Lake Sarah-associated circular virus-17
Average proteome isoelectric point is 7.15
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A140AQL3|A0A140AQL3_9VIRU Coat protein OS=Lake Sarah-associated circular virus-17 OX=1685743 PE=4 SV=1
MM1 pKa = 7.89 ANPTRR6 pKa = 11.84 QNARR10 pKa = 11.84 HH11 pKa = 6.17 WIVTQHH17 pKa = 7.19 DD18 pKa = 3.35 IDD20 pKa = 4.2 AFWRR24 pKa = 11.84 GDD26 pKa = 3.61 PTGHH30 pKa = 6.82 HH31 pKa = 6.74 AIKK34 pKa = 10.8 YY35 pKa = 9.58 LVFQVEE41 pKa = 4.34 QGAGTSAWHH50 pKa = 5.47 IQGFIQFKK58 pKa = 7.61 TCVRR62 pKa = 11.84 GSVVQRR68 pKa = 11.84 LFGGNKK74 pKa = 7.78 PHH76 pKa = 7.5 VEE78 pKa = 4.36 VAHH81 pKa = 6.58 HH82 pKa = 6.6 PSQARR87 pKa = 11.84 EE88 pKa = 3.82 YY89 pKa = 10.9 CMKK92 pKa = 10.33 EE93 pKa = 3.98 DD94 pKa = 3.89 TQVTPPEE101 pKa = 4.18 EE102 pKa = 4.24 FGTWEE107 pKa = 3.7 PTFAQGYY114 pKa = 9.45 RR115 pKa = 11.84 SDD117 pKa = 3.67 LTVAKK122 pKa = 10.25 LQIRR126 pKa = 11.84 QHH128 pKa = 5.09 GNYY131 pKa = 9.11 RR132 pKa = 11.84 RR133 pKa = 11.84 CLDD136 pKa = 4.36 DD137 pKa = 5.54 DD138 pKa = 4.68 ALDD141 pKa = 4.03 TVTARR146 pKa = 11.84 YY147 pKa = 7.25 PKK149 pKa = 9.67 WVADD153 pKa = 3.73 QLTMVPRR160 pKa = 11.84 PLRR163 pKa = 11.84 PIPIVTVYY171 pKa = 10.55 YY172 pKa = 10.93 GPTNTGKK179 pKa = 8.12 TARR182 pKa = 11.84 CHH184 pKa = 5.77 INNPGIHH191 pKa = 7.34 EE192 pKa = 4.08 IRR194 pKa = 11.84 LDD196 pKa = 3.55 NGFINYY202 pKa = 7.76 TGQSCVLFDD211 pKa = 5.44 EE212 pKa = 4.98 FDD214 pKa = 3.65 KK215 pKa = 11.42 DD216 pKa = 3.31 PWPFGTMLKK225 pKa = 10.73 LLDD228 pKa = 4.57 RR229 pKa = 11.84 YY230 pKa = 9.83 PFQINVKK237 pKa = 10.1 NGYY240 pKa = 7.66 SWWEE244 pKa = 3.64 ATHH247 pKa = 6.97 IFITATEE254 pKa = 4.71 APCDD258 pKa = 3.37 WYY260 pKa = 11.11 LGKK263 pKa = 10.71 KK264 pKa = 9.02 GVNSDD269 pKa = 5.31 HH270 pKa = 6.89 IPQFLRR276 pKa = 11.84 RR277 pKa = 11.84 LTNVVNTTGSVLPPPLPLSPPTLPVPPSPEE307 pKa = 3.35 IEE309 pKa = 4.25 EE310 pKa = 4.18 QQEE313 pKa = 4.1 EE314 pKa = 4.51 ATMEE318 pKa = 4.5 LDD320 pKa = 4.96 DD321 pKa = 4.16 PTNPEE326 pKa = 3.98 DD327 pKa = 3.87 YY328 pKa = 10.34 EE329 pKa = 4.36 NSEE332 pKa = 3.98 EE333 pKa = 4.24 RR334 pKa = 11.84 DD335 pKa = 3.34 LRR337 pKa = 11.84 EE338 pKa = 3.58 EE339 pKa = 3.78 MEE341 pKa = 5.14 YY342 pKa = 10.67 RR343 pKa = 11.84 DD344 pKa = 3.9 QYY346 pKa = 11.39 QGDD349 pKa = 4.01 SEE351 pKa = 4.88 EE352 pKa = 4.39 YY353 pKa = 10.4
Molecular weight: 40.62 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.061
IPC2_protein 5.105
IPC_protein 5.092
Toseland 5.143
ProMoST 5.27
Dawson 5.143
Bjellqvist 5.245
Wikipedia 5.029
Rodwell 5.067
Grimsley 5.092
Solomon 5.13
Lehninger 5.105
Nozaki 5.283
DTASelect 5.448
Thurlkill 5.169
EMBOSS 5.13
Sillero 5.359
Patrickios 3.745
IPC_peptide 5.143
IPC2_peptide 5.359
IPC2.peptide.svr19 5.3
Protein with the highest isoelectric point:
>tr|A0A140AQL3|A0A140AQL3_9VIRU Coat protein OS=Lake Sarah-associated circular virus-17 OX=1685743 PE=4 SV=1
MM1 pKa = 7.33 KK2 pKa = 10.17 RR3 pKa = 11.84 RR4 pKa = 11.84 YY5 pKa = 9.77 SSSNLSKK12 pKa = 10.78 PFTPPRR18 pKa = 11.84 KK19 pKa = 9.15 LARR22 pKa = 11.84 TTVSVRR28 pKa = 11.84 KK29 pKa = 9.49 PMLQRR34 pKa = 11.84 TMRR37 pKa = 11.84 MTMPRR42 pKa = 11.84 TEE44 pKa = 4.3 LKK46 pKa = 10.44 QVDD49 pKa = 4.2 VVNPSGGATQAVFPLNSTPSIIPVNLITTGSSAWNRR85 pKa = 11.84 IGRR88 pKa = 11.84 KK89 pKa = 9.45 VNLHH93 pKa = 5.63 SCQIQGFFQVNLTTDD108 pKa = 3.03 HH109 pKa = 7.3 DD110 pKa = 5.3 SNVPSWARR118 pKa = 11.84 IMLVYY123 pKa = 10.48 DD124 pKa = 4.11 RR125 pKa = 11.84 QPNGATPNINDD136 pKa = 3.61 ILLSQVNSATDD147 pKa = 3.76 SNVSTPFSATNMNNRR162 pKa = 11.84 DD163 pKa = 3.39 RR164 pKa = 11.84 FEE166 pKa = 4.82 IIRR169 pKa = 11.84 DD170 pKa = 3.26 KK171 pKa = 10.93 RR172 pKa = 11.84 FCLGSIPLANDD183 pKa = 3.19 GQYY186 pKa = 11.53 AVGGFEE192 pKa = 4.62 NSFSVNMYY200 pKa = 9.45 VRR202 pKa = 11.84 LANRR206 pKa = 11.84 EE207 pKa = 4.13 TCFKK211 pKa = 10.58 ADD213 pKa = 3.6 SSPGVIGDD221 pKa = 3.63 IATGSLLFITFGNVISAEE239 pKa = 4.11 NPFDD243 pKa = 3.43 FAGTVRR249 pKa = 11.84 VRR251 pKa = 11.84 YY252 pKa = 10.11 ADD254 pKa = 3.34 VV255 pKa = 3.19
Molecular weight: 28.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.244
IPC2_protein 9.268
IPC_protein 9.531
Toseland 10.028
ProMoST 9.75
Dawson 10.233
Bjellqvist 9.955
Wikipedia 10.423
Rodwell 10.482
Grimsley 10.306
Solomon 10.292
Lehninger 10.248
Nozaki 10.087
DTASelect 9.926
Thurlkill 10.087
EMBOSS 10.438
Sillero 10.16
Patrickios 10.072
IPC_peptide 10.292
IPC2_peptide 8.873
IPC2.peptide.svr19 8.248
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
608
255
353
304.0
34.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.592 ± 0.42
1.48 ± 0.187
5.921 ± 0.507
5.099 ± 1.932
4.605 ± 0.545
6.086 ± 0.125
2.467 ± 1.036
5.263 ± 0.381
3.289 ± 0.094
6.25 ± 0.015
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.303 ± 0.514
6.25 ± 1.464
7.73 ± 0.896
4.77 ± 0.764
6.743 ± 0.677
6.086 ± 2.29
7.895 ± 0.032
7.072 ± 0.716
1.809 ± 0.631
3.289 ± 0.818
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here