Wuhan spider virus 5

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KJ82|A0A1L3KJ82_9VIRU Uncharacterized protein OS=Wuhan spider virus 5 OX=1923754 PE=4 SV=1
MM1 pKa = 7.37ATPIAQANSSVSNGLDD17 pKa = 3.1LPSTVEE23 pKa = 4.2AGAHH27 pKa = 5.8LPSEE31 pKa = 4.59IQTPTPPTVEE41 pKa = 4.09VPFAAGEE48 pKa = 4.21GNRR51 pKa = 11.84LDD53 pKa = 3.68PYY55 pKa = 10.64FYY57 pKa = 10.45EE58 pKa = 3.96QFIKK62 pKa = 11.1VEE64 pKa = 4.08VFPWTTMDD72 pKa = 3.41QPGKK76 pKa = 10.61VLFVLKK82 pKa = 10.56LDD84 pKa = 3.98PKK86 pKa = 10.86NINKK90 pKa = 9.01QLAYY94 pKa = 10.58FLDD97 pKa = 4.85AYY99 pKa = 8.87YY100 pKa = 11.18AWGGDD105 pKa = 2.87IKK107 pKa = 11.22LLLKK111 pKa = 10.86VLGTSFHH118 pKa = 6.83AGQLGIYY125 pKa = 7.56YY126 pKa = 9.38LPPGVDD132 pKa = 3.34YY133 pKa = 11.33KK134 pKa = 11.47NYY136 pKa = 9.3TPQEE140 pKa = 3.76LSIFPWEE147 pKa = 4.07IVDD150 pKa = 3.73VKK152 pKa = 10.84DD153 pKa = 3.9INLHH157 pKa = 5.91EE158 pKa = 4.32ILMRR162 pKa = 11.84DD163 pKa = 3.13IRR165 pKa = 11.84PTKK168 pKa = 10.43YY169 pKa = 10.37HH170 pKa = 5.75MLEE173 pKa = 4.88DD174 pKa = 3.88KK175 pKa = 10.96PNDD178 pKa = 3.51PFYY181 pKa = 10.33VQSGGTLVIAADD193 pKa = 4.13TQLSTSSTGVNKK205 pKa = 10.02IYY207 pKa = 10.85VSVWSKK213 pKa = 10.39LAEE216 pKa = 4.26NFQVAYY222 pKa = 8.11MIPPRR227 pKa = 11.84EE228 pKa = 4.36LKK230 pKa = 10.54PSLNFSNEE238 pKa = 2.79FMAAVNSIFYY248 pKa = 9.63TGSKK252 pKa = 9.98SLASAPFRR260 pKa = 11.84IDD262 pKa = 3.3TIDD265 pKa = 3.47IQPVTIRR272 pKa = 11.84QTFNGIYY279 pKa = 10.4NCFTTAGEE287 pKa = 4.1NLYY290 pKa = 9.78TPILPDD296 pKa = 4.63DD297 pKa = 4.07YY298 pKa = 11.27PNSKK302 pKa = 10.25FNLPIYY308 pKa = 10.19PMAGTFRR315 pKa = 11.84TTSNPFEE322 pKa = 4.18VEE324 pKa = 3.39ISFGFWEE331 pKa = 5.2FWPKK335 pKa = 10.74EE336 pKa = 3.75FTISYY341 pKa = 10.37KK342 pKa = 11.01DD343 pKa = 3.26GDD345 pKa = 4.11KK346 pKa = 10.21RR347 pKa = 11.84TSATPSDD354 pKa = 3.92MSWDD358 pKa = 3.28KK359 pKa = 11.52SDD361 pKa = 3.54PFKK364 pKa = 11.44VSFTHH369 pKa = 7.48EE370 pKa = 4.77DD371 pKa = 3.63GLSVKK376 pKa = 9.73IDD378 pKa = 3.55EE379 pKa = 4.59PVKK382 pKa = 10.45FFPDD386 pKa = 3.28NVGVFTDD393 pKa = 3.78KK394 pKa = 10.89VSPVNQSTTPVNTPQARR411 pKa = 11.84EE412 pKa = 4.1SIVYY416 pKa = 7.68FQNSFGAYY424 pKa = 8.49SLQPAVLAQACADD437 pKa = 3.58KK438 pKa = 11.18QFIDD442 pKa = 4.96VIPQGMAALLIMTEE456 pKa = 4.18KK457 pKa = 10.45TSGLPLTYY465 pKa = 10.5LKK467 pKa = 10.44FYY469 pKa = 10.84NRR471 pKa = 11.84GIFTAPASVDD481 pKa = 3.64LVKK484 pKa = 10.7FSLDD488 pKa = 3.24DD489 pKa = 3.77KK490 pKa = 11.1QIKK493 pKa = 9.8FSNFVLEE500 pKa = 4.49TQSFPVNEE508 pKa = 3.84EE509 pKa = 3.8MIRR512 pKa = 11.84NMLAYY517 pKa = 10.5CSFHH521 pKa = 5.72TNKK524 pKa = 10.53KK525 pKa = 7.78IMRR528 pKa = 11.84RR529 pKa = 11.84ISSAKK534 pKa = 9.72SLQSRR539 pKa = 11.84KK540 pKa = 9.55ALKK543 pKa = 10.57KK544 pKa = 9.99FVTFQEE550 pKa = 4.32TGII553 pKa = 4.1

