Lake Sarah-associated circular virus-17

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A140AQL3|A0A140AQL3_9VIRU Coat protein OS=Lake Sarah-associated circular virus-17 OX=1685743 PE=4 SV=1
MM1 pKa = 7.89ANPTRR6 pKa = 11.84QNARR10 pKa = 11.84HH11 pKa = 6.17WIVTQHH17 pKa = 7.19DD18 pKa = 3.35IDD20 pKa = 4.2AFWRR24 pKa = 11.84GDD26 pKa = 3.61PTGHH30 pKa = 6.82HH31 pKa = 6.74AIKK34 pKa = 10.8YY35 pKa = 9.58LVFQVEE41 pKa = 4.34QGAGTSAWHH50 pKa = 5.47IQGFIQFKK58 pKa = 7.61TCVRR62 pKa = 11.84GSVVQRR68 pKa = 11.84LFGGNKK74 pKa = 7.78PHH76 pKa = 7.5VEE78 pKa = 4.36VAHH81 pKa = 6.58HH82 pKa = 6.6PSQARR87 pKa = 11.84EE88 pKa = 3.82YY89 pKa = 10.9CMKK92 pKa = 10.33EE93 pKa = 3.98DD94 pKa = 3.89TQVTPPEE101 pKa = 4.18EE102 pKa = 4.24FGTWEE107 pKa = 3.7PTFAQGYY114 pKa = 9.45RR115 pKa = 11.84SDD117 pKa = 3.67LTVAKK122 pKa = 10.25LQIRR126 pKa = 11.84QHH128 pKa = 5.09GNYY131 pKa = 9.11RR132 pKa = 11.84RR133 pKa = 11.84CLDD136 pKa = 4.36DD137 pKa = 5.54DD138 pKa = 4.68ALDD141 pKa = 4.03TVTARR146 pKa = 11.84YY147 pKa = 7.25PKK149 pKa = 9.67WVADD153 pKa = 3.73QLTMVPRR160 pKa = 11.84PLRR163 pKa = 11.84PIPIVTVYY171 pKa = 10.55YY172 pKa = 10.93GPTNTGKK179 pKa = 8.12TARR182 pKa = 11.84CHH184 pKa = 5.77INNPGIHH191 pKa = 7.34EE192 pKa = 4.08IRR194 pKa = 11.84LDD196 pKa = 3.55NGFINYY202 pKa = 7.76TGQSCVLFDD211 pKa = 5.44EE212 pKa = 4.98FDD214 pKa = 3.65KK215 pKa = 11.42DD216 pKa = 3.31PWPFGTMLKK225 pKa = 10.73LLDD228 pKa = 4.57RR229 pKa = 11.84YY230 pKa = 9.83PFQINVKK237 pKa = 10.1NGYY240 pKa = 7.66SWWEE244 pKa = 3.64ATHH247 pKa = 6.97IFITATEE254 pKa = 4.71APCDD258 pKa = 3.37WYY260 pKa = 11.11LGKK263 pKa = 10.71KK264 pKa = 9.02GVNSDD269 pKa = 5.31HH270 pKa = 6.89IPQFLRR276 pKa = 11.84RR277 pKa = 11.84LTNVVNTTGSVLPPPLPLSPPTLPVPPSPEE307 pKa = 3.35IEE309 pKa = 4.25EE310 pKa = 4.18QQEE313 pKa = 4.1EE314 pKa = 4.51ATMEE318 pKa = 4.5LDD320 pKa = 4.96DD321 pKa = 4.16PTNPEE326 pKa = 3.98DD327 pKa = 3.87YY328 pKa = 10.34EE329 pKa = 4.36NSEE332 pKa = 3.98EE333 pKa = 4.24RR334 pKa = 11.84DD335 pKa = 3.34LRR337 pKa = 11.84EE338 pKa = 3.58EE339 pKa = 3.78MEE341 pKa = 5.14YY342 pKa = 10.67RR343 pKa = 11.84DD344 pKa = 3.9QYY346 pKa = 11.39QGDD349 pKa = 4.01SEE351 pKa = 4.88EE352 pKa = 4.39YY353 pKa = 10.4

Molecular weight:
40.62 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A140AQL3|A0A140AQL3_9VIRU Coat protein OS=Lake Sarah-associated circular virus-17 OX=1685743 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.17RR3 pKa = 11.84RR4 pKa = 11.84YY5 pKa = 9.77SSSNLSKK12 pKa = 10.78PFTPPRR18 pKa = 11.84KK19 pKa = 9.15LARR22 pKa = 11.84TTVSVRR28 pKa = 11.84KK29 pKa = 9.49PMLQRR34 pKa = 11.84TMRR37 pKa = 11.84MTMPRR42 pKa = 11.84TEE44 pKa = 4.3LKK46 pKa = 10.44QVDD49 pKa = 4.2VVNPSGGATQAVFPLNSTPSIIPVNLITTGSSAWNRR85 pKa = 11.84IGRR88 pKa = 11.84KK89 pKa = 9.45VNLHH93 pKa = 5.63SCQIQGFFQVNLTTDD108 pKa = 3.03HH109 pKa = 7.3DD110 pKa = 5.3SNVPSWARR118 pKa = 11.84IMLVYY123 pKa = 10.48DD124 pKa = 4.11RR125 pKa = 11.84QPNGATPNINDD136 pKa = 3.61ILLSQVNSATDD147 pKa = 3.76SNVSTPFSATNMNNRR162 pKa = 11.84DD163 pKa = 3.39RR164 pKa = 11.84FEE166 pKa = 4.82IIRR169 pKa = 11.84DD170 pKa = 3.26KK171 pKa = 10.93RR172 pKa = 11.84FCLGSIPLANDD183 pKa = 3.19GQYY186 pKa = 11.53AVGGFEE192 pKa = 4.62NSFSVNMYY200 pKa = 9.45VRR202 pKa = 11.84LANRR206 pKa = 11.84EE207 pKa = 4.13TCFKK211 pKa = 10.58ADD213 pKa = 3.6SSPGVIGDD221 pKa = 3.63IATGSLLFITFGNVISAEE239 pKa = 4.11NPFDD243 pKa = 3.43FAGTVRR249 pKa = 11.84VRR251 pKa = 11.84YY252 pKa = 10.11ADD254 pKa = 3.34VV255 pKa = 3.19

Molecular weight:
28.19 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

608

255

353

304.0

34.4

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.592 ± 0.42

1.48 ± 0.187

5.921 ± 0.507

5.099 ± 1.932

4.605 ± 0.545

6.086 ± 0.125

2.467 ± 1.036

5.263 ± 0.381

3.289 ± 0.094

6.25 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.303 ± 0.514

6.25 ± 1.464

7.73 ± 0.896

4.77 ± 0.764

6.743 ± 0.677

6.086 ± 2.29

7.895 ± 0.032

7.072 ± 0.716

1.809 ± 0.631

3.289 ± 0.818

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski