1223 pKa = 4.18 LDD1225 pKa = 3.86 DD1226 pKa = 5.68 LEE1228 pKa = 6.17 IDD1230 pKa = 3.49 DD1231 pKa = 6.23 DD1232 pKa = 3.94 IRR1234 pKa = 11.84 AKK1236 pKa = 10.43 ISLPFHH1242 pKa = 6.34 TPFDD1246 pKa = 5.66 DD1247 pKa = 3.63 IVQMQCPYY1255 pKa = 9.37 TVEE1258 pKa = 3.84 RR1259 pKa = 11.84 KK1260 pKa = 9.69 KK1261 pKa = 11.06 FYY1263 pKa = 10.8 DD1264 pKa = 3.73 RR1265 pKa = 11.84 YY1266 pKa = 10.18 LSSIKK1271 pKa = 9.92 SDD1273 pKa = 3.59 KK1274 pKa = 10.64 LSHH1277 pKa = 6.17 TATLVIGMLCGASLFTLATHH1297 pKa = 7.07 AFSFVSNCFSHH1308 pKa = 7.64 GEE1310 pKa = 4.11 DD1311 pKa = 4.06 PPGPGPDD1318 pKa = 3.22 CVRR1321 pKa = 11.84 LPEE1324 pKa = 4.18 SAYY1327 pKa = 9.97 EE1328 pKa = 4.34 GCSTRR1333 pKa = 11.84 KK1334 pKa = 7.61 TARR1337 pKa = 11.84 QLARR1341 pKa = 11.84 SVVGEE1346 pKa = 3.88 GAYY1349 pKa = 10.51 DD1350 pKa = 3.68 GNVKK1354 pKa = 9.08 RR1355 pKa = 11.84 TTARR1359 pKa = 11.84 EE1360 pKa = 4.09 LARR1363 pKa = 11.84 KK1364 pKa = 9.22 AIPSEE1369 pKa = 3.96 QGPSDD1374 pKa = 3.46 VSEE1377 pKa = 4.42 FVHH1380 pKa = 6.72 LLKK1383 pKa = 10.78 FCAYY1387 pKa = 10.15 VEE1389 pKa = 4.63 TPSGPVFGVILGGRR1403 pKa = 11.84 KK1404 pKa = 8.62 MYY1406 pKa = 10.76 FNTHH1410 pKa = 4.98 YY1411 pKa = 11.12 ASVILDD1417 pKa = 3.5 KK1418 pKa = 11.11 DD1419 pKa = 3.94 VTVCTPNKK1427 pKa = 10.3 KK1428 pKa = 10.29 YY1429 pKa = 8.5 FTRR1432 pKa = 11.84 LTKK1435 pKa = 10.51 VSSNYY1440 pKa = 9.37 DD1441 pKa = 3.39 TMIVQLDD1448 pKa = 3.83 IPEE1451 pKa = 4.76 LLPSISKK1458 pKa = 10.05 YY1459 pKa = 10.41 VSSSVPADD1467 pKa = 3.26 ALLLYY1472 pKa = 7.97 FTGKK1476 pKa = 10.3 GFYY1479 pKa = 9.31 SQSVSQVSYY1488 pKa = 10.44 LPCLEE1493 pKa = 4.16 VRR1495 pKa = 11.84 EE1496 pKa = 4.5 GMHH1499 pKa = 6.02 GASYY1503 pKa = 10.68 AYY1505 pKa = 7.13 TTRR1508 pKa = 11.84 TQKK1511 pKa = 10.82 GMCGGLLVGKK1521 pKa = 10.14 IDD1523 pKa = 4.02 GAFKK1527 pKa = 11.04 ALGFHH1532 pKa = 6.98 AAGSLEE1538 pKa = 4.01 RR1539 pKa = 11.84 GFANAFNSVPPLSTVPDD1556 pKa = 4.43 FPEE1559 pKa = 4.62 SINLSGLFSNDD1570 pKa = 2.63 VEE1572 pKa = 4.51 GVVVGQFEE1580 pKa = 4.7 GEE1582 pKa = 4.01 MLHH1585 pKa = 7.43 RR1586 pKa = 11.84 SIRR1589 pKa = 11.84 TKK1591 pKa = 10.45 FRR1593 pKa = 11.84 PTDD1596 pKa = 3.73 LQNKK1600 pKa = 8.58 IVPEE1604 pKa = 4.23 LEE1606 pKa = 3.98 PAVLSASDD1614 pKa = 3.95 PRR1616 pKa = 11.84 LKK1618 pKa = 10.9 VQIDD1622 pKa = 3.76 GDD1624 pKa = 4.38 LPDD1627 pKa = 4.75 HH1628 pKa = 6.72 LCKK1631 pKa = 10.1 KK1632 pKa = 9.11 WKK1634 pKa = 10.64 VNTKK1638 pKa = 8.65 VSRR1641 pKa = 11.84 PDD1643 pKa = 3.26 VLEE1646 pKa = 4.05 LVVNEE1651 pKa = 4.7 YY1652 pKa = 10.28 ISSLDD1657 pKa = 3.61 CEE1659 pKa = 4.25 QFEE1662 pKa = 4.24 PVTLNEE1668 pKa = 5.17 AINSSEE1674 pKa = 4.39 SPLNFNGTAGAKK1686 pKa = 8.99 YY1687 pKa = 9.52 PGMNRR1692 pKa = 11.84 RR1693 pKa = 11.84 QLLLPLNPQVRR1704 pKa = 11.84 DD1705 pKa = 3.8 DD1706 pKa = 3.99 VVKK1709 pKa = 10.75 LAGDD1713 pKa = 3.75 VGNGTATVVFEE1724 pKa = 4.4 TFMKK1728 pKa = 10.82 DD1729 pKa = 2.96 EE1730 pKa = 4.68 LRR1732 pKa = 11.84 PKK1734 pKa = 10.48 EE1735 pKa = 4.25 KK1736 pKa = 10.04 IEE1738 pKa = 4.11 SGKK1741 pKa = 8.04 TRR1743 pKa = 11.84 IVEE1746 pKa = 4.25 SCPLDD1751 pKa = 3.54 YY1752 pKa = 11.28 LLLYY1756 pKa = 10.21 RR1757 pKa = 11.84 MVMLKK1762 pKa = 10.91 SMIWWYY1768 pKa = 11.22 NSDD1771 pKa = 4.05 CIKK1774 pKa = 10.3 TGVAPGMNVYY1784 pKa = 10.14 TDD1786 pKa = 3.98 FVPMVKK1792 pKa = 9.74 QFKK1795 pKa = 9.67 KK1796 pKa = 10.38 IKK1798 pKa = 8.92 YY1799 pKa = 8.79 CLDD1802 pKa = 3.69 FSAYY1806 pKa = 10.46 DD1807 pKa = 3.44 STLSDD1812 pKa = 4.3 EE1813 pKa = 4.22 ILAAGVEE1820 pKa = 4.46 VLACTSAVPSYY1831 pKa = 10.52 VRR1833 pKa = 11.84 KK1834 pKa = 9.21 LHH1836 pKa = 6.66 APIICSHH1843 pKa = 6.39 HH1844 pKa = 4.35 WHH1846 pKa = 6.49 NNVVDD1851 pKa = 4.53 LVLGGMPSGAPCTSVLNSIVNVLMARR1877 pKa = 11.84 YY1878 pKa = 9.01 ICALMDD1884 pKa = 3.14 IDD1886 pKa = 4.36 YY1887 pKa = 9.62 PVMVAYY1893 pKa = 10.32 GDD1895 pKa = 4.29 DD1896 pKa = 3.6 NVVSFDD1902 pKa = 3.66 EE1903 pKa = 5.05 EE1904 pKa = 3.88 IDD1906 pKa = 3.4 IEE1908 pKa = 5.05 RR1909 pKa = 11.84 MVSLYY1914 pKa = 9.08 KK1915 pKa = 10.47 TEE1917 pKa = 4.56 FGVTATNHH1925 pKa = 6.82 DD1926 pKa = 4.18 KK1927 pKa = 11.1 TPVPRR1932 pKa = 11.84 PMANPVFLKK1941 pKa = 10.43 RR1942 pKa = 11.84 RR1943 pKa = 11.84 LRR1945 pKa = 11.84 FNPDD1949 pKa = 2.89 LNIQFPVLPLGEE1961 pKa = 4.79 MIDD1964 pKa = 3.71 RR1965 pKa = 11.84 MCWTRR1970 pKa = 11.84 GPEE1973 pKa = 4.0 HH1974 pKa = 7.06 LSDD1977 pKa = 3.4 QTFSFAIEE1985 pKa = 3.53 LAGYY1989 pKa = 8.18 GKK1991 pKa = 9.78 QVYY1994 pKa = 7.21 THH1996 pKa = 6.45 IRR1998 pKa = 11.84 DD1999 pKa = 3.27 AFFPYY2004 pKa = 9.87 MILPPYY2010 pKa = 10.73 SLMEE2014 pKa = 3.76 NTVRR2018 pKa = 11.84 SVCGLNPVQSFLSKK2032 pKa = 11.32 YY2033 pKa = 10.72 NFGPDD2038 pKa = 3.16 DD2039 pKa = 3.97 RR2040 pKa = 11.84 KK2041 pKa = 10.99 KK2042 pKa = 10.81 KK2043 pKa = 10.92 GIGCWPWW2050 pKa = 3.11
Molecular weight: 227.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.852
IPC2_protein 5.957
IPC_protein 6.02
Toseland 6.249
ProMoST 6.415
Dawson 6.338
Bjellqvist 6.364
Wikipedia 6.364
Rodwell 6.338
Grimsley 6.351
Solomon 6.338
Lehninger 6.338
Nozaki 6.634
DTASelect 6.795
Thurlkill 6.825
EMBOSS 6.781
Sillero 6.737
Patrickios 3.706
IPC_peptide 6.364
IPC2_peptide 6.649
IPC2.peptide.svr19 6.642
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1
0
1
2050
2050
2050
2050.0
227.65
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.024 ± 0.0
2.634 ± 0.0
5.561 ± 0.0
4.341 ± 0.0
5.024 ± 0.0
5.512 ± 0.0
2.098 ± 0.0
4.829 ± 0.0
4.732 ± 0.0
9.073 ± 0.0
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.488 ± 0.0
4.878 ± 0.0
7.122 ± 0.0
2.878 ± 0.0
4.488 ± 0.0
8.0 ± 0.0
5.756 ± 0.0
8.488 ± 0.0
0.829 ± 0.0
4.244 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here