Molecular weight: 62.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KJ39|A0A1L3KJ39_9VIRU Calici_coat domain-containing protein OS=Wuhan spider virus 5 OX=1923754 PE=4 SV=1
MM1 pKa = 7.47ALVALGAAALAGGISAGATLGASLGAADD29 pKa = 4.37VQSKK33 pKa = 7.79TATSINNQNLDD44 pKa = 3.53FAKK47 pKa = 10.02QQYY50 pKa = 10.35SEE52 pKa = 4.32GLSSFKK58 pKa = 10.51QAGLPSFLYY67 pKa = 10.9YY68 pKa = 10.69SGGNSGLGNLPKK80 pKa = 9.58TQSHH84 pKa = 6.52IEE86 pKa = 4.01GSSFSSSLGVNADD99 pKa = 3.5LPYY102 pKa = 11.11YY103 pKa = 10.09SANPYY108 pKa = 9.02NQYY111 pKa = 10.52LQTGRR116 pKa = 11.84PVPTNKK122 pKa = 8.16QTQEE126 pKa = 4.26RR127 pKa = 11.84IPNSNNNTPRR137 pKa = 11.84SNDD140 pKa = 2.8IEE142 pKa = 4.22LQDD145 pKa = 4.07FSSIEE150 pKa = 3.78PTSRR154 pKa = 11.84ITNSNGNNFQYY165 pKa = 10.86GGSTSMDD172 pKa = 3.27NLRR175 pKa = 11.84AQFAPMLGGNRR186 pKa = 11.84QSGSGKK192 pKa = 9.84YY193 pKa = 10.37SSVPPPASYY202 pKa = 7.93FTSNRR207 pKa = 11.84YY208 pKa = 8.78SQTPPNFGNVSKK220 pKa = 10.87SSQTSYY226 pKa = 11.32SSNFGSNSATNNTRR240 pKa = 11.84SFYY243 pKa = 10.83SQTNGIFFRR252 pKa = 11.84SVGFNPLSARR262 pKa = 11.84GRR264 pKa = 3.68

Molecular weight: 27.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

3565

218

2530

891.3

100.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.61 ± 0.792

1.683 ± 0.463

5.75 ± 0.621

5.891 ± 0.717

5.217 ± 0.54

4.769 ± 0.822

2.328 ± 0.674

6.396 ± 0.819

6.76 ± 0.68

8.387 ± 0.34

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.384 ± 0.183

5.806 ± 0.853

5.61 ± 0.581

3.506 ± 0.682

4.039 ± 0.392

8.527 ± 1.314

5.666 ± 0.446

6.424 ± 0.655

0.954 ± 0.209

4.292 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski